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Phytochemical Screening and Antioxidant Activity of Selekop (Lepisanthes amoena) Fruit Heriad Daud Salusu; Farida Ariani; Ernita Obeth; Mark Rayment; Edy Budiarso; Irawan Wijaya Kusuma; Enos Tangke Arung
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.810

Abstract

Selekop (Lepisanthes amoena (Hassk.) Leenh.) plant leaves are used by the Dayak tribe of East Kalimantan as traditional cosmetics. Selekop fruit is also edible, but not well known. This study was conducted to obtain the phytochemical content and antioxidant assay in flesh, seed and pericarp extracts from the fruit of Selekop. Phytochemical analysis was conducted on ethanol extract for identification of flavonoid, alkaloid, tannin, saponin, triterpenoid and steroid. The antioxidant activity was done by DPPH assay with ascorbic acid as positive control. The flesh contained flavonoid, saponin, and tannin; the seed contained flavonoid, alkaloid, saponin, triterpenoid, and tannin; and the pericarp contained flavonoid, alkaloid, saponin, triterpenoid, and tannin. Analysis of antioxidant activity revealed the following Inhibitory Concentration (IC50 values): 122.51 ppm of flesh, 63.30 ppm of seed, 53.21 ppm of pericarp and 3.06 ppm of ascorbic acid. Based on these results, the ethanol extract of the seed and the flesh had a phytochemical content and antioxidant activity which was better than the flesh extract from Selekop fuit.
Senyawa fenolik dan flavonoid, dan aktivitas antioksidan ekstrak metanol kulit buah ihau (Dimocarpus longan Lour. var. malesianus Leenh.) Nor Hikmah; Enos Tangke Arung; Sukemi Sukemi
Bivalen: Chemical Studies Journal Vol 3 No 2 (2020)
Publisher : Bachelor Degree Program of Chemical Education - Mulawarman University | Program Studi S-1 Pendidikan Kimia - Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bcsj.v3i2.447

Abstract

Senyawa fenolik dan flavanoid adalah senyawa kimia yang bertindak sebagai penangkal radikal bebas. Senyawa tersebut banyak terkandung dalam tanaman dan buah-buahan. Penelitian ini dirancang untuk menentukan total senyawa fenolik dan flavanoid, dan aktivitas antioksidan ekstrak metanol kulit buah ihau (Dimocarpus longan Lour. var. malesianus Leenh.). Serbuk kering kulit buah ihau dimaserasi dalam metanol selama 48 jam dan difiltrasi. Filtrat dipekatkan menggunakan evaporator. Total senyawa fenolik ditentukan menggunakan metode Follin Ciocalteau dan total flavanoid ditentukan menggunakan tes dengan AlCl3. Aktivitas atioksidan diukur menggunakan uji penangkapan radikal DPPH. Total senyawa fenolik adalah 383,720±0,986 mg/g ekstrak dan total senyawa flavanoid adalah 26,000±0,001 mg/g eksrak. Ekstrak menunjukkan aktivitas penangkapan radikal DPPH dengan pesentase penangkapan 85.6% pada konsentrasi 200 ppm. Hasil ini menunjukkan bahwa kulit buah ihau memeiliki potensi sebagai sumber agen penangkap radikal bebas.
Aktivitas Antioksidan Senyawa yang berasal dari Kayu Artocarpus heterophyllus ENOS TANGKE ARUNG; KUNIYOSHI SHIMIZU
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 2 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.381 KB)

Abstract

Isoprenoid-substituted flavonoids were isolated from the wood of Artocarpus heterophyllus bybiologically activity-guided fractionation, namely antioxidant. The isolated compounds are cudraflavoneC, kuwanon C, norartocarpin, albanin A and artocarpanone have more hydroxyl groups seem to bespecifically to scavenge H2O2. These experimental results demonstrated that each scavenging compounddifferent in its scavenge capacity toward different form of radicals.
Skrining Fitokimia, Antioksidan dan Aktivitas Antibakteri Ekstrak Etanol Akar Segar Bangle (Zingiber montanum) Amalia Riska Setyani; Enos Tangke Arung; Yanti Puspita Sari
Jurnal Riset Teknologi Industri Vol.15 No.2 Desember 2021
Publisher : Balai Riset dan Standardisasi Industri Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26578/jrti.v15i2.7436

