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All Journal Dentika Dental Journal Pro-Life Jurnal Pengabdian UntukMu NegeRI Eksakta : Berkala Ilmiah Bidang MIPA JURNAL FARMASIMED (JFM) Viva Medika: Jurnal Kesehatan, Kebidanan dan Keperawatan Best Journal (Biology Education, Sains and Technology) Journal of Functional Food and Nutraceutical Lumbung Farmasi : Jurnal Ilmu Kefarmasian Journal of Pharmaceutical and Health Research Jurnal Sains dan Teknologi Proceeding ISETH (International Summit on Science, Technology, and Humanity) Herbal Medicine Journal
Denny Satria
Department Of Pharmaceutical Biology, Faculty Of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
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Anticancer Potential of Methanol Extract and Fractions of African Leaf (Vernonia amygdalina Del.) Against HeLa Cervical Cancer Cells Suryati Cintia Gultom; Poppy Anjelisa Zaitun Hasibuan; Denny Satria
Jurnal FARMASIMED (JFM) Vol 8 No 2 (2026): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/yd7znx87

Abstract

Globally, cervical cancer is one of the leading causes of cancer-related mortality among women. African leaves (Vernonia amygdalina Del.) are known to contain active terpenoid compounds, such as sesquiterpene lactones, which can be utilized for cancer therapy. The aim of this study is to evaluate the cytotoxic potential of methanol extracts and leaf fractions from the African plant as anticancer agents against the HeLa cervical cancer cell line in vitro. The crude African leaf extract was prepared using a reflux method with methanol, followed by liquid-liquid fractionation to obtain n-hexane, chloroform, ethyl acetate, and residual fractions. Cytotoxicity testing was performed using the MTT Assay on HeLa cervical cancer cell lines; samples exhibiting the strongest cytotoxic activity underwent further testing, including cell cycle analysis and apoptosis assays using flow cytometry. The cytotoxicity test results showed that the methanol extract and fractions from African leaves exhibited cytotoxic effects with IC50 values ranging from 25.45 to 4163.63 μg/mL, with the chloroform fraction (IC50 = 25.45 ± 0.97 μg/mL) demonstrating the strongest cytotoxic activity. Cell cycle assay results showed that the chloroform fraction could inhibit HeLa cell proliferation in the G0-G1 phase. Cell apoptosis results indicated that the chloroform fraction could increase total apoptosis by 3.4%. Therefore, African leaves have potential as anticancer candidates, with anticancer active compounds tending to be concentrated in the chloroform fraction. Therefore, African leaves show potential as anticancer agents, with the active anticancer compounds tending to be concentrated in the chloroform fraction. Further studies are urgently needed to isolate and identify the pure compounds from the chloroform fraction of African leaves.
Potensi Seskuiterpen Lakton dari Vernonia amygdalina sebagai Inhibitor JAK1 pada Kanker Payudara Overekspresi HER-2 Hanifah Sabrina; Denny Satria
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 7, No 2 (2026): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v7i2.39217

