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Journal : Jurnal Kimia (Journal of Chemistry)

HIDROLISIS RUMPUT LAUT (Glacilaria sp.) MENGGUNAKAN KATALIS ENZIM DAN ASAM UNTUK PEMBUATAN BIOETANOL Yohanes Armawan Sandi; Wiwik Susanah Rita; Yenni Ciawi
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.749 KB) | DOI: 10.24843/JCHEM.2016.v10.i01.p02

Abstract

The aim of this research is to determine the effect of enzyme and acids concentration on the yield of glucose produced in the hydrolysis of Glacilaria sp. in the production of bioethanol. The concentrations of cellulase used were 200 units/mL, 400 units/mL, 600 units/mL, 800 units/mL and the concentration of sulphuric acid (H2SO4) and hydrochloric acid (HCl) used were 1%, 3%, 5%, 7% (w/v). The concentration of reduction sugar was determined using Anthrone and analyzed using UV-Vis spectrophotometry and the determination of ethanol concentration was carried out by using gas chromatography. The results showed that the contents of reducing sugar produced by sulphuric acid (H2SO4) hydrolysis were 26,19%; 36,69%; 41,40%; 45,0% (v/v), by hydrochloric acid (HCl) were 12,12%; 14,03%; 15,17%; 16,50% (v/v), and by cellulase enzyme were 46,15%; 46,73%; 47,68%; 48,25% (v/v). Optimum concentration of reducing sugar produced by hydrolysis using 800 units/mL cellulase was 48,25% (v/v). The optimum length of fermentation to produce bioethanol using Glacilaria sp. as raw material was 5 days. In the fermentation, inoculum with a concentrations of 5% and 10% (w/v) produced 0,85% and 1,51% (v/v) ethanol.
PENGARUH KONSENTRASI AMMONIUM SULFAT ((NH4)2SO4) SEBAGAI SUMBER NITROGEN TERHADAP PRODUKSI BIOETANOL BERBAHAN BAKU Glacilaria sp. Ni Putu Widayanti; Wiwik Susanah Rita; Yenni Ciawi
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 1 Januari 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.963 KB) | DOI: 10.24843/JCHEM.2013.v07.i01.p01

Abstract

The aims of this research are to determine the concentration of reducing sugar and starch in Glacilaria sp. and to investigate the influence of the concentration of ammonium sulfate as nitrogen source on the production of bioethanol using Glacilaria sp. as the carbon source. The concentrations of ammonium sulfate employed were 0,25%; 0,50%; 0,75%; and 1% (w/v). The methods employed in this research were the determination of water content, glucose and starch concentration using Nelson-Somogyi method, determinating the optimum duration of fermentation, pH measurement, and the determination of  ethanol concentration. The results showed that the concentrations of reducing sugar and starch in Glacilaria sp. are 17,14% and 15,42%. The concentration of (NH4)2SO4, which produced the optimum concentration of ethanol (1,04% (v/v) on seventh day), was 1% (w/v). The fermentation media containing (NH4)2SO4 of 1%; 0,75%; 0,5%; and 0,25% (w/v) produced optimum concentrations of ethanol, which were 0,96% (v/v) on fifth day; 0,78% (v/v) on seventh day; 0,74% (v/v) on seventh days; and 0,73% (v/v) on seventh day, respectively. The optimum concentration of (NH4)2SO4 was 1% (w/v), which produced maximum concentration of  bioethanol from Glacilaria sp., of 0,96% (v/v).
IDENTIFIKASI SENYAWA ANTIBAKTERI PADA DAUN KECAPI (Sandoricum koetjape (Burm.f.) I M. Dira Swantara; Yenni Ciawi
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 2 Juli 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1166.082 KB)

Abstract

Identification of antibacterial compound for Micrococcus luteus dan Eschericia coli bacteri have beenconducted to kecapi leaf extract (Sandoricum koetjape (Burm.f.)). Extraction of that compounds was carried out bymaseration methods using 70% etanol. Separation of the fraction by liquid-liquid partition (n-hexane, ethyl acetateand chloroform) and two step column chromatographic method using silicagel as stationary phase and ethyl acetate –chloroform (3:7;2,5:7,5; dan 2:8) as first eluent and ethyl acetate – chloroform (2:8; 1,5:8,5; dan 1:9) as secondeluent. The active fraction (2.1 and 2.3 fraction) were identified by gas chromatography-mass spectroscopy (GCMS).The 2.1 fraction contain 3 compound which are heksil n-valerate (C11H22O2); 2,3-dihidrobenzofurane (C8H8O);and 2,6-dimetoxy fenol (C8H10O3). The 2.3 fraction contain 2 compound which are ester dioctilehexadioat(C22H42O4) with M+ = 370 and basic peak equal to 129 and 3,5-di-tert-buthyl-4-hydroxy-toluene (C15H24O).