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Penapisan aktinobakteria rhizosfer padi sebagai agens pengendali hayati Xanthomonas oryzae pv. oryzae pathogen penyebab penyakit hawar daun bakteri Muhammad Fadil; Yulmira Yanti; Ujang Khairul
Jurnal AGRO Vol 10, No 1 (2023)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/19798

Abstract

Bacterial leaf blight caused by X.o. pv. oryzae is an important disease of rice plants. Actinobacteria has potential as biological agents to control X.o. pv. oryzae because it has the ability to produce bioactive compounds. This study aimed to select actinobacteria isolates that can suppress the development of X.o. pv. oryzae and has the potential to stimulate the growth of rice plants in-planta, as well as to determine the ability of actinobacteria in producing enzymes that inhibit the development of X. oryzae pv. oryzae. The research consisted of four stages, namely: isolation, selection, characterization, and potential inhibition of actinobacterial isolates. A total of 30 isolates were successfully isolated from the rhizosphere of rice plants in three districts of West Sumatra, and as many as 25 isolates were successfully selected based on biosafety tests. The results of the in-planta test showed that 10 isolates had the ability to increase the growth and suppresed the development of bacterial leaf blight. The results of the antagonist test showed that 5 isolates inhibited of X.o. pv. oryzae by 11.66-29.66%. Five isolates were selected, namely: APRD 3I211, APRD 1I122APRP 2S121, APRP 1I121, APRP 3I212 wich capable of produce protease enzymes, cellulases, amylase, and secondary metabolites.ABSTRAK Penyakit hawar daun bakteri yang disebabkan oleh Xanthomonas oryzae. pv. oryzae merupakan penyakit penting tanaman padi. Aktinobakteria memiliki potensi sebagai agens hayati untuk mengendalikan X. oryzae. pv. oryzae karena memiliki kemampuan dalam menghasilkan senyawa bioaktif. Penelitian ini bertujuan untuk menyeleksi isolat aktinobakteria yang dapat menekan perkembangan X. oryzae. pv. oryzae dan memiliki potensi dalam memacu pertumbuhan tanaman padi secara in-planta, serta mengetahui kemampuan aktinobakteria dalam menghasilkan enzim penghambat perkembangan X. oryzae. pv. oryzae. Penelitian terdiri dari empat tahap, yaitu: isolasi, seleksi, karakterisasi, dan potensi daya hambat isolat aktinobakteria. Sebanyak 30 isolat berhasil diisolasi dari rizosfer tanaman padi di tiga Kabupaten Sumatera Barat, dan sebanyak 25 isolat berhasil diseleksi berdasarkan uji keamanan hayati. Hasil uji in-planta menunjukkan 10 isolat memiliki kemampuan dalam meningkatkan pertumbuhan dan menekan perkembangan hawar daun bakteri. Hasil uji antagonis menunjukkan 5 isolat menghasilkan penghambatan terhadap X. oryzae. pv. oryzae sebesar 11,66-29,66%. Lima isolat terpilih yaitu: APRD 3I211, APRD 1I122, APRP 2S121, APRP 1I121, APRP 3I212 terbukti mampu menghasilkan enzim protease, selulase, amilase, metabolit sekunder.
IDENTIFIKASI RAYAP PADA PERKEBUNAN KELAPA SAWIT SEPANJANG ALIRAN SUNGAI BATANG HARI MELEWATI KECAMATAN PULAU PUNJUNG KABUPATEN DHARMASRAYA Sri Heriza; Ridho Herdi; Ujang Khairul
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 9, No 2 (2024): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v9i2.15794