Abstract

Jalur pensinyalan JAK/STAT memiliki peran penting dalam perkembangan kanker payudara yang menunjukkan overekspresi HER-2, yaitu tipe yang lebih agresif dan berkaitan dengan prognosis yang buruk serta ketahanan terhadap terapi. Penelitian ini menerapkan metode in silico untuk menyelidiki kapasitas senyawa seskuiterpen lakton dari Vernonia amygdalina sebagai penghambat JAK1. Sebanyak lima belas senyawa dianalisis dengan menggunakan Molecular docking dan simulasi Molecular Dynamics (MD) selama 100 ns. Validasi dari proses docking menunjukkan hasil yang memuaskan dengan nilai RMSD sebesar 0,616 Å. Vernolepin ditentukan sebagai senyawa utama karena memiliki afinitas pengikatan yang lebih baik dibandingkan dengan ruxolitinib (-8,2 kcal/mol) dan berinteraksi dengan residu-residu penting seperti Val889 dan Phe958. Hasil dari simulasi MD mengindikasikan bahwa kompleks JAK1-Vernolepin lebih stabil jika dibandingkan dengan ruxolitinib, terlihat dari nilai RMSD yang rendah (0,2-0,4 Å), RMSF yang konsisten, struktur yang kompak (Rg), nilai SASA yang stabil, serta rata-rata sekitar lima ikatan hidrogen. Analisis MM-PBSA juga menunjukkan bahwa energi pengikatan yang diperoleh lebih tinggi (105,82 kJ/mol). Temuan ini menegaskan bahwa vernolepin berpotensi menjadi kandidat sebagai inhibitor JAK1 untuk mengatasi ketahanan dalam kasus kanker payudara overekspresi HER-2.
Hepatoprotective Activity of Ethanol Extract of Mobe Leaves (Artocarpus lacucha Buch-Ham.) on Total Bilirubin, ALT, and AST Levels, and Macropathology of Liver in Rats Induced by Carbon Tetrachloride Meiva Amelia Lubis; Aminah Dalimunthe; Panal Sitorus; Denny Satria; Arga Lubis
Viva Medika Vol 17 No 3 (2024)
Publisher : LPPM Universitas Harapan Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35960/vm.v17i3.1490

Abstract

This study evaluates the hepatoprotective effects of ethanol extract of mobe leaves (Artocarpus lacucha Buch-Ham.) in rats induced with carbon tetrachloride (CCl4). The objective was to determine the extract's efficacy in reducing liver damage markers, specifically total bilirubin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels, and to assess liver macropathology. The ethanol extract was prepared by maceration using 96% ethanol. Results showed that administering 400 mg/kg body weight (BW) of the extract significantly reduced total bilirubin, ALT, and AST levels in CCl4-induced rats. Additionally, liver macropathology observations indicated improved liver conditions in the treatment group. Phytochemical screening revealed bioactive compounds like flavonoids, alkaloids, tannins, saponins, triterpenoids, and glycosides, which are potential antioxidants and hepatoprotective agents. The significant contribution of this research is providing a scientific basis for using mobe leaf extract as a natural hepatoprotective agent, highlighting its potential for safe and effective herbal medicine. Further research and clinical trials are recommended to validate these findings and explore the extract's therapeutic applications.
In Silico Evaluation of Cardiac Glycosides from Vernonia amygdalina as EGFR Inhibitors in Triple Negative Breast Cancer Gisela Agustin Habayahan; Denny Satria
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 02 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss02/676

Abstract

Triple-negative breast cancer (TNBC) is an aggressive subtype with limited targeted therapies, emphasizing the urgent need for novel molecular inhibitors. Epidermal growth factor receptor (EGFR) is overexpressed in TNBC, but current inhibitors show limited clinical efficacy, indicating a significant therapeutic gap. This study aims to evaluate cardiac glycosides from Vernonia amygdalina as EGFR inhibitors using an integrated in silico approach. Docking results demonstrated that vernoniosides A3 exhibited the lowest binding energy of -12.2 kcal/mol, outperforming the native ligand (-9.0 kcal/mol) and gefitinib (-8.5 kcal/mol). Molecular dynamics (MD) simulations revealed that EGFR-vernoniosides A3 complex remained stable, with RMSD values within stable range (0.7-3.8 Å), RMSF values (0.4-8.3 Å), radius of gyration (Rg) (6.0-7.0 Å), solvent-accessible surface area (SASA) (830-871 Ų), while forming up to 15 hydrogen bonds. However, MM-PBSA analysis showed that binding free energy of vernoniosides A3 (321.60 ± 5084.43 kcal/mol) was lower than the native ligand (569.25 ± 8997.10 kcal/mol) and gefitinib (623.87 ± 9862.73 kcal/mol), indicating weaker thermodynamic binding strength. This discrepancy arises as docking measures potential binding in a static state, while MM-PBSA reflects dynamic, realistic binding strength. These results suggest that vernoniosides A3 shows promising binding affinity but requires further optimization and validation for TNBC therapy.
In Silico Evaluation of Bisbenzylisoquinoline Compounds from Stephania sp. as EGFR Inhibitors for Triple-Negative Breast Cancer Amanda Triastuti Amanda; Denny Satria
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 02 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss02/677

