Article Per Year (5 Year)
p-Index From 2021 - 2026
0.92
P-Index
This Author published in this journals
All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Global Medical and Health Communication Indonesian Journal of Cancer Journal of Medicine and Health Indonesian Journal of Obstetrics & Gynecology Science Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia)
Tono Djuwantono
Departement Of Obstetrics And Gynecology, Faculty Of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung
Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

Published : 20 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 20 Documents
Search

 1   2 
Differences in Expulsion on Post-placenta Intrauterine Contraceptive Device between Mother with Vaginal and Cesarean Delivery Atika Zahria Arisanti; Tono Djuwantono; Sri Endah Rahayuningsih
Global Medical & Health Communication (GMHC) Vol 8, No 1 (2020)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.221 KB) | DOI: 10.29313/gmhc.v8i1.3688

Abstract

Intrauterine device (IUD) is a long term, highly effective, and reversible contraception method. In Indonesia, the number of IUD acceptors is still lower than other methods. An effort to improve the long term contraception is using post-placental IUD that can be a choice for postpartum mother who has limited access to another contraception service. The purpose of this study was to compare the incidence of expulsion in post-placental IUD insertion between mother with vaginal delivery and cesarean delivery. This study design was a comparative cross-sectional method with a consecutive sampling technique conducted at Dr. Hasan Sadikin General Hospital Bandung and Dr. Kariadi General Hospital Semarang from November 2017 to February 2018. Subjects were postpartum mothers who received post-placental IUD insertion at vaginal delivery and cesarean delivery. Samples were 96 women, consisting of 48 women with IUD insertion in vaginal delivery and 48 women with IUD insertion in cesarean delivery. Data obtained from interviews and transvaginal ultrasonography examination. The result showed there was a difference in expulsions incidence between IUD’s insertion among vaginal delivery compared to cesarean delivery (p=0.041). It concluded that expulsion’s to post-placental IUD insertion is higher in vaginal delivery compared to cesarean delivery. PERBEDAAN KEJADIAN EKSPULSI PADA PEMASANGAN ALAT KONTRASEPSI DALAM RAHIM PASCAPLASENTA ANTARA IBU DENGAN PERSALINAN PER VAGINAM DAN PERSALINAN SECTIO CAESAREAAlat kontrasepsi dalam rahim (AKDR) merupakan kontrasepsi jangka panjang, efektif dan reversibel. Di Indonesia, jumlah akseptor AKDR masih lebih rendah daripada metode lainnya. Salah satu upaya untuk meningkatkan penggunaan kontrasepsi jangka panjang, yaitu dengan AKDR pascaplasenta yang dapat menjadi alternatif bagi ibu pascasalin yang mempunyai akses terbatas untuk mendapatkan pelayanan kontrasepsi. Penelitian ini bertujuan membandingkan kejadian ekspulsi pada pemasangan AKDR pascaplasenta antara ibu dengan persalinan per vaginam dan persalinan sectio caesarea. Desain penelitian ini adalah cross-sectional komparatif dengan teknik pengambilan sampel consecutive yang dilaksanakan di RSUP Dr. Hasan Sadikin Bandung dan RSUP Dr. Kariadi Semarang dari bulan November 2017 hingga Februari 2018. Subjek penelitian adalah ibu pascasalin yang mendapatkan insersi AKDR pascaplasenta pada persalinan per vaginam dan persalinan sesar. Jumlah sampel 96 ibu, terdiri atas 48 ibu yang bersalin per vaginam dan 48 ibu yang bersalin sesar. Data didapatkan melalui wawancara dan pemeriksaan ultrasonografi transvaginal. Hasil penelitian menunjukkan terdapat perbedaan kejadian ekspulsi pada pemasangan AKDR antara ibu dengan persalinan per vaginam dan persalinan sectio caesarea (p=0,041). Simpulan, kejadian ekspulsi pada pemasangan AKDR pascaplasenta lebih tinggi pada ibu dengan persalinan per vaginam dibanding dengan persalinan sectio caesarea.
Analisis Faktor yang Memengaruhi Keberhasilan Kehamilan pada Inseminasi Intrauterin Angghea Rachmiawaty; Tono Djuwantono; R. M. Sonny Sasotya
Indonesian Journal of Obstetrics & Gynecology Science Volume 1 Nomor 1 Maret 2018
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1314.612 KB) | DOI: 10.24198/obgynia.v1n1.42

