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SINTESIS DAN KARAKTERISASI HIDROKSIAPATIT DARI TULANG SAPI (Bos taurus) MENGGUNAKAN TEKNIK KALSINASI SYNTHESIS AND CHARACTERIZATION OF HYDROXYAPATITE FROM COW BONES (Bos Taurus) USING CALCINATION TECHNIQUES Fifi Afifah; Sari Edi Cahyaningrum
Unesa Journal of Chemistry Vol 9 No 3 (2020)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.071 KB) | DOI: 10.26740/ujc.v9n3.p189-196

Abstract

Tulang sapi memiliki kandungan hidroksiapatit cukup tinggi, sehingga berpotensi sebagai prekursor material dalam sintesis hidroksiapatit. Tulang sapi memiliki komposisi anorganik yang terdiri dari 93% hidroksiapatit dan 7% β-TCP. Hidroksiapatit merupakan biomaterial dengan struktur heksagonal yang dapat dimanfaatkan sebagai implan tulang. Penelitian ini bertujuan untuk mengetahui hasil karakterisasi secara kimia (gugus fungsional dan fasa) maupun fisika (kristalinitas) sintesis hidroksiapatit dari tulang sapi. Tulang sapi dipreparasi dengan cara direbus, dicuci dengan aquades, lalu direndam dengan larutan H2O2 selama 5 jam sehingga dihasilkan tulang sapi bebas lemak berwarna putih. Hidroksiapatit diperoleh dari hasil kalsinasi tulang sapi menggunakan furnace dengan suhu 900⁰C selama 6 jam. Kalsinasi dilakukan dengan tujuan untuk mendapatkan hidroksiapatit dari tulang sapi dan untuk menghilangkan senyawa organik yang tidak dibutuhkan dalam tulang manusia. Rendemen yang dihasilkan pada proses kalsinasi sebesar 83,41%. Hidroksiapatit yang dihasilkan dikarakterisasi kimia dengan instrumen FT-IR menunjukkan adanya gugus fungsi OH-, PO43-, dan CO32-, dimana gugus-gugus tersebut merupakan ciri yang dimiliki oleh hidroksiapatit. Pada instrumen XRD menunjukkan adanya fasa hidroksiapatit dan fasa apatit karbonat. Fasa apatit karbonat tersebut tidak membahayakan tulang manusia disebabkan tulang manusia juga terdiri atas komposisi anorganik karbonat. Hidroksiapatit dikarakterisasi fisika menunjukkan derajat kristalinitas sebesar 95%. Hidroksiapatit memiliki kristalinitas yang tinggi karena memiliki struktur yang rapat dan pori yang kecil.Kata kunci : Tulang sapi, hidroksiapatit, kalsinasi Cow bone has a high hydroxyapatite composition, so it can be determined as a preliminary material in the synthesis of hydroxyapatite. Cow bone has an inorganic composition consisting of 93% hydroxyapatite and 7% (β-TCP). Hydroxyapatite is a biomaterial with a hexagonal structure that can be utilized as a bone implant. This research aims to study the results of chemical characterization (functional groups and phases) and physics (crystallinity) of hydroxyapatite synthesis from cow bones. Cow bones are prepared by boiling, replaced with distilled water, then soaked with a solution of H2O2 for 5 hours so that the resulting cow bone is free of white fat. Hydroxyapatite is obtained from the calcination of cow bones using a furnace at 900⁰C for 6 hours. Calcination is done to get hydroxyapatite from cow bones and to eliminate organic compositions that are not needed in human bones. The yield produced in the calcination process is 83.41%. Hydroxyapatite produced was chemically characterized by FT-IR instruments showing the presence of OH-, PO43-, and CO32- functional groups, whereas these groups were by the characteristics provided by hydroxyapatite. On the XRD intruments shows the presence of the hydroxyapatite phase and the apatite carbonate phase. This carbonate apatite phase does not contain human bones because human bones also consist of an inorganic carbonate composition. Hydroxyapatite characterized by physics shows a degree of crystallinity of 95%.Key words: Cow bone, hydroxyapatite, calcination
UJI DEGRADASI TERMAL PADA KOMPOSIT HIDROKSIAPATIT-KITOSANKOLAGEN SEBAGAI BONEGRAFT Maya Dany Puspitasari; Sari Edi Cahyaningrum
Unesa Journal of Chemistry Vol 10 No 1 (2021)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.442 KB) | DOI: 10.26740/ujc.v10n1.p12-15

