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All Journal HAYATI Journal of Biosciences Damianus Journal of Medicine The Indonesian Biomedical Journal Jurnal Medik Veteriner Acta Biochimica Indonesiana JISIP: Jurnal Ilmu Sosial dan Pendidikan Neurologico Spinale Medico Chirurgico Asian Australasian Neuro and Health Science Journal (AANHS-J) JSIP: Jurnal Studi Ilmu Pemerintahan The Indonesian Journal of Community and Occupational Medicine Salam (Islamic Economics Journal) Saraq Opat : Jurnal Administrasi Publik Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Wawan Mulyawan
Aviation Medicine Program, Department Of Community Medicine, Faculty Of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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Institutional Strengthening of Farmers' Groups in a Joint Empowerment Approach in Lelamase Village, Rasanae Timur District, Bima City Ardyansah, Ady; Mulyawan, Wawan; Hendra, Hendra; Adilansyah, Adilansyah
JISIP: Jurnal Ilmu Sosial dan Pendidikan Vol 10, No 1 (2026): JISIP (Jurnal Ilmu Sosial dan Pendidikan) (Januari)
Publisher : Lembaga Penelitian dan Pendidikan (LPP) Mandala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58258/jisip.v10i1.10098

Abstract

This study aims to analyze the empowerment processes and institutional strengthening of farmer groups (Gapoktan) in Lelamase Village, Rasanae Timur District, Bima City, through education, training, and farm management development programs. Using a qualitative case study approach, the research involved purposively and snowball-selected informants, including Gapoktan leaders, farmer group members, agricultural extension officers, and local government officials. Data were collected through in-depth interviews, direct observation, and document analysis, and were examined using the interactive Miles and Huberman model, with validity ensured through triangulation and member checking. The findings reveal that institutional strengthening through education and training is highly effective, particularly in improving managerial capacities, practical skills, and technical understanding of both group leaders and facilitators. Farm management development also operates optimally, reflected in improved economic scale, commercial orientation, cooperation with rural cooperatives (KUD), partnerships with relevant stakeholders, and enhanced analysis of local resource and market potential, as well as the diversification of supplementary economic activities. Overall, the synergy among extension services, government support, and farmer group initiatives has successfully enhanced institutional capacity, autonomy, professionalism, and the overall welfare of farmers. These results underscore that structured and continuous empowerment strategies significantly contribute to strengthening agricultural institutions at the local level.
Intermittent Exposure to Hypobaric Hypoxia Increases VEGF, HIF-1α, and Nrf-2 Expressions in Brain Tissue Wardaya Wardaya; Dewi Sukmawati; Nurhadi Ibrahim; Frans Ferdinal; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A. Jusman; Irawan Satriotomo; Wawan Mulyawan
The Indonesian Biomedical Journal Vol 17, No 2 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i2.3519

Abstract

BACKGROUND: Hypoxia inducible factor-1α (HIF-1α), nuclear factor erythroid 2-related factor 2 (Nrf-2), and vascular endothelial growth factor (VEGF), play a crucial role as neuroprotective factors. Currently, there is a lack of studies examining the biomolecular responses of the brain to intermittent hypoxia resulting from various pressures. This study was conducted to investigate the physiological responses, histopathological features, and cellular adaptive responses in the brains of rats that were intermittently exposed to hypobaric hypoxic conditions.METHODS: Thirty male Sprague Dawley rats were divided into six groups: a control group and five treatment groups exposed to hypobaric hypoxia. The treatment groups were placed in a hypobaric chamber simulating an altitude of 3,048 meters for 1 hour/day for 1, 7, 14, 21, and 28 days. After exposure, brain tissue was collected for histopathological analysis and protein quantification of HIF-1α, Nrf-2, cytoglobin (Cygb), neuroglobin (Ngb), VEGF, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT).RESULTS: In the brain, intermittent hypobaric hypoxia significantly increased HIF-1α expression (p=0.000) and its downstream proteins Cygb (p=0.000), and VEGF (p=0.001), with a peak at 14x IHH exposure compared to control. This was followed by a significant increase in Nrf-2 expression (p=0.000), SOD (p=0.000), Gpx (p=0.000), and CAT activity (p=0.000), indicating an adaptive antioxidant response. Conversely, MDA levels was decreased with prolonged exposure, suggesting reduced oxidative damage.CONCLUSION: IHH elevates HIF-1α, Nrf-2, and oxidative stress markers, triggering an adaptive antioxidant response in the rat’s brains.KEYWORDS: HIF-1α, intermittent hypobaric hypoxia, Nrf-2, oxidative stress
Hyperbaric Oxygen Therapy for Traumatic Brain Injury: A Review Of History, Development, Current Techniques, and Future Directions Wiwoho, Yudi Yuwono; Sadikin, Abdul Halim; Jusuf, Ahmad Aulia; Mulyawan, Wawan; Mudjihartini, Ninik; Ibrahim, Nurhadi; Jusman, Sri Widia A.; Sadikin, Mohamad
Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education Research Institute Vol. 9 No. - (2025): Proceedings Book of International Conference and Exhibition on The Indonesian M
Publisher : Writing Center IMERI FMUI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69951/proceedingsbookoficeonimeri.v9i-.321

