Article Per Year (5 Year)
p-Index From 2021 - 2026
1.203
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan VALENSI Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Warta IHP (Warta Industri Hasil Pertanian) Jurnal Penelitian Karet Indonesian Journal of Chemistry Indonesian Journal of Cancer Teknologi dan Industri Pertanian Indonesia
SUMINAR SETIATI ACHMADI
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

Published : 20 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 20 Documents
Search

 1   2 
Significance of Glucose Addition on Chitosan-Glycerophosphate Hydrogel Properties Dian Susanthy; Purwantiningsih Sugita; Suminar Setiati Achmadi
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.767 KB) | DOI: 10.22146/ijc.21179

Abstract

Chitosan-glycerophosphate hydrogel can be used as dental scaffold due to its thermosensitivity, gelation performance at body temperature, suitable acidity for body condition, biocompatibility, and ability to provide good environment for cell proliferation and differentiation. Previous study showed that glucose addition to the chitosan solution before steam sterilization improved its hydrogel mechanical strength. However, the effectiveness of glucose addition was still doubted because glucose might undergo Maillard reaction in that particular condition. The aims of this study are to confirm whether the glucose addition can increase the hydrogel mechanical strength and gelation rate effectively and also to compare their performance to be dental scaffold. This research was performed through several steps, namely preparation of chitosan-glycerophosphate solution, addition of glucose, gelation time test, gel mechanical strength measurement, functional group analysis, and physical properties measurements (pH, viscosity, and pore size). The result showed that glucose addition did not improve the hydrogel mechanical strength and gelation rate, neither when it was added before nor after steam sterilization. Glucose addition before steam sterilization seemed to trigger Maillard reaction or browning effect, while glucose addition after steam sterilization increased the amount of free water molecules in the hydrogel. Chitosan and glycerophosphate interact physically, but interaction between chitosan and glucose seems to occur chemically and followed by the formation of free water molecules. Glucose addition decreases the solution viscosity and hydrogel pore size so the hydrogel performance as dental scaffold is lowered.
Transformation of Eugenol and Safrole into Hydroxychavicol Budi Arifin; Dumas Flis Tang; Suminar Setiati Achmadi
Indonesian Journal of Chemistry Vol 15, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.955 KB) | DOI: 10.22146/ijc.21227

Abstract

Hydroxychavicol is found in betel leaf at low concentration and is reported to have antibacterial, anti-inflammatory, antioxidant, anticancer, and antimutagenic activities. This study aimed to synthesize hydroxychavicol from eugenol and safrole. Isolation of eugenol from clove oil by alkaline extraction method gave 71% yield, while the isolation of safrole from lawang oil by alkaline extraction method, followed by purification using preparative thin layer chromatography, gave 7% yield. Eugenol demethylation and safrole demethylenation with AlCl3 reagent were successfully produced hydroxychavicol. The yields were 28% and 24%, respectively. Mechanisms of the synthesis are proposed in this article.
Phenolics Profiling and Free Radical Scavenging Activity of Annona muricata, Gynura procumbens, and Typhonium flagelliforme Leaves Extract Dewi Anggraini Septaningsih; Amalia Yunita; Cecep Abdurohman Putra; Irma Herawati Suparto; Suminar Setiati Achmadi; Rudi Heryanto; Mohamad Rafi
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.62124

Abstract

The leaves of Annona muricata (sirsak), Gynura procumbens (sambung nyawa), and Typhonium flagelliforme (keladi tikus) have been used as traditional medicines in Indonesia. This study aims to determine the antioxidant capacity and putatively identified phenolics from the leaves of three medicinal plants forementioned above. We used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method for measuring radical scavenging (antioxidant assay) while the phenolics profiling was determined using UHPLC-Q-Orbitrap HRMS. The results showed that the percentage of radical scavenging activity of G. procumbens leaves extract in ethanol was higher than the other two plants. Phenolics profiling of the three medicinal plants was identified with 38 compounds belonging to flavones and flavanols hydroxycinnamic acid, and several other groups. The number of metabolites identified putatively was 12, 31, and 19 metabolites in the extracts of A. muricata, G. procumbens, and T. flagelliforme, respectively. The results confirmed the correlation between the phenolics presence and the antioxidant capacity of three plants used in this study.
The Potential of 9,10-Anthraquinone in Inhibiting Human Cancer Cells Growth Irmanida Batubara; Arif Rakhman Hakim; Silmi Mariya; Suminar Setiati Achmadi; Valentina Sokoastri; Agustin Sri Mulyatni; Rohayati Suprihatini
Indonesian Journal of Cancer Vol 15, No 1 (2021): March
Publisher : National Cancer Center - Dharmais Cancer Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.903 KB) | DOI: 10.33371/ijoc.v15i1.780

