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Mahbubillah, M. Ainul
Universitas Muhammadiyah Lamongan
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Diesel Fuel Degradation Activity of Hydrocarbon-Degrading Bacteria from Nusantara Fisheries Port Brondong Lamongan Mahbubillah, M. Ainul; Tamam, Muhammad Badrut; Ayuni, Rieke Dwi; Savitri, Nynda Ayu Nadira; Ramadani, Aisyah Hadi
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10887

Abstract

The Nusantara Fisheries Port (Pelabuhan Perikanan Nusantara - PPN) in Brondong, Lamongan Regency, is a port with high ship activities. This has led to hydrocarbon pollution in the form of diesel fuel and oil in the waters of PPN Brondong. The initial effort to address this pollution is by isolating and characterizing hydrocarbon-degrading bacteria present in these waters. This research is a strategic step in managing petroleum hydrocarbon spills in the area through bioremediation processes, either via bioaugmentation or biostimulation for further study. The research method involves isolating hydrocarbon-degrading bacteria from the waters of PPN Brondong using the spread plate isolation method. Different colony characteristics are selected for the purification of bacterial isolates. The obtained bacterial isolates are then subjected to hydrocarbon degradation activity by culturing them in a minimal medium containing diesel fuel. The degradation activity is measured using total petroleum hydrocarbon (TPH) testing. The results of this study yielded 7 different isolates from the fuel oil-contaminated sites in PPN Brondong. Culturing in a minimal medium containing diesel fuel yielded results showing that all isolates possess the ability to degrade hydrocarbons, as evidenced by the reduction in TPH.
Banana (Musa × Paradisiaca) Corm as an Alternative Substrate for Nata by Acetobacter xylinum M. Ainul Mahbubillah; Fatma Atiq Khoridah; Muhammad Badrut Tamam; Fatan Mujahid
Food ScienTech Journal Vol 7, No 2 (2025)
Publisher : University of Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/fsj.v7i2.33847

Abstract

Banana corms, an underutilized by-product of banana (Musa × paradisiaca) cultivation, were investigated as a novel substrate for nata production using Acetobacter xylinum fermentation. This study evaluated the effects of varying sucrose concentrations (5%, 10%, and 15%), ammonium sulfate levels (0.5%, 1%, and 1.5%), and pH (3.5, 4, and 4.5) on nata thickness, wet weight, dry weight, water holding capacity (WHC), and organoleptic properties. The highest nata thickness (6.44 mm) and wet weight were observed in S3, A3, P3 (15% sucrose, 1.5% ammonium sulfate, pH 4.5). The highest dry weight was also obtained under these conditions. WHC varied between 6.50% and 17.00%, with S3, A2, P2 (15.00%) and S2, A3, P1 (16.00%) showing the highest values. Organoleptic analysis indicated that banana corm nata had a murky colour, slightly chewy texture, and sour taste, comparable to nata de coco but differing in acidity. The findings confirm that banana corms are a viable substrate for nata production, providing an innovative and sustainable approach to agricultural waste utilization with potential industrial applications.
Diesel Fuel Degradation Activity of Hydrocarbon-Degrading Bacteria from Nusantara Fisheries Port Brondong Lamongan M. Ainul Mahbubillah; Muhammad Badrut Tamam; Rieke Dwi Ayuni; Nynda Ayu Nadira Savitri; Aisyah Hadi Ramadani
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10887

Abstract

The Nusantara Fisheries Port (Pelabuhan Perikanan Nusantara - PPN) in Brondong, Lamongan Regency, is a port with high ship activities. This has led to hydrocarbon pollution in the form of diesel fuel and oil in the waters of PPN Brondong. The initial effort to address this pollution is by isolating and characterizing hydrocarbon-degrading bacteria present in these waters. This research is a strategic step in managing petroleum hydrocarbon spills in the area through bioremediation processes, either via bioaugmentation or biostimulation for further study. The research method involves isolating hydrocarbon-degrading bacteria from the waters of PPN Brondong using the spread plate isolation method. Different colony characteristics are selected for the purification of bacterial isolates. The obtained bacterial isolates are then subjected to hydrocarbon degradation activity by culturing them in a minimal medium containing diesel fuel. The degradation activity is measured using total petroleum hydrocarbon (TPH) testing. The results of this study yielded 7 different isolates from the fuel oil-contaminated sites in PPN Brondong. Culturing in a minimal medium containing diesel fuel yielded results showing that all isolates possess the ability to degrade hydrocarbons, as evidenced by the reduction in TPH.
Penerapan Irigasi Tetes Bertenaga Surya dengan Sistem Otomatis Mikrokontroler Pada Kelompok Tani Kabupaten Lamongan Heri Ardiansyah; Muhammad Shodiq; M Ainul Mahbubillah; Rohmatin Agustina; Abdullah Hakim Gymnastiar; Muhammad Rizky Rahmadani; Fatahillah Akrom; Ahmad Faris Rachmad Putra; Hanif Azhar Ramadhan; Zufar Faiil Haq
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 14, No 1 (2025): Mei
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v14i1.93488