Abstract

ABSTRAK Tanaman kelapa sawit di Indonesia memiliki arti penting bagi pembangunan perkebunan nasional. Provinsi Sumatera Barat termasuk salah satu provinsi penghasil kelapa sawit di Indonesia. Kabupaten Dharmasraya termasuk salah satu kabupaten penghasil kelapa sawit di Provinsi Sumatera Barat dan tergolong daerah yang invasif dalam meningkatkan luas perkebunan kelapa sawit. Kabupaten Dharmasraya memiliki penduduk dengan mayoritas pekerja sebagai petani tanaman kelapa sawit. Sehubungan dengan hasil produksi tanaman kelapa sawit tersebut, Kabupaten Dharmasraya yang daerahnya dilintasi oleh Sungai Batang Hari sebahagian besar menanam kelapa sawit disepanjang aliran sungai tersebut. Kendala dalam usaha perkebunan kelapa sawit adalah serangan hama. Salah satu hama penting pada perkebunan kelapa sawit rakyat di sepanjang Sungai Batang Hari adalah rayap. Rayap merupakan serangga sosial yang termasuk kedalam ordo Isoptera yang pada dasarnya merupakan serangga daerah tropika dan subtropika. Kondisi lingkungan dan kelembapan juga mempengaruhi keragaman rayap pada suatu habitat. Penelitian ini bertujuan untuk mengidentifikasi jenis rayap pada perkebunan kelapa sawit rakyat di sepanjang aliran Sungai Batang Hari melewati Kecamatan Pulau Punjung Kabupaten Dharmasraya. Identifikasi dilakukan dengan cara menyusuri aliran Sungai Batang Hari di Kecamatan Pulau Punjung Kabupaten Dharmasraya, dengan mengamati sarang rayap sekitar pertanaman kelapa sawit secara langsung yang berada di pinggir Sungai Batang Hari. Sarang rayap yang ditemukan selanjutnya dilakukan pembongkaran untuk kemudian diambil sampel rayap yang akan diidentifikasi. Sampel rayap yang dikoleksi adalah rayap kasta prajurit dan pekerja, yang diawetkan menggunakan alkohol 70%. Hasil penelitian menunjukkan hanya satu jenis rayap yang ditemukan pada perkebunan kelapa sawit rakyat di sepanjang aliran Sungai Batang Hari yaitu spesies Macrotermes gilvus.
Antagonistic Test of Endophytic Bacteria Against Fusarium oxysporum f.sp. cepae Causes of Moler Disease on Shallots Wihayyu, Afri; Resti, Zurai; Sulyanti, Eri; Darnetty, Darnetty; Khairul, Ujang
CROPSAVER Vol 7, No 1 (2024)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v7i1.54351

Abstract

Fusarium oxysporum f.sp. cepae (FOCe) is a pathogenic fungus causing moler disease in shallots. One method of controlling this disease is by using endophytic bacteria. Endophytic bacteria are bacteria that live in plant tissues without causing disease symptoms. This research aims to obtain the most effective endophytic bacteria in inhibiting the growth of the pathogenic fungus FOCe. The study employs a Completely Randomized Design (CRD) consisting of 8 treatments with 3 replications each. The treatments include Bacillus sp. HI, Bacillus sp. SJI, Bacillus cereus P14, Bacillus cereus Se07, Bacillus subtilis, Serratia marcescens JB1E3, Serratia marcescens ULG1E4, and control. Tests conducted include the antibiosis test of endophytic bacterial cells using the dual culture method and the secondary metabolite test using media poisoning method. The observed parameters include inhibition zone, effectiveness of secondary metabolites, fresh weight, and dry weight of the FOCe fungus. All endophytic bacteria were capable of inhibiting the growth of FOCe. The most effective endophytic bacteria in inhibiting the growth of FOCe were found to be Serratia marcescens ULG1E4 and Serratia marcescens JB1E3. In the antibiosis test of endophytic bacterial cells, Serratia marcescens ULG1E4 exhibited an inhibition zone of 64.44%, while Serratia marcescens JB1E3 showed an inhibition zone of 61.11%. In the secondary metabolite compound test, the effectiveness values for Serratia marcescens ULG1E4 and Serratia marcescens JB1E3 were 95.31% and 95.03%, respectively.
PEMBERDAYAAN KELOMPOK TANI MELALUI PELATIHAN PENGENDALIAN HAMA DAN PENYAKIT TANAMAN KAKAO DI KANAGARIAN CAMPAGO KABUPATEN PADANG PARIAMAN Ujang Khairul; Trizelia Trizelia; Reflin Reflin
BULETIN ILMIAH NAGARI MEMBANGUN Vol. 1 No. 4 (2018)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bina.v1i4.41

