Article Per Year (5 Year)
p-Index From 2020 - 2025
2.162
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran Syiah Kuala International Journal of Integrated Health Sciences Pharmaciana: Jurnal Kefarmasian Media Ilmu Keolahragaan Indonesia Buletin Farmatera Jurnal Keperawatan dan Fisioterapi (JKF) Jurnal Kesmas dan Gizi (JKG) IJOEP : International Journal of Ecophysiology Narra J
Yamamoto, Zulham
Department Of Histology, Faculty Of Medicine, Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

Published : 11 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 11 Documents
Search

 1   2 
Direct PCR for Escherichia coli: a straightforward and cost-effective method Harahap, Desy Aryani; Yamamoto, Zulham; Widjaja, Sry Suryani; Mayasari, Evita
JURNAL KESMAS DAN GIZI (JKG) Vol. 7 No. 2 (2025): Jurnal Kesmas dan Gizi (JKG)
Publisher : Fakultas Kesehatan Masyarakat Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jkg.v7i2.2606

Abstract

This study compares the direct PCR method with standard PCR to detect Escherichia coli. Escherichia coli is the most widely used organism in biology. Detection of E. coli in water and food is routinely performed, utilizing methods like PCR. The initial stage of PCR preparation involves DNA extraction, which requires a commercial kit. Consequently, this extraction process incurs additional expenses, time, and labor. Therefore, an alternative method is needed, such as direct PCR, which can circumvent the need for DNA extraction. The PCR process facilitates lysis of the bacterial cell wall, releasing nucleic acids, which can then be amplified by Taq polymerase. For the PCR procedure, two groups were formed, each comprising three replicates of the reaction with different DNA templates. The first group utilized a direct culture of Escherichia coli, while the second group incorporated the extracted DNA of Escherichia coli. Our study successfully amplified the metH gene of Escherichia coli without DNA extraction. Electrophoresis analysis revealed that the direct PCR product, sized at 300 bp, appeared more pronounced than the standard PCR product. The findings of this research demonstrated direct PCR as an alternative for detecting Escherichia coli, which would lead to reductions in costs, time, and labor.
Co-Authors Amin Soebandrio Bambang Prayugo Bayu Dewanto Dina Rahmi Solihad Nasution Evirosa Juliartha Simanjuntak Gembira Arkemo Situmorang Harahap, Desy Aryani Jelita Siregar Kevin Kevin Kusumawati, R. Lia M. Ichwan Mayasari, Evita Muhammad Ichwan Muhammad Ichwan Novrina Situmorang Nuraiza Meutia Pandia, Pandiaman Rahmi Rahmi Rambe, Ismatul Fauziah Rina Yunita Sembiring, Rosita J. Sinaga, Bintang YM. Syahfira A Syahna Wahyuni, Arlinda S. Widjaja, Sry Suryani Yetty Machrina