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Journal : BERITA BIOLOGI

METIL EUGENOL, KHEMOTIPE DARIMINYAK ATSIRI Melaleuca spp. (MYRTACEAE) YANG TUMBUH DIKEBUN RAYA CIBODAS Murningsih, Tri; Chairul, Chairul; Kuncari, Emma Sri
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.403 KB) | DOI: 10.14203/beritabiologi.v9i6.859

Abstract

The essential oils of the fresh leaves of Melaleuca spp. (M. alternifolia Cheel, M. ericifolia Sm., M. squarrosa Donn, and M.genistifolia Sm.) from Cibodas Botanical Garden, Indonesia were isolated by a hydrodistillation method and analysed by a gas chromatography/mass spectrometry (GC/MS) technique. The various Melaleuca species showed differences within their relative essential oil compositions. The oils from all samples were of the methyl eugenol (10.30-71.25%) chemotype. The others major constituent were terpinen-4-ol (38.49%), linalool (19.65%) and bis(2-ethylhexyl)phtalate (66.09%) in the M. alternifolia, M. ericifolia, and M. genistifolia respectively.
ISOLASI GLUKOMANAN DARI DUA JENIS ARACEAE: TALAS {Colocasia esculenta (L.) Schott} DAN Iles-iles (Amorphophallus campanulatus Blumei) Chairul, Chairul; Chairul, Sofnie M
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.637 KB) | DOI: 10.14203/beritabiologi.v8i3.793

Abstract

Talas {Colocasia esculenta (L.) Schott} and Iles-iles (Amorphophalus campanulatus Blumei) have been used as source of carbohydrate especially in tropical regions, mainly in East Indonesia. Talas and iles-iles, that contains carbohydrate and several substances for instance glucomannan would form viscous liquid and were used as emulgator, capsule cosmetics and dietary.The research was conducted to separate glucomannan from starch of talas and iles-iles fresh and dry (powder).The methods base on speed of revolution (centrifuge) in 5000 to 19000 rpm and the duration of rotation was 10 to 40 minutes and the filtrate was precipitated by using several alcohols (methanol, ethanol, propanol, isopropanol and isobutanol). The results showed that speed of rotation 17 x 1000 rpm with 10 minutes rotation resulted in good glucomannan separation from starch.Ethanol and isopropanol were the best solvent to separate glucomanan from dissolved starch in the filtrate with ratio 2 : 3 and for dry material, and the maximal agitation reached after 2 hours. Glucomannan content on fresh material was higher than as in talas (4.08 %) and iles-iles (5.64 %), compared to these in powder (dried) of talas (3.87 %) and iles-iles (5.41 %). The purerity of isolated glucomannan was higher compared to control.
PERBANDINGAN KOMPONEN KIMIA PENYUSUN MINYAK ATSIRI SIRIH LIAR {Piper ornatum) YANG BERASAL DARI SULAWESI SELATAN DAN PULAU SERAM DENGAN SIRIH BIAS A (Piper betle) Sulianti, Sri Budi; Chairul, Chairul
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1223

Abstract

Comparison study on the chemical components of P. ornatum (Piperaceae) volatile oil, collected from Enrekang (South Celebes)and Sepa Village (Ceram Island, Moluccas) had been carried out by water distillation and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Those plants had exotic performance. The sample from South Celebes had original red color leaves and strong aromatic smell, but one from Ceram Island has green color leaves and weak aromatic smell. The results showed that volatile oil content of P. ornatum from Enrekang has higher than Sepa Village (0.94% and 0.81%), while the volatile oil of common piper (P.betle) was around 0.9-1.2%. GC-MS analysis determined that volatile oil of P. ornatum had 22 chemical components. Both volatile oil indicated similar peak fragmentation, in different intensities. The major component of P. betle and P. ornatum was caryophyllene i.e. 30.01% in the red leaf, 29.41% in the green leaf and 31.05% in P. betle. Some of minor components of P.ornatum were different, from P. betle.
Di Sela-Sela Laboratorium dan Plot Eksperimen MENGENAL HPLC: PERANANNYA DALAM ANALISA DAN PROSES ISOLASI BAHAN KIMIA ALAM Murningsih, Tri; Chairul, Chairul
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (751.616 KB) | DOI: 10.14203/beritabiologi.v5i2.1161

