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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Jurnal Sain Peternakan Indonesia BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Tropical Animal Science Journal Indonesian Aquaculture Journal Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Aquacultura Indonesiana Journal of Tropical Ethnobiology Unri Conference Series: Community Engagement International Journal of Islamic Education, Research and Multiculturalism (IJIERM) Makara Journal of Science

Abinawanto Abinawanto

Departemen Biologi, Universitas Indonesia

Author-ID : 218331

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Immunology & microbiology Medicine & Pharmacology Veterinary Other

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STUDI KROMOSOM IKAN PELANGI (Melanotaenia lacustris) [Chromosome Study of Rainbowfish (Melanotaenia lacustris)] Djamhuriyah S. Said; Odang Carman; Hidayat Hidayat; Abinawanto Abinawanto
Jurnal Iktiologi Indonesia Vol 3 No 2 (2003): Desember 2003
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v3i2.262

Cytogenetic study of Melanotaenia lacustris was focussing on karyotype and to find out the information of chromosome. The research was conducted in Laboratory of Fish Breeding and Genetics, Faculty of Fisheries and Marine Science-IPB on May==December 2000. Chromosome plates were prepared by solid tissue technique and analyzed after staining with giemsa solution. Diploid chromosomes number of M. lacustris are (2n = 46). Karyotyping of this fish shown that 46 chromosomes consist of 9 pairs submetacentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pairs subtelocentric (ST) (no. 4, 12, 22) and 10 pairs telocentric (T) (no. 2, 5, 11, 15, 16, 17, 18, 19, 20, and 21) with 1 ST and 1 T on the no. 23. Therefor is suggested that M. lacustris has a sex chromosome.AbstrakPenelitian sitogenetika pada ikan pelangi (Melanotaenia lacustris) difokuskan pada pengungkapan keanekaragaman kromosom dalam hal jumlah, bentuk, dan karyotipenya. Penelitian dilakukan di Laboratorium Genetika dan Reproduksi ikan Fakuitas Perikanan dan 11 mu Kelautan IPB, pada bulan on Mei — Deseber 2000. Preparat kromosom dibuat dengan metode jaringan padat yang menggunakan larva ikan umur 10-30 hari. Analisis kromosom dilakukan setelah pewarnaan dengan larutan Giemza. Kromosom diploid ikan M. lacustris adalah (2N = 46). Karyotipenya menunjukkan 46 kromosom yang terdiri atas 9 pasang berbentuk submetasentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pasang subtelosentrik (ST) (no. 4, 12, 22) dan 10 pasang telosentrik (T) (no. 2, 5, 11, 15, 16, 17, IB, 19, 20, and 21) dengan 1 ST dan 1 T pada no. 23. Dari basil tersebut diduga bahwa M, lacustris memiliki kromosom sek.

The honey solution effect as a natural cryoprotectant on sperm quality of botia, Chromobotia macracanthus Bleeker 1852 Abinawanto Abinawanto; Siti Z Musthofa; Retno Lestari; Anom Bowolaksono
Jurnal Iktiologi Indonesia Vol 20 No 3 (2020): October 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i3.528

