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Journal : Reaktor

Kinetics of Enzymatic Hydrolysis of Passion Fruit Peel using Cellulase in Bio-ethanol Production Megawati Megawati; Astrilia Damayanti; Radenrara Putri; Angga Pratama; Tsani Muftidar
Reaktor Volume 20 No.1 March 2020
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.444 KB) | DOI: 10.14710/reaktor.20.1.10-17

Abstract

This research aims to study the hydrolysis of passion fruit peel using cellulase and its evaluation for ethanol production. Passion fruit peel is a fruit processing waste that has not been utilized properly. Passion fruit peel contains holo-cellulose (64% w/w), which can be converted into ethanol through hydrolysis followed by fermentation. Hydrolysis using cellulase is more efficient and its fermentation using yeast to produce ethanol is common. The hydrolysis is carried out at various enzyme ratios (3, 5, 7, and 9% v/v) and at temperature 30 oC, material concentration 5 g/100 mL, pH 4-5, and shaking speed 160 rpm. The kinetics chosen were heterogeneous models; they were the fractal model by Valjamae and Kopelman. Before being hydrolyzed, the essential oil and pectin in passion fruit peel were extracted, because the compositions were quite high; the results were around 16.23 and 11.36% w/w, respectively. The effect of the enzyme ratio to the sugar concentration by hydrolysis is very significant. At 9 h, the glucose concentration reached 45.38, 51.86, 60.50, 66.00 g/L at various enzyme ratios of 3, 5, 7, 9% v/v. During the hydrolysis, the glucose concentration continues to increase and starts to decrease after 9 h. Hydrolyzate solution fermentation obtained from hydrolysis in various enzyme ratios showed consistent results; the higher the enzyme ratio and glucose, and the higher the ethanol will be (5.6, 6.8, 7.6, and 8.9% v/v). The kinetics model by Valjamae is more appropriate to describe the enzymatic hydrolysis mechanism of passion fruit peel than Kopelman. The fractal exponent values obtained from Valjamae and Kopelman models were 0.28 and 0.27. In Valjamae model, the enzyme ratio rises, from 3 to 9% v/v, the rate constant rises from 0.22 to 0.53 1/h. In Kopelman model, the rate constant rises too, from 0.21 to 0.51 1/h.Keywords: bio-ethanol; cellulase; enzymatic hydrolysis; fractal kinetic; passion fruit peel
Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads Astrilia - Damayanti; Sarto - Sarto; Wahyudi Budi Sediawan
Reaktor Volume 18 No. 2 June 2018
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.266 KB) | DOI: 10.14710/reaktor.18.2.57-62

Abstract

Rotten oranges contain glucose and limonene, in which limonene is an inhibitor of microorganisms. Immobilization of mixed culture used the entrapment method is the easiest method of protecting the mixed culture from inhibitors. Entrapment method with extrusion drip is an efficient and effective technique to produce beads. This study aims to determine the adsorption rate of adsorbate (glucose and limonene) on the adsorbent surface (beads). Materials used in this study were glucose, DL-limonene, mixed culture, and beads. Three types of beads consisted of alginate - no mixed culture (A), alginate and activated carbon - no mixed culture (CA), alginate and activated carbon - free mixed culture (CB). Adsorption column consist of 30 ml nutrient, 15 mL substrate, and 5 mL beads. If the beads do not contain mixed culture, nutrients and substrate were replaced by aquadest. The reactor was done in a batch system at 37oC. The lowest order of beads ability to adsorb glucose were AG followed by CAG and finally CBG, whereas to limonene solution were AL followed by CBL and finally CAL. Lagergren model was used to determined kinetic bioadsorption on limonene and glucose. The adsorption rate value in the pseudo-second order (k2,ad) for the glucose solution was ranged between 0.025 to 0.087 min-1, while the D-limonene was in the range between 2.084 to 5.233 min-1. Adsorption of glucose and limonene on the surface of the three types of adsorbents was reached steady state at the 60th minute.Keywords: orange, limonene, immobilization, adsorption, Lagergren model.
Co-Authors Ababil, Devara Candra Abdurrafi, Faishol Ahmed Tessario Ekanuramanta Amelia Fitri Ananda Muthi Athirah Angga Pratama Anggun Septiamurti Ar Rasyid, Moh. Rizal Artanti Putri, Dewi Ayasha Maharani Ayu Nur Permadhini Bahlawan, Zuhriyan Ash Shiddieqy Bariroh, Siti Bayu Triwibowo Catur Rini Widyastuti, Catur Rini Desy Hikmatul Siami Dewi Selvia Fardhyanti Doni Rahmat Wicakso Eko Setyawan Endah Ayu Fitriana Eva Amalia Alvionita Eva Amalia Alvionita Fadlurrohman, Fadlurrohman Fahreza Pracenda Felicitia Fitriana, Endah Ayu Fitriyani, Winda Risma Fortuna, Dwi Hanif Ardhiansyah Haniif Prasetiawan Harianingsih, Harianingsih Hernadin, Ivan Aldino Hutomo , Gayuh Aditya Hutomo, Gayuh Aditya Isaroyati, Luluk Kevin Thomas Khoridatus Sulwa Krisdayanti , Shendy kurniawan, Adhetya Larasati, Amalia Layliyah, Marifatul Luluk Arvi Cahyaning Suwandi Luqman Buchori M. Burhan Rubai Wijaya Maftuchan, Maftuchan Magfiroh, Meilina Maharani, Anggun Megawati Megawati - Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Melanie Hartalia Putrie Miftahuddin Azhari Noor Ubay, Isnina Nur Kholifah Chandra Mulyani Nur Kholifah Chandra Mulyani Nurkhasanah, Eva Nurul Huda Nuryoto Nuryoto Nuryoto Oktaviana, Vira Prasetyo, Ridwan Anung Prayogo, Robby Danang Qurnaini, Rika Alda Radenrara Dewi Artanti Putri Radenrara Dewi Artanti Putri, Radenrara Dewi Artanti Radenrara Putri Rahimsyah Ijas Nur Rasyid Rahmayetty Rahmayetty Revasha Ajeng Kamalia Reyhan Dwi Saputra Riana Defi Mahadji Putri Rizki, Muhammad Prabu Salma Alfahra Choirunisa Salwa, Dian Fatimatus Sandra Anggita Fadriana Sarto Sarto - Sarto Siti Bariroh Sulardjaka, S Syafitri, Laila Triani, Fita Tsani Muftidar Wahyudi Budi Sediawan Wahyudi Budi Sediawan Waryanto, Arif Wibowo, Muhammad Yusuf Widaya, Adi Risma Widi Astuti Widi Astuti Widi Widayat Wiguna, Taufan Satya Wijaya, Muhammad Burhan Rubai Winaningsih, Ima Yusuf Rumbino Zulfi Fitriani