Abstract

AbstrakTujuan: Inseminasi intrauterin (IIU) merupakan prosedur yang umum digunakan dan menjadi pilihan terapi pertama dalam tatalaksana infertilitas, karena dampak risiko yang rendah, implementasi yang mudah, dan harga yang murah. Metode: Penelitian cross-sectional ini dilakukan pada seluruh pasangan yang melakukan prosedur IIU di Poliklinik Aster Rumah Sakit dr. Hasan Sadikin Bandung pada periode 1 Januari 2016 sampai dengan 31 Desember 2016, yang tercatat dalam rekam medik. Variabel penelitian berupa Umur Istri, Umur Suami, Jumlah Sperma, Konsentrasi Sperma, dan Motilitas Sperma, Ketebalan Endometrium, Jumlah Folikel preovulasi, Jenis Stimulasi dan Output. Data dikelola dengan SPSS 24.00. Hasilnya disajikan secara analitik melalui angka dan tabel.Hasil: Diantara 159 pasangan yang melakukan prosedur IIU, terdapat 194 prosedur. Namun hanya 98 subjek yang dapat dievaluasi. Angka kehamilan sebesar 23.5%. Faktor yang mempengaruhi hanya jenis stimulasi ovarium dan jumlah ovum preovulasi yang berhubungan secara signifikan dengan angka kehamilan (p<0,05), umur pasangan, jumlah, konsentrasi, dan motilitas sperma tidak berbeda bermakna.Kesimpulan: Berbagai variabel mempengaruhi keberhasilan dari IIU. Jenis stimulasi ovarium dan jumlah ovum preovulasi berhubungan secara signifikan dengan angka kehamilan (p<0,05). Factors Analizyng of Influencing for Succeses Pregnancy Rate on Intrauterina InseminationAbstractObjective: intrauterine insemination (IUI) is a procedure widely used in fertility management. However, the effectiveness of IUI treatment is not consistent, and the role of multiple factor affecting successes in IUI has not been clarified.Methods: Cross sectional study was conducted on infertile couples performing IUI. Data from medical record at Aster Clinique Dr. Hasan Sadikin General Hospital at January 1st- December 12th, 2016. Spouse Ages, Sperm Count, Concentration, and Motility, Endometrial Thickness, Number of Preovulatory Follicles, Type of Stimulation and Output are research variables. Data was analyzed by SPSS 24.00. Result: Among 159 couples, there were 194 procedures of IUI. Only 98 subject was evaluated. The pregnancy rate was 23.5%. Only type of ovarian stimulation and number of  preovulatory follicle related significantly  to the pregnancy rate (p < 0.05), spouse age, sperm count, concentration and motility, endometrial thickness did not significantly related to the pregnancy rate (p>0,05)Conclusion: There were many variables may influence success rates of IUI. Type of ovarian stimulation and number of  preovulatory follicle related significantly  to the pregnancy rate (p<0.05). More cohort trials and randomized trials investigating the multiple factors affecting successes in IUI are urgently needed.Key words: Intrauterine insemination, infertility, ovarian stimulation
Hubungan Anti-Mullerian Hormone (AMH) pada Long dan Short Protocol Terhadap Fertilization Rate Pasien In Vitro Fertilization (IVF) Rizki Amalia Wahid; Edwin Armawan; Tono Djuwantono
Indonesian Journal of Obstetrics & Gynecology Science Volume 4 Nomor 2 September 2021
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia/v4n2.295

