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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Biomedicine and Translational Research Indonesian Journal of Biotechnology Majalah Obat Tradisional The Indonesian Biomedical Journal Molecular and Cellular Biomedical Sciences (MCBS) ACI (Acta Cardiologia Indonesiana) Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) International Journal of Applied Sciences and Smart Technologies JURNAL PENELITIAN KESMASY Indonesian Archives of Biomedical Research (InABR)
Nur Arfian
Departement Of Anatomy, Embryology, And Anthropology, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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ZEB1 is Negatively Correlated with E-Cadherin in Prostatic Anomaly Tissue Sari Eka Pratiwi; Sri Nuryani Wahyuningrum; Rachmagreta Perdana Putri; Danarto Danarto; Didik Setyo Heriyanto; Nur Arfian; Sofia Mubarika Haryana; Indwiani Astuti
Molecular and Cellular Biomedical Sciences Vol 6, No 1 (2022)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v6i1.220

Abstract

Background: Prostatic anomalies are common in tumor or infection condition. The enlargement of prostate gland affects the epithelial cell polarity that involves epithelial-mesenchymal transition (EMT). Transition into mesenchymal is mediated by transcription factor ZEB1 and E-cadherin protein. Upregulation of ZEB1 and loss of E-Cadherin expression were associated to proliferation and metastasis of malignancy cells. This study aims to describe the correlation of ZEB1 and E-cadherin expression in prostatic anomaly.Materials and method: Samples were Formalin Fixed Paraffin Embedded (FFPE) block consist of 8 block Benign Prostatic Hyperplasia (BPH), 6 blocks High Grade Prostatic Intraepithelial Neoplasia (HGPIN) and 6 blocks Prostate Carcinoma (PCA). The blocks then sliced into 5 sections to be prepared for RNA extraction procedures. ZEB1 and E-Cadherin expression was analyzed by semi-quantitative procedures using PCR and electrophoresis. Correlation between ZEB1 and E-Cadherin espression was analyzed using Spearman’s rank correlation.Results: Relative expression of ZEB1 and E-cadherin mRNA in each group of prostatic anomaly were not significantly different (p>0.05). ZEB1 and E-Cadherin mRNA expression showed a significant and moderate level of negative correlation (p<0.05; 0.40 < r < 0.59). Increasing of ZEB1 mRNA expression will be followed by decreasing of E-Cadherin mRNA expression.Conclusion: ZEB1 negatively correlates with E-cadherin due to EMT process in prostatic anomaly. High expression of ZEB1 induced down-regulation of E-cadherin and vise versa. Various studies can be developed, especially the development of targeted therapy against ZEB1 to suppress the EMT process by increasing the expression of E-cadherin.Keywords: epithelial-mesenchymal transition (EMT), ZEB1, E-Cadherin, BPH, HGPIN, PCA
IS CADAVER STILL NEEDED IN MEDICAL EDUCATION? Muhammad Mansyur Romi; Nur Arfian; Dwi Cahyani Ratna Sari
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 8, No 3 (2019): November
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.233 KB) | DOI: 10.22146/jpki.46690

Abstract

Background: Medical education has been developing very rapidly. Cadavers, which can serve as very important learning resources, need special concern for their availability and utility.Methods: This narrative review is based on related articles freely downloaded which discuss the role and availability of cadavers worldwide. The recent Indonesian condition with Universitas Gadjah Mada as a case will be described.Results: Cadavers play important roles in several aspects, from bioethics and behavioral development until clinical skills, in basic education and especially in postgraduate and advance clinical training. The availability of cadavers has declined significantly, and as a result, the impacts must be considered. Most cadavers are unidentified bodies, eventhough presently a body donation program is recommended as the alternative source.Conclusion: Currently in Indonesia, timely participation is needed from various stakeholders to support effective and efficient cadaver availability and utility. Additional learning resources are needed to be developed in order to assure quality improvement of medical education.Keywords: cadaver, medical education, bioethics, unidentified bodies, body donation.
PENGARUH LATIHAN KESEIMBANGAN METODE VLADIMIR JANDA TERHADAP RISIKO CEDERA OLAHRAGA BASKET PADA PELAJAR LAKI-LAKI SMP NEGERI 15 MEDAN Nondang Purnama Siregar; Santosa Santosa; Nur Arfian
Jurnal Penelitian Kesmasy Vol 1 No 2 (2019): Jurnal Penelitian Kesmasy
Publisher : Fakultas Kesehatan Masyarakat Institut Kesehatan Deli Husada Delitua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36656/jpksy.v1i2.151

