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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Berkala Penelitian Hayati Jurnal Kedokteran Hewan Jurnal Farmasi Indonesia Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Okti Nadia Poetri
IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Tanggap Antibodi terhadap Capsid Virus Penyakit Jembrana setelah Vaksinasi Lapang Sapi Bali di Kabupaten Sarolangun, Jambi Ferry Ardiawan; Okti Nadia Poetri; Nur Khusni Hidayanto; Ari Rumekso; Dilas Pradana; Surachmi Setiyaningsih
Acta VETERINARIA Indonesiana Vol. 11 No. 2 (2023): Juli 2023
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.11.2.167-174

Abstract

Penyakit Jembrana (JD) adalah penyakit prioritas nasional yang disebabkan oleh infeksi lentivirus pada sapi Bali. Pemerintah merekomendasikan vaksinasi sebagai langkah penting pengendalian di wilayah endemis. Penelitian ini bertujuan untuk mempelajari dinamika tanggap antibodi dengan melakukan kajian kohort sejalan dengan program vaksinasi JD di Desa Pematang Kabau oleh Dinas Peternakan dan Perikanan Kabupaten Sarolangun. Sera dari 36 sapi Bali pendatang, yang menerima dua dosis vaksin JD-Vet® dengan selang waktu 30 hari, dikoleksi pada hari 0, 44, dan 90. Antibodi spesifik kapsid virus JD (JDV) diukur menggunakan metode ELISA. Sebelum vaksinasi, 88,89% sapi menunjukkan hasil seronegatif, sedangkan 11,11% menunjukkan seropositif yang menandakan adanya paparan JDV terdahulu. Tidak ada reaksi membahayakan yang diamati pada sapi yang divaksinasi. Vaksinasi menurunkan titer antibodi secara signifikan pada sapi seropositif. Sebaliknya, 71,87% sapi seronegatif menunjukkan respons positif, meskipun hanya 40,63% yang mencapai tingkat seroprotektif pada hari ke-44. Persentase ini menurun secara signifikan menjadi 15,63% pada hari ke- 90, mengindikasikan durasi kekebalan yang relatif pendek. Temuan ini menggarisbawahi pentingnya mempertimbangkan status kekebalan pravaksinasi dan menegakkan pengendalian lalu-lintas sapi Bali. Meskipun vaksin JD-Vet® terbukti aman, namun mempertahankan kadar antibodi yang tinggi masih menjadi tantangan. Kajian lebih lanjut sangat diperlukan untuk mengoptimalkan strategi vaksinasi dan meningkatkan pengendalian JD pada sapi Bali.
THE POTENTIAL OF ADJUVANT AGAINST PRODUCTION OF ANTISTREPTOCOCCAL IMMUNOGLOBULIN Y (IGY) IN AQUACULTURE Rifky Rizkiantino; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Retno Damajanti Soejoedono; Okti Nadia Poetri; Wyanda Arnafia; Kris Damar Sasi; Dinda Reisinta
Jurnal Kedokteran Hewan Vol 14, No 3 (2020): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i3.16911

Abstract

This study was conducted to explore the potential of adjuvant for the production of immunoglobulin Y (IgY) as antistreptococcosis in layer chicken with mass production orientation. Enterococcus faecalis which causes streptococcosis in the red tilapia was selected as a candidateantigen. The production of immunoglobulin Y (IgY) was carried out on Isa Brown layer chickens and aged around 20 weeks. Furthermore, thechickens were grouped into four groups (A, B, C, and D groups), each consisting of three chickens based on the type of adjuvant, while twochickens were used as a control group. Each group was treated by giving MONTANIDE™ ISA 71R VG adjuvant (A), Freund's adjuvant (B), aluminum potassium sulphate adjuvant (KAl(SO4)2∙12H2O) concentration of 50 ppm in pH 7 (C), and only antigens without adjuvant (D). Chickens were kept for 35 days and each week was checked for presence the IgY antigen in the serum and egg yolk. Booster was conducted on 14th and 28th days of maintenance. The results showed that IgY in treatment group A was detected on day 28 in the serum and day 35 in the yolk. Whereas the treatment group B could be detected on day 35 in the serum. However, the IgY was not detected in the serum and yolk in C, D, and control groups until the end of the maintenance. Based on the results, it can be concluded that the appearance of IgY in serum and yolk in a relatively fast time is obtained in the combination of Enterococcus faecalis antigen with the emulsion of water-in-oil adjuvant (SEPPICMONTANIDE™ ISA 71R VG) compared to the other types of adjuvant that use in this study.
Gambaran Patologi Embrio Ayam yang Terinfeksi Avian orthoreovirus Isolat Lokal Raditya Pradana Putra; Sri Murtini; Okti Nadia Poetri
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 9, No 1 (2024): January 2024
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v9i1.2143

