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The Study of Artemisinin Content in Callus Artemisia annua L. Cultures Elicited with Endophytic Fungi Aspergillus sp. Farida Yuliani; Widyatmani Sih Dewi; Ahmad Yunus; Usman Siswanto
Molekul Vol 13, No 2 (2018)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.067 KB) | DOI: 10.20884/1.jm.2018.13.2.459

Abstract

Artemisinin, is a sesquiterpene lactone which is originally extracted from the medicinal plant Artemisia annua L. Artemisinin is a very effective antimalarial agent, however it is produced in very low amounts in plants. Chemical synthesis of artemisinin is complicated and not economically feasible. Researchers often use elicitation methods to increase artemisinin content in a laboratory scale with biotic or abiotic elicitor. This study examined the effect of elicitation using endophytic fungi Aspergillus sp on artemisinin content of callus A. annua cultures. Observations were made on its growth, texture and color, chlorophyll content and dry weight. Fungi were isolated from the stem tissue of A. annua and callus were grown on Murashige and Skoog medium. After 3 weeks of incubation, callus treated with four different elicitors concentration (0, 2, 4, 6 mg/mL). There were significant alterations on callus culture in terms of growth, texture, color and chlorophyll content after elicitation. The results clearly demonstrated that the endophytic fungal elicitor Aspergillus sp could increase artemisinin content but did not affect the callus dry weight of A. annua. The treatment of 4 mg/mL  elicitor produced the highest artemisinin content, that increased 7.6 times compared to the control, from 0.0036% to 0.0275%.
Alih Teknologi Budidaya Lada Organik Sebagai Pagar Hidup Kepada Masyarakat Desa Kebonagung, Sidoharjo, Wonogiri Widyatmani Sih Dewi; Mujiyo Mujiyo; Rahayu Rahayu
PRIMA: Journal of Community Empowering and Services Vol 1, No 2 (2017): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/prima.v1i2.35151

Abstract

Masyarakat desa Kebonagung, Kec.Sidoharjo, Kabupaten Wonogiri mempunyai visi ingin mengembangkan desanya menjadi desa agrowisata organik terpadu. Saat ini, di desa Kebonagung telah dikembangkan padi organik dan buah naga organik. Masyarakat ingin mengembangkan tanaman lada secara organik, baik sebagai tanaman pagar maupun di tanam di lahan pekarangan mereka, karena lada bernilai ekonomi tinggi. Permasalahan yang dihadapi adalah mereka belum mengetahui teknologi budidaya lada secara organik, dan juga belum mengetahui apakah lahannya sesuai untuk untuk budidaya lada atau tidak. Kegiatan pengabdian ini bermitra dengan: (1) Asosiasi Pertanian Organik “Wono Agung” (APOW) dan (2) Kelompok Tani Sedya Maju. Kedua mitra berlokasi di desa Kebonagung.Tujuan dari program ini adalah: (1) alih teknologi dari perguruan tinggi kepada mitra tentang budidaya tanaman lada secara organik sehingga mitra mengembangkan lada secara organik dengan teknologi penanaman secara benar, maupun (2) memberikan informasi ilmiah tentang kesesuaian lahan di Kebonagung untuk budidaya tanaman lada. Metode yang digunakan meliputi: focus group disccusion (FGD), penyuluhan, praktik budidaya lada organik, dan pemantauan. Hasil menunjukkan bahwa mitra begitu antusias dalam menerima teknologi yang dibagikan karena memiliki pengharapan lada mempunyai nilai jual yang tinggi, dan mereka juga segera mempraktikkan budaya lada dari bibit yang dibagikan secara cuma-cuma. Berdasarkan hasil kajian ilmiah, desa Kebonagung sesuai untuk budidaya lada organik dengan pengelolaan tertentu.
ASOSIASI AKAR KUNING (Fibraurea tinctoria Lour.) DENGAN TUMBUHAN BERPOTENSI OBAT DI SAMBOJA, KALIMANTAN TIMUR Noorcahyati Noorcahyati; Sulandjari Sulandjari; Widyatmani Sih Dewi
Jurnal Hutan Tropis Vol 4, No 3 (2016): Jurnal Hutan Tropis Volume 4 Nomer 3 Edisi November 2016
Publisher : Lambung Mangkurat University-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jht.v4i3.4063