The purpose of study was to obtain the best combination of natural cryoprotectant (honey solution) and synthetic cryoprotectant (methanol) on the quality of spermatozoa Chromobotia macracanthus Bleeker 1852, after freezing at -80 ℃ for 48 hours. The combination of 10% methanol with various concentrations of honey solution (0%, 0.1%, 0.3%, 0.5%, 0.7% and 0.9%) was tested in this study. Ringer's solution was used as an extender. The diluted sperm was then equilibrated for 25 minutes at 4 ℃, then frozen at -80 ℃ for 48 hours. The sperm was then thawed at 40 ℃ for 13 seconds. Viability, motility and percentage of fertilization were evaluated. The results of the analysis of variance showed that the combination of 10% methanol with several concentrations of honey solution had a significant effect (P <0.05) on the viability of spermatozoa and the percentage of fertilization, but had no effect on sperm motility (P> 0.05). The combination of 0.1% honey solution and 10% methanol showed the highest percentage of both motility (89.4 ± 5.45%), viability (85.75 ± 4.79%), and the percentage of fertilization (98.55 ± 1.69%). %). The conclusion of this study is that 0.1% honey solution combined with 10% methanol in Ringer's solution is the best cryoprotectant for C. macracanthus spermatozoa stored at -80 ℃ for 48 hours. Abstrak Penelitian ini bertujuan untuk mendapatkan kombinasi terbaik antara krioprotektan alami (larutan madu) dan krioprotektan sintetik (metanol) terhadap kualitas spermatozoa Chromobotia macracanthus Bleeker 1852, pasca penyimpanan pada suhu -80 ℃ selama 48 jam. Kombinasi metanol 10% dengan berbagai konsentrasi larutan madu (0%, 0,1%, 0,3%, 0,5%, 0,7% dan 0,9%) diuji dalam penelitian ini. Larutan Ringer digunakan sebagai ekstender. Sperma yang telah diencerkan, kemudian diekuiliberasi selama 25 menit pada suhu 4 ℃, kemudian disimpan beku pada suhu -80 ℃ selama 48 jam. Sperma kemudian dicairkan pada suhu 40 ℃ selama 13 detik. Viabilitas, motilitas dan persentase fertilisasi dievaluasi. Hasil uji analisis sidik ragam menunjukkan bahwa kombinasi metanol 10% dengan beberapa konsentrasi larutan madu berpengaruh secara signifikan (P<0,05) terhadap viabilitas spermatozoa dan persentase fertilisasi, tapi tidak berpengaruh terhadap motilitas spermatozoa (P>0,05). Kombinasi larutan madu 0,1% dan metanol 10% menunjukkan persentase tertinggi baik motilitas (89,4 ± 5,45%), viabilitas (85,75 ± 4,79%), maupun persentase fertilisasi (98,55 ± 1,69%). Kesimpulan penelitian ini adalah bahwa larutan madu 0,1% yang dikombinasikan dengan metanol 10% dalam larutan Ringer merupakan krioprotektan terbaik bagi spermatozoa C. macracanthus yang disimpan pada suhu -80 ℃ selama 48 jam.

Ethnobotanical Study of Zingiberaceae Rhizomes as Traditional Medicine Ingredients by Medicinal Plant Traders in the Pancur Batu Traditional Market, North Sumatera, Indonesia Marina Silalahi; Nisyawati; Endang C. Purba; Daichiro W. Abinawanto; Riska S. Wahyuningtyas
Journal of Tropical Ethnobiology Vol. 4 No. 2 (2021): July 2021
Publisher : The Ethnobiological Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46359/jte.v4i2.54

Traditional markets are places for buying and selling medicinal plants and are a source of ethnobotany research data. This study aims to determine the uses and characteristics of Zingiberaceae rhizomes have been used as traditional medicine and traded in the traditional market of Pancur Batu, North Sumatra. This research was conducted with an ethnobotany approach through surveys, interviews and observation participatory. The respondents are all medicinal plants traders in the Pancur Batu traditional market. The things that were asked of the traders included local names, special characters, benefits, and how to recognize the rhizome. The medicinal plant traders in the Pancur batu traditional market have been utilized and traded as many as 10 species of Zingiberaceae rhizome, most of them belonging Curcuma and Zingiber genera. The characteristics of rhizomes are recognized by traders through their size, color, and aroma. The cross-section of the rhizomes of each species is different in structure and color which is used as the main marker for each species. Rhizoma Zingiberaceae is used as the main ingredient for tawar (semi-solid medicinal herbs consumed by brewing), parem (solid medicinal ingredients), and oukup (traditional Karo sauna). The rhizome aroma of each species belonging Zingiberaceae is very distinctive which is related to the content of essential oils. The use of Zingiberaceae rhizomes as tawar and parem ingredients needs to be studied further so that they are developed into standardized herbs.

Keripik Jamur Sebagai Hilirisasi Produk dari Hasil Budidaya Jamur Tiram (Pleurotus ostreatus) di Desa Bojong Koneng, Sentul, Jawa Barat Retno Lestari; Abinawanto Abinawanto; Sanya Khaerunnisa; Rabbil Pratama Aji; Diki Nurul Huda; Putra Mahanaim Tampubolon
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.310-315

Oyster mushroom cultivation is one of the economic drivers of the Bojong Koneng Village community. Standard operating procedures have been developed through a research process and have been well implemented in carrying out the mushroom cultivation. The development of downstream products from oyster mushroom cultivation harvests that have been carried out has become the focus of community service activities as value added that can improve the economy of the people of Bojong Koneng Village. The product developed as a continuation of the cultivation program is mushroom chips which have a good taste, crunchy texture, and high protein content. Community service activities are carried out through counseling, focus group discussions, mushroom chip making practices, and monitoring. The impact of this activity is able to increase the knowledge and skills of the people of Bojong Koneng Village and it is hoped that it can increase the productivity of the village community for better welfare.