Abstract

The incidence of injuries in the sport of basketball was ranked third after football and handball. Basketball is the learning materials required in junior high school through the physical education lesson. Balance training program as an effort to prevent the occurrence of the injury shown to reduce postural sway, increase muscle strength and improve balance strategy. The objectives of the research was to assess the effect of Vladimir Janda balance training method against the risk of injury to the male students at SMP Negeri 15 Medan, Indonesia. The research used quasi-experimental study with nonrandomized control group pretest posttest design. The subjects were male students aged 12-15 years who follow basketball extracurricular. Each group consisted 11 male students. Subject exercise basketball 2 times a week for 50 minutes. Vladimir Janda balance training method performed for 5 weeks and consists of 5 phases. Phase I- IV carried out 5 times a week, while Phase V is done 3 times a week and each session lasted 10 minutes. Postural sway measured by posturometer. Body part discomfort was measured by Nordic Body Map (NBM) questionnaires. The statistical test used was the Independent t-test. There is a decrease in the frequency of postural sway of the right foot(61%) between exercise and control group, musculoskeletal pain has decreased but not statistically significant.
Correlation between Nephrin expression, tubular injury, and serum Creatinine level in kidney failure model with 5/6 subtotal Nephrectomy in mice M. Mansyur Romi; Dwi Cahyani Ratna Sari; Riky Setyawan; Fauziyatul Munawaroh; Nur Arfian
Indonesian Archives and Biomedical Sciences Vol 1 No 1 (2021): Indonesian Archives and Biomedical Sciences Vol 1(1): 2021
Publisher : Konsorsium Ilmu Biomedik Indonesia (KIBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1045.16 KB)

Abstract

Background: Chronic kidney diseases (CKD) is characterized by glomerulosclerosis, tubular injury, and proteinuria. Neprin is the one of the most important protein involved in glomerular filtration but the mechanism of nephrin expression in chronic kidney failure is not well understood. Objective: We aims to elucidate the correlation between nephrin expression with tubular injury and serum creatinine level. Methods: We performed 5/6 subtotal nephrectomy (SN) in male strain Swiss mice to induce CKD. Sham operation was performed to control group (SO) (n=8). Mice were sacrificed in day 7 (SN7; n=8) and day 28 (SN28; n=8) after operation. We measure creatinine serum level to assess renal function. Tubular injury score was quantified using Periodic Acid Schiff (PAS) staining. Reverse transcriptase PCR (RT-PCR) was carried out to examine Nephrin mRNA expression. Results: 5/6 subtotal nephrectomy induced an increased of serum creatinine level in SN7 and SN28 (p<0,01 vs SO), followed by an increased of tubular injury score in SN7 and SN28 (p<0,01 vs SO). We confirmed reduction of nephrin expression in SN28 (p<0.01 vs SO). There was a negative correlation between nephrin and tubular injury (r=0.719, p<0.01) and the positive correlation between tubular injury and serum creatinine level (r=0.891, p<0.01). However, we did not find any significant correlation between nephrin expression and serum creatinine level. Conclusion: Nephrin expression downregulation might represent renal function disturbtion in CKD.
The Effects of Exercise on Spleen Fibrosis and Macrophage Number in D-Galactose-Induced Aging Rat Model Pramita Dwinda Mulyana; Widya Wasityastuti; Fajar Dwi Astarini; Nungki Anggorowati; Nur Arfian
Journal of Biomedicine and Translational Research Vol 8, No 2 (2022): August 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v8i2.14199

