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Journal : Journal of Science and Applicative Technology

Penambatan molekul senyawa turunan orizanol terhadap enzim 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reduktase Syaikhul Aziz; Nur Adliani; Sukrasno Sukrasno
Journal of Science and Applicative Technology Vol 4 No 1 (2020): Journal of Science and Applicative Technology June Chapter
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.567 KB) | DOI: 10.35472/jsat.v4i1.191

Abstract

Oryzanol has been reported to reduce serum total cholesterol (hypolipidemic agent) by inhibiting HMG-CoA reductase, an enzyme responsible for the metabolic pathway that produces cholesterol and isoprenoid. The purpose of this experiment is to determine the inhibition activity of oryzanol derivatives on HMG-CoA reductase by molecular docking. Four structure of oryzanol derivatives, Lanosteryl-ferulate, Brassicasteryl-ferulate, Lupeol-ferulate, and Cholesteryl-ferulate were used as ligands for molecular docking. The HMG-CoA reductase structure was obtained from protein data bank and the study was performed using AutoDock Tools as a molecular docking software. All oryzanol derivatives show binding affinity against HMG-CoA reductase. Lupeol-ferulate was predicted to be the best inhibitory activity against HMG-CoA reductase because of molecular docking.
Quantitative Profiling of Total Flavonoid Content in Cassava Leaf (Manihot esculenta Crantz) Varieties and Isolation of Flavonoid Compounds from a High-Flavonoid Variety Aziz, Syaikhul; Siringoringo, Vinta Marito; Mulyani, Isna; Ngatinem
Journal of Science and Applicative Technology Vol. 9 No. 1 (2025): Journal of Science and Applicative Technology June Chapter
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/jsat.v9i1.2190

Abstract

Cassava (Manihot esculenta Crantz) is widely utilized across Indonesia as both a staple food and a traditional medicinal resource, and its leaves are known to contain bioactive secondary metabolites such as flavonoids. This study aimed to quantify the total flavonoid content in several cassava leaf varieties and to isolate a flavonoid compound from the variety with the highest flavonoid content. Total flavonoid content was determined using UV-Visible spectrophotometry. The isolation of flavonoid compounds was carried out using vacuum liquid chromatography (VLC) and column chromatography (CC), with thin-layer chromatography (TLC) employed to monitor the separation results. The total flavonoid content of the Gajah, UJ-3 (Thailand), UJ-5 (Katsesart), Cimanggu dan Kaspro varieties were found to be 1.029 ± 0.003 mgQE/g, 0.746 ± 0.006 mgQE/g, 0.970 ± 0.009 mgQE/g, 0.936 ± 0.004 mgQE/g and 1.220 ± 0.003 mgQE/g, respectively. The Kaspro variety exhibited the highest flavonoid content. Isolation and characterization of the flavonoid from Kaspro leaves yielded a yellowish powder with absorption maxima at 269 nm (Band II) and 334 nm (Band I). Further analysis using shift reagents identified the isolated compound as apigenin, a member of the flavone group.