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Journal : Buletin Peternakan

Optimalisasi Pengikatan Tanin Daun Nangka dengan Protein Bovine Serum Albumin (Optimalisation Binding of Jackfruit Leaves Tannin with Bovine Serum Albumin Protein) Wahidin Teguh Sasongko; Lies Mira Yusiati; Zaenal Bachruddin; Mugiono (Mugiono)
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i3.84

Abstract

Tannins are high molecular weight polyphenol compounds with ability to bind proteins. Based on the structure,albumin are simple globular molecule protein. Optimalisation binding of jackfruit leave tannins to bovine serum (BSA)albumin was done in two stages. The first stage was to determine levels of tannins and condensed tannins in jackfruit leaves grown in mediterranean soil types. Second research was optimalisation binding of jackfruit leaf tannins withbovine serum albumin. In the second stage there was the determination of protein-percipitable phenolics compound and protein content using Lowry method. The data showed total phenol content was 10.63%, total tannin was 7.08%, andcondensed tannins 5.57%. The optimum capacity phenolic compound to bind protein of bovine serum albumin was 5.71+0.18 mg BSA/100 mg dry matter of jackfruit leaf, while 1 g tannin could bind 23.149 g BSA or 1 g condensedtannin could bind 28.885 g BSA.(Key words: Tannin, Total phenol, Total tannin, Condensed tannin, Bovine serum albumin)
Karakteristik Bakteri Asam Laktat NWD015 Hasil Isolasi dari Feses Pedet dan Pengaruh Bakteriosin terhadap Bakteri Patogen Nafiatul Umami; Zaenal Bachruddin; Lies Mira Yusiati
Buletin Peternakan Vol 30, No 1 (2006): Buletin Peternakan Vol. 30 (1) Februari 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i1.1190

Abstract

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Aktivitas Enzim Carboxy Methil Cellulase dan Produksi Volatile Fatty Acid pada Fermentasi Selulosa oleh Mikrobia Rumen Secara In Vitro Nafiatul Umami; Zaenal Bachruddin; Hari Hartadi
Buletin Peternakan Vol 30, No 2 (2006): Buletin Peternakan Vol. 30 (2) Mei 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i2.1196

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Pemanfaatan Feses Kambing sebagai Sumber Mikrobia dan Enzim Mikrobia Pengganti Cairan Rumen Ristanto Utomo; Zaenal Bachruddin; Budi Prasetyo Widyobroto; Mohamad Soejono; Risa Antari
Buletin Peternakan Vol 30, No 3 (2006): Buletin Peternakan Vol. 30 (3) Agustus 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i3.1201

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Pengaruh Inokulasi Lactobacillus plantarum dan Saccharomyces cerevisiae terhadap Fermentasi dan Kecernaan In Vitro Silase Kulit Buah Kakao Muhammad Askari Zakariah; Ristianto Utomo; Zaenal Bachruddin
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9294

Abstract

The objective of this study was to identify the effect of L. plantarum and S. cerevisiae mixed culture inoculation into cocoa pods silage on chemical composition and in vitro digestibility. The four treatments were: 1 kg freshly harvested cocoa pods without inoculants as control (K); K + L. plantarum (KLp); K + S. cerevisiae (KSc); and K + L. plantarum and S. cerevisiae mixture (KLp+Sc) 0.1% dry matter, Cassava meal were added in all treatments. Each treatment was replicated 3 times, and then fermented for 21 days. Parameters observed in current study were gas test production, ruminal fermentation parameter, and in vitro digestibility. The collected data were analyzed by one-way analysis of variance and followed by Duncan’s new Multiple Range Test for data with significant differences. Result showed that the mixed culture Lp+Sc inoculation increased (P<0.05) chemical quality of cocoa pods by reducing fibre fraction and increase NFE contents, increased degradation rate, degradation theory, reduced rumen pH, and propionate acid production, without affecting acetate to propionate ratio, microbial protein synthesis, and digestibility of cocoa pod silage.
THE EFFECT OF MOLASSES, Lactobacillus plantarum, Trichoderma viride, AND ITS MIXTURES ADDITION ON THE QUALITY OF TOTAL MIXED FORAGE SILAGE Vian Dwi Chalisty; Ristianto Utomo; Zaenal Bachruddin
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.17337