Abstract

Abstrak. Komposit hidroksiapatit-kitosan-kolagen (HAp-kit-kol) merupakan biomaterial sintetik yang berfungsi dalam proses penyembuhan tulang. Komposit disintesis dengan metode ex-situ dan dibuat menjadi material berpori dengan metode freeze-drying. Komposit dikarakterisasi dengan TGA menghasilkan perubahan massa yang drastis pada suhu 230-510 oC sebesar 13,9% menandakan terjadinya dekomposisi material organik dalam komposit dan perubahan massa pada suhu 510-900 oC sebesar 4,2% menandakan terjadinya proses dekomposisi dan kristalisasi hidroksiapatit.Kata kunci: Komposit HAp-kitosan-kolagen, ex-situ, TGA. Abstract. Hydroxyapatite-chitosan-collagen (HAp-chit-coll) composites are synthetic biomaterials that function in the process of bone healing. Composites are synthesized by the ex-situ method and made into porous material by the freeze-drying method. Composites characterized by TGA produce a drastic mass change at a temperature of 230-510oC of 13.9% indicating the decomposition of organic material in the composite and a change in mass at a temperature of 510-900oC of 4.2% indicating the decomposition and crystallization of hydroxyapatite.Keywords: Composite HAp-chitosan-collagen, ex-situ, TGA.
Karakterisasi dan Uji Efektivitas Sediaan Gel Aloe vera Kombinasi Kitosan sebagai Antibakteri Staphylococcus aureus Sari Edi Cahyaningrum; Perintis Gita Susanti
Unesa Journal of Chemistry Vol 11 No 1 (2022)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.783 KB) | DOI: 10.26740/ujc.v11n1.p26-33

Abstract

The study about development synthesis gel of Aloe vera combination chitosan as Staphylococcus aureus antibacterial has been done. This study has the aim to determine the effect of composition comparison of Aloe vera with chitosan on the characterization and effectiveness of Staphylococcus aureus antibacterial as a candidate for wound healing. The synthesis the gel of Aloe vera combination chitosan was made by mixing of Aloe vera and chitosan solution with weight variated 2:1; 1:1; and 1:2 (AvK1, AvK2, and AvK3), and addition of Na-CMC, glycerin, and propylene glycol, which the gel was neutralized until it reached pH 6,5–7,0. The gel preparation were characterized by organoleptic, measuring viscosity, functional groups identification, and effectiveness test of Staphylococcus aureus antibacterial by diffusion method. The results were shown based organoleptic observations by color, smell, and texture. The lowest viscosity measurement characterization was showed by AvK3 gel, which was 13.0412 mPa.s. The result functional groups identification of all gel preparations was indicated the presence of O–H and N–H stretching vibrations, C=O stretching vibrations, C–H bending vibrations, and C–O stretching vibrations. Staphylococcus aureus antibacterial test from gel of Aloe vera combination chitosan with various variations were shown effective result, which the strongest was shown by AvK1 gel with an inhibition zone of 26 mm. Key words: chitosan, Aloe vera, antibacterial, Staphylococcus aureus
ADSORPSI ION LOGAM Zn (II) DAN Cu(II) PADA KITOSAN NANO BEAD DARI CANGKANG UDANG WINDU(Penaus monodon) (AdsorptionZn(II) and Cu(II) Ions on Chitosan Nanobeads From Shrimp Waste (Penaus monodon) Sari Edi Cahyaningrum; Narsito Narsito; Sri Juari Santoso; Rudiana Agustini
Jurnal Manusia dan Lingkungan Vol 18, No 3 (2011): November
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18442

