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All Journal Media Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences

I Gusti Agung Arta Putra

Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 233946

Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.

Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang I Gusti Agung Arta Putra; Dondin Sajuthi; Dedy Duryadi Solihin; Raden Roro Dyah Perwitasari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .

Ekstrak Kulit Buah Manggis (Garcinia mangostana Linn) Mampu Meminimalkan Efek Imunosupresif Monosodium Glutamate (EFFECTIVENESS OF MANGOSTEEN PEEL EXTRACT (GARCINIA MANGOSTANA LINN) IN MINIMIZING THE IMMUNOSUPPRESSIVE EFFECT OF MONOSODIUM GLUTAMATE) Anak Agung Bagus Bramardipa; Anak Agung Ayu Mirah Adi; I Gusti Agung Arta Putra
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Monosodium glutamate (MSG) is commonly used as a food additive or flavoring worldwide, unfortunately MSG is reported to have a toxic effect to the immune system, that can lead to an immunosuppressive conditions. One of immunosuppressive signs in chickens is atrophy of bursa Fabricius (BF). Mangosteen peel extract contains active substances and other components that can act as an immunomodulator. The aim of this study was to evaluate the immunosuppressive effects of MSG and the effectiveness of mangosteen peel extract in minimizing the immunosuppressive effects of the MSG. This study used a completely randomized design with four treatments namely A, B, C and K consisted four chickens each. The chickens in treatment A, B, C and K were given feed containing MSG 20% W/W and mineral water for drinking, commercial feed and mineral water containing 2% W/V mangosteen peel extract, MSG 20% W/W and mineral water containing mangosteen peel extract 2% W/V and commercial feed and drinking water without mixture. (control), respectively. Based on the results of this study, it was found that the administration of feed containing 20% MSG could result in bF atrophy with a very low bF index (p <0.01) if compared with B, C, and K-treatment. Prominet histopathological (HP) features of bF of chickens under treatment A are follicular atrophy and interfollicular edema. Treatment with feed containing 20% MSG along with the administration of drinking water containing 2% mangosteen peel extract keeps on maintaining the bF under normal condition even microscopically was found macrophages proliferation inside the lymphoid follicle. Based on clinical symptoms, index of bF and histopathological features, it can be concluded that MSG has immunosuppressive effects on chicks and mangosteen peel extract in drinking water can minimize this effect.

Pembiusan Monyet Ekor Panjang (Macaca fascicularis) dengan Campuran Ketamine dan Xylazine (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY INJECTING THE COMBINATION OF KETAMINE AND XYLAZINE) I Nyoman Suartha; I Nengah Wandia; I Gusti Agung Arta Putra; I Gede Soma; I Gusti Ngurah Sudisma
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Infeksi Avian Paramyxovirus Tipe-1 pada Babi di Bali (AVIAN PARAMYXOVIRUS TYPE-1 INFECTION IN PIG IN BALI) Anak Agung Ayu Mirah Adi; I Gusti Agung Arta Putra; Bayu Setiabudi; I Made Kardena; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Avian Paramyxovirus type 1 (APMV-1) is a virus infecting birds with mortality level varies accordingto its pathotype. Although the virus is normally originated from birds, the recent report showed that it canbe isolated from pig. In order to find out APMV-1 infections on pig in Bali, a serologic study was conductedto detect the antibody against APMV-1 among pigs in Bali. Indirect enzyme-linked immunosorbentassay (ELISA) was used to detect the antibody against APMV-1 in pig sera. The serum samples withoptical density value over the cut off value (COV) was considered as those containing Ab against APMV-1. Of 215 samples collected from traditional farms of eight regency and city in Bali namely Negara,Tabanan, Badung, Denpasar City, Gianyar, Bangli, Karangasem and Buleleng Regency, 174 samples(80,93%) were positive antibody against APMV-1. On the other hand, there were 125 (98,42%) positivesample among 127 sample collected from Pesanggaran abattoir. Furthermore, the 8-9 month age pigsshowed that their antibody against APMV-1 were higher (p<0.01) than those of 3-6 months age. TheELISA positive samples were further confirmed using Western immunoblotting assay. The anti-APMV-1antibody positive sera reacted with the virus specific protein band of 258 kDa and the negative sera did notreact with the viral protein. As pig has never been vaccinated with APMV-1 vaccine, the presence of Abagainst APMV-1 showed that the pigs were naturally infected with APMV-1.