Abstract

The objective of this research was to determine the effect of molasses, Lactobacillus plantarum, Trichoderma viride, and its mixtures addition on the quality of total mixed forage silage. Molasses was added 4% (w/w), L. plantarum 0,1% (v/w), and T. viride 0,1% (v/w). Each treatment was made 3 replication and then fermented for 21 days anaerobically. Variables measured were physical, chemical, and biological quality includes color, odor, texture, fungi, pH, lactic acid content, and ammonia. Data were analyzed using the analyses of variance Completely Randomized Designs. If there was significant among the treatment, it followed by a test of the average between two treatments with Duncan’s New Multiple Range Test. Test the quality of physical showed a yellowish green color, smell sour, dense texture, and the presence of the fungus little / no fungi. The addition of molasses alone or a mixture of molasses and L. plantarum/T. viride lowered pH and ammonia (P <0.05), while the lactic acid content increased (P <0.05). The addition of molasses or water-soluble carbohydrate is a must to produce good quality total mixed forage silage that shown with pH 3.60, lactic acid content 4.28% DM, ammonia content 0.43% DM.
EFFECT OF LIGNOSELULOLITIC FUNGUS TO ENZIMATIC ACTIVITY, FIBER FRCTION, AND DIGESTIBILITY ON FERMENTATION PROCESS OF COCOA POD Engkus Ainul Yakin; Zaenal Bachruddin; Ristianto Utomo; Ria Millati
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i3.22657

Abstract

The study was conducted to determine the enzyme activity, fiber fraction and digestibility in the fermentation process of cocoa pod. The substrate was used the cocoa pod while the fungi used Phanerochaete chrysosporium, Pleurotus ostreatus and Schizophyllum commune. Preparation of cocoa pod was chopped, finely ground and then dried. Preparation of fungi by growing fungi in liquid medium. Research methodology was the fermentation conducted with different fungi used four treatments and five replications. T1 = fermentation of cocoa pod without fungi addition, T2 = fermentation of cocoa pod with P. chrysosporium addition, T3 = fermentation of cocoa pod with  P. ostreatus addition, and T4 = fermentation of cocoa pod with S. commune. Fermentation used Erlenmeyer 250 ml and weight of cocoa pod was 100 gram. Fungi added on 5% from substrat weight bassis dry matter. Variables observed was enzyme activity, fiber fraction and digestibility . This study was designed using research design completely randomized design with a unidirectional pattern analysis of variance. Significant variables followed Duncan’s multiple range test. The results showed fermentation used P. chrysosporium has highest lignin peroxidase enzyme activity of 0.52±0.04 U/mL and mangan peroxidase 0.06±0.00 U/mL, neutral detergent fiber 75,54±0,41%, acid detergent fiber 68,10±0,30%, lignin 26,86±0,19%, cellulose 27,17±0,25%, hemicellulose 6,77±0,52%, dry matter digestibility 69,70±0,43% and organic matter digestibility 69,59±1,03%. The conclusion from this research that the fermentation by using fungi P. chrysosporium addition has the best result to degradated lignin. 
THE EFFECT OF LACTIC ACID BACTERIA AND DIFFERENT LEVEL OF CARBOHYDRATE SOURCES ADDITION ON TOFU WASTE INDUSTRY FERMENTATION Zaenal Bachruddin
Buletin Peternakan Vol 41, No 3 (2017): BULETIN PETERNAKAN VOL. 41 (3) AGUSTUS 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i3.23677

