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Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Abstract

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.
Mass Propagation of Antagonistic Bacteria Pseudomonas fluorescens as an Environmental-Friendly Biocontrol Agent Yosua Nathanael Itona Sagala; Ramadhani Mahendra Kusuma; Suharto
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3211

Abstract

Biological control is a long-term and environmentally friendly method of pest management. The use of biological control agents has several advantages. These advantages include limiting the growth and development of plant-disrupting organisms over a relatively long period. Furthermore, biological agents have advantages in maintaining the equilibrium of the ecosystem that is present in agricultural environments. Due to their ability to create antimicrobials and stimulate plant development, as well as the fact that they are plant growth-promoting rhizobacteria (PGPR) and can survive in adverse environmental circumstances, antagonistic bacteria are one of the biological or biocontrol agents for managing illness. Antagonizing microorganisms can boost plant resilience to disease attack without polluting the environment or leaving toxic residues in the food chain Bacteria with good antagonistic agent properties can prevent the growth of pathogens and stimulate plant resistance responses. In addition, antagonistic bacteria can simultaneously boost the growth response of plants (plant growth promoter). One of the antagonistic bacteria used as PPGF is Pseudomonas fluorescens. Pseudomonas fluorescens has been widely recognized as having the potential as a biological agent to inhibit several plant pathogens. P. fluorescensis a group of root bacteria that effectively suppresses various plant diseases, including damping off of seedlings, soft rot, bacterial wilt, and others on many plant varieties. An antibiotic substance produced by P. fluorescens. (2,4-diacetylphloroglucinol/ 2,4-DAPG) can increase soil resistance to pathogens.
Diversitas Arthropoda Herbivora pada Tumbuhan Liar dalam Lingkungan Budidaya Tanaman Tomat Pratama, Kevin Dimas; Windriyati, Wiwin; Kusuma, Ramadhani Mahendra
RADIKULA: Jurnal Ilmu Pertanian Vol 3 No 2 (2024): RADIKULA - Desember 2024
Publisher : Fakultas Sains dan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/radikula.v3i2.5574

Abstract

Keanekaragaman Arthropoda herbivora pada tumbuhan liar perlu dikaji untuk memahami dinamika keseimbangan ekosistem. Penelitian ini bertujuan untuk mengidentifikasi jenis Arthropoda herbivora serta menganalisis keanekaragaman, dominansi, kemerataan, dan kekayaan jenis yang ditemukan pada tumbuhan liar di sekitar budidaya tomat. Metodologi yang digunakan melibatkan pengambilan sampel Arthropoda herbivora dari tumbuhan liar menggunakan metode perangkap dan pengamatan langsung. Indeks keanekaragaman Shannon-Wiener (H'), indeks dominansi (C), indeks kemerataan jenis (E), dan indeks kekayaan jenis (Dmg) dihitung untuk menentukan tingkat keanekaragaman dan distribusi spesies. Hasil penelitian menunjukkan bahwa terdapat 33 genus Arthropoda herbivora dengan total 938 individu. Nilai indeks keanekaragaman Shannon-Wiener (H') adalah 2,79, menunjukkan kategori keanekaragaman sedang. Indeks dominansi (C) sebesar 0,09 mengindikasikan dominansi rendah, sedangkan indeks kemerataan jenis (E) sebesar 0,80 menunjukkan penyebaran spesies yang merata. Indeks kekayaan jenis (Dmg) sebesar 4,68 masuk dalam kategori sedang.
Population and Level of Noctuidae Pest Attack on Sweet Corn (Zea Mays Saccharata Sturt.) Agustin, Eva Tri; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15349

Abstract

Background: Sweet corn (Zea mays saccharata Sturt) is a food plant from the family grass, and cultivating sweet corn is more profitable because it has high economic value on the market. The growth phase of sweet corn plants often experiences a decrease in production yields, even crop failure due to pest attacks. This research aims to determine the pest population and the level of Noctudiae pest attacks that attack sweet corn plants. The study was conducted on moorland in Pilanggot Hamlet, Wonokromo Village, Tikung District, Lamongan Regency, East Java, from May to July 2023. This research used a selected random sampling survey method, namely observations on five plots, and each plot contained 48 observed plants. Observations began when the sweet corn plants were 14 HST and carried out once a week, 10 observations with 7-day intervals, namely in the vegetative phase (14, 21, 28, 35, 42) and generative phase (49, 56, 63, 70 and 77 HST). ). The research results show that the highest population of Noctuidae pests is the armyworm (Spodoptera frugiperda), with as many as 63 individuals with a relative abundance of 54%. The Noctuidae pests found were the armyworm (Spodoptera frugiperda), moth (Spodoptera litura), and cob borer (Helicoverpa armigera). The highest attack percentage is 25%, but it is still mild.
Morphological and molecular characterization of Stenocranus pacificus (Hemiptera: Delphacidae) on maize plants Fachmi, Anivea; Megasari, Dita; Kusuma, Ramadhani Mahendra
Jurnal Ilmiah Pertanian Vol. 21 No. 3 (2024): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v21i3.20215