Nutrient Utilizations and Intestinal Morphology of Broilers Treated with Lactobacillus plantarum AKK30 – Oligosaccharides Synbiotic H. Julendra; A. Sofyan; M. F. Karimy; Abinawanto Abinawanto; Yasman Yasman
Tropical Animal Science Journal Vol. 43 No. 2 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Lactobacillus plantarum AKK30 inoculum in de Maan Rogosa Sharpe Broth (MRSB) grown on media containing oligosaccharides (inulin or mannan oligosaccharides/MOS) was studied in vitro and in vivo. In vitro assay was conducted to characterize metabolite profiles and its effect on pathogenic bacteria, while in vivo assay was conducted to study the metabolic energy, nitrogen retention, and intestinal morphology of broilers. A total of 24 male broilers-40-day-old (average body weight= 1.725±0.05 kg) strains Cobb-500 were used, where 20 birds were added 1% inoculum L plantarum AKK30 of daily intake and randomly distributed in five treatments and four replications consisted of: A) MRSB + L. plantarum AKK-30 (10⁷ cfu g-1); B) MRSB + inulin 0.5% (w v-1); C) MRSB + MOS 0.5%(w v-1); D) L. plantarum AKK-30 (10⁷ cfu g-1) + MRSB + inulin 0.5% (w v-1).; and E) L. plantarum AKK-30 (10⁷ cfu g-1) + MRSB + MOS 0.5% (w v-1), while 4 birds were fasted as endogenous chicken. The results showed that the highest isoleucine and threonine were found in group E. The highest oleic acid and the lowest conjugated linoleic acid (CLA) were observed in all treatments and inoculum did not affect antibacterial activity. The metabolizable energy values and nitrogen retention in group D and E were higher than other treatments (p<0.05). The lowest height of villi was observed in group A (p<0.05). In conclusion, the addition of oligosaccharides inulin or mannan oligosaccharide (MOS) affects the metabolite profiles of Lactobacillus plantarum AKK30 and their synbiotic effects improve intestinal morphology, metabolizable energy, and nitrogen retention in broilers.

Intestinal Morphology, Energy Availability, and Growth Performance of Broilers Treated with the Combination of Probiotic and Inulin H. Julendra; A. Sofyan; L. Istiqomah; M. F. Karimy; Abinawanto Abinawanto; Yasman Yasman
Tropical Animal Science Journal Vol. 44 No. 1 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.1.39

Probiotic and prebiotic or their combinations can potentially function as an alternative to antibiotics growth promoters (AGPs) for broiler. This study was designed to investigate the growth performance, intestinal microstructure, and nutrients digestibility of broilers administered with probiotics of Lactobacillus plantarum AKK30 and Saccharomyces cerevisiae B18 in combination with inulin. A total of 275 male chickens (initial bodyweight of 47±0.05 g) were reared for growth performance evaluation. At the 32-d-old, 25 male chickens were necropsied for intestinal microstructural analysis, while the other 25 male chickens were selected for evaluation of digestibility (body weight= 1525±0.08 g). Treatments of probiotics in combination with different levels of inulin consisted of control with probiotics without inulin (S0), probiotics with 0.5% of inulin (S1), probiotics with 1.0% of inulin (S2), probiotics with 1.5% of inulin (S3), and commercial probiotics without inulin (Sc), which were arranged in a completely randomized design with five replications. Results showed that body weight gain and performance index in broilers treated prebiotics in combination with 0.5% inulin (S1), 1% inulin (S2), and commercial probiotic without inulin (Sc) were significantly higher (p<0.05) than those in control broiler chickens that were treated with probiotics without inulin. Feed intake showed no differences among treatments, whereas feed conversion ratios in broiler chickens treated with the commercial probiotics (Sc) or probiotics in combination with inulin at 0.5% (S1) and 1.0% (S2) were lower than control chickens. Metabolizable energy, nitrogen retention, and villi height in chickens treated with probiotics in combination with 0.5% inulin (S1) and 1.0% inulin (S2) were higher than those in the control group. In conclusion, the administration of probiotic combined with inulin at the level of either 0.5 or 1.0% improves broiler performance, intestinal microstructure, and nutrients digestibility.

