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EKSPRESI KORESEPTOR HUMAN IMMUNODEFICIENCY VIRUS CCR5 DAN CXCR4 PADA SUBSET SEL LIMFOSIT T SERTA MONOSIT Agnes Rengga Indrati; Hinta Meijerink; Herry Garna; Bachti Alisjahbana; Ida Parwati; Reinout van Crevel; Andre van der Venn
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1012

Abstract

Chemokine receptors CCR5 and CXCR4 which lied on lymphocyte cell surface play important role in HIV infection and pathogenesis.The expression of these chemokine receptors will affect progressively the disease. The objectives of the study are to find the distributionof lymphocyte T cell subset and monocyte among the peripheral blood mononuclear cells and to know the determination of CCR5 andCXCR4 co receptors expression on T lymphocyte cells subset and monocyte. This study is a preliminary study to explore the distributionof co receptors CCR5 and CXCR4 expression in healthy people. The sample taken is peripheral blood mononuclear cells (PBMC) fromhealthy subjects. The identification of T lymphocyte cells subsets and monocyte, and the expression of CCR5 and CXCR4 co receptorswere determined using flowcytometry. The memory T cell (CD4+CD45RO) is found to be the largest proportion among T lymphocyte cell(66.2%), whereas the other T lymphocyte cell subset, regulatory T cell, which identified by CD25+ high expression was found between2.0-5.3% from the whole T lymphocyte cell. The proportion of CXCR4 co receptors was found higher compare to CCR5 co receptorson all T lymphocyte subsets and monocyte. Only small proportion of monocyte expresses both co receptors (2.85%), but most of the Tlymphocyte cell expressed both CCR5 and CXCR4. The expression of the CXCR4 on regulatory T cell (18.18%) is the lowest compared toother cells, but the fluorescence intensity of both co receptors was very high (CCR5 53.53 and CXCR4 49.33). The different distributionof CCR5 and CXCR4 co receptors among T lymphocyte cell subsets and monocyte will influence the vulnerability and the pathogenicityof HIV infection.
RANCANGAN PRIMER SPESIFIK GEN MACROPHAGE MANNOSE RECEPTOR (MMR) UNTUK POLYMERASE CHAIN REACTION (PCR) DAN SEKUENSING DEOXYRIBO NUCLEIC ACID (DNA) Yani Triyani; Nurizzatun Nafsi; Lelly Yuniarti; Nanan Sekarwana; Endang Sutedja; Dida Ahmad Gurnida; Ida Parwati; Bachti Alisjahbana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1120

Abstract

The order (sequencing) determinationof DeoxyribonucleicAcid (DNA) bases is the gene’s most basic information, using the methodof Polymerase Chain Reaction (PCR) as its stage. A key factor of successful detection by PCR is specific PCR primer design choice. Thedetection of diversity of Mycobacterium Mannose Receptor (MMR) gene, responsible for recognizing mannosylated antigen structureof Mycobacterium tuberculosis (M.tb) by DNA sequencing of exon 7 chromosome 10p12, related to susceptiblity for PulmonaryTuberculosis(TB), was first performed in China in 2012. The purpose of this study was to find specific primerfromboth design originatedfrom the research in China/primer I and my own design/primer IIby using Primer3 software. This study was based on 10 healthy subjectsand was a preliminary study of a research titled. The Relationship of Single Nucleotide Polymorphisms (SNPs) of Macrophage MannoseReceptor Gene to Pulmonary Tuberculosis Cases. The examination materials consist of 3 mL of EDTA blood and DNA extraction from itsbuffy coat. The resulting DNA was processed by PCR to amplify MMR gene with primer I and II. The primer I successfully amplified DNAfragments up to 780bp while primer II only 329 bp. The MMR gene DNA sequencing analysis was performed on the amplification resultof both kinds primers by using DNA Baser and Ensembl−BLAST software. The results were different, DNA sequencing result by using theprimer I was found in several chromosomes and also in several loci. Whereas, by using the primer II, it was only found in chromosome10 and in the same locus. Based on this study, it can be concluded that the specific primer design is one of the most important factorsin the success of DNA sequencing.
Analisis Hubungan Faktor Lingkungan Fisik Terhadap Keberadaan Jumlah Nyamuk Aedes Aegypti di Kota Bandung obin sarwita; Bachti Alisjahbana; Dwi Agustian
The Indonesian Journal of Infectious Diseases Vol 4, No 1 (2018): The Indonesian Journal of Infectious Diseases
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.905 KB) | DOI: 10.32667/ijid.v4i1.45

