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Journal : reaktor

Kinetics of Enzymatic Hydrolysis of Passion Fruit Peel using Cellulase in Bio-ethanol Production Megawati Megawati; Astrilia Damayanti; Radenrara Putri; Angga Pratama; Tsani Muftidar
Reaktor Volume 20 No.1 March 2020
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.444 KB) | DOI: 10.14710/reaktor.20.1.10-17

Abstract

This research aims to study the hydrolysis of passion fruit peel using cellulase and its evaluation for ethanol production. Passion fruit peel is a fruit processing waste that has not been utilized properly. Passion fruit peel contains holo-cellulose (64% w/w), which can be converted into ethanol through hydrolysis followed by fermentation. Hydrolysis using cellulase is more efficient and its fermentation using yeast to produce ethanol is common. The hydrolysis is carried out at various enzyme ratios (3, 5, 7, and 9% v/v) and at temperature 30 oC, material concentration 5 g/100 mL, pH 4-5, and shaking speed 160 rpm. The kinetics chosen were heterogeneous models; they were the fractal model by Valjamae and Kopelman. Before being hydrolyzed, the essential oil and pectin in passion fruit peel were extracted, because the compositions were quite high; the results were around 16.23 and 11.36% w/w, respectively. The effect of the enzyme ratio to the sugar concentration by hydrolysis is very significant. At 9 h, the glucose concentration reached 45.38, 51.86, 60.50, 66.00 g/L at various enzyme ratios of 3, 5, 7, 9% v/v. During the hydrolysis, the glucose concentration continues to increase and starts to decrease after 9 h. Hydrolyzate solution fermentation obtained from hydrolysis in various enzyme ratios showed consistent results; the higher the enzyme ratio and glucose, and the higher the ethanol will be (5.6, 6.8, 7.6, and 8.9% v/v). The kinetics model by Valjamae is more appropriate to describe the enzymatic hydrolysis mechanism of passion fruit peel than Kopelman. The fractal exponent values obtained from Valjamae and Kopelman models were 0.28 and 0.27. In Valjamae model, the enzyme ratio rises, from 3 to 9% v/v, the rate constant rises from 0.22 to 0.53 1/h. In Kopelman model, the rate constant rises too, from 0.21 to 0.51 1/h.Keywords: bio-ethanol; cellulase; enzymatic hydrolysis; fractal kinetic; passion fruit peel
Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads Astrilia - Damayanti; Sarto - Sarto; Wahyudi Budi Sediawan
Reaktor Volume 18 No. 2 June 2018
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.266 KB) | DOI: 10.14710/reaktor.18.2.57-62

Abstract

Rotten oranges contain glucose and limonene, in which limonene is an inhibitor of microorganisms. Immobilization of mixed culture used the entrapment method is the easiest method of protecting the mixed culture from inhibitors. Entrapment method with extrusion drip is an efficient and effective technique to produce beads. This study aims to determine the adsorption rate of adsorbate (glucose and limonene) on the adsorbent surface (beads). Materials used in this study were glucose, DL-limonene, mixed culture, and beads. Three types of beads consisted of alginate - no mixed culture (A), alginate and activated carbon - no mixed culture (CA), alginate and activated carbon - free mixed culture (CB). Adsorption column consist of 30 ml nutrient, 15 mL substrate, and 5 mL beads. If the beads do not contain mixed culture, nutrients and substrate were replaced by aquadest. The reactor was done in a batch system at 37oC. The lowest order of beads ability to adsorb glucose were AG followed by CAG and finally CBG, whereas to limonene solution were AL followed by CBL and finally CAL. Lagergren model was used to determined kinetic bioadsorption on limonene and glucose. The adsorption rate value in the pseudo-second order (k2,ad) for the glucose solution was ranged between 0.025 to 0.087 min-1, while the D-limonene was in the range between 2.084 to 5.233 min-1. Adsorption of glucose and limonene on the surface of the three types of adsorbents was reached steady state at the 60th minute.Keywords: orange, limonene, immobilization, adsorption, Lagergren model.
Optimization of Rice Bran Oil Bleaching via Carotenoid Adsorption onto Activated Carbon using Response Surface Methodology (RSM) Damayanti, Astrilia -; Harianingsih, Harianingsih; Bahlawan, zuhriyan Ash Shiddieqy; Dillah, Shohib Qomad; Dewi, Yasinda Verrel Dinara Shinka; Kristi, Silvia Yolanda; Prasetyo, Ridwan Anung
Reaktor Volume 23 No.2 August 2023
Publisher : Department of Chemical Engineering, Faculty of Engineering, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.23.2.53-61

