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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Prosiding Seminar Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Majalah Obat Tradisional Jurnal Teknologi Laboratorium JMM (Jurnal Masyarakat Mandiri) Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika Gorontalo Journal of Public Health Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Borneo Journal of Medical Laboratory Technology Narra J Quality : Jurnal Kesehatan Sureq: Jurnal Pengabdian Masyarakat Berbasis Seni dan Desain Prosiding University Research Colloquium Media Kesehatan Politeknik Kesehatan Makassar Jurnal Kefarmasian Indonesia
Sri Darmawati
Muhammadiyah Semarang University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : borneo journal of medical laboratory technology

 1 
Imunogenesitas Protein Pilli Salmonella Typhi Pada Mencit Balb/C Dengan Mengukur Kadar TNF-α: Imunogenesity Of Protein Pilli Salmonella typhi in BALB/c Mice by Measuring TNF-α Controls Hidayani, Al; Darmawati, Sri
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6074

Abstract

Typhoid fever, often called typhoid disease, is caused by Salmonella enterica subspecies enterica serevoar typhi (Salmonella typhi). The virulence of S.typhi bacteria depends on its ability to invade host cells consisting of several components such as cell wall antigens, flagellum antigens, Vi antigens and pilli antigens. Pilli is one of the virulence factors of S.typhi bacteria, which functions as a tool for adhesion or interaction between bacteria. The study was conducted to determine how the immunogenicity of pilli protein of S.typhi bacteria isolate BA07.4 was immunized in Balb/C mice at varying doses (2-6μg) by measuring TNF- α. The analysis carried out obtained the optimum results of TNF-α levels at a dose of 3μg with an average level of 62.152 pg / ml. In the research conducted, it was found that S.typhi pilli protein can induce specific antibodies against pilli antigens. This shows that the pilli protein is immunogenic, can cause an immune response and can induce antibodies that can recognize the immunized pilli protein sub-unit.
Karakterisasi Imunogenesitas Protein Pilli Salmonella Typhi: Analisis Absorban Antibodi dan Spesifisitas dengan Western Blot pada Mencit Balb/C Hidayani, Al; Darmawati, Sri; Ramdhani , Fitria Hariati; Rahmah, Windya Nazmatur
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 2 (2026): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i2.12477

Abstract

Protein pili berperan dalam adhesi dan bersifat imunogenik, berpotensi sebagai kandidat vaksin subunit. Penelitian ini menganalisis imunogenesitas protein pili S. typhi isolat BA07.4 melalui pengukuran absorbansi antibodi dan karakterisasi spesifisitasnya. Protein pili diisolasi dengan metode biphasic, dimurnikan, dan digunakan untuk imunisasi 30 ekor mencit Balb/C (6 kelompok: kontrol dan dosis 2, 3, 4, 5, 6 µg) secara intraperitoneal tiga kali. Kadar antibodi diukur dengan inhouse ELISA (λ 405 nm) dan spesifisitasnya dianalisis dengan Western blot. Rerata absorbansi tertinggi pada dosis 5 µg (2,827), diikuti dosis 6 µg (2,678), 4 µg (2,456), 3 µg (2,134), 2 µg (1,856), dan kontrol (0,423). Analisis One Way ANOVA menunjukkan perbedaan signifikan antar kelompok (p=0,000) dengan semua kelompok perlakuan berbeda signifikan terhadap kontrol (p<0,05). Western blot menunjukkan pita spesifik pada berat molekul 87, 58, dan 42 kDa pada kelompok dosis 5 µg, sementara kontrol tidak menunjukkan pita. Protein pili S. typhi bersifat imunogenik dengan dosis optimum 5 µg, menghasilkan antibodi yang secara spesifik mengenali sub unit protein pili 87, 58, dan 42 kDa, mendukung potensinya sebagai kandidat vaksin subunit.
Co-Authors A.A. Ketut Agung Cahyawan W Afia, selfa Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Ainutajriani, Ainutajriani Al Ansyari, Mas’ud Uqbah Ana Hidayati Mukaromah Anak Agung Wiradewi Lestari Aprilia Indra Kartika, Aprilia Indra Arif Mulyanto, Arif Arisyi, Dani Fajrul Arya Iswara Batara, Murni Budi Santosa Budi Santosa BUDI SETIADI DARYONO Cahyaningrum, Dea Galuh Daryono, Budi S. Dewi Seswita Zilda Edward Kurnia Setiawan Limijadi, Edward Kurnia Setiawan Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Ethica, Stalis N. Faisal Faisal Fandhi Adi Wardoyo Fariza Yulia Kartika Sari Felim, Jethro Fuad, Hayatun Geleta, Daniel Hariutami, Desi Hattah, Muh. Afif Novaldy Hidayani, Al Hikmah, Annisa Nurul Inayah, Faidhul Jannah, Erni M Joharlinda, Joharlinda La’lang, Marselaonety LANGKAH SEMBIRING Lia Kusmita Listiyanti, Apriliya Manufandu, Anjar Richardo Mardiyah Hayati Masashi Kawaichi, Masashi Maulida, Gita Rossa NANIK RAHMANI Naqib, Ahmad Nasruddin, N Nur Hidayah Palimbongan, Junita Rensa Permana, Atna Prastiyanto, Muhammad E. Prastiyanto, Muhammad Evy Prastiyanto, Muhammad Evy Putri, Desty Ratna Putri, Wahyu Aristyaning Ragil Saptaningtyas, Ragil Ramdhani , Fitria Hariati Retnaningrum, Endah Rinaldi, Muhamad Rizqi Rizal Maarif Rukmana, Rizal Maarif Rosnita . Rustiyarso . Sah, Shiv Kumar Salsabila, Nia Sa’adah, Arina Lis Setianingsih, Erna Silvestre, Richard David Sri Sinto Dewi Stalis Norma Ethica Sukoharjanti, Bintari Tri Sulistyaningtyas, Ayu Rahmawati Susanti, A.Meryam Syaiful Anwar Trianes, Juwy Tulus Ariyadi Wayan T. Artama Wayan T. Artama Widya Asmara Wilson, Wildiani Windya Nazmatur Rahmah Zilda, Dewi S. Zuraida Zuraida