Abstract

Latar belakang : Penyakit dengue sampai saat ini belum dapat dikendalikan dengan baik. Transmisi virus dengue ke manusia disebabkan oleh interaksi yang kompleks dan dinamis oleh beberapa faktor diantaranya lingkungan fisik, biologi dan sosial. Penelitian ini bertujuan untuk mengetahui hubungan lingkungan fisik dengan keberadaan nyamuk Aedes aegypti penyebab penyakit dengue di Kota Bandung. Metode : Penelitian ini merupakan studi ekologi dangan pendekatan cross sectional. Populasi dalam penelitian ini adalah seluruh bangunan rumah di Kota Bandung. Besar sampel sebanyak 160 rumah yang terpilih dengan multistage random sampling. Data keberadaan jumlah nyamuk diperoleh dari alat perangkap nyamuk yang dipasang di 16 kelurahan. Analisis bivariat menggunakan korelasi rank spearman, uji man whitney dan kruskal walis. Analisis multivariate menggunakan regresi zero inflated poisson. Hasil : Luas ventilasi, kepadatan hunian, jumlah baju menggantung, kelembaban udara dan suhu udara tidak berkorelasi dengan jumlah nyamuk Aedes aegypti, sedangkan penampungan air positif jentik positif jentik memiliki korelasi positif dan signifikan terhadap jumlah nyamuk Aedes aegypti (p=0.017). Hasil analisis menggunakan regresi Zero Inflated Poisson, ,tidak ada variabel yang signifikan dengan nilai p>0,05 dan nilai AIC=360. Kesimpulan: Ada korelasi positif dan signifikan antara tempat penampungan air positif jentik dengan jumlah nyamuk Aedes aegypti. Analisis dengan model zero inflated poisson tepat digunakan pada penelitian ekologi dimana observasi dengan nilai nol lebih banyak ditemukan. 
Faktor Intrinsik yang Berhubungan dengan Keberhasilan Pengobatan Tb Paru marni banowati; Ida Parwati; Hadyana Sukandar; Rovina Ruslami; Bachti Alisjahbana; Kumia Wahyudi
The Indonesian Journal of Infectious Diseases Vol 4, No 2 (2018): The Indonesian Journal of Infectious Diseases
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.467 KB) | DOI: 10.32667/ijid.v4i2.48

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Latar belakang: Tuberkulosis (TB) merupakan penyakit infeksi yang disebabkan oleh Mycobacterium tuberculosis yang telah menginfeksi hampir sepertiga penduduk dunia. Indikator yang digunakan sebagai evaluasi pengobatan yaitu angka keberhasilan pengobatan. Tujuan dari penelitian ini adalah untuk mengevaluasi hasil pengobatan pada pasien baru TB Paru BTA positif dan faktor yang berhubungan dengan keberhasilan pengobatan TB Paru di Puskesmas Kota Bandung. Metode: Penelitian analitik-kuantitatif dengan rancangan cross sectional prospektif. Sampel dalam penelitian ini pasien baru TB paru BTA positif pada triwulan ke empat yaitu bulan Oktober, November dan Desember 2016 dari sepuluh Puskesmas di Kota Bandung yaitu UPT. Puskesmas Puter, Garuda, Ujungberung Indah,  Sukajadi, Kopo, Ibrahim Adji, Arcamanik, Mohammad Ramdan, Salam, dan Antapani. Besar sampel 77 responden yang diambil dengan teknik multistage random sampling. Analisa bivariabel dengan uji chi square. Hasil : Angka keberhasilan pengobatan TB pada fase intensif 64% (IK 95%: 73,2%-89,9%); sedangkan pada fase lanjutan sebesar 94,8% (IK 95%: 87,4%-98,0%). Berdasarkan analisis bivariabel yang berhubungan dengan keberhasilan pengobatan TB paru  adalah jumlah BTA pada pengamatan bulan ke-2 (p: 0,05) dan status HIV pada pengamatan bulan ke-5 (p: 0,007). Kesimpulan: jumlah BTA dan status HIV berhubungan dengan keberhasilan pengobatan TB Paru di Puskesmas Kota Bandung.
POLA DAN KEPEKAAN KUMAN BIAKAN SPUTUM SERTA KARAKTERISTIK PASIEN PNEUMONIA DI RSUP. DR. HASAN SADIKIN BANDUNG Menik Herdwiyanti; Bachti Alisjahbana; Prayudi Santoso
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 7, No 1 (2021): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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Abstract