Abstract

Akar kuning (Fibraurea tinctoria Lour.) Is a medicinal plant at Kalimantan that has the potential of development and require conservation efforts. This research was aimed to observe the ecology, association and other plants that have the potential as medicinal plants in the community garden in Sungai Merdeka, Samboja East Kalimantan. Data collection were using purposive sampling plot of 20 x 20 m. Found 28 species of 21 families among the sites of various stages of growth. The highest IVI value at every growth is Fibraurea tinctoria with an IVI of 78.24 (seedlings), Archidendron jiringa with IVI 35.20 (saplings), Glochidion sp. IVI 109.94 (poles) and Artocarpus integer IVIs 109.94 (trees). The strongest associations at the level of the tree is Artocarpus integer. At the root of the akar kuning habitat obtained 19 species of plants that also have potential as a drug that can be developed with agroforestry system.Akar kuning (Fibraurea tinctoria) merupakan biofarmaka Kalimantan yang perlu mendapat perhatian untuk dikembangkan dan dilestarikan. Penelitian bertujuan untuk mendapatkan informasi ekologi, asosiasi dan tumbuhan lain yang berpotensi sebagai obat pada habitat akar kuning di kebun masyarakat di Desa Sungai Merdeka, Samboja Kalimantan Timur. Pengumpulan data dilakukan secara sengaja dengan teknik sampling kuadrat ukuran petak 20 x 20 m. Ditemukan 28 jenis dari 21 famili pada lokasi penelitian dari berbagai tingkat pertumbuhan. Nilai INP tertinggi pada setiap pertumbuhan adalah Fibraurea tinctoria dengan INP sebesar 78,24 (tingkat semai), jenis Archidendron jiringa dengan INP 35,20 (tingkat pancang), Glochidion sp. INP 109,94 (tingkat tiang) dan Artocarpus integer memiliki INP 109,94 (tingkat pohon). Asosiasi terkuat pada tingkat pohon adalah Artocarpus integer. Pada habitat akar kuning diperoleh 19 jenis tumbuhan yang juga memiliki potensi sebagai obat yang dapat dikembangkan secara bersama-sama.
Assessment of soil fertility using the soil fertility index method on several land uses in Tutur District, Pasuruan Regency of East Java Purnomo Edi Sasongko; Purwanto Purwanto; Widyatmani Sih Dewi; Ramdan Hidayat
Journal of Degraded and Mining Lands Management Vol 10, No 1 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2022.101.3787

Abstract

Changes in land use have an impact on the level of soil fertility. Soil management, such as land cover systems and the environment, are supporting factors. Assessment of soil fertility index using indicators of soil chemical properties such as organic C, availability of nutrients nitrogen (N), phosphorus (P), cation exchange capacity (CEC), and exchangeable cations (K, Ca, Mg, Na). The research was conducted in Tutur District, Pasuruan Regency, with a survey of 5 land uses, i.e., mixed crops, coffee plantations, apple plantations, vegetable fields, secondary forests, and conservation forests. Observations were made on elevation, slope, and vegetation. Parameters of physical properties measured were soil texture and aggregate stability. Observations of chemical properties included organic C, N, P, CEC, exchangeable cation (K, Ca, Mg, Na), and soil pH. Soil Fertility Index (SFI) data were analyzed using the Least Significant Difference method at a 5% level. The results showed the diversity of soil fertility levels obtained from the calculation of the soil fertility index. The soil fertility index value in 5 land uses in Tutur District ranged from 0.67 to 1.00. Coffee plantations had the highest SFI score of 1.00; this condition was supported by an organic C indicator of 6.21% and a CEC value of 39.12 cmol kg-1. Conservation forest is the land use with the lowest SFI value of 0.67. The value of CEC and cation exchange in conservation forests are factors that impact the low value of SFI. 
RESPON CACING PENGGALI TANAH Ponthoscolex Corethrurus TERHADAP BERBAGAI KUALITAS SERESAH Herwin Setyaningsih; Kurniatun Hairiah; Widyatmani Sih Dewi
Jurnal Tanah dan Sumberdaya Lahan Vol. 1 No. 2 (2014)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.213 KB)