Abstract

The development of method to explore natural chemical product initially from plants were started from the beginning and generously from time to time from the conventional ones until sophisticated equipments.The value of plants as food,cosmetic and medicines resources needs specific instruments and methods for determining the quantity as well as quality of the materials in plants.This paper describe in detail one of the sophisticated equipments commonly used for analyzing and isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC).
EFEK HYPOGLIKEMIK EKSTRAK ALKOHOL HERBA MENIRAN (Phyllanthus niruri L.) PADA KELINCI PUTIH JANTAN Chairul, Chairul; Jamal, Yuliasri; Zainul, Zuherti
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.884 KB) | DOI: 10.14203/beritabiologi.v5i1.1103

Abstract

A study on the influences of alcohol extract of Meniran or Phyllanthus niruri L. (Euphorbiaceae) to blood glucose concentration in rabbits (Oxytolagus cuniculus) had been carried out using glucose tolerance method.The extract was administered orally at 10, 20, 30, 40 and 50 mg/kg BW.Prior to tfie extract administration, all rabbits were put on hyperglycemic condition by injecting glucose solution intravenally at dose of 0.5 g/kg BW.The blood glucose concentration was measured every one hour starting at 2 hours before and until 7 hours after administration. Antidiabetic drug,tolbutamid 250 mg/kgBW.was used as control.The results indicated that extract with dose at 30 mg/kg BW gave a hypoglycemic effect similar to control (Tolbutamid). The blood glucose decreased regularly as the extract dose being higher.Using statistical analyses showed significant differences among the groups, especially after the 2 and 3 hours of treatment (P<0.05).
PENGARUH PEMBERIAN EKSTRAK ALKOHOL AKAR ILALANG {Imperata cylindrica L.) TERHADAP PENURUNAN SUHU TUBUH TIKUS PUTIH JANTAN Chairul, Chairul
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.434 KB) | DOI: 10.14203/beritabiologi.v5i2.1158

Abstract

Antipyretic effect of alcohol extract ofilalang flmperata cylindrica L) root on white rat males had been studied using fever tolerance method.Experimental animals were induced to fever by injecting 0.9 ml/kg BW vaccine kotipa orally.Alcohol extract 5, 10, 30, 50,100 and 150 mg/kg BW was given one hour after injection.The body temperature was measured before injection and every half an hour until the fifth hour after injection.Paracetamol 200 mg/kg BW was used as control.Data were analyzed using ANOVA with the body temperature (T), the time interval (P) and extract doses being given (C) as parameters.The results showed that body temperature increased TC from the basal temperature at the third hour after injection of vaccine.The body temperature did not change significantly at the lower extract doses(5-30 mg/kg BW)(P>0.05),while higher doses (30-150mg/kg BW) linearly lowered the body temperature significantly (P<0.05).The extract dose at 50 mg/kg BW lowered the body temperature similar to paracetamol 200 mg/kg BW.
IDENTIFIKASI SECARA CEPAT BAHAN BIOAKTIF PADA TUMNUHAN DI LAPANGAN Chairul, Chairul
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7809.006 KB) | DOI: 10.14203/beritabiologi.v6i4.3461

Abstract

New drug discovery from natural products (bio-prospecting) is not an easy works and takes time, big budget, human resources etc. some important approaches must be taken in order to get success. A preliminary observation of biologycally active components is impotant approach in order that more selective in collecting research materials in the field. This approach is purposed to pacilitate (make easier) the next step research process later in the laboratory level. Several Methods of preliminary observaton of biologyvcally active components had been carried out in the field, but the general guideline of fast observation had been used e.g. 1). Etno-botany (Etnomedicine and Etnopharmacology), 2). Chemotaxonomy, 3). Organoleptic, 4). Chemical (reagent kit) and 5).Simple bioassay in the field (fish posion test, insecticide). Besides that collecting the herbarium specimen as well as research materials for any scienfitic and laboratory works. This paper described these approaches in order to make useful methods to researchers, who go to the field and they could collect more selective research materials before bring them to laboratory.
ANALISIS KOMPONEN KIMIA DAUN WATI {PIPER METHYSTICUM FORST. F) Agusta, Andria; Jamal, Yuliasri; Chairul, Chairul
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1276

