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Journal : AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment)

Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Anak Agung Sagung Putri Risa Andriani; Widyatmani Sih Dewi; Novizar Nazir; Ni Luh Putu Putri Setianingsih; Eko Rini Indrayatie; Krisnawan Kalimutu
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

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Abstract

The characteristics of indigenous bacterial isolates in cocoa farms differ based on the study's location and methodology. More research is required to comprehend these bacterial isolates' diversity and features completely. This study intends to assess the cellulose-degrading capacity of bacterial isolates from cocoa plantations in Meko Village, Central Sulawesi. The shape of the bacterial colony, the shape of the colony's edge, elevation, colony size, color, and texture are considered during macroscopic morphological identification and microscopic morphological identification after gram stain. Afterward, gram stains the bacteria to determine the type of cell wall. Hydrolysis capacity was then identified macroscopically by observing colony shape, colony periphery, colony color, colony appearance and height, and hydrolysis capacity on media in a petri dish (HC). Upon microscopic examination of cell shape and bacterial wall type (Gram-positive and gram-negative). Based on the research findings and identification of bacteria capable of degrading cellulose in cocoa soil, it can be inferred that among the 28 examined isolates, six isolates have the greatest HC index values in KL62 isolates. In contrast, GL66, KL23, and KL62x isolates have medium HC index values.
Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Anak Agung Sagung Putri Risa Andriani; Widyatmani Sih Dewi; Novizar Nazir; Ni Luh Putu Putri Setianingsih; Eko Rini Indrayatie; Krisnawan Kalimutu
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The characteristics of indigenous bacterial isolates in cocoa farms differ based on the study's location and methodology. More research is required to comprehend these bacterial isolates' diversity and features completely. This study intends to assess the cellulose-degrading capacity of bacterial isolates from cocoa plantations in Meko Village, Central Sulawesi. The shape of the bacterial colony, the shape of the colony's edge, elevation, colony size, color, and texture are considered during macroscopic morphological identification and microscopic morphological identification after gram stain. Afterward, gram stains the bacteria to determine the type of cell wall. Hydrolysis capacity was then identified macroscopically by observing colony shape, colony periphery, colony color, colony appearance and height, and hydrolysis capacity on media in a petri dish (HC). Upon microscopic examination of cell shape and bacterial wall type (Gram-positive and gram-negative). Based on the research findings and identification of bacteria capable of degrading cellulose in cocoa soil, it can be inferred that among the 28 examined isolates, six isolates have the greatest HC index values in KL62 isolates. In contrast, GL66, KL23, and KL62x isolates have medium HC index values.
Co-Authors Achmad Adi Surya Sustama Ahmad Yunus Aisyah Defara Rahmadani AKTAVIA HERAWATI Anak Agung Sagung Putri Risa Andriani Anggita, Akas Arlindo Fernando Macie Arwa Farida Lukito Atmanto Heru Wibowo Atmanto, Heru Bambang Pujiasmanto Canggih Jati Nusantara Canggih Jati Nusantara, Canggih Jati Desti Dian Amalina Dwi Priyo Ariyanto Dwi Priyo Ariyanto Eko Rini Indrayatie Farida Yuliani Ferina Pungky Ganjar Herdiansyah Ganjar Herdiansyah Ganjar Herdiansyah, Ganjar Hardian, Tiara Hasanah, Khalyfah Herwin Setyaningsih Hery Widijanto Ika Ernawati Indratin Indratin Indratin Indratin Irmawati, Viviana Istiqomah, Nanda Mei Jauhari Syamsiyah Jauhari Syamsiyah Komariah K Komariah Komariah Komariah Komariah Krisnawan Kalimutu Kurniatun Hairiah Muhammad Rizky Romadhon Mujiyo Mujiyo Ni Luh Putu Putri Setianingsih Noorcahyati Noorcahyati Novizar Nazir Prabang Setyono Purnomo Edi Sasongko Purwanto Purwanto Purwanto Purwanto PUTRI HANDAYANI Putri Permatasari Rahayu Rahayu Rahayu Rahayu Rahayu Rahayu Ramdan Hidayat Romadhon, Muhammad Rizky Sapja Anantanyu Sri Hartati Sri Rossati Sri Rossati Sri Wahyuni Sri Wahyuni Sudadi Sudadi Sudadi Sudadi Sudjono Utomo Sulandjari Sulandjari Sumani Sumani Sumani Sumani Sumarno . Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Suntoro Suntoro Suntoro Suntoro Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Suryono Suryono Suryono Suryono Sutarno Sutarno Usman Siswanto Vita Ratri Cahyani Wahyu Adi Nugroho Wahyu Galang Pranata Yoviana Erdhika Adiarti