Widodo Widodo
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Kamper Kampus IPB Darmaga, Bogor 16680, Indonesia

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Journal : Microbiology Indonesia

Fusarium Species Associated with Corm Rot of Taro in Bogor . WIDODO; . SUPRAMANA
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.153 KB) | DOI: 10.5454/mi.5.3.6

Abstract

In Bogor-West Java, corm rot disease of taro has devastated in many cropping areas and caused yield losses up to 70%. During preliminary studies, Fusarium species were constantly recovered when diseased materials, rotten corms and discolored petioles were incubated. The objectives of this study was to identify the Fusarium species associated with the corm rot disease in Bogor and determine their pathogenicity and hosts range. Samples were collected from 40 diseased corm samples taken in 9 sub-districts of Bogor. Two species, viz, Fusarium solani and F. oxysporum were identified based on the morphological characteristics. Among 40 Fusarium isolates recovered in this study, 70% were Fusarium solani and 30% were F. oxysporum.  F. solani could infect to all tested edible Araceae , while F. oxysporum was only pathogenic to Colocasia esculenta. Both species of Fusarium did not cause any symptoms when inoculated on selected ornamental and legume crops. These results gave the indication that F. solani was probably pathogenic only to edible Araceae, but further inoculation assay on living taro plants are necessary to carry out in order to clarify this result.
Selection of Yeasts Antagonists as Biocontrol Agent of Mango Fruit Rot caused by Botryodiplodia theobromae DWI SUGIPRIHATINI; SURYO WIYONO; . WIDODO
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.9 KB) | DOI: 10.5454/mi.5.4.2

Abstract

Fruit rot caused by Botryodiplodia theobromae is one of the most important post harvest disease of mango in Indonesia. Study on biological control on the disease is required to develop environmentally-sound control technology.  The research objectives were to study the potency of yeasts in controlling post harvest mango disease i.e. fruit rot caused by B. theobromae and mechanism involve in the biocontrol. Total yeast isolates used for screening were twenty one, four from collection of Plant Clinic of Institut Pertanian Bogor, and twenty one isolated from healthy mango skin. All of yeast isolates were characterized and identified using BIOLOG. Bioassay on antagonistic activity of yeasts against fruit rot, in-vitro dual culture test and chitinolytic activity were carried out.  Cryptococcus albidus var. aerius WSW1, Pichia guilliermondii K1, and Debaryomyces hansenii K12 were the three most effective antagonistic yeasts against B. theobromae with effectiveness of 70.83%, 45.83%, 37.50% respectively. In vitro bio-assay showed that C. albidus var. aerius WSW1, C. albidus WSW2, C. albidus K6, C. terreus YSW1, Candida edax OSW1, Candida edax K13, and Cryptococcus luteolus K2 had high antibiosis activity.  Biocontrol activity of tested yeasts against fruit rot of mango did not correlate to its antibiosis and chitinolytic activity