Abstract

Forest conversion to agriculture systems leads to change on litter input (quantitatively and qualitatively) resulting lower diversity, population density and biomass of earthworm. Litter with ratio C/N <20, or ratio of (lignin (L)+polifenol (P))/N <10 classified as high quality which decomposed rapidly. Six types of litter application were tested i.e. prunning of cofee with the lowest (L+P)/N of 7.5, Gliricidia (L+P)/N of 10, avocado with (L+P)/N of 31, Cofee+Gliricidia with (L+P)/N of 12, Cofee+Gliricidia+ avocado with the highest (L+P)/N of 64. As control soil without litter application was used. Applying tree litters regardless their quality increased significantly (p<0.05) all growth parameters of earthworm, except for Gliricidia application lead to higher level of earthworm mortality starting at 20 days after treatment. Applied a low quality of avocado litter to the soil produced the highest biomass (0.79 g/indiv.), diameter (2.42 mm/indiv.) and length (6.13 cm/indiv.). Mixing coffee litter with Gliricidia litter increased earthworm mortality (5.5% to 42.5%) and reduced production of cocoon (2 become 0 cocoons) compared to coffee applied alone. Coffee+Gliricidia+Avocado lead to longer live earthworm up to 80 days Apparently Gliricidia litter producing chemical substances which harmful to earthworm.Keyword: earthworm, litter quality, forest conversion
Transfer knowledge of organic agriculture for healthy horticulture cultivation on the Bengawan Solo River, Central Java Widyatmani Sih Dewi; Supriyadi Supriyadi; Mujiyo Mujiyo; Desti Dian Amalina; Muhammad Rizky Romadhon
Community Empowerment Vol 8 No 3 (2023)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.8104

Abstract

Organic farming practices are needed to maintain soil fertility and the health of horticultural products on the banks of the Bengawan Solo river in Jangglengan village, Sukoharjo. This community service aims to teach the farming community about organic farming principles and the practice of making liquid organic fertilizer to support healthy horticultural farming on riverbanks in the Jangglengan area. The partner group, the Jangglengan village government, involves 60 farmer representatives. The methods used were focus group discussion, practices for making liquid organic fertilizer, and evaluation. The results of community service activities show that 75% farmers can show good organic waste material for organic fertilizer, and 95% farmers had improved skills and were able to try making fertilizer by themselves. This organic farming socialization is a good start toward implementing good agricultural practices on the land along the Bengawan Solo River in Jangglengan.
Characteristics of Indigenous Bacterial Isolates from Cocoa Plantations in Meko Village, Central Sulawesi, with Ability to Degrade Cellulose Anak Agung Sagung Putri Risa Andriani; Widyatmani Sih Dewi; Novizar Nazir; Ni Luh Putu Putri Setianingsih; Eko Rini Indrayatie; Krisnawan Kalimutu
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 7 No. 2 (2023)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.312 KB)