Abstract

Maize (Zea mays L.) is one of the primary food commodities with a strategic role in improving Indonesia's economy. Additionally, maize contributes to food diversification and security. One of the main threats to maize production is pest attacks, including Stenocranus pacificus and Peregrinus maidis. This study aims to identify S. pacificus (Hemiptera: Delphacidae) found on maize crops in three sub-districts of Lamongan Regency, East Java, using both morphological and molecular approaches. Morphological identification revealed that the planthopper found is S. pacificus, characterized by an orange abdomen in male imagos and a white abdomen in female imagos. Molecular testing through polymerase chain reaction (PCR) using mitochondrial Cytochrome Oxidase I deoxyribonucleic acid (mtCOI DNA) showed that the S. pacificus from Lamongan is 99.53% similar to S. pacificus WRJJ_UNILA and WRJB_UNILA. These findings are expected to support more accurate, rapid, and effective pest control strategies.
PKM Peran Mahasiswa Kampus Mengajar 3 dalam Meningkatkan Literasi dan Numerasi Peserta Didik di Sekolah Dasar Fitriyani, Novi Nur; Kusuma, Ramadhani Mahendra; Supriadi, Yudi Nur; Kusuma, Jaka Wijaya; Hamidah, Hamidah
International Journal of Community Service Learning Vol. 6 No. 2 (2022): May 2022
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v6i2.51914

Abstract

Kampus Mengajar merupakan bagian dari program kebijakan Merdeka Belajar oleh Kementerian Pendidikan, Kebudayaan, Riset, dan Teknologi Republik Indonesia yang memberikan kesempatan kepada mahasiswa selama 1 (satu) semester untuk membantu guru dan kepala sekolah SD dan SMP dalam melaksanakan kegiatan pembelajaran yang terdampak Pandemi. Tujuan dari penelitian ini yakni untuk meningkatkan kemampuan literasi dan numerasi siswa Sekolah Dasar melalui pelaksanaan program kampus mengajar. Penelitian ini tergolong kedalam jenis penelitian pengabdian kepada masyarakat. Mitra yang terlibat dalam pengabdian ini yakni siswa sekolah dasar serta dewan guru. Pengabdian ini dilaksanakan dalam tiga tahap yakni persiapan dengan mengikuti pembekalan dan melakukan observasi sekolah penempatan; pelaksanaan program Kampus Mengajar; dan melakukan analisis hasil pelaksanaan program Kampus Mengajar. Pendekatan yang digunakan dalam penelitian ini yakni pendekatan kualitatif dengan metode deskriptif. Dalam teknik pengumpulan data, penulis menggunakan teknik dokumentasi dari setiap kegiatan yang dilaksanakan kemudian ditinjau dari beberapa daftar pustaka. Hasil pengabdian menunjukkan bahwa terdapat perubahan dan dampak yang baik bagi siswa, guru maupun sekolah seperti meningkatnya kualitas keterampilan membaca siswa, meningkatnya kualitas numerasi yang berkaitan dengan belajar perkalian, pembagian, pengurangan dan penjumlahan, mewadahi kegiatan bermain sambil belajar, memfasilitasi ruang baca, melakukan pendampingan belajar serta membantu adaptasi teknologi dan keadminitrasian. Masing-masing peserta didik terlihat antusias dan sungguh-sungguh dalam pelaksanaan kegiatan. Peserta didik juga terdorong untuk aktif dan terdorong dirinya untuk memacu semangat dan motivasi belajar.
Strategy to Increase Sales through Rebranding and Diversification of Ginger Syrup "Dwi Dewii" Safeyah, Muchlisiniyati; Mandasari, Virginia; Kusuma, Ramadhani Mahendra
Plakat : Jurnal Pelayanan Kepada Masyarakat Vol 6, No 2 (2024): Volume 6, Nomor 2 Desember Tahun 2024
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/plakat.v6i2.17054