Abstract

Background: Spleen plays a role in human immune system as well as in recycling and filtering blood. The aging spleen is associated with fibrosis and impaired immune system, increasing individual vulnerability to getting infections. Regular physical activity is essential to maintain and enhance body fitness, endurance, and immune system.Objective: This study aimed to investigate the effects of mild and moderate-intensity treadmill exercise on the aging spleen by examining fibrosis and the number of macrophages in d-Galactose-induced aging rat models.Methods: Twenty-four male Wistar rats were randomly divided into four groups: G1 (control group), G2 (d‐Galactose, no exercise), G3 (d‐Galactose, low-intensity exercise), and G4 (d‐Galactose, moderate-intensity exercise). d-Galactose was administered intraperitoneally on day 0 and treadmill exercise was given for four weeks following the modified Brown et al. (2007) protocol. Spleens were histologically processed and stained for picrosirius red and against CD68+ antibody. Percentage of fibrosis fraction area and macrophage cell count were obtained using ImageJ, and the data were analyzed statistically using SPSS software.Results: The aging spleen did not show any differences in weight, length, and width among groups (P > 0.05). The administration of d-Galactose in rats causes fibrosis and an increased number of macrophages. Low and moderate-intensity treadmill exercise could not lower the percentages of fibrosis fraction area. However, the moderate-intensity treadmill exercise was effective in lowering macrophage number.Conclusion: Moderate-intensity treadmill exercise was effective in lowering macrophage cell count in the aging rat spleen induced by d-Galactose.
Interactive Learning Media Anatomy of 3D Human Skull Using Augmented Reality Maharsa Pradityatama; I G.B. Budi Dharma; Nur Arfian
Jurnal JTIK (Jurnal Teknologi Informasi dan Komunikasi) Vol 7 No 2 (2023): APRIL-JUNE 2023
Publisher : KITA Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35870/jtik.v7i2.787

Abstract

Anatomy is the study of the structure of the body and the relationship of its parts to one another. Studying human anatomy is mandatory for students of the Faculty of Medicine, especially Medical Education. Based on the results of a preliminary study conducted by the author on students of the Faculty of Medicine, some students stated that they did not understand studying human anatomy only by reading anatomy books such as Sobotta's Atlas of Human Anatomy, Yokochi's Color Atlas of Anatomy, practicum modules. This gave rise to the idea to design an android-based smartphone application using Augmented Reality technology for interactive learning media. Making AR smartphone applications using Unity and Vuforia software with C# programming language. The result of this research is to develop an interactive learning media application of a 3D human skull using augmented reality technology. Interactive learning media provide convenience for students in studying the anatomy of the human skull
Smoothing Module for Optimization Cranium Segmentation Using 3D Slicer Gilang Argya Dyaksa; Nur Arfian; Herianto Herianto; Lina Choridah; Yosef Agung Cahyanta
International Journal of Applied Sciences and Smart Technologies Volume 05, Issue 01, June 2023
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/ijasst.v5i1.6300

Abstract

Anatomy is the most essential course in health and medical education to study parts of human body and also the function of it.  Cadaver is a media used by medical student to study anatomical subject. Because of limited access to cadaver and also due to high prices, this situation makes it necessary to develope an alternative anatomical education media, one of them is the use 3D printing to produce anatomical models. Before 3D Print the cranium, it is necessary to do the segmentation process and often the segmentation result is not good enough and appear a lot of noises. The purpose of this research is  to optimize a 3D cranium based on DICOM (digital imaging and communications in medicine) data processing using the smoothing modules on 3D Slicer. The method of this research is to process the Cranium DICOM data using 3D Slicer software by varying the 5 types of smoothing modules. The results with default parameter fill holes and median have better results compared to others. Kernel size variations are performed for smoothing module fill holes and medians. The result is fill holes get optimal segmentation results using a kernel size of 3 mm and the median is 5 mm
PENGARUH PEMBERIAN ASAM KLOROGENAT TERHADAP EKSPRESI mRNA TLR-4, mRNA MCP-1, JUMLAH MAKROFAG M1 DAN M2 PADA GINJAL MENCIT SWISS WEBSTER DENGAN MODEL UNILATERAL URETERAL OBSTRUCTION (UUO) Tiara Meirani Valeria Savista; Junaedy Yunus; Nur Arfian
Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Vol 5 No 2 (2023): Agustus
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v5i2.130