Abstract

Training on methods of pests and diseases management in cocoa plants has been conducted in the Nagari Campago, Kecamatan V Koto Kampung Padang Pariaman. The aims of this activity was to empower farmer groups through increasing the knowledge of cocoa farmers about cultivation and pests and diseases management attacked of cocoa plant, this activity consists of socialization, counseling and field practice. Evaluations carried out before and after training related to farmers' knowledge about cultivation and pests and diseases in cocoa. The results of the activity show that: (a) the level of pests and diseases in cocoa plantations in this area is quite high (55% dan 65%), (b) farmers have not good cultivated on cocoa, (c) farmers do not understand abaut pests and diseases that attack their cocoa and do not understand the methods for controlling pests and diseases. This activity has increased farmers' knowledge about the cultivation and management of pests and diseases in cocoa plants.
Endophytic Bacteria Consortia as Biocontrol Against Brown Spot Disease (Helminthosporium oryzae) and Growth Promotion in Rice Sulyanti, Eri; Resti, Zurai; Rahma, Haliatur; Darnetty, Darnetty; Khairul, Ujang; Nurhaliza, Nurhaliza; Oktaviani, Silvia
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 9 No. 1 (2025): June 2025
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.9.1.1-13.2025

Abstract

The brown spot disease (BSD) caused by Helminthosporium oryzae in rice can lead to yield losses of up to 45 %. Using endophytic bacterial consortia as a biological agent is one of the most potential control methods for resisting the disease. This study aimed to obtain an endophytic bacterial consortium with the best efficacy in controlling BSD and its ability to enhance rice plant growth. Statistical analysis in this study was made using Analysis of Variance (ANOVA) with Duncan’s Multiple Range Test (DMRT) in a randomized experimental design comprising five treatments with five replications. The treatments included: A (Serratia marcescens ULG1E4 + S. marcescens JB1E3), B (S. marcescens ULG1E4 + S. marcescens JB1E3 + Serratia marcescens JB1E2), C (Bacillus sp. SJI + Serratia marcescens ULG1E4), D (positive control), and E (negative control). Testing was conducted in planta, with the introduction of the bacterial consortium (10⁸ cells/mL) via seed and root soaking methods. Subsequently, the leaves of 30-day-old rice plants were inoculated with H. oryzae by spraying a conidial suspension of 10⁶ conidia/ml. Parameters observed included disease progression (incubation period, incidence, and severity), seedling growth metrics (height, leaf number, root length, fresh and dry biomass), and overall plant development (plant height, leaf number, and tiller count). The results demonstrated that the consortium of Serratia marcescens ULG1E4, Serratia marcescens JB1E3, and Serratia marcescens JB1E2 effectively controlled BSD, achieving a 53.69% reduction in disease severity and promoted rice plant growth, evidenced by a 30.63% increase in leaf number
Tingkat Kesadaran Lingkungan Mahasiswa sebagai Kontribusi terhadap Tujuan Pembangunan Berkelanjutan (SDGs) Edwin, Tivany; Khairul, Ujang; Nur, Ansiha; Mardatillah, Regina; Arif Satria, Muhammad
Dampak Vol. 22 No. 2 (2025)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/dampak.22.2.96-103.2025

Abstract

University students are key agents in promoting environmental sustainability, making it essential to assess their level of knowledge, attitudes, and actions regarding environmental awareness. This study aims to evaluate environmental awareness among university students through a structured questionnaire survey. The instrument was distributed to 71 students across different academic levels at a university in West Sumatra Province. The results indicate that while most students possess a basic understanding of environmental issues, their ability to analyze, synthesize, and evaluate such issues remains limited. In terms of attitudes, students generally demonstrate positive dispositions, although further development is needed to foster active participation and social responsibility. Regarding environmentally conscious actions, students have not yet consistently adopted sustainable behaviors, particularly at the level of habitual and innovative practices. Key behaviors that require improvement include waste separation, refusal to use plastic bags, the use of reusable food containers, and recycling practices. These findings highlight the need for more practical and reflective environmental education at the higher education level. Keyword: university students, knowledge, attitude, action, environment
Community Empowerment Strategy for the Development of Aren Sugar Production Center in Koto Malintang, Lake Maninjau, West Sumatra Anwar, Aswaldi; Utami, Ami Sukma; Wisnubroto, Muhammad Parikesit; Adrinal, Adrinal; Khairul, Ujang; Pratama, Rafdi; Adib, Muhammad; Prasetyo, Rival; Susila, Rahma; Itra, Novi Rahmat
Buletin Dharmas Andalas Vol. 2 No. 2 (2025): Buletin Dharmas Andalas
Publisher : Departemen Budidaya Tanaman Perkebunan, Fakultas Pertanian, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bda.v2i2.43

