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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Farmasi Indonesia Current Biomedicine Policy Brief Pertanian, Kelautan, dan Biosains Tropika Biodiversitas Journal of Biological Diversity
Okti Nadia Poetri
Division Of Microbiology Medic, Department Of Animal Disease And Veterinary Public Health, Faculty Of Veterinary Medicine, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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THE POTENTIAL OF ADJUVANT AGAINST PRODUCTION OF ANTISTREPTOCOCCAL IMMUNOGLOBULIN Y (IGY) IN AQUACULTURE Rizkiantino, Rifky; Wibawan, I Wayan Teguh; Pasaribu, Fachriyan Hasmi; Soejoedono, Retno Damajanti; Poetri, Okti Nadia; Arnafia, Wyanda; Sasi, Kris Damar; Reisinta, Dinda
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.16911

Abstract

This study was conducted to explore the potential of adjuvant for the production of immunoglobulin Y (IgY) as antistreptococcosis in layer chicken with mass production orientation. Enterococcus faecalis which causes streptococcosis in the red tilapia was selected as a candidateantigen. The production of immunoglobulin Y (IgY) was carried out on Isa Brown layer chickens and aged around 20 weeks. Furthermore, thechickens were grouped into four groups (A, B, C, and D groups), each consisting of three chickens based on the type of adjuvant, while twochickens were used as a control group. Each group was treated by giving MONTANIDE ISA 71R VG adjuvant (A), Freund's adjuvant (B), aluminum potassium sulphate adjuvant (KAl(SO4)212H2O) concentration of 50 ppm in pH 7 (C), and only antigens without adjuvant (D). Chickens were kept for 35 days and each week was checked for presence the IgY antigen in the serum and egg yolk. Booster was conducted on 14th and 28th days of maintenance. The results showed that IgY in treatment group A was detected on day 28 in the serum and day 35 in the yolk. Whereas the treatment group B could be detected on day 35 in the serum. However, the IgY was not detected in the serum and yolk in C, D, and control groups until the end of the maintenance. Based on the results, it can be concluded that the appearance of IgY in serum and yolk in a relatively fast time is obtained in the combination of Enterococcus faecalis antigen with the emulsion of water-in-oil adjuvant (SEPPICMONTANIDE ISA 71R VG) compared to the other types of adjuvant that use in this study.
Evaluation of influenza-specific immunoglobulin Y stability in liquid, solid, and cream-based body care formulations Poetri, Okti Nadia; Setyawati, Yusefin Marcelina; Kal Alfin, Zabrino Wahid; Kalsum, Salsabila; Fathurrahman, Fathurrahman; Rong, Chin Xiao; Nugraheni, Indi Amalia; Kuswanti, Andrea Septiandra
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.98

Abstract

Background Specific immunoglobulin Y (IgY) is widely used in immunotherapy, with expanding applications in body care products. Topically applied influenza-specific antibodies may provide an alternative strategy for preventing respiratory infections. Objective This study aimed to evaluate the stability and biological activity of influenza-specific IgY in various body care products. Methods The biological activity of influenza-specific IgY was evaluated in various body care products at different concentrations. Commercial face mists contained 5% and 10% w/v IgY, face mists and nasal sprays contained 0.5% w/v IgY, lip balm, sunscreen, and hand cream contained 0.1% and 0.25% w/v IgY. The products were stored at room temperature for four weeks, and organoleptic changes were monitored weekly. IgY activity was assessed by enzyme-linked-immunosorbent-assay (ELISA). Results In both face mist products, the aroma gradually became fishy, the color remained stable, and turbidity increased. The biological activity of IgY was still detectable by ELISA at concentrations of 0.5%, 5%, and 10%. In nasal spray, no changes in aroma, color, or turbidity were observed, but IgY activity was not detectable. In lip balm, sunscreen, and hand cream, only slight color changes occurred without any change in aroma, and IgY activity was not detected. Conclusion Influenza-specific IgY retained biological activity in face mist formulations at higher concentrations (≥0.5%) despite observable changes in aroma and turbidity. In contrast, IgY activity was not detected in nasal sprays, lip balm, sunscreen, or hand cream, suggesting that the formulation type and IgY concentration significantly influence its stability and detectability in body-care products.
Preparation of avian influenza H5N1 and Newcastle disease antigens for hemagglutination inhibition assay applications Okti Nadia Poetri; Syaharani Salsabila; Nada Adinda Pebriana
Current Biomedicine Vol. 4 No. 1 (2026): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.4.1.8