Abstract

Penyakit ginjal kronis (CKD) adalah masalah kesehatan global dengan prognosis buruk yang mengarah ke fibrosis ginjal. Obstruksi ureter unilateral (UUO) adalah metode yang representatif untuk memodelkan fibrosis ginjal. Penelitian ini merupakan penelitian kuasi eksperimen dengan desain kelompok post-test dengan 25 ekor mencit Swiss Webster jantan dewasa yang dibagi menjadi lima kelompok, yaitu SO, U7, U14, UC7, UC14. Ekspresi mRNA TLR-4 diperiksa dan mRNA MCP-1 diperiksa oleh RT-PCR. Jumlah makrofag M1 dan M2 dengan pewarnaan IHC. Data diproses menggunakan SPSS. Pada kelompok yang diinduksi UUO dan yang diinjeksi Aquadest, ekspresi mRNA TLR-4, mRNA MCP-1 dan jumlah makrofag M1 dan M2 lebih tinggi daripada kelompok kontrol (p<0,005), sedangkan kelompok yang diinduksi UUO dan diinduksi kelompok asam klorogenik menunjukkan mRNA TLR-4, mRNA MCP-1, jumlah makrofag M1 yang lebih rendah dan jumlah makrofag M2 yang lebih tinggi dibandingkan dengan kelompok UUO yang diinjeksi dengan Aquadest (p<0,005). Pemberian asam klorogenat dapat mencegah fibrosis ginjal dengan cara menurunkan ekspresi mRNA TLR-4, mRNA MCP-1, jumlah makrofag M1 dan meningkatkan jumlah mRNA M2.
PENGARUH PEMBERIAN ASAM KLOROGENAT PADA MENCIT SWISS WEBSTER DENGAN MODEL UNILATERAL URETERAL OBSTRUCTION Masita Muchtar; Nur Arfian; Junaedy Yunus
Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Vol 5 No 2 (2023): Agustus
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v5i2.131

Abstract

Penyakit ginjal kronis terlihat dari kerusakan tubular, peradangan dan fibrosis interstisial. Anemia merupakan komplikasi dari gagal ginjal kronis. Transforming growth factor β1 (TGF-β1) dan Snail memainkan peran penting dalam fibrosis ginjal. Asam klorogenik memiliki efek renoprotektif dan antifibrotik, namun efek asam klorogenat terhadap kadar hemoglobin, fraksi area fibrotik, ekspresi mRNA TGF-β1 dan mRNA koklea masih belum diketahui. Penelitian ini merupakan penelitian eksperimen dengan desain post-test only dan 25 ekor mencit Swiss Webster jantan dewasa yang dibagi menjadi lima kelompok yaitu SO, U7, U14, UC7 dan UC14. Tingkat hemoglobin diperiksa dengan hematology analyzer, proporsi area fibrotik diwarnai dengan Sirius Red. mRNA TGF-β1 dan mRNA siput dianalisis dengan RT-PCR. Data dikuantifikasi dengan ImageJ dan diproses dengan SPSS. Kelompok yang diinduksi UUO tanpa pemberian asam klorogenat menunjukkan hemoglobin lebih rendah, area fibrotik lebih rendah, ekspresi TGF-β1 dan mRNA etana lebih tinggi dibandingkan kelompok dengan nilai p <0,005, kelompok yang diinduksi UUO dan diberi asam klorogenik mencapai kadar hemoglobin lebih tinggi, fraksi area serat lebih tinggi, ekspresi TGF-β1 dan mRNA koklea lebih rendah daripada kelompok U14, p<0,005. Pemberian asam klorogenik menunjukkan kadar hemoglobin lebih tinggi, proporsi area fibrotik, dan ekspresi mRNA TGF-β1 dan mRNA siput lebih rendah daripada kelompok yang tidak menerima asam klorogenat.
Achatina fulica Mucus Ameliorates UVB-induced Human Dermal Fibroblast Photoaging via the TGF-β/Smad Pathway Christiana Tri Nuryana; Tiara Puspita Agustin; Sofia Mubarika Haryana; Yohanes Widodo Wirohadidjojo; Nur Arfian
The Indonesian Biomedical Journal Vol 15, No 6 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i6.2580