Abstract

ABSTRAKTelah dibuat dua macam adsorben kitosan serbuk dan kitosan bead dari cangkang udang windu (Penaus monodon) sebagai bahan dasar, untuk adsorpsi ion logam Zn(II) dan Cu(II). Beberapa parameter adsorpsi seperti pH, laju adsorpsi, dan kapasitas adsorpsi dipelajari. Model kinetika adsorpsi orde satu dan orde 2 digunakan sebagai dasar untuk mengestimasi laju adsorpsi, sedangkan model isoterm adsorpsi Langmuir dan Freundlich digunakan untuk menetapkan kapasitas adsorpsi. Hasil penelitian menunjukkan bahwa adsorpsi ion logam Zn(II) dan Cu(II) pada kitosan serbuk dan kitosan bead mempunyai pH optimum yang sama. Laju adsorpsi ion logam Zn(II) dan Cu(II) pada kitosan bead secara signifikan lebih cepat dibanding pada kitosan serbuk. Modifikasi adsorben dengan proses swelling dapat meningkatkan kapasitas adsorpsi ion logam Zn(II) dan Cu(II) pada kitosan.ABSTRACTTwo typies of adsorbent have been  made, i.e: chitosan and chitosan bead using shell of shrimp (Penaus monodon ) as raw materials, for Zn(II) and Cu(II) metal ions adsorption. Adsorption parameters such as, pH, the adsorption rate, and the capacity of adsorption were determined. The adsorption kinetic  1st and 2nd order models  were applied in the estimation of the adsorption rate, whereas the isotherm adsorption Langmuir and Freundlich model were employed for   determination of the capacity of  adsorption.The results showed that adsorption of Zn(II) and Cu(II) metal ions on chitosan and chitosan bead have the same of optimum pH. The adsorption rate of Zn(II) and Cu(II) metal ions on chitosan bead significant faster than on chitosan. Modification with swelling process enhanced the adsorption capacity Zn(II) and Cu(II)  on chitosan. 
PREPARASI DAN KARAKTERISASI NANO KOMPOSIT KITOSAN-SILIKA DAN KITOSAN-SILIKA TITANIA (Preparation and Characterisation of Chitosan-Silica and Chitosan Silica Titania Nano Composites) Dina Kartika Maharani; Sari Edi Cahyaningrum; Amaria Amaria; Rusmini Rusmini
Jurnal Manusia dan Lingkungan Vol 19, No 1 (2012): Maret
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18452

Abstract

ABSTRAKTelah dibuat komposit kitosan silika dan kitosan silika titania dari larutan kitosan dan sol silika serta sol silika titania untuk aplikasi bahan antibakteri. Kitosan dibuat melalui proses deproteinasi, demineralisasi dan deasetilasi kitin dari cangkang kepiting. Sol silika dan silika titania dibuat dengan metode sol-gel. Komposit kitosan silika dan kitosan silika titania dikarakterisasi dengan analisis spektrofotometri infra merah dan difraksi sinar-X. Hasil analisis spektrofotometri infra merah menunjukkan bahwa telah terjadi interaksi antara silika dan titania dengan kitosan. Kristalinitas komposit kitosan silika titania lebih rendah dibandingkan dengan kitosan silika maupun kitosan sendiri. Hal ini menandakan bahwa interaksi antara partikel silika dan titania meningkatkan ketidakteraturan struktur kitosan.ABSTRACTChitosan-silica and chitosan-silica titania composites have been prepared by mixing chitosan solutions with silca sol and silica titania sol for antibacterial application. Chitosan were prepared by deproteination, demineralisation and deasetilation of chitin from Crab shells. The silica sol and silica titania sol were prepared by sol-gel method. The composites were characterized using Infra Red spectrophotometry and X-ray Diffraction methods. The  Infra Red study of the composites showed that there is interaction between silica and titania with chitosan. The crystallinity of chitosan silica titania and chitosan-silica was decreased, compared with chitosan, that is showed the increasing of stucture dissorder of composites material.
Adsorpsi lon Logam Zn(II) pada Bead Kitosan dari Cangkang Udang Windu (Penaus Monodon) (ADSORPTION OF ZrNK(II) METAL ION ON CHITOSAN BEAD FROM SHELL SHRIMP (Penaus monodon)) Sari Edi Cahyaningrum; Narsito Narsito; Sri Juari Santoso; Rudiana Agustini
Jurnal Manusia dan Lingkungan Vol 15, No 2 (2008): Juli
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18682