Abstract

Background: 9,10-Anthraquinone (9,10-AQ) is a contaminant on some agricultural products and considered as carcinogenic based on EU Regulation No. 1146/2014. Except for little evidence on experimental rats, there is no strong proof regarding the carcinogenicity in humans. Therefore, it is essential to find a safe dose of this compound since the difference in 9,10-AQ levels will affect cancer cell growth. This research aims to find the 9,10-AQ concentration that does not proliferate the human cancer cells under in vitro study.Methods: In determining the 9,10-AQ concentration that does not proliferate the cancer cells growth, 0.01 to 500 mg/L 9,10-AQ was directly tested on four human cancer cells (colorectal carcinoma HCT 116, colon adenocarcinoma WiDr, breast cancer MCF-7, and cervical cancer HeLa), and the viability of the cells was counted via (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. In the gene expression level, the effects on a selected cancer cell line were determined by qRT-PCR against BAX, BCL-2, PCNA, and P53.Results: The result indicates that 9,10-AQ up to 500 mg/L concentration does not proliferate the cell’s growth but instead inhibits those four cancer cells’ growths. The concentration of 9,10-AQ that inhibits 50% the cancer cells growth (IC50) value was 321.8 mg/L (1.55 mM) against HCT 116 and above 500 mg/L (above 2.40 mM) against WiDr, MCF-7, and HeLa. The 9,10-AQ at 500 mg/L (or 2.40 mM) increases BAX expression and acts as an apoptotic agent on HeLa cells.Conclusions: The investigation has shown that 9,10-AQ up to 500 mg/L concentration does not proliferate the cancer cell growth; instead, it inhibits the HCT 116 and HeLa cells growth. We have preliminary evidence regarding the apoptotic mechanism of 9,10-AQ by increasing BAX gene expression on HeLa cells.
Tyrosinase-Based Paper Biosensor for Phenolics Measurement Fretty Yurike; Dyah Iswantini; Henny Purwaningsih; Suminar Setiati Achmadi
Indonesian Journal of Chemistry Vol 22, No 5 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.72607

Abstract

Environmental pollution resulting from various industrial activities is still a problem for developing countries. The high content of phenolics such as phenols, polyphenols, bisphenol A, catechol, m- and p-cresol from industrial activities are discharged into surface water, soil, and air. Periodic monitoring of the impact of these toxic pollutants is needed for proper control and handling. These detrimental chemicals are usually measured using conventional methods with many drawbacks such as expensive analysis costs, long measurement times, requiring competent analysts, and complicated instrument maintenance. However, the presence of tyrosinase-based paper biosensors is now considered the most promising tool in overcoming the challenges mentioned earlier because they can detect these components quickly, precisely, accurately, inexpensively, and can be measured in situ. The working principle of this biosensor sees optical changes such as dyes, redox processes, and physicochemical properties (aggregation or dispersion) due to the presence of analytes accompanied by the occurrence of color changes that appear. This biosensor uses a layer-by-layer electrostatic method, which causes the deposition of multi-layered films on solid surfaces. In this paper, we review the development of the tyrosinase-based paper biosensor method for phenolic measurement in water, air, and food that gives better results than the conventional methods.
Phytochemical Profiling of Gynura procumbens (Lour.) Merr. Leaves and Stem Extracts Using UHPLC-Q-Orbitrap HRMS Dewi Anggraini Septaningsih; Cecep Abdurohman Putra; Irma Herawati Suparto; Suminar Setiati Achmadi; Rudi Heryanto; Mohamad Rafi
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.74236