Abstract

This community service program was implemented in Koto Malintang, West Sumatra, to empower farmer groups through the development of an Aren sugar (Arenga pinnata) production center. The activities consisted of pre-surveys, focus group discussions (FGDs), socialization workshops, participatory planning, and initial demonstrations of intercropping practices. A key component of the program was the financial feasibility analysis, designed to provide farmers and stakeholders with evidence-based tools for decision-making. The analysis was conducted using production costs and revenues from the research location, with a 15-year horizon and a 10% discount rate. Two cultivation scenarios were evaluated: (1) Aren intercropped with seasonal crops and (2) Aren intercropped with robusta coffee. Each scenario considered two product alternatives: molded sugar (gula cetak) and palm sugar crystals (gula semut). The results revealed that molded sugar offered only marginal feasibility, with Net Present Values (NPV) of Rp 28–35 million/ha, Internal Rates of Return (IRR) of 12–15%, Benefit-Cost (B/C) ratios around 1.1, and payback periods of 7–8 years. In contrast, palm sugar crystals showed strong financial viability, with NPVs of Rp 298–330 million/ha, IRRs of 38–45%, B/C ratios of 2.4–2.6, and payback periods of only 4–5 years. Sensitivity analysis confirmed robustness across discount rates of 8–12% and price variations between Rp 40,000–60,000/kg. These findings suggest that palm sugar crystals should be prioritized as the flagship product for Koto Malintang’s Aren sugar industry, supported by institutional strengthening, diversification, and modern marketing to ensure sustainability.
Biologi Penghisap Buah Helopeltis sp. (Hemiptera: Miridae) pada Buah Kakao dan Mentimun Nelly, Novri; Khairul, Ujang
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 1 No. 2 (2017): Desember 2017
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.1.2.62-67.2017

Abstract

This research purposed to study biology of Helopeltis sp. on cocoa and cucumber fruits. Research was done in insect bioecology laboratory using direct observation with 10 replications for each fruit. Parameter observed were copulation (hours), preoviposition time (days), number of eggs, nymphals, and adult periods (days), Oviposition time (days), and post oviposition time (days). The data obtained were analyzed descriptively and compared with t-test. Result of this research showed that Helopeltis sp. could thrive in cacao and cucumber fruits. Adult of Helopeltis sp. longevity in cacao fruits was 16 days and in cucumber fruits was 13 days. The number of eggs product average 10-10.9 eggs/female.
Jenis dan Tingkat Serangan Hama Utama Tanaman Ubi Kayu (Manihot esculenta Crantz) di Kabupaten Lima Puluh Kota: english Hidrayani, Hidrayani; Khairul, Ujang; Ratib, Fadilla; Ikhsan, Zahlul
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 3 No. 2 (2019): December 2019
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.3.2.85-92.2019

Abstract

Pests are one of constrains in cassava production. The research was done in cassava plantation in Lima Puluh Kota with the objectives were to determine the species of major pests of cassava and their damages. A survey was conducted in five subdistricts, Akabiluru, Guguak, Harau, Lareh Sago Halaban and Situjuah Limo Nagari. Twenty sampled plants were taken in 400 m2 plot laying in the middle of 0,5 hectar cassava plantation, 4-5 months. Four species of pests were found to attack cassava plants in Lima Puluh i.e: Paracoccus marginatus, Pseudococcus jackbeardsleyi, and Ferrisia virgata, whitefly, Aleurodicus dispersus. The three mealybugs caused percentage of plant attacked 85.5 %, percentage of leaf plant attacked 8.61 %, pest population 52.29 individual per plant with damage intensity was high. Whitefly caused percentage of plant attacked 12%, percentage of leaf plants attacked 0.77%, pest population 1.51 individual per plant.