Abstract

Rice bran oil (RBO) is a by-product of rice processing with a good fatty acid profile and high oxidative stability. This study aims to optimize the bleaching stage of the rice bran oil refining process to determine the minimum levels of carotenoids in RBO by using the Response Surface Methodology (RSM). This research used the Central Composite Design (CCD) experimental design with three variables: bleaching temperature (50, 80, 1100C), bleaching time (20, 40, 60 minutes), and activated carbon concentration (0.4, 0.8, 1.2%). As a result, the model was enhanced to explain the correlation of each variable and the different data variations. This study employed the Derringer method to determine the most optimal variable variation that produces the lowest carotene levels. This study found that the optimal carotene level was 4.87 mg/kg with an activated carbon concentration of 1.18%, temperature of 500C, and time of 53.155 minutes.
Co-Authors Ababil, Devara Candra Abdurrafi, Faishol Ahmed Tessario Ekanuramanta Amalia Larasati Amelia Fitri Ananda Muthi Athirah Anfita Aulia Sakinah Angga Pratama Anggun Enjelita Anggun Septiamurti Ar Rasyid, Moh. Rizal Arie Taveriyanto Artanti Putri, Dewi Ayasha Maharani Ayasha Maharani Ayu Nur Permadhini Bahlawan, Zuhriyan Ash Shiddieqy Bariroh, Siti Bayu Triwibowo Bayu Triwibowo Calista Arsanti Ramadhani Catur Rini Widyastuti, Catur Rini Cyeva Primahasta Desy Hikmatul Siami Dewi Selvia Fardhyanti Dewi, Yasinda Verrel Dinara Shinka Dillah, Shohib Qomad Doni Rahmat Wicakso Eka Sari, Eka Eko Setyawan Endah Ayu Fitriana Eva Amalia Alvionita Eva Amalia Alvionita Fadlurrohman Fadlurrohman Fahreza Pracenda Felicitia Fera Utnita Sari Fitri Nur Kayati Fitriana, Endah Ayu Fitriyani, Winda Risma Fortuna, Dwi Grandis Belva Ardana Hanif Ardhiansyah Haniif Prasetiawan Harianingsih, Harianingsih Harianingsih, Harianingsih Harsya Grezdipa Pratama Hernadin, Ivan Aldino Hutomo , Gayuh Aditya Hutomo, Gayuh Aditya Ima Winaningsih Ima Winaningsih Indrasukma Permanadewi Irene Nindita Pradnya Isaroyati, Luluk Kakalia Putri Auralita Karenina Hakim Kautsar Taqi Imanullah Kevin Thomas Khoridatus Sulwa Krisdayanti , Shendy Kristi, Silvia Yolanda kurniawan, Adhetya Larasati, Amalia Layliyah, Marifatul Luluk Arvi Cahyaning Suwandi Luqman Buchori Luvena Salsabila M Burhan Rubai Wijaya M. Burhan Rubai Wijaya Maftuchan, Maftuchan Magfiroh, Meilina Maharani, Anggun Maulida Zakia Megawati Megawati - Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Megawati Melanie Hartalia Putrie Miftahuddin Azhari Moh. Rizal Ar Rasyid Muhamad Reihan Prananda Muhammad Fadhlan Dzikri Muhammad Prabu Rizki Noor Ubay, Isnina Nur Kholifah Chandra Mulyani Nur Kholifah Chandra Mulyani Nurkhasanah, Eva Nurul Huda Nurul Khusna Nuryoto Nuryoto Nuryoto Oktaviana, Vira Prasetyo, Ridwan Anung Prayogo, Robby Danang Qurnaini, Rika Alda Radenrara Dewi Artanti Putri Radenrara Dewi Artanti Putri Radenrara Dewi Artanti Putri, Radenrara Dewi Artanti Radenrara Putri Rahimsyah Ijas Nur Rasyid Rahmayetty Rahmayetty Revasha Ajeng Kamalia Reyhan Dwi Saputra Riana Defi Mahadji Putri Ridwan Anung Prasetyo Salma Alfahra Choirunisa Salwa, Dian Fatimatus Sandra Anggita Fadriana Sangeetaprivya P. Siva Sarto Sarto - Sarto Sarto Sarto Serlia Fitri Sadan Siti Bariroh Sri Agustina Sulardjaka, S Syafitri, Laila Taufan Satya Wiguna Triani, Fita Tsani Muftidar Wahyudi Budi Sediawan Wahyudi Budi Sediawan Wahyudi Budi Sediawan Waryanto, Arif Wibowo, Muhammad Yusuf Widaya, Adi Risma Widi Astuti Widi Astuti Widi Widayat Widya Sari Kusuma Wijayanti Wijaya, Muhammad Burhan Rubai Yessy Anna Pratiwi Yusuf Rumbino Zulfi Fitriani