Abstract

Background Avian influenza (AI) and Newcastle disease (ND) are major poultry diseases in Indonesia, where monitoring of vaccination efficacy commonly relies on the hemagglutination inhibition (HI) assay. The HI assay requires viral antigens, which are generally obtained commercially from PUSVETMA. However, independent antigen preparation would be beneficial for private laboratories. Objective This study aimed to prepare and validate in-house AI and ND antigens as HI assay reagents. Methods Viruses were propagated in embryonated chicken eggs (ECE), inactivated using buffered neutral formalin (BNF), precipitated with polyethylene glycol (PEG-6000), and preserved with glycerol. Validation was conducted by parallel HI testing of 24 chicken sera for AI and 22 chicken sera for ND using both in-house and PUSVETMA’s antigens. Antibody titers were analyzed using analysis of variance (ANOVA), with sensitivity (Se), specificity (Sp), and kappa (κ) tests performed for agreement.   Results ANOVA revealed no significant differences in geometric mean titers between in-house and PUSVETMA’s antigens (P<0.05). Both the AI and ND in-house antigens demonstrated Se and Sp values of 100% and κ values of 1, indicating perfect agreement. Conclusion These findings confirm that in-house AI and ND antigens are comparable to their commercial counterparts and can serve as reliable and cost-effective reagents for HI testing in private laboratories.
Intervensi Dampak Penyakit Kaki Dan Mulut Berbasis Biaya Manfaat Chaerul Basri; Etih Sudarnika; Okti Nadia Poetri; Atsmarina Widyadhari; Cut Desna Apriana; R. Dikky Indrawan; Asep Rakhmat; M. Gumilang Pramuwidyatama
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 5 No. 3 (2023): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0503.719-722

Abstract

Upaya pengendalian dan pemberantasan PMK di Indonesia jika dilaksanakan dengan program vaksinasi dengan kecepatan seperti yang berlangsung saat ini maka akan menyebabkan waktu pemberantasan yang lebih lama dan kerugian ekonomi yang besar. Perbaikan program vaksinasi dengan kecepatan yang ideal selain dapat memperpendek waktu pemberantasan juga akan memberikan dampak keuntungan ekonomi yang sangat besar. Berdasarkan hasil studi ini direkomendasikan agar upaya pengendalian dan pemberantasan PMK di Indonesia sebaiknya program yang dilaksanakan mengacu kepada skenario vaksinasi dengan kecepatan yang ideal. Dalam penyusunan dan pelaksaan program pengendalian di Indonesia dapat menggunakan program pengendalian yang dikembangkan di Filipina sebagai bahan pertimbangan karena sudah terbukti secara efektif berhasil memberantas PMK dari negara kepulauan seperti di Indonesia
COMPARATIVE ANALYSIS OF PROBING AND CONTRAST RADIOGRAPHY FOR GENDER DETERMINATION IN RETICULATED PYTHONS (Malayopython reticulatus) Zhi Yien Tan; Okti Nadia Poetri; Mokhamad Fakhrul Ulum
BIOTROPIA Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.3.2278

Abstract

Article Highlights- Accurate gender identification supports effective breeding programs and ensures snakes' welfare.- This study emphasizes that probing is more reliable and efficient than contrast radiography for gender determination in reticulated pythons.- The probing method proved to have 100% accuracy in determining the gender of reticulated pythons, outperforming contrast radiography, which only achieved 75% accuracy. AbstractReticulated pythons, like most snakes, do not exhibit noticeable sexual differences, which makes gender determination necessary. Probing and contrast radiography were chosen as methods to determine sex and to compare their accuracy. Six reticulated pythons with body lengths of 128-167 cm, tail lengths of 5.4-9.6 cm, and body diameters of 1.4-3.4 cm were examined twice by probing and contrast radiography. The results showed that probing had 100% accuracy, with probe lengths ranging from 1.37-3.60 cm for male snakes and 0.3-0.76 cm for female snakes. Contrast radiography had 75% accuracy, with the hemipenis appearing as a white silhouette and female snakes appearing colorless. The accuracy rates highlight the effectiveness of probing over contrast radiography for reliable gender determination in reticulated pythons
Detection of infectious bronchitis-like virus isolated from psittacines at breeding facilities Ganita Kurniasih Suryaman; Retno Damajanti Soejoedono; Okti Nadia Poetri; Agus Setiyono; EKOWATI HANDHARYANI
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221017