Abstract

ABSTRAK Telah dibuat dua macam adsorben kitosan dan bead kitosan dari cangkang udang windu (Penaus monodon) sebagai bahan dasar untuk adsorpsi ion logam Zn(Il). Adsorben kitosan dibuat melalui deproteinasi, demineralisasi diikuti deasetilasi, sedangkan bead kitosan merupakan hasil penggembungan terhadap kitosan. Beberapa parameter adsorpsi seperti pH, laju adsorpsi, dan kapasitas adsorpsi ion logam Zn(ll) dipelajari. Penelitian ini diawali dengan identifikasi secara spektroskopi infra merah terhadap gugus fungsional adsorben yang diperkirakan berfungsi sebagai situs aktif adsorpsi. Model kinetika adsorpsi orde satu yang mendekati kesetimbangan digunakan sebagai dasar untuk mengestimasi laju adsorpsi, sedangkan model isoterm adsorpsi Langmuir digunakan untuk menetapkan kapasitas adsorpsi. Hasil penelitian menunjukkan bahwa adsorpsi ion logam Zn(ll) pada kitosan serbuk dan bead kitosan mempunyai pH optimum yang sama. Laju adsorpsi ion logam Zn(II) pada bead kitosan secara signifikan lebih cepat dibanding pada kitosan. Proses swelling meningkatkan kapasitas adsorpsi ion logam Zn(ll) pada kitosan. ABSTRACT Two typies of adsorbent , i.e: chitosan and chitosan bead using shell of shrimp (Penaus monodon ) as raw materials, for Zn(II) metal ion adsorption. The chitosan was made by deproteination, demineralization followed deacetilation, whereas the chitosan bead was prepared by swelling the chitosan. Adsorption parameters such as, the adsorption rate, and the capacity of adsorption were determined. This work was started with the Infra Red Spectroscopy identification of functional groups with are expected to be the adsorption activ sides. The adsorption kinetic orde one at eqilibrium model  was applied in the estimation the adsorption rate, whereas the isoterm adsorption Langmuir model were employed in the determination of the capacity in adsorption. The results show that adsorption of metal ion Zn(II) on chitosan and chitosan bead have the same of optimum pH. The adsorption rate of metal ion Zn(II) on chitosan bead significant faster than on chitosan. Modification with swelling process enhanced the adsorption capacity Zn(II) on chitosan.
Characterization and antibacterial activity test of chitosan-silver nanoparticle gel and binahong leaf extract (Anredera cordifolia (Tenore) Steen) against Staphylococcus aureus Indira Dwi Aulia; Sari Edi Cahyaningrum
Jurnal Pijar Mipa Vol. 18 No. 4 (2023): July 2023
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v18i4.4994

Abstract

Binahong leaf is one of the medical plants that contain alkaloids, saponins, and flavonoids, which directly act as antibiotics. Additionally, chitosan and silver nanoparticles also possess antibacterial properties. This study aims to determine the antibacterial activity of a gel preparation against Staphylococcus aureus bacteria. It was a true experimental study with data collection carried out at the inorganic level. This study's results show that the gel preparation's organoleptic test meets the requirements for skin pH of 4.5-6.5. The spreading test meets the need for good distribution on the skin, which is 5-7cm. The antibacterial activity test showed that the largest diameter of the inhibition zone was observed at concentration F5 (5% binahong leaf extract), which was 13.4 mm.
Synthesis and characterization of hydroxyapatite-nanosilver-clove oil (Eugenia Caryophyllus) as antibacterial in toothpaste preparations against streptococcus mutans bacteria Browi Nugroho; Sari Edi Cahyaningrum
Jurnal Pijar Mipa Vol. 18 No. 4 (2023): July 2023
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v18i4.5325

