Article Per Year (5 Year)
p-Index From 2021 - 2026
1.116
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Microbiology Indonesia Pharmaciana: Jurnal Kefarmasian Sari Pediatri Paediatrica Indonesiana Biota Jurnal Ilmu Kefarmasian Indonesia Pharmaceutical Sciences and Research (PSR) Makara Journal of Science
Amarila Malik
Faculty Of Pharmacy, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
Characterization of Trypsin-Like Protease of Lactobacillus plantarum FNCC 0270 Trismilah Margono; Wahono Sumaryono; Amarila Malik; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 21 No. 2 (2014): June 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1468.621 KB) | DOI: 10.4308/hjb.21.2.87

Abstract

Trypsin is an enzyme that has a unique mechanism of cutting peptide bonds specifically at the carboxyl side of lysine or arginine amino acids, with another amino acid. This study aims to analyze a trypsin-like protease (TLP) found in Lactobacillus plantarum FNCC 0270,  by performing partial proteomic tests, i.e. MALDI-TOF/TOF, and standard bioinformatics tools. SDS-PAGE analysis showed 4 protein bands. Two bands of the (P1 and P2) showed molecular weights equivalent to 47.35 and 38.42 kD, each generating 8 and 11 peptide fragments respectively. According to information in www.ncbi.nlm.nih.gov/genbank/structures, the structure  of serine protease HtrA (subs. plantarum L. plantarum ST–III) consists of three domains. Using Clone Manager® software by aligning two sequences we obtained eleven. The Lactobacillus produces of the trypsin-like serine protease has 40-90% similarity. Using the Clustal W2 software we passed the 11 sequences through multiple alignments, and found that the isolate L. plantarum is closely related to L. buchneri, L. brevis, and L. malefermentans on the phylogenetic tree. Alignment analysis results showed that all 8 peptide fragments of band 1 and 11 peptide fragments of band 2,  of the SDS-PAGE, were located in the active domain region of  the fourth trypsin-like serine protease producing Lactobacilli.
Sucrase Activity and Exopolysaccharide Partial Characterization From Three Weissella confusa Strains Amarila Malik; Sheilla Sheilla; Wangi Firdausi; Tri Handayani; Endang Saepudin
HAYATI Journal of Biosciences Vol. 22 No. 3 (2015): July 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.029 KB) | DOI: 10.4308/hjb.22.3.130

Abstract

Exopolysaccharides (EPSs) produced by lactic acid bacteria have been well known for their important economic value in food, pharmaceutical and health industries. Large extracellular enzyme sucrases are used by lactic acid bacteria to polymerize EPS, i.e. fructansucrase and glucansucrase. This study aimed to characterize sucrase activity of three Weissella confusa strains MBF8-1, MBF8-2 and MBFCNC-2(1), which were isolated previously from local beverages and their EPS products as well. All strains showed ability to form mucoid and slimy colonies by visual inspection on agar plate using raffinose as substrate suggesting that they possessed fructansucrase activity besides glucansucrase. Obtained EPS products were characterized by HPLC analysis after hydrolysis using 3% TCA at 100C for 1 hour, and by viscosity as well. All strains exhibited similar peak patterns, assuming that all of them possessed fructan EPS product. Supernatant and cell pellet were also analyzed by in situ activity assay performing periodic acid Schiff staining after polyacrylamide gel electrophoresis; only cell pellet showed sucrase activity. Viscosity observation showed that EPS products from all strains were able to increase the viscosity slightly.
Profiling Skin Microbiome in Healthy Young Adult Representing Javanese, Papuans, and Chinese Descent in Indonesia Stella Vania; Amarila Malik
HAYATI Journal of Biosciences Vol. 28 No. 4 (2021): October 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.4.249-261

Abstract

Skin serves as the first physical barrier and biological barrier by the colonization of commensal bacteria to prevent pathogen invasion. It was known that the disruption on normal commensal microbiota composition or dysbiosis causes skin diseases, while the skin microbiota diversity itself is influenced by several factors, one of them is ethnicity. This study shows the influence of ethnicity factor in Papuans, Javanese, and Chinese descent young adults living in Jakarta on skin microbiome profiles. The microbiota genomic DNA are extracted from the face skin samples and sequenced with Next Generation Sequencing method to be further analyzed. The result shows that individuals with the same ethnic background share similar skin microbiome characteristics. The greatest skin microbiome alpha diversity is shown by the Papuans and the Chinese descent the smallest. Ethnicity factor that shows statistically significant differences in interindividual dissimilarities are independent of other intriguing factors such as age, geographical location, etc. Therefore the ethnic origin of individuals especially from three ethnics above is a factor to be considered in skin microbiome research and the skin microbiota composition can be used for potential future applications.
In Silico and In Vitro Inhibitory Activity of Indonesian Herbal Compound Extracts against SARS-COV-2 Recombinant Papain-Like Protease Fatiningtyas, Fairuz Andini; Napitupulu, Riswanto; Malik, Amarila; Helianti, Is
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.356-366