Abstract

Bangle (Zingiber montanum) merupakan family zingiberaceae yang dimanfaatkan sebagai obat tradisional untuk mengobati beberapa penyakit. Tanaman bangle yang dikembangkan sebagai obat tradisonal tidak lepas dari kandungan senyawa aktif didalamnya. Kandungan senyawa aktif tanaman bangle memiliki efek aktivitas antioksidan dan antibakteri. Tujuan penelitian ini yaitu untuk mengetahui senyawa fitokimia, aktivitas antioksidan dan konsentrasi efektif ekstrak akar segar bangle (Z. montanum) dalam menghambat pertumbuhan bakteri Escerichia coli dan Streptococcus sobrinus. Penelitian ini menggunakan metode kolorimetri, DPPH dan kertas cakram (tes Kirby & Baurer). Sampel yang digunakan ekstrak etanol akar segar bangle dengan konsentrasi ekstrak 25%, 50%, 75%, 100%. Kontrol positif yaitu kloromfenicol dan kontrol negatif yaitu etanol 10%. Hasil penelitian menunjukkan bahwa ekstrak akar segar mengandung senyawa fitokimia berupa alkaloid, fenolik, flavonoid, saponin dan triterpenoid serta nilai TPC, TFC, dan antioksidan adalah 49.01 mg GAE/g, 394.07 mg CE/g dan 0.993 µg/mL. Aktivitas antibakteri memiliki daya hambat pada bakteri Escherichia coli dan Streptococcus sobrinus dengan konsentrasi 100% secara berturut-turut adalah 16.18 mm dan 9.86 mm.   Kata Kunci: akar segar bangle, aktivitas antioksidan, antibakteri, uji fitokimia ABSTRACT Bangle (Zingiber montanum) is the Zingiberaceae family that is used as a traditional medicine to treat some diseases. The Bangle plant developed as a traditional medicine cannot be separated from the active compounds contained in it. The active compound content of the bangle plant has the effect of antioxidant and antibacterial activity. The purpose of this study was to determine the phytochemical compounds, antioxidant activity and effective concentration of bangle fresh root extract (Z. montanum) in inhibiting the growth of Escerichia coli and Streptococcus sobrinus bacteria. This research used colorimetric method, DPPH and disc paper (Kirby & Baurer test). The sample used ethanol extract of bangle fresh root with extract concentrations of 25%, 50%, 75%, 100%. The positive control was chloramphenicol and the negative control was 10% ethanol. The results showed that the fresh root extract contained phytochemical compounds in the form of alkaloids, phenolics, flavonoids, saponins and triterpenoids and the TPC, TFC, and antioxidant values were 49.01 mg GAE/gr, 394.07 mg CE/gr, and 0.993 g/mL. Antibacterial activity had inhibitory activity in Escherichia coli and Streptococcus sobrinus bacteria with a concentration of 100%, respectively 16.18 mm and 9.86 mm. Keywords: bangle fresh root, antioxidant activity, antibacterial, phytochemical screening
Uji aktivitas antibakteri ekstrak propolis lebah Heterotrigona itama dari beberapa lokasi budidaya di Kalimantan Timur terhadap bakteri Propionibacterium acnes Enos Tangke Arung; Sarah Azzara Dikarulin; Dyah Ayu Dewi Listyaningrum; Bayu Susetya Ananda; Tasya Ananda Putri; Rudianto Amirta; Yuliansyah Yuliansyah; Irawan Wijaya Kusuma; Rico Ramadhan; Enih Rosamah; Wiwin Suwinarti
ULIN: Jurnal Hutan Tropis Vol 6, No 2 (2022)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v6i2.7824