Abstract

Abstract. Suryaman GK, Soejoedono RD, Poetri ON, Setiyono A, Handharyani E. 2021. Detection of infectious bronchitis-like virus isolated from psittacines at breeding facilities. Biodiversitas 22: 4261-4265. Captive breeding of parrots has been carried out in Indonesia to fulfill market demand for pets and support conservation efforts. However, there is limited information about Infectious Bronchitis Virus (IBV) in captive parrots. Therefore, this study examines IBV virus in psittacines and observes the pathological changes in inoculated chicken embryos. Cloacal swabs were obtained from 31 psittacine birds consisting of 15 sulfur-crested cockatoos (Cacatua sulphurea), 10 palm cockatoos (Probosciger aterrimus), two blue and yellow macaws (Ara ararauna), two green-winged macaws (Ara chloropterus), and two scarlet macaws (Ara macao). Swabs were also injected for 9 days to obtain specific pathogen-free (SPF) embryonated chicken eggs, and allantoic fluids were collected after 7 days. The residual cloacal swabs and allantoic fluid samples were tested for IBV by reverse transcription polymerase chain reaction (RT-PCR) targeting spike 1 glycoprotein (S1) and nucleoprotein (N) gene by primer set XCE2+/XCE2- and IBVN+/IBVN-, while the harvested inoculated embryos were examined for histopathological lesions by hematoxylin-eosin staining. Three and one allantoic fluid of C. sulphurea and P. aterrimus respectively were found positive for IBV by primer set IBVN+IBVN-. However, there was no significant difference in the inoculated embryo's gross anatomy, and the slight histopathological changes observed were not caused by the virus. Therefore, it is unclear whether the IB-like virus causes clinical disease manifestation in both Psittacine species or the chicken embryo. This is the first study carried out on IB-like viruses for both species.
Co-Authors Agus Setiyono Agustin Indrawati Aldo Yanuar Wuriyantara Anindita Sista Widyadhari Anindita Sista Widyadhari Annisa, Syifa Ari Rumekso Asep Rakhmat Atsmarina Widyadhari Cece Sumantri Chaerul Basri Cut Desna Apriana Dilas Pradana Dinda Reisinta Dwi Desmiyeni Putri Dwi Utari Rahmiati Eko Sugeng Pribadi Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fathurrahman Fathurrahman Ferry Ardiawan Ganita Kurniasih Suryaman Hadri Latif Hanandi, Sharon I Wayan T. Wibawan I Wayan Teguh Wibawan Kal Alfin, Zabrino Wahid Kalsum, Salsabila Koekoeh Santoso Kris Damar Sasi Kurnia Sari Setio Putri, Kurnia Sari Setio Kuswanti, Andrea Septiandra La Jumadin La Jumadin M. Gumilang Pramuwidyatama Melani Wahyu Adiningsih Mokhamad Fakhrul Ulum, Mokhamad Nada Adinda Pebriana Ni Luh Putu Ika Mayasari Novera Nirmalasanti Nugraheni, Indi Amalia Nur Khusni Hidayanto Nurjumaatun Nurjumaatun Putra, Raditya Pradana R. Dikky Indrawan Rahayu WoroWiranti Rahmasari, Ratika Rahmat Setya Adji Reisinta, Dinda Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damajanti Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Rifky Rizkiantino Rizkiantino, Rifky Rong, Chin Xiao Sasi, Kris Damar Setyawati, Yusefin Marcelina Sri Murtini Surachmi Setiyaningsih Sutriyo Suwarny Ruhi Syaharani Salsabila Trioso Purnawarman Wilbert Wylie Wyanda Arnafia Wyanda Arnafia, Wyanda Zhi Yien Tan