Abstract

This article examines the strategy to increase the sales of "Dwi Dewii" ginger syrup in Kelurahan Bendo through rebranding and product diversification. Ginger syrup, known for its health benefits such as boosting immunity, faces challenges in marketing and sales, primarily due to conventional marketing strategies that only target adult consumers and are less appealing to younger audiences. This program aims to identify the gaps in current marketing strategies and develop new methods to enhance the product’s appeal and sales. The methodology used includes observation, interviews, and an analysis of existing packaging designs and marketing techniques. This community service project involved four lecturers and three students, collaborating with the primary partner, "Dwi Dewii" Ginger Syrup small-scale enterprise, and four other local small-scale enterprises producing traditional Blitar souvenirs in Kelurahan Bendo. The steps taken included redesigning the product packaging, providing training in digital marketing, and product diversification. Rebranding and diversification are expected to increase market appeal and broaden the consumer base, especially among younger demographics. The new packaging design aims to enhance the product's quality perception and attract a wider range of consumers. Product diversification is anticipated to improve customer satisfaction and boost sales volume. Furthermore, optimizing digital marketing through social media and e-commerce has proven effective in increasing visibility and consumer interaction, ultimately contributing to the economic growth of the partner small-scale enterprises.Artikel ini mengkaji strategi peningkatan penjualan produk sirup jahe "Dwi Dewii" di Kelurahan Bendo melalui pendekatan rebranding dan diversifikasi produk. Sirup jahe yang dikenal karena manfaat kesehatannya, seperti meningkatkan imunitas, menghadapi tantangan dalam hal pemasaran dan penjualan. Hal ini terutama disebabkan oleh strategi pemasaran konvensional yang hanya menjangkau kalangan usia dewasa dan kurang menarik bagi konsumen usia remaja. Program ini bertujuan untuk mengidentifikasi permasalahan dalam strategi pemasaran yang ada serta mengembangkan metode baru yang dapat meningkatkan daya tarik dan penjualan produk. Metode yang digunakan meliputi observasi, wawancara, dan analisis terhadap desain kemasan serta teknik pemasaran yang sedang diterapkan. Kegiatan pengabdian ini melibatkan empat dosen dan tiga mahasiswa, yang bekerja sama dengan mitra utama, yaitu UMKM Sirup Jahe Merah "Dwi Dewii", serta empat UMKM lainnya yang memproduksi oleh-oleh khas Kota Blitar di Kelurahan Bendo. Langkah-langkah yang dilakukan mencakup perancangan ulang kemasan produk, pelatihan pemasaran digital, dan diversifikasi produk. Rebranding dan diversifikasi diharapkan mampu meningkatkan daya tarik pasar serta memperluas segmentasi konsumen, termasuk kalangan remaja. Desain kemasan baru dirancang untuk memperbaiki citra kualitas produk dan menarik perhatian konsumen yang lebih luas. Diversifikasi produk diharapkan dapat meningkatkan kepuasan pelanggan dan volume penjualan. Selain itu, optimalisasi pemasaran digital melalui media sosial dan e-commerce dinilai efektif dalam meningkatkan visibilitas dan interaksi dengan konsumen, yang pada akhirnya berkontribusi pada pertumbuhan ekonomi mitra UMKM.
EFEKTIVITAS BERBAGAI KONSENTRASI BIOPESTISIDA FOBIO DALAM MENGHAMBAT PERKEMBANGAN PENYAKIT BLAS (Pyricularia oryzae) PADA TANAMAN PADI GOGO ORGANIK Deviyanti, Nur Qomariyah; Wiyatiningsih, Sri; Kusuma, Ramadhani Mahendra
Agrika Vol. 18 No. 2 (2024)
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v18i2.6199

