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Journal : Makara Journal of Science

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BIODEGRADASI FENANTREN OLEH BAKTERI LAUT Pseudomonas sp KalP3b22 ASAL KUMAI KALIMANTAN TENGAH Murniasih, Tutik; Yopi, Yopi; Budiawan, Budiawan
Makara Journal of Science Vol. 13, No. 1
Publisher : UI Scholars Hub

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The Biodegradation of Phenanthren by Marine Bacteria Pseudomonas sp KalP3b22 from Kumai Central Borneo. The study of the potential marine bacteria that degrade Polycyclic Aromatic Hydrocarbons (PAHs) was already done among several marine bacterium isolated from the contaminated sea water collected in Kumai Port area. The biodegradation rate of phenanthrene by selected bacteria Pseudomonas sp Kalp-3b22 was 59,5% after 29 days cultivation. The structural determination of degradation product was deduced as 1-naphtalenol.
OPTIMASI BIODEGRADABILITAS DAN UJI TOKSISITAS HASIL DEGRADASI SURFAKTAN LINEAR ALKILBENZENA SULFONAT (LAS) SEBAGAI BAHAN DETERJEN PEMBERSIH Budiawan, Budiawan; Fatisa, Yuni; Khairani, Neera
Makara Journal of Science Vol. 13, No. 2
Publisher : UI Scholars Hub

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Optimization of Biodegradability and Toxicity Testing of Degradation Product from Linear Alkyl Benzene Sulfonate (LAS) Surfactant as Cleaning Detergent Agent. Linear Alkyl Benzene Sulfonate (LAS) is a surfactant used in laundry detergent as cleaning agent and toxic to aquatic organisms. Results shows, with the LAS concentration used (20 ppm) in medium, adaptation time and Acinetobacter sp. growth has shown better biodegradation ability than three other bacteria used Pseudomonas putida, Pseudomonas fluorescence, Bacillaria spp. Thus, Acinetobacter sp is used further for biodegradation process of LAS. Based on its biodegradation half-life using Acinetobacter sp, and with mixed culture (± 52.32% and ± 46.82% respectively) could be achieved in 4 (four) days, LAS could be categorized as a biodegradable compound. The toxicity assay is based on tetrazolium dye reduction with Rhizobium meliloti as indicator organism. LAS is more toxic than its intermediate product from biodegradation, with IC50 = 34.35 ppm, and the intermediate product, Ac and Cm, has IC50 = 446.19 ppm and 111.28 ppm respectively. Identification of intermediate products using IR and LC-MS analysis shows that the degradation product contains chemicals compounds with functional group as follows: benzene, benzoic acid, hydroxyl, and aliphatic carbons with large molecule weight. Until its half-time degradation time, LAS biodegradation process only occurs at the aliphatic carbon chain, and have not yet reached the stage of aromatic ring opening.
Co-Authors Abidah R Delu Azizah, Dwi Waliyatul Baiq Sri Handayani Cecep Agus Dara, Chenda Dwi Nopiyadi Faruk HT Heny Suseno Imam Bachtiar Indira Irawati, Indira Indirawati, Surachmi Intan Cahaya Dani, Intan Cahaya Khairani, Neera Laksono, PM Lalu Japa, Lalu Mawaddah, Usratul Mochamad Guntur Narwaya, St.Tri Guntur Puspitaningsih, Indah Putra, Indra Dwi Rahman, Wira Y Rith, Vicheka Saputra, Angga Adi Sholeha, Herisa Hardiyanti Sopiyani, Nuzula Sumanti Sumanti, Sumanti Syamsul Bahri Tutik Murniasih YOPI YOPI Yuni Fatisa