Abstract

Avian orthoreovirus (ARV) is widespread and found in almost every commercial poultry farm and other poultry species. Virus isolation from local isolates is necessary to obtain vaccine seeds that are homologous to viruses in the field.  The R&D Laboratory of PT Vaksindo Satwa Nusantara successfully isolated ARV from field cases. Inoculation of SPF Embryonated Chicken Eggs (ECE) is the protocol used to evaluate virus titer, especially to calculate the antigen titer of vaccine prototype candidates. The gross pathology of chicken embryos infected with ARV to evaluate virus growth has not been widely reported. This study aims to observe the gross pathology of chicken embryos infected with ARV to measure the virus titer using infectivity test such as the embryo infectious dose 50 (EID50). The virus suspension was diluted from 10-1 to 10-6 then dilutions of 10-4 to 10-6 were inoculated each into five ECE. Incubated ten days and observed for embryonic death. The observations found the embryonic death on day seven. Pathological changes in the embryos are stunted, thin feather, oedema, and less allantois fluid compared to control of uninfected embryo. The results of the embryos necropsy showed cardiac necrosis, greenish liver, and gelatinous exudate in the abdominal cavity.Keywords - Avian Orthoreovirus, Embryonated Chicken Eggs, Embryo Pathology, EID50.
Intervensi Dampak Penyakit Kaki Dan Mulut Berbasis Biaya Manfaat Chaerul Basri; Etih Sudarnika; Okti Nadia Poetri; Atsmarina Widyadhari; Cut Desna Apriana; R. Dikky Indrawan; Asep Rakhmat; M. Gumilang Pramuwidyatama
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 5 No 3 (2023): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.5.3.719-722

Abstract

Upaya pengendalian dan pemberantasan PMK di Indonesia jika dilaksanakan dengan program vaksinasi dengan kecepatan seperti yang berlangsung saat ini maka akan menyebabkan waktu pemberantasan yang lebih lama dan kerugian ekonomi yang besar. Perbaikan program vaksinasi dengan kecepatan yang ideal selain dapat memperpendek waktu pemberantasan juga akan memberikan dampak keuntungan ekonomi yang sangat besar. Berdasarkan hasil studi ini direkomendasikan agar upaya pengendalian dan pemberantasan PMK di Indonesia sebaiknya program yang dilaksanakan mengacu kepada skenario vaksinasi dengan kecepatan yang ideal. Dalam penyusunan dan pelaksaan program pengendalian di Indonesia dapat menggunakan program pengendalian yang dikembangkan di Filipina sebagai bahan pertimbangan karena sudah terbukti secara efektif berhasil memberantas PMK dari negara kepulauan seperti di Indonesia
Laporan Kasus: Studi Morfopatologi Demam Babi Afrika pada Babi di Bogor, Jawa Barat Rahayu Woro Wiranti; Dwi Utari Rahmiati; Aldo Yanuar Wuriyantara; Okti Nadia Poetri; Ekowati Handharyani
Jurnal Veteriner Vol 24 No 4 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.4.564