Abstract

In Indonesia, Gynura procumbens (Lour.) Merr., known as Longevity Spinach or Sambung Nyawa, is commonly grown in tropical and subtropical Asian countries. Many biological activities of G. procumbens have been reported. As we know, the composition and concentration of metabolites, as well as plant parts will significantly affect the biological activities. In this work, UHPLC-Q-Orbitrap-HRMS was used for the putative identification of metabolites present in 70% ethanol extract of G. procumbens leaves and stem extract. Also, we performed clustering of G. procumbens leaves and stem extracts using principal component analysis (PCA) with the peak area of the identified metabolites as the variable. Thirty-one metabolites were identified, and the number of identified peaks in the leaves is higher than in the stem. Those identified metabolites are phenolics, fatty acids, oxo monocarboxylic acids, porphyrins, and chlorophyll fragments. The PCA results showed that the leaves and stem extracts could be grouped, indicating that the composition and concentration of detected compounds differed.
Produksi Asam Laktat oleh Lactobacillus delbrueckii subsp. bulgaricus dengan Sumber Karbon Tetes Tebu Laita Nurjannah; Suryani Suryani; Suminar Setiati Achmadi; Azmi Azhari
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 9, No 1 (2017): Vol.(9) No.1, April 2017
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (964.784 KB) | DOI: 10.17969/jtipi.v9i1.5903

Abstract

Senyawa asam laktat sangat dibutuhkan di dunia industri. Namun produksi dengan menggunakan mikrob masih menggunakan bahan pangan sebagai substratnya. Alternatif substrat untuk produksi asam laktat  sebagai pengganti penggunaan bahan pangan  sangat diperlukan industri. Tetes tebu merupakan salah satu substrat yang kaya akan sumber  karbon yang dapat digunakan sebagai komponen media pertumbuhan bakteri. Ketersediaannya melimpah dan harganya murah. Tujuan penelitian ini adalah tetes tebu dapat digunakan sebagai alternatif  sumber karbon bakteri Lactobacillus delbrueckii subsp. bulgaricus untuk menghasilkan asam laktat. Langkah penelitian ini meliputi hidrolisis dan detoksifikasi tetes tebu, uji kualitatif gula pereduksi tetes tebu, analisis gula total dengan metode fenol sulfat, penentuan kurva pertumbuhan bakteri, produksi dan ekstraksi asam laktat, serta analisis kualitatif asam laktat dengan menggunakan kromatografi cair kinerja tinggi. Hasil penelitian menunjukkan bahwa tetes tebu dapat digunakan sebagai alternatif sumber karbon. Hal ini terbukti bakteri dapat tumbuh dengan baik ketika media diberi 0.5% tetes tebu. Konsentrasi gula total tetes tebu adalah 1090 g/L. Uji gula pereduksi menunjukkan hasil yang positif untuk uji Selliwanof, uji Benedict, dan uji Barfoed. Pertumbuhan optimum L. delbrueckii subsp. bulgaricus terjadi pada suhu 42°C dengan agitasi 150 rpm. Produksi asam laktat dilakukan selama 24 jam. Kadar asam laktat yang dihasilkan sebesar 2.80% dengan biomassa sel kering sebesar 0.002 g/L dan pH media fermentasi sebesar 4.0. Hasil analisis kualitatif kromatografi cair kinerja tinggi juga menunjukkan bahwa produk dari hasil fermentasi adalah asam laktat.Abstract. Lactic acid is needed as an industrial feed. However, by using a microbial production still uses food material as a substrate. Alternative substrates for the production of lactic acid is needed in industry. Molasses are potential substrates due to the richness in carbon. Molasses also widely available and low-cost material. The objective of the research is molasses can be used as a carbon source needed by Lactobacillus delbrueckii subsp. bulgaricus to produce lactic acid. This study consisted of  hydrolysis and detoxification of molasses, analysis qualitative test of reducing sugar from molasses, analysis of total sugar by phenol sulfuric acid, determination of bacterial growth, production and extraction of lactic acid, and analysis of lactic acid using high performance liquid chromatography. The results showed that molasses can be used as an alternative carbon source as indicated by growth of  bacteria when the media were given 0.5% molasses. Concentration of total sugar molasses was 1090 g/L. The reducing sugar test showed positive results for the Selliwanoff, Benedict, and Barfoed tests. The optimum of L. delbrueckii subsp. bulgaricus growth was at temperature of 42° C and 150 rpm of agitation. Production of lactic acid was conducted in 24 hours. The result of lactic acid from the production was 2.80%. The dry cell biomass was 0.002 g/ L at pH of  fermentation media was 4.0. Analysis HPLC also showed that lactic acid was the product of fermentation.
Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics Rut Novalia Rahmawati Sianipar; Komar Sutriah; Dyah Iswantini; Trivadila; Suminar Setiati Achmadi
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.1-20