ABSTRAKLATAR BELAKANG, Pneumonia merupakan penyebab kematian dan angka perawatan yang tinggi, baik di dunia maupun di Indonesia. Pemeriksaan gram dan biakan sputum merupakan pemeriksaan yang sederhana, mudah dan tidak invasif pada diagnosis pneumonia. Pada pedoman IDSA/ATS ditemukan kuman yang berbeda dengan penelitian EPIC II di Asia pada tahun 2007. Efikasi antibiotik dipegaruhi oleh tingkat resistensi kuman yang semakin meningkat dan perubahan pola kuman multiresisten. Pola kuman dan kepekaan serta karakteristik pasien pneumonia di institusi lokal perlu diketahui agar dapat memberikan rekomendasi terapi empiris yang lebih sesuai dengan kuman penyebab. METODE, penelitian deskriptif terhadap pasien pneumonia berusia lebih dari 14 tahun , tanpa intubasi dan/atau penggunaan ventilator, yang telah dikonsultasikan ke divisi Respirologi dan penyakit kritis, dan dirawat di SMF Ilmu Penyakit Dalam RSUP Dr. Hasan Sadikin Bandung sejak 1 April 2016 sampai dengan 30 Juni 2016. HASIL, 87 pasien dengan pneumonia memberikan hasil biakan sputum dengan pola kuman yang cukup berbeda dengan pedoman IDSA/ATS, terutama pola kuman penyebab CAP. Kuman penyebab CAP terbanyak adalah Klebsiella pneumoniae (28,9%), Penyebab HCAP terbanyak adalah Acinetobacter baumanii (19%) dan penyebab HAP terbanyak adalah Pseudomonas aeruginosa (23,8%). Bakteri K.pneumoniae pada pasien HCAP resisten terhadap antibiotik golongan sefalosporin, A.baumanii pada CAP dan HAP memiliki resistensi hingga 50% terhadap Sefalosporin generasi ke-3, dan P.aeruginosa banyak ditemukan resisten hingga 40% terhadap sefepim, namun masih sensitif terhadap meropenem. SIMPULAN, Pola kuman yang ditemukan pada penelitian ini berbeda dengan pola yang dipaparkan oleh IDSA/ATS. Pemberian antibiotik definitif disesuaikan dengan hasil biakan sputum dan kepekaannya, namun pemberian antibiotik empiris harus disesuaikan dengan pola kuman dan kepekaan di institusi lokalKata Kunci: Pneumonia, Sputum, Pola Kuman, Resistensi.ABSTRACTINTRODUCTION. Pneumonia causes high mortality and morbidity around the world including Indonesia. Sputum stain and culture is simple, easy and uninvasive way to determine causal pathogen. Guidelines such as IDSA/ATS guideline suggest different patterns of microorganism compare to EPIC II Study in Asia. Antibiotic efficacy were affected by increasing pathogen resistance level and changes in multi-resistance pathogens. The needs of localized pathogen pattern and resistance level, as well pneumonia patients characteristics were to have a proper antibiotics recommendation. METHODS. Descriptive study on over 14 years old pneumonia Patients with no use of intubation or ventilator. Patients were consulted to critical illness and respirology division and admitted to Internal Medicine Ward RSUP Dr. Hasan Sadikin Bandung. Study conducted since 2016, 1st April to 2016, 30th of June. RESULTS. Eighty-Seven patients were positive sputum-culture pneumonia. Patterns of microorganism differ from guidelines, especially CAP Pathogens. Most pathogen for CAP is Klebsiella pneumoniae (28,9%), HCAP caused mostly by Acinetobacter baumanii (19%) and casual for HAP were Pseudomonas aeruginosa (23,8%). HCAP K.pneumoniae resistent to cephalosporin, CAP and HAP A.baumanii had 50% resistancy to 3rd generation cephalosporin and P.aeruginosa resistant to Cefepime up to 40% but sensitive to meropenem. CONCLUSION. Pathogen pattern discovered in this study were differ compared to those in IDSA/ATS Guidelines. Definitive antibiotic therapy must be correspond to pathogen culture and sensitivity results, but Empirical antibiotic must be adjusted to local pathogen and resistance.Kata Kunci: Pneumonia, Sputum, Pola Kuman, Resistensi.Keywords: Three, Upto, Five, Words
Diabetes mellitus patients in Indonesia: management in a tertiary hospital compared to primary health care Hikmat Permana; Raspati Cundarani Koesoemadinata; Nanny Natalia Mulyani Soetedjo; Nury Fitria Dewi; Novi Jayanti; Sofia Imaculata; Rovina Ruslami; Bachti Alisjahbana; Susan Margaret McAllister
Universa Medicina Vol. 41 No. 2 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.157-168