Abstract

The formation of dental caries results from the activity of Streptococcus mutans bacteria. One of the efforts to prevent dental caries is using toothpaste containing antibacterial compounds such as hydroxyapatite-nanosilver-clove oil. This study aims to synthesize and characterize hydroxyapatite-nanosilver-cloves oil as an antibacterial that causes dental caries. In this study, physical properties were tested, namely, particle size with PSA, spreadability, stickiness, homogeneity, and chemical properties tested in functional groups with FTIR, pH, and antibacterial activity with the disc diffusion method. This study used 2% hydroxyapatite, ten ppm nanosilver, tween 80, xanthan gum, 70% sorbitol, sodium benzoate, sodium lauryl sulfate, sodium saccharin, calcium carbonate, peppermint, distilled water, clove oil with variations in the addition of concentrations of 1%, 2%, 3%, and 4%. The synthesis results produced a toothpaste preparation made from hydroxyapatite-nanosilver-clove oil, as evidenced by the results of FTIR characterization showing the presence of OH-, CO32-, PO43-, C-H, C-O, and C=O functional groups. PSA results show hydroxyapatite-nanosilver-clove oil has an average size of 4464 nm which is not included in the nanoparticle category (<100 nm). The toothpaste spreadability test resulted in a spread diameter of 5-6 cm, which met the requirements for paste spreadability (5-7 cm), while the adhesion test resulted in adhesion (7-12 seconds) which has not met the ideal requirements (1-6 seconds). The pH measurement resulted in a toothpaste pH of 8.8-9.0 per SNI (4.5-10.5). The homogeneity test of the toothpaste has met the requirements according to SNI, namely the absence of air bubbles, clumps, and separated particles. The antibacterial activity test showed that the hydroxyapatite-nanosilver-clove oil toothpaste formulation was able to inhibit the growth of S.mutans bacteria with the highest inhibition diameter in the variation of adding 4% concentration of clove oil, namely 14.99 mm with normally distributed data (p>0.05). One Way ANOVA statistical analysis showed that the treatment variation had a significantly different effect (p<0.05). These results indicate that adding clove oil into the formulation of hydroxyapatite-nanosilver-clove oil toothpaste can affect its chemical, physical, and antibacterial activity.
Detection of Knockdown-Resistance Homozygous Mutant C1534C using Allele-Specific Polymerase Chain Reaction in Aedes Albopictus and Aedes Aegypti Syananda Zahra Fadila; Ahmad Rudi Setiawan; Shifa Fauziyah; Safira Madaniyah; Eryantika Cipta Dewi; Sin War Naw; Sari Edi Cahyaningrum; Tukiran; Teguh Hari Sucipto
Folia Medica Indonesiana Vol. 59 No. 2 (2023): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i2.39802

Abstract

Highlights: This research analyzed a previously understudied subject in Surabaya, Indonesia, and discovered knockdown resistance (kdr) mutations in Aedes albopictus. The findings of this study can prompt further research, including bioassay testing and the development of more potent insecticides. Abstarct : Dengue hemorrhagic fever (DHF) has been endemic in Indonesia for decades. Insecticides are necessary to manage the transmission of the dengue virus. However, prolonged use of insecticides can lead to insecticide resistance. This study aimed to examine the genotype of mosquitoes using the allele-specific polymerase chain reaction (ASPCR) method. The ASPCR method was chosen for genotype detection due to its high sensitivity, affordability, and ease of design. Five mosquitoes were collected from human habitation in four different areas of Surabaya, Indonesia, namely Kranggan, Ulul Azmi Mosque, Ploso, and Kalijudan. Among them, three samples were identified as Aedes albopictus (A1, A2, and A5) and two samples were identified as Aedes aegypti (A3 and A4). The frequency of resistant alleles was analyzed using the Hardy-Weinberg package in RStudio version 2023.03.1. This study revealed that two mosquitoes carried homozygous mutant alleles with a band of 113 bp and three mosquitoes carried homozygous wild-type alleles with a band of 93 bp. Cysteine-to-cysteine (C/C) mutations and phenylalanine-to-phenylalanine (F/F) mutations at codon 1534 were observed in Aedes aegypti and Aedes albopictus mosquitoes. The homozygous mutant alleles were found in Kranggan, Surabaya, Indonesia. Further research is required to assess insecticide resistance and knockdown resistance (kdr)-like mutation by collecting more representative samples from larger areas within the region of Surabaya. Nevertheless, this study can be used as a reference for vector control and early prevention of dengue fever.
Understanding of Healthy and Halal Additive Criteria for MSME Actors in Gresik Regency: Pemahaman Kriteria Bahan Tambahan yang Sehat dan Halal Pada Pelaku UMKM di Kabupaten Gresik Nuniek Herdyastuti; Tukiran; Titik Taufikurohmah; Sari Edi Cahyaningrum; Ratih Dewi Saputri
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 4 (2023): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v7i4.15329