Abstract

Hyperbaric oxygen therapy (HBOT) has gained increasing attention as a potential adjunctive treatment for traumatic brain injury (TBI) patients. This narrative review discusses the historical background, current preclinical and clinical studies, and explores its underlying mechanisms from biomolecular, histological, and clinical perspectives. HBOT promotes neural recovery by improving oxygenation, preserving mitochondrial integrity, enhancing neurotrophic support and synaptic connectivity, mitigating secondary injury pathways (including oxidative stress, inflammation, and apoptosis), and promoting angiogenesis and vascular stability. These mechanisms have demonstrated improvements of motor, cognitive, and memory functions both in preclinical and clinical studies, although outcomes and treatment protocols vary. However, challenges remain regarding optimal protocols, patient selection, and adverse effects. Further high-quality clinical trials are required to define the optimal HBOT regimen are required.
Efek proteksi hipoksia hipobarik intermiten pada otak tikus: Analisis konsentrasi Caspase-3, Neutrophin-3, karbonil dan SOD Ninik Mudjihartini; Defli Yuandika Ruso; Wawan Mulyawan; Atikah Chalida Barasila; Yulhasri
Bahasa Indonesia Vol 25 No 1 (2026): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v25i1.6554

Abstract

Introduction: The brain is the largest energy-consuming organ in the body and is highly vulnerable to damage under hypoxic conditions. However, certain periods of hypoxia can be tolerated and provide protective effects. This study aimed to investigate the protective effects of intermittent hypobaric hypoxia (IHH) on the expression of caspase-3, neurotrophin-3, carbonyl, and superoxide dismutase (SOD) levels in rat brain tissues. Methods: A total of 25 male Wistar rats were divided into five groups: a control group (normoxic conditions) and four treatment groups exposed to hypobaric hypoxia in a chamber simulating 25,000 feet altitude for 5 minutes at intervals of 7 days for each exposure. Group 1 was exposed to a one-time hypoxia condition (IHH 1), group 2 was exposed two times (IHH 2), group 3 was exposed three times (IHH 3), and group four were exposed four times (IHH 4). Results: Caspase-3 levels significantly decreased in the treatment groups compared to the control group (p<0.05), indicating a positive response to IHH. In contrast, neurotrophin-3 and carbonyl levels showed no significant changes across the groups, maintaining a stable trend. Additionally, a significant increase in SOD levels was observed between group 1 and group 3 (p<0.05), suggesting enhanced antioxidant defense with repeated IHH exposure. Conclusion: Intermittent hypobaric hypoxia can suggest a protective effect against hypoxic damage by suppressing caspase-3 expression. Repeated IHH exposure enhances antioxidant defense by elevating SOD levels. In contrast, neurotrophin-3 and carbonyl levels remain unchanged, suggesting these markers are unresponsive to short-term IHH.
Intermittent hypobaric hypoxia induces oxidative stress and vascular remodeling in rat pulmonary arteries Sri Nurbowo Ardi; Muhammad Arza Putra; Dewi Sukmawati; Puspita Eka Wuyung; Wawan Mulyawan; Wardaya; Wiwien Heru Wiyono
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.29-40

