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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Current Biochemistry Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Greensphere: Journal of Environmental Chemistry Prosiding Seminar Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Anto Budiharjo
Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : SAINS DAN MATEMATIKA

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The Antioxidant Growth and Potency of Yeast Rhodosporidium paludigenum DUCC Y-007 on Different Mediums Kusdiyantini, Endang; Budiharjo, Anto
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 4 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

The antioxidant growth and potency of yeast Rhodosporidium paludigenum DUCC Y-007 have been studied on two treatment mediums. The yeast could grow on two treatment mediums and the dry weight results obtained during the incubation period of 120 hours were 7.17 g/L and 7.33 g/L. The concentration of reducing sugars in stabilized medium were 3.14 g/L and 3.30 g/L at 72-120 hours incubation time respectively. There were differences in pH changes during incubation time which at YPG medium, it tended to increase whereas at semi synthetic medium, the pH tended to decline. Total carotenoid on YPG medium was 50.13 µg/g cell and on the semi-synthetic medium was 197.50 µg/g cell. Antioxidant activity measured by DPPH reagent showed the results at YPG medium was 50% and at semi-synthetic medium was 61%.
Deteksi Jenis Padi Indica dan Japonica Padi Gogo Rancah Beras Merah Varietas Slegreng dan Mandel Berbasis Fragmen ORF100 dan ORF29 Haryanti, Wahyu Dewi U.; Kusumaningrum, Hermin P.; Budiharjo, Anto
JURNAL SAINS DAN MATEMATIKA Volume 21 Issue 4 Year 2013
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Deteksi fragmen Open Reading Frame (ORF)100 dan ORF29 telah dilakukan pada dua jenis padi gogo rancah beras merah yaitu varietas Slegreng dan Mandel. Metode penelitian dilakukan dengan cara isolasi DNA kloroplas padi diikuti dengan amplifikasi PCR menggunakan optimasi suhu annealing pada tiga suhu yang berbeda, yaitu 53°C, 55°C, dan 56°C. Selanjutnya hasil amplifikasi divisualisasikan menggunakan elektroforesis pada gel agarose 1%. Suhu annealing 53°C merupakan suhu yang optimal untuk memperlihatkan fragmen ORF100 dan ORF29. Hasil penelitian menunjukkan bahwa fragmen ORF100 dan ORF29 dapat teramplifikasi pada padi Slegreng dan mandel, sehingga kedua jenis padi tersebut menunjukkan kecenderungan subspesies japonica. Perbedaan ketebalan dan ukuran pita tidak mempengaruhi keberadaan fragmen ORF100 dan ORF29 sebagai penanda untuk mengidentifikasi tipe indica atau japonica. Pemanfaatan marka molekuler fragmen ORF100 dan ORF29 dalam mendeteksi jenis padi diharapkan dapat melengkapi hasil karakterisasi dan pengelompokkan varietas padi berdasar karakter morfologi dan fisiologi.   Keywords: chloroplast DNA, ORF100, ORF29, indica-japonica differentiation
Co-Authors AB SUSANTO Abhinaya, Danendra Daryl Ade Fajrian Adila Nawan Hasrimi Agung Suprihadi Agung Suprihadi Ahmad Thontowi Anggoro, Naufal Sebastian Aninditia Sabdaningsih Ariani, Nur Frida Asgasatya, Syahid Fattah Barri Pratama Budi Raharjo Bunga Fajriani CATUR RIANI Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Deverina Mergypta Dian Wahyu Kemalaputri Dudi Hardianto, Dudi Dyah Ayu Riani Dyah Wulandari Edwin Setiawan Elfira, Yolanda Elke Gildantia Elvi Yetti Endang Kusdiyantini Endang Kusdiyantini Fahmi, Maya Fitriana Ilul Fauziah Citra Rahmawati Fitri Arum Sasi, Fitri Arum Fitria, Lutfiah Rahmadini Florentina Kusmiyati HARTAJANIE, LAKSMI Hasrimi, Adila Nawan Heni Wijayanti, Heni Hermanto, Michael Einstein Hermin P. Kusumaningrum Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Husna, Faza Laili IS HELIANTI Khoirul Huda Kristina Kristina Kurniawati, Mufida Budi Kusumaningrum, Hermin Laila Nur Faizah Lasria Pardede Lindayani, Lindayani Luthfy AN M Masfuroh Maerani Sumarno Mahmudah, Hawari Rosdiana Maria, Atina Maulana, Anand Reyna Mawarni, Risa Arum Maya Fitriana Ilul Fahmi MG Isworo Rukmi Mia Tri Wardani Monalita, Ramadhebi Muhammad Adib Mubarok Muhammad Mulyadi, Muhammad Muhammad Zainuri Norma Sainstika Pangestu Novita Rahmawati, Novita Nur Endah Wahyuningsih Nurhayati Nurhayati Nurlita Abdulgani Octavia Dewi Christiningrum Olivia Nisa Mumtianah Pardede, Lasria Prayogo, Fitra Adi Rahayu, Annisa Rizky Rahmah Putri Sunarno Ratna Cempaka Lingga Rejeki Siti Ferniah Risky Panji Nugroho Risma Wiharyanti Safirah, Dearesty Saniha Adini, Saniha Sayono Setyo Nugroho Shabrina, Jauhara Siti Nur Jannah Siti Nur Jannah Siu S.S Langden Sonny Abdi Setiyawan, Sonny Abdi Soowanayan, Chumporn Sri Pujiyanto Suetrong, Satinee Sumarno, Maerani Suprihadi Susiana Purwantisari Teuku Tajuddin Thu'ti Alawiyyah Tony Hadibarata, Tony w wijanarka Wahyu Dewi U. Haryanti Wien Kusharyoto Wijanarka Wijanarka Yason Lukman Sudjito, Yason Lukman Yohanes Chandrawijaya Yopi Yopi Yuliandini Pangestika