Conjugational Transformation of Wild Type Bacillus halodurans CM1 by Methylated Recombinant Plasmid Harbouring a Gene Encoding for Alkaline Protease and the Protease Activity Assay of the Transformant Valerry Athalia Priyanka; Maria Ulfah; Lina Mulyawati; Abinawanto; is helianti
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.799-805

Protease contributes significantly to various industrial sectors, as shown by its increasing demand. An indigenous previously isolated bacteria, Bacillus halodurans CM1, is a wild-type bacterium whose ability to produce alkalotermophilic protease. In this study, the transformation of methylated pBBRE194 plasmid containing alkaline protease gene from this strain (PBBRE194-prot-CM1) into itself using a conjugation approach was conducted, and the measurement of the alkaline protease activity of the recombinant bacterium was carried out. The recombinant B. halodurans CM1 has been verified to carry the methylated pBBRE194 prot-CM1 by examining its ability to degrade protein in media containing skim milk and tetracycline, but also by amplifying tetracycline resistance gene sequence with 1,024 bp length of plasmid pBBRE194 prot-CM1 by PCR method from the recombinant bacterium. The results confirmed that recombinant B. halodurans CM1 was positively harboring plasmid pBBRE194 prot-CM1. Alkaline protease produced by recombinant CM1 reached higher activity than wild type between 18-36 h of cultivation. The alkalotermophilic wild type B. halodurans could accept the recombinant plasmid into their cells via conjugational transformation is firstly reported to our further knowledge.

DNA Barcode Characterization of Chocolate Hind Grouper (Cephalopholis boenak) in Several Indonesia Waters with the New Sequences Record from Madura Island Abdul Basith; Abinawanto; Eni Kusrini; Yasman
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.733-741

This study aims to describe the molecular characteristics of DNA barcodes in chocolate hind grouper (Cephalopholis boenak) in Indonesian waters with new sequence records from Madura Island waters. Partial sequences of the cytochrome c oxidase 1 (CO1) gene were successfully obtained from two samples of C. boenak in Madura Island waters. In contrast, the other sequences were obtained from the BOLD system database, covering the Islands of Bali, Lombok, and Ambon waters. The overall molecular analysis involved 10 C. boenak sequences with a length of 625 bp. The results indicated that the population of C. boenak in Indonesia waters has high genetic diversity, as evidenced by the value of haplotype diversity (Hd) = 0.956 and nucleotide sequence diversity (Pi) = 0.01746, in which the population is distributed into eight haplotypes. Results of phylogenetic tree reconstruction of neighbor-joining and maximum-likelihood indicate similar topology. The branching of NJ and ML phylogenetic trees of C. boenak in Indonesia waters is grouped into two geographical clades. Clade 1 with the subpopulations of the waters from Madura, Bali, and Lombok Islands. Clade 2 with the subpopulations of the waters from Ambon Island. The results of the median-joining network reconstruction depicted a similar topology with both phylogenetic trees.

ISOLATION AND IDENTIFICATION OF PARTIAL GROWTH HORMONE (GH) mRNA OF TIGER SHOVELNOSE CATFISH Pseudoplatystoma fasciatum (LINNAEUS, 1766) Erma Primanita Hayuningtyas; Eni Kusrini; Abinawanto Abinawanto
Indonesian Aquaculture Journal Vol 17, No 2 (2022): (December, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.2.2022.107-119

The growth of fish is regulated by growth hormones secreted in a limited amount by its pituitary glands. Tiger shovelnose catfish has a relatively faster growth indicating that some growth hormones suspectedly play roles in the process and could be used in improving other farmed fish species. This study aimed to isolate and identify the growth hormone (GH) mRNA gene in Tiger shovelnose catfish (Pseudoplatystoma fasciatum Linnaeus, 1766). The sample was isolated from the pituitary, the fish measuring 602 g and 43 cm body length; Total RNA was extracted using the Tri Reagent Kit, followed with cDNA synthesis. The success of the extraction was confirmed by quantification and PCR using the β-actin gene as an internal control. GH mRNA gene was isolated by RT-PCR method, with degenerated primers from seven catfish species sequence data in the NCBI gene bank. The single band from GH gene was cloned and sequenced. Total RNA quantification with a concentration of 227 ng/µL and purity of 1.821. The successful isolation of mRNA from the pituitary gland was confirmed by amplifying the β-actin gene generated at 300 bp. This isolation of the GH mRNA gene had a sequence length of 234 bp. Tiger shovelnose catfish GH gene consists of 17 amino acid residues. The GH gene of Tiger shovelnose catfish was close to that of striped catfish (P. hypophthalmus) and Indian catfish (Clarias batrachus) with almost similar homology value of 90.6 %. Partial GH gene from tiger shovelnose catfish can be used a molecular marker in revealing the role of growth hormone on fish development, fish biology, growth gene expression, selective breeding and other mechanisms related to the aquaculture.