Abstract

Wati(Piper methysticum Forst.f.)leaves has been used as narcotic in some parts of Irian Jaya since longtime ago. The GCMS analysis of wati leaves organic extracts(hexane,chloroform, methanol)has detected 48 components consisted alkanes,oxygenated hydrocarbons, fatty acid, steroids and alkaloids,including 5 major components, i.e. dihydro kawain,anisol p-undecyl, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-on, levulinic acid and one of unknown component.Forty-three other minor components will be discussed.
Co-Authors , Deviona, , Ade Ayu Oksari Agrina, Agrina Amanda, Falencia Mauri ANDRIA AGUSTA Annisa, Syarifah Sari Apriliani, Prapita Ardesta, Ribbi Sukma Armaini, Armaini Arofi, Habib Asral, Asral Astrandana, Yudi Besri Nasrul Br. Silaban, Nesia Rachel Lina Dahelmi Dahelmi Dahlan Tampubolon Darliansyah, Danny Desi Heltina Dewi Kusrini DEWI WULANSARI Dirgantara, Doni Ari Drastinawati Drastinawati, Drastinawati Emelda, Retta Emma Sri Kuncari Erizal Mukhtar EVELYN EVELYN Fadhlan, Adli Fauziah Syamsi Febiani, Hartika Febrialismanto, Febrialismanto Febriamansyah, Thoriq Alfath Febriansyah, Beni Fermi, Iwan Filda, Aiska Fitria Fitria Geovana, Savira Geovani Meiwanda Ginting, Theresia Ellyandra Destri Gunawan Tabrani Hasibuan, Muhammad Ilham Febrian Mahodum Hendra, Alfino Hengki Firmanda Hijran, Muhammad Hafiyyan Naufal Indra Lesmana Indriana, Hanifah Irvan Fadli Wanda Izmiarti Izmiarti, Izmiarti Karimi, Kasman Khairunnisa, Syakira Nada Khoirul Amru Nst, Khoirul Amru Komalasari Komalasari Kurniawan, Abdy Kusbiantoro, Sigit Lia Nazliana Nasution M. Idris Mairawita, Mairawita Maria Peratenta S, Maria Peratenta Maria Peratenta Sembiring Marpaung, Masdalena Marzuki, Salman Miftahul Jannah Mildawati Mildawati Muchtar, Erizal Muh Wawan Junuansyah, Muh Wawan Muhammad Furqan, Muhammad Najah, Muthiya Safinatun Nelvia Nelvia Ningsih, Lisa Septia Nurainas Nurainas Nurwijayanti Nuryanti, Lidya Ohi, Hiroshi Pertiwi, Vera Presi, Familindo Putri, Alivia Hardana Putri, Novia Anas Radith Mahatma Rahimmi, Anna Fithra Rahmah, Yulia Rianti, Rianti Ristia Ningsi, Maya Rohaya, Rohaya Romi Kurniadi Saefudin Saefudin Safitri, Nadira Sakutteri, Riza Salsabilla, Dhiyya Sandy, Athifah Faradilla Satria, Fitra Sayyidhani, Muhammad Hisna Sefmaliza, Rizka Serly Marselina Arifin, Serly Marselina Silaban, Kardiman Silvia Reni Yenti Simanjuntak, Billy Oktavianus Simanjuntak, Tagonna Serasi Asnita Sinaga, Raihan Daffansyah Sitinjak, Jufri Suanto Sitompul, Diantika Elena Sofnie M Chairul Sofnie Marusin Soleh Kosela Solfiyeni Solfiyeni Sri Budi Sulianti, Sri Budi Sri Endang Kornita Sri Rezeki Muria Suwirmen, Suwirmen SYAIFUL BAHRI Syamsuardi Syamsuardi Syelvia Putri Utami Tasman, Annaya Qamara Tesri Maideliza Tibrani Tibrani, Tibrani Tri Murningsih, Tri Triani, Leni Villarul, Trya Nungky Wahyu Hidayat Wahyu, Pri Widodo, Prio Wilson Novarino Wulandari, Siti Rahmi Indri Wulandari, Zarah Ayu Yasminto, Habib Maulana Yasra, Firham Yaumil Mutia Akhir, Yaumil Mutia Yayan Setiawan, Yayan Yelmida Azis Yuanita, Mira Yuliana Yuliana Yulianda, Aldi Yuliasri Jamal Yusnimar Sahan Zahtamal Zahtamal Zainul, Zuherti Zulaspita, Witri Zulfansyah Zulfansyah Zultiniar, Zultiniar