Abstract

Demam Babi Afrika atau African swine fever (ASF) adalah penyakit menular yang dapat terjadi pada ternak babi dan babi liar. Penyakit tersebut disebabkan oleh virus ASF. Gejala klinis sangat bervariasi dari kasus yang terjadi perakut, akut, subakut dan kronis tergantung pada virulensi virus. Kejadian ASF dapat menyebabkan angka kematian tinggi pada babi, penurunan kondisi sosial, dan ekonomi termasuk kontaminasi lingkungan. Berdasar beberapa pertimbangan masalah tersebut, maka akan sangat bermakna bila dilakukan studi morfopatologi dan klarifikasi terhadap kejadian ASF pada babi (Sus scrofa domesticus). Kajian saat ini dilaksanakan dengan melakukan prosedur nekropsi terhadap dua ekor babi umur empat bulan, yaitu dengan melakukan pemeriksaan kondisi tubuh dan organ-organ interna. Sampel organ diambil untuk pemeriksaan histopatologi yang selanjutnya diwarnai dengan hematoksilin-eosin (HE). Pemeriksaan lanjut dilakukan dengan metode polymerase chain reaction (PCR) menggunakan primer universal (p72 dan p54). Hasil pemeriksaan makroskopik menunjukkan bahwa beberapa bagian kulit mengalami erythema, terjadi splenitis hemoragi, enteritis hemoragi disertai dengan lymphadenopathy pada limfonodus mesenterika dan terjadi hemoragi pada meningen dan pembuluh darah di otak. Hasil pemeriksaan histopatologimenunjukkan lesi pada organ limforetikuler seperti limpa dan limfonodus mesenterika, yaitu radang limfositik disertai hemoragi. Pemeriksaan pada otak menunjukkan lesi radang limfositik disertai hemoragi pada selaput meningen. Hasil pemeriksaan molekuler dengan metode PCR memberikan konfirmasi bahwa penyakit disebabkan oleh virus ASF. Berdasarkan hasil pemeriksaan komprehensif secara makroskopik, mikroskopik dan evaluasi PCR dapat disimpulkan bahwa kedua ekor babi tersebut menderita infeksi ASF tipe akut.
COMPARATIVE ANALYSIS OF PROBING AND CONTRAST RADIOGRAPHY FOR GENDER DETERMINATION IN RETICULATED PYTHONS (Malayopython reticulatus) Tan, Zhi Yien; Poetri, Okti Nadia; Ulum, Mokhamad Fakhrul
BIOTROPIA Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.3.2278

Abstract

Article Highlights- Accurate gender identification supports effective breeding programs and ensures snakes' welfare.- This study emphasizes that probing is more reliable and efficient than contrast radiography for gender determination in reticulated pythons.- The probing method proved to have 100% accuracy in determining the gender of reticulated pythons, outperforming contrast radiography, which only achieved 75% accuracy. AbstractReticulated pythons, like most snakes, do not exhibit noticeable sexual differences, which makes gender determination necessary. Probing and contrast radiography were chosen as methods to determine sex and to compare their accuracy. Six reticulated pythons with body lengths of 128-167 cm, tail lengths of 5.4-9.6 cm, and body diameters of 1.4-3.4 cm were examined twice by probing and contrast radiography. The results showed that probing had 100% accuracy, with probe lengths ranging from 1.37-3.60 cm for male snakes and 0.3-0.76 cm for female snakes. Contrast radiography had 75% accuracy, with the hemipenis appearing as a white silhouette and female snakes appearing colorless. The accuracy rates highlight the effectiveness of probing over contrast radiography for reliable gender determination in reticulated pythons
Formulasi Sabun Cair Perak Nanopartikel dengan Penstabil Polyvinil Alcohol : Sabun Cair Perak Nanopartikel Sutriyo; Hanandi, Sharon; Putri, Kurnia Sari Setio; Poetri, Okti Nadia; Annisa, Syifa; Rahmasari, Ratika
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol. 13 No. 1 (2021): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (608.928 KB) | DOI: 10.35617/jfionline.v13i1.115

