Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.064

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Jurnal Sain Peternakan Indonesia BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Tropical Animal Science Journal Indonesian Aquaculture Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Aquacultura Indonesiana Journal of Tropical Ethnobiology Unri Conference Series: Community Engagement International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Makara Journal of Science

Abinawanto Abinawanto

Departemen Biologi, Universitas Indonesia

Author-ID : 218331

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Immunology & microbiology Medicine & Pharmacology Veterinary Other

Published : 28 Documents Claim Missing Document

Claim Missing Document

Articles

Title

Screening of Actinomycetes Producing an ATPase Inhibitor of Japanese Encephalitis Virus RNA Helicase from Soil and Leaf Litter Samples SHANTI RATNAKOMALA; RONI RIDWAN; PUSPITA LISDIYANTI; . ABINAWANTO; UTAMA ANDI
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Actinomycetes are commercially important microorganisms for the production of antibiotics, enzymes, inhibitors of enzymes, and other bioactive secondary metabolites. Some 853 isolates of actinomycetes were isolated from soil and leaf litter samples in Kupang NTT and Enrekang, South Sulawesi. Those isolates were then tested for inhibition of ATPase activity of RNA helicase from Japanese encephalitis virus (JEV), in order to identify a drug candidate for the treatment of JEV infection. Results revealed that 14 isolates have relatively high inhibition-activity on JEV ATPase activity of the JEV-RNA-helicase, which range from approximately 40.0-50.0% inhibition. The highest inhibition-activity was identified in Actinoplanes philippinensis 5-849 with 49. 9% of inhibition-activity and Streptomyces chartreusis 5-095 with 49.2% of inhibition-activity

Cloning of Lipase Gene From Thermomyces langinosus into Pichia pastoris with its Original Signal Peptide MILANI ANGGIANI; IS HELIANTI; NIKNIK NURHAYATI; ABINAWANTO ABINAWANTO
Microbiology Indonesia Vol. 11 No. 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

Lipase is one of the most important industrial enzymes, which is widely used in the preparation of food additives, cosmetics, and pharmaceutical industries. In the previous study, we have cloned synthetic Thermomyces lanuginosus lipase gene into Bacillus subtilis and Escherichia coli and resulting low expression for enzyme activity. The aim of this research was to construct the Thermomyces lanuginosus lipase (TLL) gene into Pichia pastoris vector expression with TLL original signal peptide. TLL gene was amplified by PCR and contained original signal peptide and then inserted into pPICZÎ± A between XhoI and XbaI site, and transformed into competent cell E.coli DH5Î±. From the transformant, two of positive recombinants were analyzed by sequencing analysis. As the result,both of two recombinant have a positive target gene which has lipase gene. The correct plasmid was linearized and then was transformed in Pichia pastoris X-33 by electroporation method. Thermomyces lanuginosus synthetic gene lipase has been successfully integrated into chromosome of P. pastoris X-33, which revealed by clear zones arund the colony on Yeast extract Peptone Dextrose Tributyrin (YPD.TB) plate with zeocin. The Thermomyces lanuginosus lipase had an open reading frame of 916bp encoding TLL of 314 amino acids with theoretical molecular mass of 35 kDa. The recombinant enzyme, Thermomyces lanuginosus lipase had optimal temperature at 80ËšC and optimal pH at pH 8.

Cloning and expression of NS2B/NS3 protein of DENV3 Indonesia strain in Saccharomyces cerevisiae expression system for the development of Dengue vaccine ASRI SULFIANTI; SABAR PAMBUDI; ISMA NUR AZIZAH; DODDY IRAWAN SETYO UTOMO; ABINAWANTO ABINAWANTO
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

NS3 protein is 618 amino acids (aa) in length containing serine protease and helicase domains required for DENV replication. Alignment of consensus amino acid sequences from all four DENV serotypes demonstrated that this protein is more conserved (78%) among the different dengue serotypes, which elicits a strong cellular immune response after viral infection in humans and animal models. Present study, a central hydrophilic region of NS3 cofactor, NS2B (NS2BH) with full length of NS3 genes DENV3 Indonesian strain were amplified from CDNA following PCR, and inserted to PYES2/CT shuttle vector. The recombinant plasmid was transformed and expressed in Saccharomyces cerevisiae expression system. As result, detection with Anti-His detector and Anti-NS3 shown NS2BH/NS3 was expressed as 83 KDA protein band. We found that addition of NS2BH on NS3 full length construction plasmid increase the yield and activity of protein expression in S. cerevisiae. In future study, our recombinant NS2B/NS3 protein can be used as recombinant protein in Dengue vaccine development.