Abstract

Tujuan: Untuk mengevaluasi pengaruh kadar anti-mullerian hormone (AMH) dengan fertilization rate (FR) dan menilai perbedaan pengaruh jenis protokol (long protocol (LP) dan short protocol (SP)) pada tiap tingkat cadangan ovarium terhadap FR pada pasien in vitro fertilization (IVF) dengan Intracytoplasmic Sperm Injection. Metode: Data sekunder dari rekam medis pasien yang menjalani IVF di Aster Fertility Clinic Rumah Sakit Umum Pendidikan dr. Hasan Sadikin pada tahun 2016-2020 dan Bandung Fertility Centre Rumah Sakit Ibu Anak Limijati pada tahun 2018-2019. Penelitian ini analitik observational dengan metode Cohort retrospektif. Hubungan antara dua data kategorik diuji dengan uji chi-square dan uji Kruskal-Wallis digunakan pada data numerik dengan distribusi yang tidak rata pada lebih dari 2 kelompok, Hasil: Hasil data diperoleh nilai rerata kadar AMH secara keseluruhan adalah 3.30 ng/ml dengan rerata capaian FR sebesar 71.97%. Berdasarkan metode IVF yang dipilih, mayoritas pasien menjalani pengobatan SP 54.4% (rerata FR 72.80%) dibandingkan dengan LP 45.6% (rerata FR 70.97%). Tidak ditemukan hubungan yang bermakna antara kadar AMH dengan FR, dinyatakan dengan nilai p=0.977. Kadar AMH terhadap FR bila dipisahkan menurut protokol terapi yang diberikan tidak menunjukkan perbedaan yang bermakna pada masing-masing protokol (LP p=0,763; SP p=0,843). Mengenai hubungan antara protokol IVF dengan FR juga tidak diperoleh perbedaan yang signifikan secara statistik dengan nilai p=0,27 (RR 1.17 (0.62-2.15); CI 95%). Penggobatan menggunakan LP (p=0,770) maupun SP (p=0.845) tidak memberikan pengaruh yang bermakna terhadap FR pada setiap kategori AMH.Kesimpulan: Tidak ada pengaruh kadar AMH dan protokol terapi terhadap FR.Relation of Anti-Mullerian Hormone (AMH) in Short and Long Protocol Againts Fertilization Rate of in Vitro Fertilization (IVF) PatientsAbstractObjective:To evaluate the effect of anti-mullerian hormone (AMH) levels on the success of fertilization rate (FR) and assess the effect of different types of protocols (long protocol (LP) and short protocol (SP)) at each level of ovarian reserve on fertilization rate of in-vitro fertilization (IVF) patients using Intracytoplasmic Sperm Injection.Method: Secondary data from the medical records of patients undergoing IVF at the Aster Fertility Clinic, Hasan Sadikin Hospital in 2016-2020 and Bandung Fertility Center Limijati Hospital. This study was an observational analytic study with a retrospective cohort approach. The relationship between two categorical data was analyzed by chi-square and Kruskal-Wallis on numerical data that were not normally distributed in more than 2 groups.Result: The result of obtained data is an average overall AMH level of 3.30 ng/ml with a mean FR achievement of 71.97%. Based on chosen IVF method, more patient underwent short protocol 54.4% (mean FR 72.80%) than long protocol 46.6% (mean FR 70.97%). There was no significant relationship between AMH levels and FR, expressed by the value of p = 0.977.  AMH levels against FR when separated according to the given therapy protocol did not show any significant difference in each protocol (LP p=0.763, SP p=0.843). Regarding the relationship between the IVF protocol and FR, there was also no statistically significant difference with p value =0.27 (RR 1.17 (0.62-2.15) 95% CI), as well as treatment using LP (p=0,770) or SP (p=0.845) did not have a significant effect on FR at each AMH category (p=0.599).Conclusion: There was no significant relationship between AMH levels and therapy protocol on FR.Key word: In Vitro Fertilization, Fertilization Rate, Anti-Mullerian Hormone, Ovarian Stimulation Protocol
Efusi Pleura Unilateral pada penderita Sindroma Hiperstimulasi Ovarium dalam Program Fertilisasi Invitro (Laporan Kasus) D.S. NATAPRAWIRA; W. PERMADI; T. DJUWANTONO; H. HARLIANTO; H. BAYUAJI
Indonesian Journal of Obstetrics and Gynecology Volume. 31, No. 3, July 2007
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.482 KB)

Abstract

Tujuan: Melaporkan kasus efusi pleura unilateral pada penderita OHSS. Rancangan/rumusan data: Laporan kasus. Tempat: Klinik fertilitas rumah sakit rujukan tersier. Hasil: Seorang wanita usia 32 tahun yang menderita OHSS. Faktor risiko pada kasus ini adalah ovarium polikistik, usia 4300 pg/mL. Saat diketahui respons ovarium yang berlebih, dilakukan penurunan dosis FSH. Terjadi efusi pleura unilateral dan asites minimal 11 hari pasca penyuntikan r-hCG, sehingga perlu dilakukan perawatan intensif dan pemasangan water-sealed drainage (WSD). Dilakukan pemberian albumin intravena yang disesuaikan dengan perkembangan kadar albumin darah harian. Setelah dirawat selama 15 hari pasien dipulangkan dalam keadaan baik. Kesimpulan: Pada kasus ini telah dilakukan monitoring stimulasi secara hormonal dan ultrasonografi serta upaya-upaya pencegahan lain. Namun demikian, OHSS tetap terjadi sehingga diperlukan kombinasi terapi suportif dan bedah. [Maj Obstet Ginekol Indones 2007; 31-3: 174-9] Kata kunci: fertilisasi invitro, sindroma hiperstimulasi ovarium, efusi pleura unilateral
Uji diagnostik modifikasi teknik color Doppler sonohisterosalpingografi bermedia kontras NaCl 0,9% untuk evaluasi patensi tuba T. DJUWANTONO; W. PERMADI; H. BAYUAJI
Indonesian Journal of Obstetrics and Gynecology Volume. 32, No. 3, July 2008
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.545 KB)

