Article Per Year (5 Year)
p-Index From 2020 - 2025
1.347
P-Index
This Author published in this journals
All Journal E-Jurnal Medika Udayana Medical Journal of Indonesia Majalah Kesehatan FKUB JKKI : Jurnal Kedokteran dan Kesehatan Indonesia eJournal Kedokteran Indonesia
I Gusti Kamasan Nyoman Arijana
Bagian Histologi Fakultas Kedokteran Universitas Udayana
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Medical Journal of Indonesia

 1 
NF-κB, neutrophil extracellular traps, and microglial in mice with Streptococcus suis serotype 2 meningitis infection Susilawathi, Ni Made; Adi Tarini, Ni Made; Arijana, I Gusti Kamasan Nyoman; Sriwidyani, Ni Putu; Pramitasuri, Tjokorda Istri; Sudewi, Anak Agung Raka; Mahardika, I Gusti Ngurah Kade
Medical Journal of Indonesia Vol. 33 No. 3 (2024): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247394

Abstract

BACKGROUND Streptococcus suis is the most frequent etiology of zoonotic bacterial meningitis, potentially initiating an outbreak. Acute bacterial meningitis caused by S. suis has various manifestations, often accompanied by sepsis with multiple organ involvement. This study aimed to evaluate the pattern of S. suis outgrowth in the brain, which is associated with nuclear factor-κappaB (NF-κB) activation, neutrophil extracellular traps (NETs) release (NETosis), and microglial activation as three crucial pathological mechanisms of bacterial meningitis. METHODS This study used 64 female BALB/c mice aged 6 weeks and weighed 18−20 g, grouped into infected and non-infected as the control group. Both groups were administered 1 ml of S. suis serotype 2 suspension (1 × 107 colony forming-unit/ml) and normal saline intraperitoneally. The bacterial colony count of S. suis was evaluated, along with NF-κB and NET levels in blood and brain, as well as meningeal inflammation and microglial activation in the brain at Days 1, 3, 5, and 7 post-infection. RESULTS The invasion of S. suis into the brain slightly induced NF-κB activation, leading to a burst of inflammatory responses, neutrophil infiltration with NET releases, and microglia activation that co-occurred, showing their peaks on Days 3 and 5 after onset. CONCLUSIONS The S. suis invasion into the mice’s brain increased NF-κB activation, NETosis, and microglia activation during S. suis meningitis infection.
Co-Authors Adnyana, I Made Diatmika Agus Eka Darwinata Dewi Wijayanti, Ni Putu Ayu I Gde Haryo Ganesha I Gusti Ayu Dewi Ratnayanti I Gusti Ayu Dewi Ratnayanti I Gusti Ngurah Kade Mahardika I Gusti Nyoman Sri Wiryawan I Gusti Nyoman Sri Wiryawan I Nyoman Wande I Wayan Juli Sumadi I Wayan Rai Widarta I Wayan Sugiritama Ida Ayu Ika Wahyuniari Kenjiro, Eduardo Luh Putu Ratna Sundari Luvianto, Juven Ni Luh Gede Yoni Komalasari, Ni Luh Gede Yoni Ni Made Adi Tarini Ni Made Linawati Ni Putu Sriwidyani Pramitasuri, Tjokorda Istri Raka-Sudewi A. A. Ratnayanti, Gusti Ayu Dewi Sidauruk, Dona Uli Susilawathi, Ni Made