Abstract

The SARS-CoV-2 papain-like protease (PLpro) is essential for viral replication and a promising target for drug discovery. This study explored the inhibitory potential of compounds from Indonesia herbals Butterfly pea flower (Clitoria ternatea L), Star fruit leaves (Averrhoa carambola L.), and Java plum leaves (Syzygium cumini (L.) Skeels) against PL pro through molecular docking and in vitro assays. The molecular docking method utilized the target protein PLpro (PDB ID: 7CMD), with the native ligand obtained from compounds identified in these plant extracts. The compounds were identified using the KNApSAcK database and analyzed for drug-likeness based on Lipinski's Rule of Five. The physicochemical characteristics affecting absorption, distribution, metabolism, excretion, and toxicity (ADMET) were determined using the pkCSM descriptor algorithm protocol. Validation was performed using the redocking method, achieving an RMSD score of 0.728 Å, which indicated validity (RMSD <2.0 Å). The results identified four ligands with the lowest binding affinities from these extracts: (-)-Epicatechin 3-O-gallate, folic acid, petunidin 3-glucoside, and ellagic acid, with binding scores of -8.6, -8.3, -7.1, and -7.1 kcal/mol, respectively. Prior to conducting the PLpro in vitro inhibition assay, a fluorescence-based inhibition assay was performed using Z-RLRGG-AMC as the substrate and GRL0617as the control inhibitor. All extracts were subjected to 70% ethanol maceration. The IC50 value of GRL0617 was 3.38 μM, while fluorescence tests showed that Java plum leaf extract exhibited the highest inhibition percentage at 66.10±3.22%. These findings indicate that all three plant extracts contain compounds capable of inhibiting PLpro activity.
Co-Authors 'Alawiyyah, Raden Zulfa Achmad, Fariz Ahmad Faried Ajitya Kurnia Hermawati Amin Soebandrio Anandayu Nurfachtiyani ANDI YEHUDA Andiani Wanda Putri Anis Karuniawati Arry Yanuar Asmarinah Asmarinah Astutiati Nurhasanah ASTUTIATI NURHASANAH Atiek Soemiati Aulia Rizki Donna M. Ariestanti Dumingan, Alvian Endang Saepudin Endang W. Bachtiar Fatiningtyas, Fairuz Andini HANIFAH RAHMI HANS-JOACHIM FREISLEBEN HARALD HUBER Hermawati, Ajitya Kurnia Hestiningtyas, Mahardhika Huda, Fathul IMAN SANTOSO IS HELIANTI Kambang Sariadji Kusmiati . Kusmiati Kusmiati Kusmiati Kusmiati Larashintya Rulita Mahardhika Hestiningtyas Maksum Radji Maksum Radji Mohamad Sadikin Mohamad Sadikin Murti Andriastuti, Murti Napitupulu, Riswanto Nilam Sartika Noverina, Rachmawati Nusaiba, Putri Oktaviyani, Devi Purwaningtyas, Yoggi Ramadhani Putri, Ratih Rinendya Radhian Amandito Radhian Amandito Ratih Rinendyaputri Retnaningsih, Agustina Rina Mutiara Rinawati Rohsiswatmo Rosari Saleh S.Pd. M Kes I Ketut Sudiana . SELA SEPTIMA MARIYA Septelia Inawati W SEPTELIA INAWATI WANANDI SERUNI K.U. FREISLEBEN Sheilla Sheilla Sri Wulandah Fitriani Stella Vania Sukma Nuswantara Sukma Nuswantara Sunarno Sunarno Sunarno Sunarno Susan Rahayu Syafrida Siregar Syafrida Siregar, Syafrida Tiodinar Theresia Tri Handayani Trismilah Margono Trismilah Trismilah Trismilah, Utama, Hieronimus Adiyoga Nareswara Wahono Sumaryono Wangi Firdausi ZESSINDA LUTHFA