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Background The increasing prevalence of diabetes mellitus (DM) requires that patients have greater access to care, which is yet lacking in many low- and middle-income countries and the quality of which varies between health care facilities. We compare the characteristics, complications, and risk profile of diabetes in patients receiving care in primary and tertiary level health facilities in Bandung, Indonesia. MethodsAdult DM patients were recruited from 25 community health centres (CHCs) and the outpatient clinic at one referral hospital. Key data collected and compared to national guidelines were DM history, treatment, complications, blood pressure, height, weight, and laboratory examinations on glycated haemoglobin (HbA1c), lipid profile, and creatinine. Data analysis was by chi-square test. ResultsOf the 809 DM patients (median age 59 years, 63% female, 98% type 2 DM), 318 (39%) were from CHCs and 491 (61%) from the hospital. Overall median HbA1c was 8.3%, with no difference between CHC and hospital patients. Only 32% of patients with HbA1c ≥10% were on insulin (CHCs 5.9%, hospital 42.9%), and only 18% of those on insulin had glycaemic control. Hypertension was common (CHCs 62%, hospital 51%, p<0.001), and only 44% of CHC and 34% of hospital patients received antihypertensive therapy. Among those with macrovascular complications, only 32% (CHCs) and 26% (hospital) were receiving aspirin. The numbers reaching the treatment targets were low for those on antihypertensives and lipid-lowering medications (80/251 and 11/105, respectively). ConclusionGlycaemic control and management of complications of DM patients at both health care levels need considerable improvement.
Early development of self‐administered COVID‐19 rapid test based on nucleocapsid detection in saliva sample Siti Soidah; Toto Subroto; Sari Syahruni; Fauzian Giansyah; Henry Chandra; Dhiya Salsabila; Bachti Alisjahbana; Nisa Fauziah; Hesti Lina Wiraswati; Leonardus Wiydatmoko; Basti Andriyoko; Anita Yuwita; Muhammad Yusuf
Indonesian Journal of Biotechnology Vol 27, No 3 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.72269

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More than 6,000,000 people have died due to the coronavirus (COVID‐19) pandemic. This disease spread quickly due to its highly contagious nature. The SARS‐CoV‐2 virus that causes the disease can be transmitted through saliva droplets secreted by infected people at a distance of less than 1 m. As a result, saliva has been accepted as an alternative specimen for COVID‐19 detection by the Centers for Disease Control and Prevention (CDC). Furthermore, WHO recommended the use of rapid antigen tests based on lateral flow immunoassay when reverse transcription‐polymerase chain reaction (RT‐PCR) is not available. We developed a saliva‐based rapid antigen test by optimizing the antibody concentration and optimum pH for the conjugation of antibody and gold nanoparticles. We found that the best running buffer formulation consisted of 75 mM sodium phosphate buffer, 1% NaCl, 1% Triton X‐100, 0.5% N‐acetyl‐L‐cysteine, and 0.02% sodium azide. The addition of a mucolytic agent in the buffer can reduce the viscosity of saliva, thus improving sensitivity. The rapid test developed detected the lowest concentration of nucleocapsid protein at 0.1 μg/mL. Our study revealed 100% specificity against negative COVID‐19 saliva and no cross‐reaction with avian influenza virus hemagglutinin.
Protective Effect of the T1212C Macrophage Mannose Receptor Gene Polymorphism on Pulmonary Tuberculosis Yani Triyani; Julia Hartati; Budiman Budiman; Ida Parwati; Bachti Alisjahbana
Global Medical & Health Communication (GMHC) Vol 10, No 3 (2022)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v10i3.10706