Abstract

Propolis merupakan produk penting bagi lebah yang banyak digunakan sebagai salah satu alternatif pengobatan alami karena mengandung senyawa bioaktif yang dapat memberikan efek positif pada tubuh. Sebagai produk yang dihasilkan lebah untuk menutupi sarangnya dan mencegah predator maupun infeksi bakteri atau virus dari luar, propolis memiliki salah satu manfaat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak propolis lebah Heterotrigona itama yang berasal dari 4 lokasi budidaya (perkebunan, persawahan, hutan sekunder, dan perkotaan) di Kalimantan Timur terhadap bakteri Propionibacterium acnes atau P.acnes dengan metode difusi lubang/sumuran pada media nutrient broth (NB) dengan kloramfenikol sebagai kontrol positif dan aseton sebagai kontrol negatif. Propolis diekstrak menggunakan metode maserasi dengan larutan etanol 96% yang kemudian dibuat dalam 3 (tiga) konsentrasi yaitu 500ppm, 250 ppm, dan 125 ppm. Hasil penelitian menunjukanfitokimia dari propolis di 4 lokasi budidaya dominan mengandung alkaloid, flavonoid dan kumarin. Uji bakteri pada konsentrasi 500 ppm,propolis H.itama dari lokasi perkebunan dan persawahan tergolong dalam kategori kuat dalam menghambat pertumbuhan bakteri, dari lokasi hutan sekunder tergolong sedang, dan dari lokasi perkotaan tergolong rendah.
Evaluations of Antibacterial Properties of Zingiber purpureum Essential Oil Against 13 Different Gram-positive and Gram-negative Bacteria Nataniel Tandirogang; Silvia Anitasari; Enos Tangke Arung; Swandari Paramita; Yung Kang Shen
The Indonesian Biomedical Journal Vol 14, No 3 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i3.1967

Abstract

BACKGROUND: Indonesia's tropical forest is home to around 80% of the world's medicinal plants. One of these is Zingiber purpureum, which have traditionally been used to treat joint discomfort, the common cold, and jaundice. The rhizomes of this plant have been suggested to possess antibacterial action in the treatment of infections. In this study, Z. purpureum was screened for antibacterial activity against 13 bacteria (Gram-positive and Gram-negative).METHODS: Z. purpureum rhizomes were obtained and the distillated extracts were made to generate essential oil. The minimum inhibitory concentration (MIC) and Kirby Bauer disk diffusion methods were used to determine the antibacterial activity.RESULTS: All bacteria activity were inhibited by the essential oil of Z. purpureum at concentrations ranging from 2.5 vol% to 10 vol%. However, several bacterias (Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae) were inhibited at the lowest concentration (0.63 vol %), with the inhibition zones ranging from 6.7 mm to 8.0 mm. Meanwhile, the widest inhibition zone (13.3 mm) was reported on E. cloacae at 10 vol% concentration.CONCLUSION: A 10 vol% Z. purpureum rhizome extract inhibits Gram-positive and Gram-negative bacteria, particularly those that are resistant to a variety of antibiotics.KEYWORDS: Zingiber purpureum, antibacterial agents, susceptibility test, infection
The use of animal manure for improving chemical properties of degraded Ultisol, yield, and secondary metabolic of Zingiber montanum Nurul Puspita Palupi; Roro Kesumaningwati; Subeki Subeki; Kadis Mujiono; Sofian Sofian; Swandari Paramita; Enos Tangke Arung
Journal of Degraded and Mining Lands Management Vol. 11 No. 1 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2023.111.4847

Abstract

Ultisols in Indonesia have the potential for agricultural development, but the soils have low pH and nutrient contents that hinder plant growth and yield. Using animal manure can be an alternative to improve soil productivity and crop yields. This study aimed to examine the effects of animal manure on the chemical properties of Ultisol, yield, and secondary metabolic of Zingiber montanum. The treatments tested were combinations of types of manure (cow and chicken manure) and manure application levels, namely P0 (control), P1 (cow manure 20 t/ha), P2 (cow manure 40 t/ha), P3 (cow manure 60 t/ha), P4 (chicken manure 20 t/ha), P5 (chicken manure 40 t/ha), and P6 (chicken manure 60 t/ha). The results showed that the application of chicken manure of 60 t/ha increased N and P contents of the soil, and the application of cow manure of 60 t/ha increased soil cation exchange capacity. The application of cow manure of 60 t/ha gave the highest plant height, the number of leaves, and the number of at 18 weeks after planting, while the application of chicken manure dose of 60 t/ha produced the longest plant roots. The highest fresh and dry rhizome weight was observed for the 60 t/ha cow manure treatment. The highest secondary metabolic levels in each parameter were found in dry rhizomes (phenolic, flavonoid, and tannin) and fresh rhizomes (phenolic and flavonoid), with the highest tannin compound in the treatment of 40 t chicken manure/ha. The application of chicken manure at a dose of 60 t/ha resulted in a strong antioxidant yield in fresh and dry rhizomes.
A Dermatological Safety Test of a Face Serum Formulation Derived from Honey and Propolis of Stingless Bee from East Kalimantan Dewi, Januartha Kisari; Toruan, Vera Madonna Lumban; Paramita, Swandari; Arung, Enos Tangke
International Journal of Multidisciplinary: Applied Business and Education Research Vol. 5 No. 3 (2024): International Journal of Multidisciplinary: Applied Business and Education Rese
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ijmaber.05.03.01