Abstract

Keterbatasan sumber daya air di lahan tadah hujan menjadi tantangan utama bagi petani, terutama selama musim kemarau, yang membatasi aktivitas budidaya dan berdampak pada pendapatan rumah tangga. Program pengabdian masyarakat ini bertujuan untuk mengatasi masalah tersebut melalui penerapan teknologi irigasi tetes bertenaga surya dengan sistem kecerdasan buatan menggunakan sensor kelembaban dan suhu tanah di Kelompok Tani Kabupaten Lamongan. Metode pelaksanaan meliputi perancangan dan instalasi sistem irigasi otomatis, pelatihan penggunaan teknologi kepada petani, serta pendampingan budidaya cabai selama musim kemarau. Hasil program menunjukkan bahwa lebih dari 90% petani mengakui manfaat dari irigasi otomatis ini dan berencana untuk menerapkannya, baik secara mandiri (25%) maupun melalui kelompok tani (32,5%). Selain itu, 35% petani memilih untuk mengombinasikan sistem irigasi otomatis dengan metode konvensional untuk menyesuaikan kebutuhan air sesuai musim. Penerapan teknologi ini tidak hanya memungkinkan petani untuk tetap menanam di musim kemarau, tetapi juga meningkatkan pendapatan mereka secara signifikan. Kesimpulannya, teknologi irigasi tetes bertenaga surya yang dilengkapi kecerdasan buatan terbukti efektif dalam meningkatkan efisiensi penggunaan air dan kesejahteraan petani, serta dapat direplikasi untuk mendukung ketahanan pangan dan produktivitas pertanian di wilayah lain.
Pathogenesis-related proteins response in citronella grass (Cymbopogon nardus) infected by Curvularia andropogonis ROFIATUN SOLEKHA; M. AINUL MAHBUBILLAH; M. BADRUT TAMAM; FADHILATUSTSANI SOFIALANA; ALIFIA HAYA AS-SYIFA; ACHMAD DJAZULI; HERY PURNOBASUKI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 11 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261110

Abstract

Abstract. Solekha R, Mahbubillah MA, Tamam MB, Sofialana F, As-Syifa AH, Djazuli A, Purnobasuki H. 2025. Pathogenesis-related proteins response in citronella grass (Cymbopogon nardus) infected by Curvularia andropogonis. Biodiversitas 26: 5485-5494. The development and growth of citronella grass (Cymbopogon nardus) are often hindered by Curvularia andropogonis fungal infection, which causes leaf disease. One key approach is to identify the expression of Pathogenesis-Related (PR)-17 and PKS type III defense proteins in citronella after C. andropogonis infection, serving as a model for counterattacking fungal infection. This study aimed to (i) determine chalcone synthase enzyme activity in C. nardus against C. andropogonis infection, (ii) identify the PR-17 response of C. nardus leaves, and (iii) identify the metabolic variations of flavonoid derivatives of C. nardus leaves. High-Performance Liquid Chromatography was carried out to analyze the enzyme activity. Protein identification was performed using sodium dodecyl sulfate. The variation of secondary metabolites was measured using Gas Chromatography-Mass Spectrometry. The results indicated that the enzyme CHS activity in infected C. nardus leaves had increased significantly compared to healthy leaves. PR-17, PR-2, and PR-3 were only expressed in C. nardus infected with C. andropogonis. Profile of flavonoid-derived metabolites in C. nardus infected with C. andropogonis changed significantly, involving seven flavonoid-derived metabolites. The activation of CHS, accompanied by elevated expression of defense-related proteins and increased levels of flavonoid derivatives, creates a synergistic defense strategy in infected C. nardus. These responses strengthen the plant immunity, restrict fungal colonization, and enhance overall resistance, making them important indicators of plant-pathogen interactions.
Co-Authors Abdul Rozaq Alfarisi Abdullah Hakim Gymnastiar ACHMAD DJAZULI Agustina, Rohmatin Ahmad Faris Rachmad Putra Aini, Nur Sofiatul Aisyah Hadi Ramadani Aisyah, Neelam ALIFIA HAYA AS-SYIFA Ayuni, Rieke Dwi Dwi Zulianti FADHILATUSTSANI SOFIALANA Fatahillah Akrom Fatan Mujahid Fatma Atiq Khoridah Fika Nur Fitriana Putri Bukhori Hadad Alwi Hanif Azhar Ramadhan Heri Ardiansyah Hery Purnobasuki Khakim, Arsanul Laila Ainur Rohmah M. BADRUT TAMAM Muhammad Badrut Tamam Muhammad Badrut Tamam Muhammad Rizky Rahmadani Muhammad Shodiq Muhammad Shodiq, Muhammad Muhammad Sulton Nynda Ayu Nadira Savitri Prastiyanto, Muhammad Evy Purwanti, Ika Redjeki, Endah Sri Rieke Dwi Ayuni Rofiatun Solekha Savitri, Nynda Ayu Nadira Setiyowati, Putri Ayu Ika Suhariyati Tamam, Muhammad Badrut Yuanita Rachmawati Zufar Faiil Haq