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The interaction between the mannose receptor, which is encoded by the macrophage mannose receptor (MMR) gene, and the most virulent antigen (the mannose-capped lipoarabinomannan) cell wall of virulent strains of Mycobacterium tuberculosis trigger an innate and adaptive immune response. It also produces pro and anti-inflammatory cytokines, which play a role in the pathogenesis of tuberculosis (TB) infection. Therefore, MMR gene polymorphism is a risk factor associated with the prognosis for active pulmonary TB. This study aimed to determine the correlation between MMR gene polymorphism and active or latent pulmonary tuberculosis. In this phase, MMR gene polymorphism was analyzed using a case-control design consisting of 74 control group subjects (patients with latent TB) and 74 case groups (patients with active pulmonary TB). The subject’s MMR gene DNA sequencing examination. The study was conducted at the Teaching Hospital, Faculty of Medicine, Universitas Padjadjaran Bandung, from February 2014 to January 2015. The statistical analysis used chi-square and odds ratio. The study’s result has shown the MMR gene polymorphism factor that correlated to the incidence of active pulmonary TB was T1212C (OR=0.253; 95% CI=0.111−0.575; p=0.001). There was an MMR gene in one SNP in the control group (C1323T) only and five single nucleotide polymorphisms (SNPs) in both groups (C1303T, C1221, T1212C, G1186A, and G1195A). Therefore, it can be concluded that MMR gene polymorphism on the T1212C site correlated with the incidence of active pulmonary tuberculosis and was protective.
Application of Gold Nanoparticles Synthesized with Malva Nut Gum as Reducing Agent for scFv Conjugates in a Colorimetric Chikungunya Virus Detection Muhamad Zulchoir Rasyid; Ratna Yulistya Ningrum; Sinta Setyaningrum; Budi Santoso; Muhammad Yusuf; Bachti Alisjahbana; Toto Subroto; Bevi Lidya
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4493

Abstract

Gold nanoparticles have good surface plasmon properties in the presence of free electrons on their surface. The presence of free electrons on the surface of the plasmon causes gold nanoparticles to be able to bind to various kinds of biomolecules and proteins, including single-chain variable fragments (scFv). In this study, gold nanoparticle conjugates with Chikungunya antiviral scFv have been successfully prepared with the aim of developing a colorimetric method for rapid detection of envelope protein Chikungunya virus (E2 CHIKV). The manufacture of gold nanoparticles was carried out using malva nut gum and trisodium citrate as reducing agents. The results showed the formation of the AuNP-scFv conjugate which was characterized by a shift in the maximum wavelength for gold nanoparticles synthesized using malva nut gum as a reducing agent from 532.89 nm to 536.62 nm. Similarly, for gold nanoparticles synthesized using trisodium citrate as a reducing agent, the maximum wavelength shifted from 528.99 nm to 531.95 nm. The detection result of E2 CHIKV protein using the AuNP-scFv conjugate has not shown a positive result
Cause of The Low Positivity of Blood Culture in Septic Patients Yana, Krishna; Alisjahbana, Bachti; Hartantri, Yovita
Jurnal Penyakit Dalam Indonesia Vol. 5, No. 4
Publisher : UI Scholars Hub