Abstract

A The purpose of this research was to figure out the affinity value of soluble carbohydrates from pollard with lactic acid bacteria (LAB) and its effect on tofu waste silage. Lactic acid bacteria (LAB) isolates used as inoculum were commerce LAB isolates (B13-1) and yolk LAB isolates (K6-3). The selected of LAB isolates used in the tofu waste silage were tofu waste (TW) and pollard (P) with different proportion (70:30), (60:40), and (55:45). The result showed that the addition of soluble carbohydrates sources in the tofu waste silage did not affect lactic acid and pH value. However LAB addition significantly increased lactic acid (P<0.05). The TW:P proportion (55:45) produced the highest lactic acid concentration with the value of 3.54%DM with pH value 3.90. Proportion of TW:P gave significantly different effect on dry matter (DM) and organic matter (OM) (P<0.01). Meanwhile, LAB addition presented significant difference in declining of dry matter percentage (P<0.01) but it showed non-significant effect on organic matter percentage from the tofu curd silage. It can be concluded that pollard addition with the value of 45% shows that tofu waste silage has the highest lactic acid concentration and ideal pH value.
The Potential of Hydrolysate from Rabbit Meat Protein as an Angiotensin Converting Enzyme Inhibitor Edy Permadi; Jamhari Jamhari; Edi Suryanto; Zaenal Bachruddin; Yuny Erwanto
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.31495

Abstract

This research aimed to investigate the rabbit meat hydrolysate potential as an angiotensin-converting enzyme (ACE) inhibitor. Indonesian local rabbit meats were used in this study. The research was conducted in Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, from August 2016 to February 2017. The local rabbit meats were hydrolyzed by pepsin, trypsin, and pancreatic. The obtained hydrolysates were then analyzed to identify the water-soluble protein content. The molecular weight of the hydrolysates were also confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The ACE inhibitory properties of the hydrolysates were analyzed in vitro. The results showed that pepsin, trypsin, and pancreatic hydrolysis showed a significant effect on the water-soluble protein content of rabbit meat (p<0.05). The water-soluble protein of rabbit meat hydrolysed by pepsin, trypsin, and pancreatic were 9.41, 7.66, and 9.75 mg/mL respectively. The molecular weight of the rabbit meat hydrolysate were increased from 10 to 43 kDa; 17 to 43 kDa; and 10 to 43 kDa, after hydrolysed by by pepsin, trypsin, and pancreatic respectively. Furthermore, the ACE inhibitory properties ) of the hydrolysed rabbit meat by pepsin, trypsin, and pancreatic were 439, 170, and 380 μg/mL, respectively. The rabbit meat hydrolysate showed a potential to be ACE inhibitor after hydrolyzed with pepsin, trypsin and pancreatic. Moreover, it also showed a promising potential to be used as bioactive components in different pharmaceutical applications. The highest ACE inhibitory capability was showed on trypsin hydrolysis with the total of 65.45% and 170 μg/mL ACE inhibition
Effect of Cashew Nutshell Oil Supplementation as Phenol Source for Protein Protection on In Vitro Nutrient Digestibility Rahma Fitriastuti; Lies Mira Yusiati; Budi Prasetyo Widyobroto; Zaenal Bachruddin; Chusnul Hanim
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.35591

Abstract

The current study was aimed to evaluate the ability of phenol content in cashew nutshell liquid (CNSL) in binding protein and its effect on in vitro nutrient digestibility. This research divided into three steps, there were determination of total phenol and tannins content in CNSL, optimalization binding of CNSL tannin to bovine serum albumin with the determination of protein-percipitable phenolics compound and protein content using Lowry method, after that amount of optimalization supplementation of CNSL was used for measuring on in vitro nutrient digestibility by Tilley and Terry method. The results showed that phenol and tannin content of CNSL were 148.69 g/100 ml and 28.3 g/100 ml. One gram tannin in CNSL could be bound by 65.83 g protein. Optimum tannin were used in this study was 5.85 g tannin or equivalent to 163.91 mg of CNSL. Amount of CNSL were used in various levels, there are 0, 50, 100, 150 dan 200 mg. CNSL supplementation reduced dry matter, organic matter crude protein digestibility in rumen, and didn’t reduce dry matter dan crude protein in vitro post rumen digestibility. Supplementation of CNSL increased feed efficiency on in vitro.