Isolation of a Functional Gene Encoding Homologous Lysophospholipase from Indonesian Indigenous Bacillus halodurans CM1 SHANNI FERNANDA; ABINAWANTO ABINAWANTO; IS HELIANTI
Microbiology Indonesia Vol. 13 No. 1 (2019): March 2019
Publisher : Indonesian Society for microbiology

Lipase is a biocatalyst widely used in industry, for example detergent, pharmaceutical, food, or oil purification. One of the most widely lipase used for oil purification is lysophospholipase. As much as 50% of industrial enzyme needs are supplied from microorganisms. However, enzyme productivity from wild type microbial strain is usually limited and not applicable in industry, so that genetic engineering is necessary. Cloning gene encoding for lysophospholipase from Aspergillus niger and Cryptococcus neoformans have been conducted, but has never been conducted from alkalothermophilic bacteria, such as Bacillus halodurans. Bacillus halodurans CM1 is an alkalothermophilic bacterial strain isolated previously that has many industrially potential enzymes. This study aimed to isolate one of the gene encoding lipase from Bacillus halodurans CM1 and cloned into Escherichia coli DH5Î± using the pGEM-T easy vector. The gene fragment encoding lysophospholipase obtained with size 783 base pairs and had 100% similarity with gene encoding lysophospholipase from Bacillus halodurans C-125 (No access GenBank: BA000004.3). E. coli harbouring the recombinant plasmid with the gene also showed activity on trybutiryn medium compared to negative control.

Co-Authors A. Sofyan A. Sofyan Abdul Basith Achmad Dinoto Achmad Dinoto Achnafani, Dini Ade Sunarma Agung Sudaryono Agus Haryadi Ahmad Aulia Jusuf Alimuddin Andi Salamah ANOM BOWOLAKSONO Arief Boediono ASRI SULFIANTI Bayu , Mariana D. Diki Nurul Huda Djamhuriyah S. Said DODDY IRAWAN SETYO UTOMO Dwiranti, Astari Endang C. Purba Endang Sukara Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini, Eni Erma Primanita Hayuningtyas Fitrianingrum, Nisa Funahashi, Hiroaki H. Julendra Hidayat Hidayat Huriyah Adani Saoemi is helianti IS HELIANTI IS HELIANTI Isma Nur Azzizah Ita M. Nainggolan L. Istiqomah Lina Mulyawati Lulut Dwi Sulistyaningsih M. F. Karimy M. F. Karimy Maria Ulfah Maria Ulfah Mariana D. Bayu Marina Silalahi Marlina Ardiyani Maylasari, Roospita MILANI ANGGIANI Nataniel, Jocelyn NIKNIK NURHAYATI NIKNIK NURHAYATI Nisyawati Nova Anita Noviyanti, Rintis Nurfitriani, Risya Odang Carman Prakoso, Nurul Muhammad PUSPITA LISDIYANTI Putra Mahanaim Tampubolon Rabbil Pratama Aji Retno Lestari Retno Lestari Retno Lestari Retno Lestari Riska S. Wahyuningtyas Rita Rostika RONI RIDWAN Sabar Pambudi Sabbathini, Gabriela Christy Sanya Khaerunnisa Sawung Cindelaras, Sawung SHANNI FERNANDA Shanti Ratnakomala Siti Z Musthofa Solly Aryza Sulistiani ., Sulistiani Sulistiani Sulistiani Sulistiani Sulistiani, Sulistiani Sunarma , Ade Sundari, Ayu Mulia Taniawati Supali Trianti, Lely Uswatun Hasanah UTAMA ANDI Valerry Athalia Priyanka Wibowo Mangunwardoyo WIBOWO MANGUNWARDOYO Wulandari, Sri Rezeki Wulandhary, Sendy Yasman Yasman Yasman Yushinta Fujaya

Title Search

Found 4 Documents Search Journal : Microbiology Indonesia

Abstract

Abstract

Abstract

Abstract

Title

Found 4 Documents
Search
Journal : Microbiology Indonesia