Abstract

According to Global Burden of Disease reported on 2019, about 1.53 million deaths caused by infectious diseases such as pneumonia and diarrhea. Triclosan is one of the active ingredient commonly used in antibacterial soap as one way to prevent the spread of infectious disease.. However, bacteria resistance against triclosan has been reported. Silver nanoparticles (AgNP) is an alternative antibacterial that potential to be used in liquid hand wash. However AgNp tend to aggregate during storage, thus stabilizer is needed This study aims to synthesize AgNP, formulate the liquid hand wash contain AgNP with polyvinil alcohol as stabilizer, and evaluate its effectiveness against Escherichia coli, Staphylococcus aureus, and Salmonella thypi. AgNP was prepared using the chemical reduction method between silver nitrate and sodium borohydride, followed by its characterization using UV-Vis spectrophotometer, TEM, PSA, and AAS. The physical characteristic of AgNp-liquid hand wash were also evaluated. Further, the antibacterial activity of AgNP-handwash was evaluated by phenol coefficient method. The peak of UV absorption spectrum of colloidal was found at 404.2 nm indicated the presence of AgNP. Ag content in AgNP colloidal was 38.405 mg/Kg ± 0,008. The spherical shape of AgNP was observed. The AgNP size was 65.1 nm with polydispersity index value of 0.543, and zeta potential value was -22.25 mV. The obtained AgNP-hand wash met the Indonesian standard criteria and was stable for 28 days. The best phenol coefficient value was obtained at formulation with addition of 30% AgNP (0.1 for S. typhi, 0.4 for E. coli, and 0.01 for S. aureus).
Formulasi Nasal Spray Anti-Influenza yang Mengandung Nanopartikel Perak : Nasal Spray Anti-Influenza Nanopartikel Perak Sutriyo; Wilbert Wylie; Kurnia Sari Setio Putri; Okti Nadia Poetri; Rahmasari, Ratika
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol. 13 No. 2 (2021): Jurnal Farmasi Indonesia
Publisher : Pengurus Pusat Ikatan Apoteker Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.44 KB) | DOI: 10.35617/jfionline.v13i2.124

Abstract

Influenza A virus is one of the most common causes of respiratory disease in the world. Even though, vaccines and anti-influenza virus are become the first line for therapy, but the mutation ability of influenza virus is able to cause several outbreaks in the world. Silver nanoparticles (AgNP) have been proven to exhibit antiviral activity; however, the use of AgNP in pharmaceutical products is still limited. In this study, we aimed to formulate nasal spray containing AgNP, to evaluate its physicochemical properties, and its antiviral activity toward H5N1 influenza A virus. AgNP were synthesized using chemical reduction method with polyvinyl alcohol as stabilizer, and further prepared into nasal spray product. Physicochemical properties and anti-hemagglutination activity of nasal spray were further evaluated. The nasal spray contained different size of AgNP (less and more than 50 nm) showed physical stability after 28 days storage. However, Anti-influenza evaluation of nasal spray contained AgNP less than 50 nm exhibited better anti-hemagglutination activity against influenza A virus.
Co-Authors Agustin Indrawati Aldo Yanuar Wuriyantara Anindita Sista Widyadhari Anindita Sista Widyadhari Annisa, Syifa Ari Rumekso Asep Rakhmat Atsmarina Widyadhari Cece Sumantri Chaerul Basri Cut Desna Apriana Dilas Pradana Dinda Reisinta Dwi Desmiyeni Putri Dwi Utari Rahmiati Eko Sugeng Pribadi Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Ferry Ardiawan Hadri Latif Hanandi, Sharon I Wayan T. Wibawan I wayan Teguh Wibawan koekoeh santoso Kris Damar Sasi Kurnia Sari Setio Putri Kurnia Sari Setio Putri, Kurnia Sari Setio La Jumadin La Jumadin M. Gumilang Pramuwidyatama Melani Wahyu Adiningsih Mokhamad Fakhrul Ulum, Mokhamad Ni Luh Putu Ika Mayasari Novera Nirmalasanti Nur Khusni Hidayanto Nurjumaatun Nurjumaatun R. Dikky Indrawan Raditya Pradana Putra Rahayu WoroWiranti Rahmasari, Ratika Rahmat Setya Adji Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Rifky Rizkiantino sri murtini . Surachmi Setiyaningsih Sutriyo Suwarny Ruhi Tan, Zhi Yien Trioso Purnawarman Wilbert Wylie Wyanda Arnafia