Abstract

ABSTRAK Penyakit blas yang disebabkan oleh Pyricularia oryzae Cavara merupakan salah satu penyakit paling serius yang mempengaruhi produksi padi (Oryza sativa L.) di seluruh dunia. Terdapat strategi pengendalian dalam pertanian organik yang dapat dilakukan salah satunya yaitu penggunaan biopestisida yang dapat menekan perkembangan penyakit tanaman. Penggunaan biopestisida Fobio diharapkan mampu menghambat perkembangan P. oryzae penyebab blas padi karena mengandung berbagai mikroorganisme, baik yang sengaja diisolasi dari perakaran tanaman maupun yang terkandung didalam medium pembawanya. Tujuan penelitian untuk mengetahui efektivitas berbagai konsentrasi biopestisida Fobio dalam menghambat perkembangan patogen P. oryzae penyebab penyakit blas pada tanaman padi gogo organik. Penelitian menggunakan rancangan acak kelompok (RAK) dengan 4 perlakuan dan kontrol (0 ml/liter), FA (konsentrasi 5 ml/liter), FB (konsentrasi 7.5 ml/liter), dan FC (konsentrasi 10 ml/liter) dengan dosis masing-masing 8 liter per plot. Hasil penelitian menunjukkan bahwa perlakuan berbagai konsentrasi biopestisida Fobio mampu menghambat pertumbuhan jamur P. oryzae dengan persentase berbeda-beda dan perlakuan dengan konsentrasi 7.5 ml/liter (FB) merupakan perlakuan yang paling efektif menghambat serangan penyakit blas yang memiliki persentase intensitas keparahan penyakit terendah yaitu 55.4 %.  ABSTRACTBlast disease caused by Pyricularia oryzae Cavara is one of the most serious diseases affecting rice (Oryza sativa L.) production throughout the world. There are control strategies in organic farming that can be implemented, one of which is the use of biopesticides to suppress the development of plant diseases. The use of Fobio biopesticide is expected to inhibit the development of P. Oryzae, which causes rice blast, due to the various microorganisms it contains, both those deliberately isolated from plant roots and those contained in the carrier medium. The aim of the research is to determine the effectiveness of various concentrations of Fobio biopesticide in inhibiting the development of the pathogen P. oryzae that causes blast disease in organic upland rice plants. The study used a randomized block design (RBD) with 4 treatments and a control: control (0 ml/liter), FA (concentration 5 ml/liter), FB (concentration 7.5 ml/liter), and FC (concentration 10 ml/liter), each applied at a dose of -8 liters per plot. The research results show that treatment with various concentrations of Fobio biopesticide was able to inhibit the growth of P. oryzae fungi to varying degrees, with the concentration of 7.5 ml/liter (FB) being the most effective treatment, resulting in the lowest percentage of disease severity intensity at 55.4 %. 
Fostering Biodiversity: Unleashing the Potential of Refugia to Enhance Arthropod Diversity in Chili (Capsicum annuum L.) Farms Reksiana, Catur Putri Enggit; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12350

Abstract

Background: The utilization of Integrated Pest Management (IPM) offers a solution to chili cultivation pest challenges by implementing ecological engineering strategies, such as planting refugia. These refugia serve as SNAP (Shelter, Nectar, Alternative Food, and Pollen) sources for beneficial insects. This study aimed to assess the impact of refugia planting on arthropod-type diversity and explore the influence of abiotic factors on arthropod populations. Methods: Conducted from February to May 2023, the research utilized various traps (sweep net, yellow trap, pitfall trap, and light trap) and visual control for specimen collection. Results: The identification revealed the presence of three classes, ten orders, and 31 families. Arthropod populations in chilli fields with refugia totaled 867 individuals, whereas those without refugia amounted to 475. Conclusions: Planting refugia led to an elevated diversity index and evenness of arthropods, coupled with a lower dominance index compared to fields without refugia. Surprisingly, temperature and air humidity did not exhibit a significant impact on the arthropod population in chilli fields.
The Increase of Insect Pest Population in Paddy Field Managed with an Ecological Approach using Refugia Plants and Biopesticides Alprilia, Arieska Wahyu; Windiyanti, Wiwin; Kusuma, Ramadhani Mahendra
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 1 (2024): June 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.1.31-41.2024

Abstract

Insect pests can damage plants and act as vectors of diseases such as brown planthoppers which can transmit dwarf virus disease. The presence of insect pests is influenced by the type of variety, planting patterns, and excessive use of chemical pesticides so that insect pests increase and are difficult to control. This research wanted to provide insights into the effectiveness of combining insectary plants and biopesticides as ecological strategies to manage paddy (Oryza sativa Linnaeus) insect pests. The study was conducted in February-April 2024 in Sukodadi Village, Sukodadi District, Lamongan Regency. Method of study was conducted using a combination of direct observation, insect traps, and quantitative analysis to evaluate the impact of refugia plants and biopesticides on insect pest populations in paddy fields. The analysis includes the species diversity index (Shannon-Winner), species evenness index, species richness index (Margalef), dominance index (Simpson) and similarity index (Bray-Curtis). The study obtained 2,687 individual insect pests in paddy fields with refugia and fobio and 1,761 individuals in paddy fields without refugia and fobio. Insect pests were more abundant in paddy fields with refugia and fobio compared to fields without refugia and fobio. Refugia provides alternative habitats and additional resources for insect pests, and the use of biopesticides can create temporary ecosystem imbalances, and increase the population of insect pest.