Abstract

BACKGROUND: Ultraviolet B (UVB) induces skin photoaging by reducing collagen deposition via impairment of the TGF-β/Smad signaling pathway. Achatina fulica mucus (AFM) is a native medicine acting as vehicle of anti-aging ingredients. The present investigation examined the effect of AFM on UVB-induced fibroblast photoaging by assessing TGF-β, Smad3, and Smad7 mRNA expressions.METHODS: AFM was extracted from A. fulica using electrical shock and freeze-dried into a powder. Normal human dermal fibroblast (NHDF) cultures were irradiated with/without 100 mJ/cm2 UVB and treated with/without 10% platelet-rich plasma or different concentrations of AFM: 3.9 μg/mL in AF3 group; 15.625 μg/mL in AF15 group, and 62.5 μg/mL in AF62 group. The mRNA expressions of TGF-β, Smad3, and Smad7 in NHDF were evaluated by quantitative polymerase chain reaction.RESULTS: TGF-β mRNA expressions in the AF3 (0.85±0.01), AF15 (0.94±0.02) and AF62 (1.64±0.03) groups were significantly higher (p<0.05) compared with that in the UVB group (0.55±0.04). Moreover, Smad3 expressions in the AF3 (1.42±0.25), AF15 (1.89±0.13), and AF62 (2.50±0.31) groups were significantly higher (p<0.05) compared with that in the UVB group (0.57±0.08). Furthermore, Smad7 expressions in the AF3 (1.57±0.18), AF15 (0.87±0.03), and AF62 (0.25±0.09) groups were significantly lower (p<0.05) than that in the UVB group (2.57±0.06).CONCLUSION: AFM ameliorates UVB-induced fibroblast photoaging by upregulating the TGF-β/Smad3 expressions and downregulating Smad7 expression.KEYWORDS: Achatina fulica, TGF-β, Smad, collagen, UVB, fibroblast, photoaging
Co-Authors Afifah Afifah Amelia Amelia Arif Prassetyo Baiq R Rhadianaa Christiana Tri Nuryana Danarto Danarto Danarto Danarto Daniel Agriva Tamba Dian Prasetyo Wibisono Didik Setyo Heriyanto Dwi Aris Agung Nugrahaningsih Dwi Cahyani Ratna Sari Dwina Agrila Lakabela Eko Purnomo Fajar Dwi Astarini Fauziyatul Munawaroh Ferdinandus Bayu Satria Gharini, Putrika Prastuti Ratna Gilang Argya Dyaksa Hana Rutyana Putri Antonio Handoyo Pramusinto Herianto . Herianto Herianto Heru Yulianto Hilma Kholida Ats-tsani I Dewa Putu Pramantara I G.B. Budi Dharma Indwiani Astuti Indwiani Astuti Irwan Supriyanto Juananda, Desby Junaedy Yunus Lina Choridah M. Mansyur Romi Mae Sri Hartati Wahyuningsih Maharsa Pradityatama Masita Muchtar Mawaddah Ar-Rochmah Muhammad Mansyur Romi Ngatidjan Ngatidjan Nondang Purnama Siregar Nungki Anggorowati Nunu N Madjid Nur Amin Nyoman Kertia Pramita Dwinda Mulyana Pratiwi Indah Sayekti Rachma Greta Perdana Putri Riky Setyawan Ronny Martien Ronny T Wirasto Santosa Santosa Sari Eka Pratiwi Sofia Mubarika Haryana Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Susini R Sari Tety Hartatik Tiara Meirani Valeria Savista Tiara Puspita Agustin Toni Febriyanto Widya Wasityastuti Widya Wasityastuti Wiwit Ananda Wahyu Setyaningsih Yeshua Putra Krisnugraha Yohanes Widodo Wirohadidjojo Yosef Agung Cahyanta