Abstract

Flight crews frequently experience intermittent hypobaric hypoxia (IHH), which may trigger oxidative stress and vascular remodeling in the pulmonary arteries. This study investigated the effects of IHH exposure on oxidative stress markers, tunica media thickness, and the expression of LR11 and HIF-1α proteins in rat pulmonary arteries using five groups of healthy male Sprague–Dawley rats (n = 5 per group). Twenty-five male Sprague–Dawley rats were divided into five groups: a control group and four treatment groups exposed to simulated altitudes of 25,000 ft (7,620 m) for 5 minutes per day over 1, 3, 7, or 14 days. Levels of HIF-1α and LR11 were measured using ELISA, oxidative stress markers such as γ-glutamyl-cysteinyl-glycine (GSH) and malondialdehyde (MDA) were analyzed spectrophotometrically, and tunica media thickness was assessed histologically. HIF-1α levels peaked after acute hypoxia exposure and declined with prolonged exposure, whereas LR11 levels progressively increased, peaking in the 14-day group. MDA levels, reflecting oxidative stress, were highest after 3 days of exposure, while GSH levels decreased in all hypoxia groups compared with the control. Histological analysis showed the greatest tunica media thickness in the 3-day exposure group, followed by gradual reductions. Although no statistically significant correlations were found between protein levels and tunica media thickness, similar directional trends suggest biologically relevant associations. Intermittent hypobaric hypoxia (IHH) induces dynamic changes in oxidative stress and vascular remodeling in rat pulmonary arteries. Acute hypoxia increases HIF-1α expression and oxidative stress, whereas repeated exposures appear to trigger compensatory adaptations, including LR11 upregulation and tunica media remodeling. These findings demonstrate measurable molecular and structural alterations underlying pulmonary vascular responses to repeated high-altitude exposure.
Analisis Biomarker Oksidatif dan Saturasi Oksigen Paru pada Tikus Hipoksia Hipobarik Intermiten Sri Nurbowo Ardi; Wiwien Heru Wiyono; Muhammad Arza Putra; Dewi Sukmawati; Puspita Eka Wuyung; Wawan Mulyawan; Wardaya Wardaya
Bahasa Indonesia Vol 25 No 2 (2026): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v25i2.7093

Abstract

Pendahuluan: Hipoksia hipobarik intermiten (IHH) dapat mengganggu homeostasis redoks dan menurunkan saturasi oksigen. Malondialdehid (MDA) mencerminkan peroksidasi lipid, sedangkan reduced glutathione (GSH) menggambarkan kapasitas antioksidan endogen. Penelitian ini mengevaluasi MDA dan GSH plasma serta saturasi oksigen perifer (SpO₂) setelah paparan IHH akut dan berulang pada tikus. Metode: Sebanyak 25 tikus jantan Sprague-Dawley dibagi acak ke dalam lima kelompok (n=5/kelompok): kontrol normobarik dan IHH 1×, 3×, 7×, atau 14×. IHH diberikan sekali sehari dalam ruang hipobarik setara 25.000 kaki (7.620 m) selama 5 menit. MDA plasma diukur dengan TBARS dan GSH dengan uji DTNB. SpO₂ dicatat segera setelah paparan menggunakan pulse oximetry untuk rodensia. Perbedaan antarkelompok dianalisis dengan ANOVA satu arah dan Tukey HSD. Hasil: GSH berbeda bermakna antarkelompok (F=30,022; p<0,001) dan lebih rendah pada seluruh kelompok IHH dibanding kontrol, dengan nilai terendah setelah 7 kali paparan. MDA juga berbeda bermakna (F=14,481; p<0,001), dengan nilai tertinggi setelah 3 kali paparan; IHH 3× lebih tinggi daripada kontrol (p<0,001) dan IHH 14× (p=0,007). SpO₂ lebih rendah pada seluruh kelompok IHH dibanding kontrol (p<0,001), dengan rerata terendah setelah 3 kali paparan (61,2±4,66%). Simpulan: IHH berkaitan dengan desaturasi oksigen dan perubahan biomarker stres oksidatif sirkulasi. Pola MDA dan GSH yang tidak linear konsisten dengan respons redoks yang bergantung waktu, tetapi belum membuktikan adaptasi fisiologis.
Co-Authors Adilansyah, Adilansyah Agus Sugiharto Ahmad Aulia Ani Retno Prijanti ARDYANSAH, ADY Atikah Chalida Barasila Defli Yuandika Ruso Dewi Sukmawati Fanny Septiani Farhan Fazlin Khuzaima Firdaus Firdaus Frans Ferdinal Frans Ferdinal Hendra Hendra Irawan Satriotomo Mohamad Sadikin Muhammad Arza Putra Ninik Mudjihartini Novi S. Hardiany Nurhadi Ibrahim Nurhadi Ibrahim Puspita Eka Wuyung Retno Wibawanti Rolian, Danu Sadikin, Abdul Halim Septelia I. Wanandi Sri Nurbowo Ardi Sri Widia A.Jusman Syaiful Ichwan Syarifah Dewi Tasrif Tasrif Wardaya Wardaya Wardaya Wardaya Wardaya Wiwien Heru Wiyono Wiwoho, Yudi Yuwono Yudi Yuwono Wiwoho Yulhasri  Dewi S. Soemarko  M.  Ilyas