Abstract

Skincare, which is a type of cosmetics, is an essential component in the process of maintaining and caring for the skin. It is known that the skin quickly absorbs the tiny molecular structure of serum. Stingless bees, widespread in East Kalimantan and Indonesia, benefit the skin's health by producing honey and propolis. This research aims to demonstrate that a face serum derived from honey and propolis of stingless bees from East Kalimantan is safe for use on the skin using dermatological standards. Here we will see whether there are allergic reactions and irritation from using facial serum. Using the Repeated Open Application Test (ROPT) technique, 20 volunteers were subjected to applying a face serum containing honey and stingless bee propolis. The serum was given to the upper arm twice daily for seven days. As part of the test, allergic reactions were evaluated using the system developed by the International Contact Dermatitis Research Group (ICDRG), and irritating reactions were evaluated using the Primary Irritation Index (PII). The results showed that on the first day, four of the volunteers reported moderate irritation reactions. Consequently, they scored 0.7, indicating they suffered minor irritation. On the other hand, the reactions of the remaining 16 volunteers were typical. Twenty participants had normal skin from the second treatment until the seventh day. The overall findings show that the facial serum based on honey and kelulut propolis from stingless bees from East Kalimantan showed a mild irritation reaction at the beginning of use and was safe to use in subsequent uses.
Kandungan fitokimia dan aktivitas antibakteri madu Heterotrigona itama yang diternakkan pada areal tumbuhan caliandra (Calliandra culothyrsus) terhadap bakteri Escherichia coli Arung, Enos Tangke; Ardy, Ari; Syafrizal, Syafrizal; Naibaho, Netty; Paramita, Swandari
ULIN: Jurnal Hutan Tropis Vol 8, No 1 (2024)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/ujht.v8i1.12967

Abstract

Madu Heterotrigona itama dikenal sebagai alternatif dalam pengobatan yang sering dijumpai dengan langsung dikonsumsi. Kemampuan madu dalam menghambat aktivitas bakteri karena mengandung senyawa antiseptik. Penelitian ini bertujuan untuk mengeksplorasi potensi  madu lebah tanpa sengat (kelulut) Heterotrigona itama yang diternakkan pada areal tumbuhan kaliandra dalam menghambat aktivitas bakteri Escherichia coli selama 6 bulan pengamatan (Oktober 2022 – Maret 2023). Penelitian ini menggunakan metode difusi agar untuk menguji sifat antibakteri madu. Prosedur ini meliputi penuangan media nutrient agar ke cawan petri dan dibiarkan mengering. Kemudian, dibuat lubang pada media dan ditambahkan suspensi bakteri. Dilakukan pengujian pada sampel secara intensif selama enam bulan. Kloramfenikol digunakan sebagai kontrol positif dan aquades sebagai kontrol negatif. Hasil menunjukkan bahwa  madu lebah tanpa sengat Heterotrigona itama efektif dalam menghambat pertumbuhan  bakteri Escherichia coli. Sifat antibakteri madu dipengaruhi oleh efek senyawa bioaktif yang dominan.
Antioxidant Assay of the Ethanolic Extract of Three Species of Rattan Fruits using DPPH Method Heriad Daud Salusu; Farida Aryani; Edy Budiarso; Irawan Wijaya Kusuma; Enos Tangke Arung; Abdul Rasyid Zarta
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 4 (2018): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i4.170

Abstract

The bioactivity of some species of rattan fruit has been previously studied and it was found that it has potential antioxidant agents. This study was conducted to determine the antioxidant content in three species of edible rattan fruit, namely Calamus manan Miq., Calamus ornatus Bl. and Calamus caesius Bl by using the DPPH method, whereby the test was carried out on the pericarp, flesh, and seeds of each fruit. The research findings revealed that phytochemical compounds, i.e. flavonoids, tannins, and triterpenoids are found almost in all parts of the fruit, while steroid is not found in any of the three species of the fruit. The antioxidant activity found in the fruit of C. caesius Bl is strong, meanwhile the antioxidant activity in the fruit of C. manan Miq only strong in its pericarp and seeds, but it is moderate in its flesh. The antioxidant activity found in the fruit of C. ornatus Bl. is strong in its pericarp and seeds, but it is moderate in its flesh. Therefore, these three species of rattan fruit are potential as antioxidant agents.