Abstract

The characteristics of indigenous bacterial isolates in cocoa farms differ based on the study's location and methodology. More research is required to comprehend these bacterial isolates' diversity and features completely. This study intends to assess the cellulose-degrading capacity of bacterial isolates from cocoa plantations in Meko Village, Central Sulawesi. The shape of the bacterial colony, the shape of the colony's edge, elevation, colony size, color, and texture are considered during macroscopic morphological identification and microscopic morphological identification after gram stain. Afterward, gram stains the bacteria to determine the type of cell wall. Hydrolysis capacity was then identified macroscopically by observing colony shape, colony periphery, colony color, colony appearance and height, and hydrolysis capacity on media in a petri dish (HC). Upon microscopic examination of cell shape and bacterial wall type (Gram-positive and gram-negative). Based on the research findings and identification of bacteria capable of degrading cellulose in cocoa soil, it can be inferred that among the 28 examined isolates, six isolates have the greatest HC index values in KL62 isolates. In contrast, GL66, KL23, and KL62x isolates have medium HC index values.
Land suitability evaluation for Curcuma zedoaria Rosc. in the community forest of the upstream Bengawan Solo watershed, Wonogiri, Indonesia Widyatmani Sih Dewi; Supriyadi Supriyadi; Purwanto Purwanto; Aisyah Defara Rahmadani; Wahyu Galang Pranata; Ganjar Herdiansyah
Journal of Degraded and Mining Lands Management Vol 10, No 3 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2023.103.4369

Abstract

The soil in the upstream Bengawan Solo watershed is at high risk of degradation related to a high erosion rate. Finding the proper crop type with land suitability is critical to protect against further soil degradation. This study aimed to determine the land suitability of white turmeric (Curcuma zedoaria) and the limiting factors in the upstream Bengawan Solo watershed community forest. This research was descriptive-explorative based on a survey of four land map units (LMUs) of Sumberejo village (LMU1, LMU2, LMU3, and LMU4), Batuwarno, Wonogiri. The number of sample points at each LMU depended on the total area of the LMU and was repeated four times. LMU results from overlay soil type maps, administrative maps, rainfall maps, and slope maps. Land suitability assessment was analyzed using the matching method. The land suitability classes of the upstream Bengawan Solo for white turmeric were S3 eh, and N eh. The limiting factors on land suitability for white turmeric are erosion hazards and slopes. The efforts to improve the limiting factor can be made by contour cultivation and repairing the terrace.
Determinan Terpilih untuk Kualitas Air Embung pada Lahan Tadah Hujan di Karanganyar, Jawa Tengah Achmad Adi Surya Sustama; Komariah Komariah; Arwa Farida Lukito; Sumani Sumani; Dwi Priyo Ariyanto; Widyatmani Sih Dewi
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 3 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.3.497

Abstract

Water conservation is needed to overcome the shortage of irrigation water through harvesting rainwater on rainfed land using small farm reservoir. Intensive agricultural activities cause a reduction in land resources which disrupts hydrological functions (water quantity and quality). This study aims to determine the selected parameters for small farm reservoir water quality and identify water quality based on irrigation water standards according to FAO (1994) and PP No. 82 of 2001. The research was conducted from March to December 2014 in Karanganyar Regency, Central Java Province. This study used a descriptive exploratory survey method with purposive sampling. Water quality data was analyzed using Principal Components Analysis (Multivariate Test) MINITAB 16 to determine the main components as the selected determinants. Furthermore, based on the results of the PCA analysis, 5 variables were obtained whose eigenvalue proportions met the main criteria, including DHL of 0.467; SAR of 0.542; pH of 0.538; Actual Savings Ratio (ASR) of 0.662; and water-air temperature deviation of 0.612. Furthermore, a match is made between the selected determinant measurement data and the water quality standard. The results of matching to the selected determinants for water quality in the reservoir have met the water quality standards for irrigation based on FAO (1994) and Government Regulation no. 82 (2001). The soil characteristics of the catchment area also affect water quality because in rainwater harvesting, surface runoff in the catchment area will flow and be accommodated by the small farm reservoir. Keywords: water quality, small farm reservoir, PCA, water conservation, rainfall harvesting
Consistency of Mycorrhizal Effectiveness on Maize Growth and P Uptake in Two Generations of Pot Culture Using Andisol-Based Media Vita Ratri Cahyani; Suryanti Suryanti; Slamet Minardi; Widyatmani Sih Dewi
AGRIVITA, Journal of Agricultural Science Vol 45, No 2 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i2.3865

