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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) VALENSI Jurnal Bios Logos CHEMISTRY PROGRESS JURNAL KIMIA SAINS DAN APLIKASI JURNAL TEKNOLOGI LINGKUNGAN IPTEK Journal of Proceedings Series Jurnal Riset Kimia SEMIRATA 2015 Jurnal Kimia Mulawarman IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Science and Technology Indonesia Jurnal Penelitian Sains Chimica et Natura Acta Indonesian Journal of Pure and Applied Chemistry Sains Natural: Journal of Biology and Chemistry JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) Jurnal Tanah dan Iklim
Charlena
Departemen Kimia,Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor Kampus IPB Dramaga Jl.Agatis Wing 2 Level 4, Bogor 16144, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

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Journal : hayati journal of biosciences

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Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Yani, Mohamad; Charlena, Charlena; Mas?ud, Zainal Alim; Anas, Iswandi; Setiadi, Yadi; Syakti, Agung Dhamar
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Mohamad Yani; Charlena Charlena; Zainal Alim Mas’ud; Iswandi Anas; Yadi Setiadi; Agung Dhamar Syakti
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Co-Authors Aditya, Muhammad Hafizh Agung Dhamar Syakti Ahmad Syahreza Akhiruddin Akhiruddin Maddu Aldes Lebani Ananda Novita Hi Pattu Armi Wulanawati Aslihati Aslihati Chonny Damayanti Daniel Putra Oktavianus Laia Dede Heri Yuli Yanto Dicky Iswara, Muhammad Dila Ardiansyah Dyah Iswantini Fadila, Rina Fitriana Fendy, Fendy Hanies Ambarsari Henny Purwaningsih Ida Ayu Putu Sri Widnyani Irma Herawati Suparto Irma Herawati Suparto Irma Herawati Suparto Iswandi Anas Iswandi Anas Iswara, Muhammad Dicky J. SRI ADININGSIH Joni Prasetyo, Joni Khefanny, Yemima Chellyne Khofiyatuzziyadah, Alif Aryan Komar Sutriah L.I NURHAYATI LADY R. WIDOWATI Manasye Erlangga Marita Soebrata, Betty Mas?ud, Zainal Alim Maulida Oktaviani Moch. Irgham Zuhfria Moh. Yani Nazriati Nazriati dkk. Niken Dyah Wanodyanti Nurmawan, Cucu Putri Nurul Afiati Aflakhah Purwaningsih, Henny Purwantiningsih Ravena Ravena Rohma, Fitria Ainun Rosdiana, Tina S. SRI DWININGSIH Sari, Nurwita Mukti Setyanto Tri Wahyudi Sianipar, Rut Novalia Rahmawati Sulistioso Giat Sukaryo Susi Sulistia, Susi Tatang Hidayat Tetty Kemala Tina Rosdiana Wisaeni Intannia Wulan Islamia Yadi Setiadi Yadi Setiadi Yessie Widya Sari Zahra, Imelda Zainal Alim Mas'ud Zainal Alim Mas’ud