Abstract

Gresik Regency is very rich in its typical food involving UMKM. They were still use many synthetic additives such as dyes, flavors, and flavorings with the aim of beautifying the appearance and strengthening the taste. The continuous use of synthetic additives can interfere with health and also many have not met halal criteria. They do not know or apathetic to the dangers posed by the use of additives, so it is necessary to carry out socialization related to healthy and halal ingredients. The methods used are counseling and practice of identifying ingredients based on nutritional composition and critical ingredients and conducting evaluations through response questionnaires. The results showed that 36.4% of participants did not know how to identify halal products; 36.5 – 45.5% still use synthetic additives and 90.9% their knew the effects of using synthetic additives. The practice showed that UMKM do not understand critical materials so that follow-up needs on understanding critical materials.
Co-Authors 'Aisy, Khulud Rihadatul , INSIYAH Achmad Fitriadi Akbar Afifah, Fifi Ahmad Rudi Setiawan Akbar, Achmad Fitriadi Amaria Amaria Amaria Amaria Amaria Amaria Amaria Amaria Angelo Ranamanggala, Jonathan Browi Nugroho Dewinta Intan Laily Dharmawan, Anggi Putra Dina Kartika Maharani Dina Kartika Maharani Dina Kartika Maharani ERIN WARDIANA, APRITA Eryantika Cipta Dewi Fifi Afifah Filza Qurrota 'Aini Fitriari Izzatunnisa Muhaimin Fitriari Izzatunnisa Muhaimin Hanifa Rachmah Kamila Heryastuti, Nuniek I GUST MADE SANJAYA ILHAM FADILA RAMADHAN, MUHAMAD Indira Dwi Aulia Jonathan Angelo Ranamanggala Lawarti, Riska Amelia Lazuardi, Gilang Pandu Leny Yuanita Leny Yuanita Lestari, Nanda Revita Dwi Lindawati, Zeti Luklukul Fitriyah Marena, Betari Maria Monica Sianita Maria Monica Sianita Maya Dany Puspitasari Mudasir Muhaimin, Fitriari Izzatunnisa MUSLIMIN IBRAHIM Mustaji Mustaji Mustaji Mustaji, Mustaji N Narsito Nanda Onky Stefani Narsito Narsito Narsito Narsito Narsito Narsito Narsito Narsito Novita Listiyani Nuniek Herdyastuti Nuniek Herdyastuti Nuniek Herdyastuti Nuniek Herdyastuti Nuniek Heryastuti Nur Qomariah Nurhayati Nurhayati Nurtikasari, Ayu Wika NURUL HIDAJATI Nurul Hidajati Perintis Gita Susanti Prima Retno Wikandari Ratih Dewi Saputri Riska Amelia Lawarti Rudiana Agustini Rudiana Agustini Rusmini Rusmini Rusmini Rusmini Rusmini Rusmini Sabirin Matsjeh Safira Madaniyah Satriawan, Muhammad Shifa Fauziyah Sin War Naw Soraya, Fathimah Sri Juari Santoso Sri Juari Santoso Sri Juari Santoso Sri Juari Santoso Sri Juari Santoso Sukarmin Suwandi, Sukma Yanti Putri Ayu Syananda Zahra Fadila Teguh Hari Sucipto, Teguh Hari Titik Taufikurohmah Titik Taufikurohmah Titik Taufikurrohmah Titik Taufikurrohmah Tri Joko Raharjo Tsani Astuti Tukiran Tukiran Wahyudi, Ainaia Deaqiila Wijayanti, Alvia Rachma Yasfin Shafa Nurulisia Yossy Nur Annisa