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Abstract

Introduction. Sepsis due to bacterial infection is one of the main causes of high disease morbidity and mortality. Management of sepsis, the detection of bacteriemia by examination of blood cultures has an important role, but until now the positivity of blood culture results at Hasan Sadikin Hospital (RSHS) is still low (13.45%). The purpose of this study was to determine profile the causes of low positivity of blood culture results in sepsis patients in RSHS. Methods. A cross sectional study was conducted on sepsis patient treated in Emergency Un it (ER) and inpatient of Hasan Sadikin hospital Internal Medicine from December 2016 until April 2017. This study collects patient clinical information, laboratory, history Administration of antibiotics, and techniques of examination of bacterial blood cultures in sepsis patients. Results. During the study period 179 subjects were obtained. The subject mainly uses the Health Guarantee Agency (BPJS) for financing. The positivity of blood cultures was found to be 24%. The mean leukocyte count on positive blood culture results was 18,100 / mm3 and there were 79.07% cultures with Neutrofil Limfosit Count Ratio (NLCR) >10. The time for taking blood cultures is mainly done at> 6 hours after admission. The number of blood cultures is taken at least once. The amount of blood volume in each culture tube is mostly 5 cc. Taking blood cultures is mainly carried out by competent nurses who have not received blood culture taking training. Conclusions. The positive results of blood culture in septic patients in RSHS are still low. Taking blood cultures should be carried out in accordance with the guidelines, which consist of not giving antibiotics before taking blood culture, taking blood time at admission, the number of blood taken for culture >1 time, the amount of blood volume in a culture tube is at least 10 cc, use 2 types of antiseptics, the use of antiseptics on culture bottle caps, the time lag between antiseptic action and blood collection for culture. It is necessary to monitor the factors that influence the positivity of blood cultures in septic patients. Researchers also recommend training and policy improvements to improve blood culture positivity.
Co-Authors Afandi, Ressa Novita Agnes Rengga Indrati Ahmad Sulaeman Amaylia Oehadian Andre van der Venn Angèle JGMGerver-Jansen Angky Tririni Anita Yuwita Anna Alisjahbana Annisa Ikhsanawati, Annisa Ardini S. Raksanagara Ardini S. Raksanagara, Ardini S. Baskoro, Toni Basti Andriyoko Bestari, Muhamad Begawan Bevi Lidya Bevi Lidya Budi Santoso Budiman , Budiman Clara M Kusharto Depamede, Brian Umbu Rezi Dewi Kartika Turbawaty Dhiya Salsabila Dick van Soolingen Dida Ahmad Gurnida Dwi Agustian Dwihardiani, Bintari Edhyana Sahiratmadja Endang Sutedja Eva Mardiana Hidayat Evan Susandi Fauzian Giansyah Febriani, Ulfah Dwi Felisia . Finny Fitry Yani Gumilang, Manik Intan Guntur Darmawan Hadyana Sukandar Hafidhoh, Sanny Hendra Subroto, Hendra Henriette A Delemarre–van de Waal Henry Chandra Herry Garna Hesti Lina Wiraswati Hidayat, Eva Mardiana Hikmat Permana Hikmat Permana Hinta Meijerink Hofiyah Djauhari Ida Parwati Ida Parwati Iman Rahayu Irvan Afriandi Irvan Afriandi Jeffery Malachi Candra Jose RL Batubara Juhariah Juhariah Julia Hartati Koesoemadinata, Raspati C. Korry Novitriani Krishna Yana Krisnian, Tharani Kumia Wahyudi Kusumawardhani, R.N. Yasmin Lelly Yuniarti Leonardus Wiydatmoko Loe, Luse marni banowati Menik Herdwiyanti Muhamad Zulchoir Rasyid Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Nanan Sekarwana Nanny Natalia Mulyani Soetedjo Nanny Natalia Mulyani Soetedjo Ni Sayu Dewi B. Nina Dwi Putri Nisa Fauziah Novi Jayanti Nuni Sulastri Nurizzatun Nafsi Nurul Hidayah Chairunnisa Nururrohmah, Siti Nury Fitria Dewi obin sarwita Panji Fortuna Hadisoemarto Parse, Rocci Jack Prayudi Santoso Putri Vidyaniati Ratna Yulistya Ningrum Reinout van Crevel Reinout van Crevel Ressa Novita Afandi Resvi Livia Rina Triasih Rocci Jack Parse Rovina Ruslami Rovina Ruslami, Rovina Santoso, Ida Parwati Sari Syahruni Setyaningrum, Sinta Siti Soidah Sofia Imaculata Stephen M. Graham, Stephen M. Sulaiman Ngongu Depamede Suparman Hardinsyah, Suparman Susan Margaret McAllister Susandi, Evan Susantina Prodjosoewojo Syndi Nurmawati Teow Sheng Hao Tharani Krisnian Tjahjani M. Sudiro Tony Sadjimin Toto Subroto Toto Subroto Toto Subroto Trinugroho Heri Fadjari, Trinugroho Heri Trisasi Lestari, Trisasi Tryawan, Ilham Ngongu Tya Listiaty Ulfah Dwi Febriani Umi Baroroh Uun Sumardi, Uun Willem J Gerver Wirawan, Chevie Woro Indri Padmosiwi Yana, Krishna Yani Triyani Yani Triyani Yanti Mulyana Yeva Rosana Yovita Hartantri Yulniar Tasli