Abstract

Triple-negative breast cancer (TNBC) represents a highly aggressive breast malignancy subtype characterized by constrained therapeutic alternatives and poor clinical outcomes. Since Epidermal Growth Factor Receptor (EGFR) is frequently overexpressed in TNBC, it remains a strategic molecular target for anticancer drug innovation. This study evaluates the inhibitory potential of bisbenzylisoquinoline (BBIQ) compounds from Stephania species against EGFR using an in silico framework. Molecular docking analysis revealed that isotrilobine possessed the highest binding affinity toward EGFR, achieving a docking score of -10.5 kcal/mol, indicating potent interactions within the active site. Molecular dynamics simulations over a 100 ns trajectory demonstrated that the EGFR-isotrilobine complex maintained structural stability throughout, as evidenced by RMSD values of 0.193-1.223 Å, RMSF of 1.5 ± 1.1 Å, radius of gyration (Rg) of 4.597-4.918 Å, and a solvent-accessible surface area (SASA) of 772.3 Ų. Hydrogen bond analysis identified an average of four stable interactions, reinforcing persistent ligand binding within the receptor pocket. Furthermore, MM-PBSA calculations yielded a binding free energy of 101.063 ± 127.26 kJ/mol, indicating energetically favorable binding. These findings suggest that isotrilobine and related BBIQ analogues exhibit significant potential as EGFR inhibitors for TNBC therapy, serving as viable candidates for further experimental validation and drug development.
The Immunomodulatory Activities of Saurauia vulcani Korth Leaves towards RAW 264.7 cell R Rosidah; Y Yuandani; Sri Suryani Widjaja; Muhammad Fauzan Lubis; Denny Satria
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1430

Abstract

Saurauia vulcani Korth is an actinidiaceae family plant that is used as traditional medicine by the community. Testing the immunomodulatory effect of Saurauia vulcani Korth extract was done in vitro on RAW cells line by stratified extraction using n-hexane, ethyl asetat, and ethanol solvents. Determination of nitric oxide levels by the griess method and inhibition of gene expression TNF-α, IL-6, COX-2, IL-1β, and iNOS by RT-PCR method. Saurauia vulcani Korth extract (NESVL, EAESVL, and EESVL) at a concentration of 25 ug / ml was able to reduce the value of nitric oxide in RAW cells 246.7 and was able to inhibit the expression of genes TNF-α, IL-6, COX-2, IL-1β, and iNOS. Where the density value of each band formed by 1.21 ± 0.005; 1.15 ± 0.005; 0.88 ± 0.003 on TNF-α, 1.23 ± 0.003; 1.64 ± 0.005; 0.55 ± 0.003 on IL-6, 0.38 ± 0.003; 0.55 ± 0.003; 0.38 ± 0.003 on COX-2, 0.18 ± 0.003; 0.04 ± 0.003; 0.03 ± 0.003 on IL-1β, and 0.30 ± 0.003; 0.41 ± 0.003; 0.11 ± 0.005 on iNOS.
Co-Authors Amanda Triastuti Amanda Aminah Dalimunthe Arga Lubis Avi Syafitri Cintya, Henni Effendy De Lux Putra, Effendy De Lux Gisela Agustin Habayahan Hanifah Sabrina Hariyadi Dharmawan Hariyadi Dharmawan Syahputra Hariyadi Dharmawan Syahputra Hariyadi Dharmawan Syahputra Hasibuan, Poppy Anjelisa Zaitun Henni Cintya Jansen Silalahi Kasta Gurning M. Fauzan Lubis Mahatir Muhammad Meiva Amelia Lubis Muhammad Fauzan Lubis Muhammad Fauzan Lubis Nasri N Nasri Nasri Nasri Nasri Olivia Avriyanti Hanafiah Panal Sitorus Rosidah, Rosidah Sri Suryani Widjaja Suryati Cintia Gultom Vera Estefania Kaban Yuandani Zulmai Rani