Variasi Genetik Gen Penyandi Protein Fusi dari Avian Paramyxovirus Tipe I di Bali (GENETIC VARIATION OF GENE ENCODING FUSION PROTEIN OF AVIAN PARAMYXOVIRUS TYPE-I IN BALI I Gusti Agung Arta Putra; Anak Agung Ayu Mirah Adi; Nyoman Mantik Astawa
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study aims to determine the genetic variation of gene encoding fusion (F)-protein of avianparamyxovirus (APMV) type-1 isolated from chickens suffering of Newcastle disease (ND) found in achicken farm in the province of Bali throughout the year 2014. There are five isolates got from sick chickenscases/death suspected of being infected by APMV -1, but negative for avian influenza virus (AIV) infectioni.e: D5/AK/2014, B1/AK/2014, T1/ARP/2014, G1/AK/2014, and K1/ARP/2014. Sequence analysis of thefive isolates showed that three isolates of D5, B1 and G1 have a genetic distance of 11,1%, 10,2% and 8,2%when compared to the nucleotide sequence of Bali-1/07 isolated previously from Karangasem regency in2007. Meanwhile, the genetic distance of T1 and K1 isolates are 20,1% and 18% respectively as compareto the sequence of the Bali-1/07 isolate. The composition of amino acids at the cleavage sites enzyme of Fproteinfor 3 isolates of D5, B1 and G1 are R-R-Q- K-R-F, which is the typical characteristic of virulentvirus strain. Meanwhile, two isolates i.e T1 and K1 got from layer chicken had the amino acid sequence ofG- R-Q-G -R-L, which is group into avirulent virus strains. It was concluded that APMV-1 isolates foundin ND cases in the year of 2014 were caused by virulent isolate and were grouped into VII-genotype.Furthermore, It was found that there were two avirulent isolates

Fraglilitas Eritrosit Anjing Lokal Bali (THE FRAGILITY OF ERYTHROCYTE ON LOCAL DOGS IN BALI) Siswanto Siswanto; I Gusti Agung Arta Putra; Ida Ayu Putri Utami; I Gede Soma
Jurnal Veteriner Vol 2 No 3 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Pembiusan Monyet Ekor Panjang (Macaca fascicularis) Jantan dengan Campuran Ketamin dan Xilazin pada Topografi Daerah Berbeda (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY KETAMINE-XYLAZINE ON DIFFERENT TOPOGRAPHIC AREA) I Nyoman Suartha; I Gusti Agung Arta Putra; I Nengah Wandia
Jurnal Veteriner Vol 4 No 1 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Establishment and Characterization of Benzo(a)pyrene-Induced Skin Tumor in Rats Palagan Senopati Sewoyo; Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Marissa Divia Dayanti; Anak Agung Bagus Bramardipa; I Ketut Eli Supartika
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Quite a number of research on cancer therapy strongly require an animal model of cancer. One of the chemicals commonly used to induce cancer in animal models is benzo(a)pyrene due to its carcinogenic effects. This study aims were to describe the gross pathology of the tumor-induced by benzo(a)pyrene in an olive oil solution (w/v), identify the type of tumor histopathologically, and finally, determine the correlation between the duration of the rats experiencing tumor and it’s grade score. Tumor grade score is important to assess in order to determine tumor malignancy. This study consisted of 10 white rats (Rattus norvegicus) were given two treatments: a negative control treatment (K-) was injected with 0.1 mL of olive oil and a positive control treatment (K+) was injected with 0.1 mL of 0.3 % (w/v) benzo(a)pyrene in olive oil solution. Each treatment rats was kept in a cage and monitored regularly. When the tumors macroscopically appeared in the interscapular area and were observed until reached 4 cm in size, the rats were then sacrificed and necropsied. Tumors were observed for the gross pathology to examine the shape and color of them, then routinely processed for histopathological evaluation. The results showed that the tumors’ cells appeared to be round (1/5), irregular (2/5), and multilobular (2/5). Based on histopathological observation, the types of tumors observed were classical fibrosarcoma (2/5) and pleomorphic fibrosarcoma (3/5). There is a significant association between the duration of the rats experiencing tumors and the tumor grade. The longer the rats have tumors, the tumors tend to be more aggressive.

Co-Authors Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Bagus Bramardipa Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Bayu Setiabudi Brotcorne, Fany Dewi Ayu Warmadewi Dondin Sajuthi DWI SURYANTO DYAH PERWITASARI -FARAJALLAH I Dewa Ketut Harya Putra I G. Lanang Oka Cakra I Gede Soma I Gusti Ngurah Sudisma I Ketut Eli Supartika I Ketut Puja I Ketut Suatha I Made Kardena i Nengah Wandia I Nyoman Ardika I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Wayan Bebas I Wayan Gorda I WAYAN SUBERATA I Wayan Wirata Ida Ayu Putri Utami Ida Bagus Oka Winaya Jefri Ndawa Rumba Marissa Divia Dayanti Muh. Sofyan Muna Yuniarti Ni Luh Gde Sumardani Nyoman Sadra Dharmawan Palagan Senopati Sewoyo Sewoyo, Palagan Senopati Siswanto - Siswanto Sofyan, Muh. Sri Anggreni Lindawati Sri Kayati Widyastuti Suranjaya I .Gd TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Wayan Sayang Yupardhi

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