Abstract

The functional roles of Arbuscular Mycorrhiza (AM) in soils with P limitations are well documented. However, the protocol to produce effective AM inocula was still limited. This research aims at obtaining the effective AM culture in handling P constraints for maize growth on Andisols. The first experiment of pot culture I was to propagate and examine the effectiveness of AM isolates by using a factorial completely randomized design with two factors, A=media (A0: zeolite; A1: representative media=Andisol Tengaran; A2: typical media=a mixture of Andisol Tengaran+Tawangmangu; A3: typical media+Bio-RP nutrition; A4: Inceptisol) and I = AM inoculum source (I0: no inoculum; I1: AM from Andisol Tengaran; I2: AM from Andisol Tengaran + Tawangmangu; I3: AM from 8 soil types), with six replications. The second experiment investigated the consistency of mycorrhizal effectiveness by reculturing AM cultures generation I to pot cultures generation II with the same composition of the respective media. The combination treatments of A1I3, A2I2, A0I3, and A4I3 (AM cultures generation I), continued by A1I32, A2I22, A0I32, and A4I32 (AM cultures generation II) showed consistently the highest AM infectivity and effectiveness on maize growth and P uptake on Andisol-based media, and on the comparison media of zeolite and Inceptisol media, respectively.
Co-Authors Achmad Adi Surya Sustama Ahmad Yunus Aisyah Defara Rahmadani AKTAVIA HERAWATI Anak Agung Sagung Putri Risa Andriani Anak Agung Sagung Putri Risa Andriani Anggita, Akas Arlindo Fernando Macie Arwa Farida Lukito Atmanto Heru Wibowo Atmanto, Heru Ayu Dian Fataya Azhar Fauzan, Adhia Bambang Pujiasmanto Canggih Jati Nusantara Canggih Jati Nusantara, Canggih Jati Desti Dian Amalina Dwi Priyo Ariyanto Efendi, Syahrul Eko Rini Indrayatie Farida Yuliani Faza Nur Afiah Ganjar Herdiansyah, Ganjar Hardian, Tiara Hasanah, Khalyfah Heca Rahma Safira Aulika Nurhadi Herwin Setyaningsih Hery Widijanto Hiramatsu, Ken Ika Ernawati Indratin Indratin Indratin Indratin Intan Nur Fitriyani Irmawati, Viviana Istiqomah, Nanda Mei Jauhari Syamsiyah Kalimutu, Krisnawan Kesya Valencia Putri Susanto Komariah K Komariah Komariah Komariah Komariah Krisnawan Kalimutu Kurniatun Hairiah Loveana, Okta MMA Retno Rosariastuti Muhammad Rizky Romadhon Mujiyo Mujiyo Ni Luh Putu Putri Setianingsih Ni Luh Putu Putri Setianingsih Noorcahyati Noorcahyati Novizar Nazir Nur Sabilla, Widya Putri Nuraini Dwi Agustina Putri Ongko Cahyono Prabang Setyono Pungky Slamet Wisnu Kusuma Purnomo Edi Sasongko Purwanto Purwanto Purwanto Purwanto Purwanto Purwanto PUTRI HANDAYANI Putri Permatasari Rachmawatie, Srie Juli Rahayu Rahayu Rahayu Rahayu Rahayu Rahayu Ramdan Hidayat Rani Rahmawati Retno Rosariastuti Retno Rosariastuti Romadhon, Muhammad Rizky Sapja Anantanyu Slamet Minardi Slamet Minardi Sri Hartati Sri Rossati Sri Rossati Sri Wahyuni Sri Wahyuni Sudadi Sudadi Sudadi Sudadi Sudadi Sudadi Sudjono Utomo Sulandjari Sulandjari Sumani Sumani Sumani Sumani Sumarno . Sumarno Sumarno Sumarno Sumarno Sumarno Sumarno Suntoro Suntoro Suntoro Suntoro Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Supriyadi Suryanti Suryanti Suryono Suryono Suryono Suryono Sutami Sutami Sutarno Sutarno Timur Priono Usman Siswanto Vita Ratri Cahyani Vita Ratri Cahyani Vita Ratri Cahyani Wahyu Adi Nugroho Wahyu Galang Pranata Yoviana Erdhika Adiarti