Cloning of Lipase Gene From Thermomyces langinosus into Pichia pastoris with its Original Signal Peptide MILANI ANGGIANI; IS HELIANTI; NIKNIK NURHAYATI; ABINAWANTO ABINAWANTO
Microbiology Indonesia Vol. 11 No. 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

Lipase is one of the most important industrial enzymes, which is widely used in the preparation of food additives, cosmetics, and pharmaceutical industries. In the previous study, we have cloned synthetic Thermomyces lanuginosus lipase gene into Bacillus subtilis and Escherichia coli and resulting low expression for enzyme activity. The aim of this research was to construct the Thermomyces lanuginosus lipase (TLL) gene into Pichia pastoris vector expression with TLL original signal peptide. TLL gene was amplified by PCR and contained original signal peptide and then inserted into pPICZÎ± A between XhoI and XbaI site, and transformed into competent cell E.coli DH5Î±. From the transformant, two of positive recombinants were analyzed by sequencing analysis. As the result,both of two recombinant have a positive target gene which has lipase gene. The correct plasmid was linearized and then was transformed in Pichia pastoris X-33 by electroporation method. Thermomyces lanuginosus synthetic gene lipase has been successfully integrated into chromosome of P. pastoris X-33, which revealed by clear zones arund the colony on Yeast extract Peptone Dextrose Tributyrin (YPD.TB) plate with zeocin. The Thermomyces lanuginosus lipase had an open reading frame of 916bp encoding TLL of 314 amino acids with theoretical molecular mass of 35 kDa. The recombinant enzyme, Thermomyces lanuginosus lipase had optimal temperature at 80ËšC and optimal pH at pH 8.

Co-Authors A. Sofyan A. Sofyan Abdul Basith Achmad Dinoto Achmad Dinoto Achnafani, Dini Ade Sunarma Agung Sudaryono Agus Haryadi Ahmad Aulia Jusuf Alimuddin Andi Salamah ANOM BOWOLAKSONO Arief Boediono ASRI SULFIANTI Bayu , Mariana D. Diki Nurul Huda Djamhuriyah S. Said DODDY IRAWAN SETYO UTOMO Dwiranti, Astari Endang C. Purba Endang Sukara Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini, Eni Erma Primanita Hayuningtyas Fitrianingrum, Nisa Funahashi, Hiroaki H. Julendra Hidayat Hidayat Huriyah Adani Saoemi IS HELIANTI IS HELIANTI is helianti Isma Nur Azzizah Ita M. Nainggolan L. Istiqomah Lina Mulyawati Lulut Dwi Sulistyaningsih M. F. Karimy M. F. Karimy Maria Ulfah Maria Ulfah Mariana D. Bayu Marina Silalahi Marlina Ardiyani Maylasari, Roospita MILANI ANGGIANI Nataniel, Jocelyn NIKNIK NURHAYATI NIKNIK NURHAYATI Nisyawati Nova Anita Noviyanti, Rintis Nurfitriani, Risya Odang Carman Prakoso, Nurul Muhammad PUSPITA LISDIYANTI Putra Mahanaim Tampubolon Rabbil Pratama Aji Retno Lestari Retno Lestari Retno Lestari Retno Lestari Riska S. Wahyuningtyas Rita Rostika RONI RIDWAN Sabar Pambudi Sabbathini, Gabriela Christy Sanya Khaerunnisa Sawung Cindelaras, Sawung SHANNI FERNANDA Shanti Ratnakomala Siti Z Musthofa Solly Aryza Sulistiani ., Sulistiani Sulistiani Sulistiani Sulistiani Sulistiani, Sulistiani Sunarma , Ade Sundari, Ayu Mulia Taniawati Supali Trianti, Lely Uswatun Hasanah UTAMA ANDI Valerry Athalia Priyanka Wibowo Mangunwardoyo WIBOWO MANGUNWARDOYO Wulandari, Sri Rezeki Wulandhary, Sendy Yasman Yasman Yasman Yushinta Fujaya

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