Abstract

The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future.
In Silico Screening Anticancer of Six Triterpenoids toward miR-494 and TNF-α Targets Vikra Ardiansyah Zaini; Purwantiningsih Sugita; Luthfan Irfana; Suminar Setiati Achmadi
Jurnal Kimia Sains dan Aplikasi Vol 23, No 4 (2020): Volume 23 Issue 4 Year 2020
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3049.44 KB) | DOI: 10.14710/jksa.23.4.117-123

Abstract

Hepatocellular carcinoma (HCC) accounts for up to 90% of all primary liver cancers worldwide. Cinobufagin is recognized to inhibit miR-494 as the HCC target. Increased expression of TNF-α results in an inadequate response to liver anticancer drugs. The models in this study were cinobufagin, cycloartenol, and ethyl acetate fractions of Ganoderma lucidum, 2–5. Seven docking targets in this study were Akt, ERK1, ERK2, PI3K, TNF-α, TNFR1, and TNFR2. Cycloartenol and compound 4 comply with Veber’s rules, Lipinski’s rule of 5, and demonstrate moderate toxicity. The action implies a potential docking target since it produces bond affinities with the compound 2–5 that agree with the IC50 in the literature, which is based on in vitro experiments. Akt as a receptor target is AZD5363. Cycloartenol shows a low ability to inhibit Akt. Conversely, compound 4 inhibits the Akt better than that of cycloartenol, although it is not as good as cinobufagin and AZD5363. Therefore, compound 4, a triterpenoid with a basic framework of lanostane has the potential to be an anticancer candidate for the liver.
Tea Constituent in Protecting Glyphosate Effect on Human Breast Cancers Cells (MCF-7) Growth Batubara, Irmanida; Suprihatini, Rohayati; Mariya, Silmi; Achmadi, Suminar Setiati; Sokoastri, Valentina; Mulyatni, Agustin Sri; Hakim, Arif Rakhman
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.33229

Abstract

Glyphosate, which has been documented as a contaminant in tea, promotes the proliferation of human breast cancer cells (MCF-7). Tea, on the other hand, includes various antioxidants, including epigallocatechin gallate (EGCG), which may protect against cancer cell proliferation. The purpose of this research is to determine the preventive effect of concentrated brewed green tea on MCF-7 development caused by glyphosate. The glyphosate concentration that promotes MCF-7 development was determined using a serial concentration of glyphosate. Glyphosate concentrations of up to 64 mg/L were shown to have no effect on MCF-7 cell proliferation. Concentrated brewed tea and EGCG 200 mg/L have the potential to suppress MCF-7 cell proliferation in the presence of glyphosate up to 512 mg/L. The combination of glyphosate and concentrated brewed tea or EGCG protects against glyphosate toxicity via altering the expression of tumor suppressor protein (p53).
Co-Authors -, Rienoviar . TRIVADILA ADE NOOR SYAMSUDIN Adi Cifriadi Adi Cifriadi, Adi Agustin Sri Mulyatni Amalia Yunita Anjas Asmara Samsudin Anuraga Jayanegara Arif Rakhman Hakim Arif Rakhman Hakim Atep Dian Supardan Azmi Azhari Budi Arifin C Hanny Wijaya Cecep Abdurohman Putra Dian Susanthy Dumas Flis Tang Dyah Iswantini Erika B. Laconi Fretty Yurike Harsi D. Kusumaningrum Henny Purwaningsih Ihsan Anggara Irma Herawati Suparto Irmanida Batubara Komar Sutriah Laita Nurjannah Luthfan Irfana Mohamad Rafi Mohamad Rafi Muhana Nurul Hidayah Muhana Nurul Hidayah, Muhana Nurul Mulyatni, Agustin Sri Pristian Yuliana Purwantiningsih Sugita Purwantiningsih Sugita Raffi Paramawati Rienoviar - ROHAYATI SUPRIHATINI Rohayati Suprihatini Rudi Heryanto Rut Novalia Rahmawati Sianipar Septaningsih, Dewi Anggraini SILMI MARIYA Silmi Mariya Suliantari Suliantari Suryani Suryani TEDJA -IMAS Tun Tedja Irawadi Valentina Sokoastri Valentina Sokoastri Vikra Ardiansyah Zaini Yuyu Yundhana