Abstract

Tujuan: Mengevaluasi patensi tuba dengan teknik modifikasi color Doppler sonohisterosalpingografi bermedia kontras salin dibandingkan dengan baku emas penilaian patensi tuba, yaitu kromopertubasi perlaparoskopi. Tempat: Penelitian dilakukan di Klinik Teknologi Reproduksi Berbantu Aster, dan Kamar Operasi One Day Surgery RS Dr. Hasan Sadikin Bandung. Bahan dan cara kerja: Pemeriksaan dilakukan terhadap 35 pasien dengan masalah infertilitas, yang tengah menjalani penatalaksanaan infertilitas dasar. Dilakukan penyuntikan cairan salin fisiologis 0,9 % steril ke dalam uterus melalui alat yang telah dimodifikasi, yaitu kateter pediatrik No. 10 yang bermandrin dan spuit 50 cc. Perjalanan cairan diamati dengan USG transvaginal berteknologi Doppler kemudian dilakukan penentuan patensi tuba. Hasil yang didapat dibandingkan dengan hasil kromopertubasi perlaparoskopi dan dianalisis efektivitas diagnostiknya. Dilakukan perhitungan sensitivitas, spesifisitas, nilai prediksi positif, dan nilai prediksi negatif. Dilakukan pula penilaian kavum uteri, yang diharapkan dapat memberikan keuntungan tambahan dari penelitian ini. Hasil: Sensitivitas modifikasi color Doppler sonohisterosalpingografi bermedia kontras NaCl 0,9% adalah 87,5%, spesifisitas 95,5%, nilai prediksi positif 97,7%, dan nilai prediksi negatif 77,8%. Kesimpulan: Teknik modifikasi color Doppler sonohisterosalpingografi bermedia kontras NaCl 0,9% merupakan teknik yang dapat diandalkan untuk evaluasi patensi tuba. [Maj Obstet Ginekol Indones 2008; 32-3: 143-7] Kata kunci: infertilitas, patensi tuba, color Doppler, sonohisterosalpingografi.
Knowledge and attitude of pregnant woman toward umbilical-cord-blood collection Tono Djuwantono
Indonesian Journal of Obstetrics and Gynecology Volume. 34. No. 2, April 2010
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.551 KB)

Abstract

Objective: Stem cell technology from umbilical cord blood (UCB) has developed fast as health services. UCB could be used in many kinds of disease. Private UCB bank has been operated in many places all over Indonesia. There’s a need for research to evaluate the knowledge and attitude of pregnant woman towards the act of collecting UCB. Method: This is a cross-sectional study base on question-naire given to 163 pregnant women in three private hospitals in the Bandung City from September - November 2009. The result was analyzed with Rank-spearman correlation and Kruskal-Wallis chisquare. Result: Most respondent were 20 - 29 years old pregnant women (54.6%), with educational level of strata-1 (S-1) (41.1%), with monthly income within 5 - 10 million rupiah (35.6%). This is the second pregnancy or more (42.9%), with gestational age less than 24 weeks (61.3%) and variative obstetric history (59.5%). The average level of knowledge was poor and attitude scale was uncertain (3.4). There is a significant correlation between age (p = 0.008%; CI 95%), level of education (p = 0.0001; CI 95%) and knowledge. There is a significant correlation between gestational age and attitude (p = 0.003; CI 95%). Respondent’s level of knowledge also has a significant correlation with attitude (τs = 0.421; p = 0.0001; CI 95%). Conclusion: This study described the respondent’s poor knowledge will influence their attitudes toward UCB collection, so it is necessary for disseminating more information on UCB as an effort to get better research result on UCB. [Indones J Obstet Gynecol 2010; 34-2: 97-100] Keywords: knowledge level, attitudes, collecting UCB, pregnant woman, the Bandung city
Dengue Hemorrhagic Fever and its Effect on the Pregnancy Outcomes: A Case Series Farhanah, Aninda Yasmin; Siddiq, Amillia; Djuwantono, Tono; Adriansyah, Putri Nadhira Adinda
Indonesian Journal of Obstetrics & Gynecology Science Volume 7 Nomor 2 Juli 2024
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v7i2.678

Abstract

Background: Dengue fever in pregnancy is associated with a more severe presentation and an increased risk of adverse obstetric and neonatal outcomes. We describe three cases of dengue fever in pregnancy with different fetal outcomes (intrauterine fetal death, fetal distress, and healthy neonate). Case Illustration: The first case involves a 23-year-old G1P0A0 at 31-32 weeks of gestation complaining of reduced fetal movement. She presented with a high-grade fever, anaemic, and thrombocytopenic. Her liver function was increased with AST 447 U/L and ALT 403 U/L. The fetal heart rate could not be detected. The second case involves a 26-year-old G3P2A0 at term pregnancy complaining of labor pain. She presented with vaginal bleeding, high-grade fever, and vomiting five days prior. Her liver function was also increased (AST 301 U/L and ALT 298 U/L). At presentation, fetal distress was detected. The third case involves a 19-year-old G2P1A0 presented with high-grade fever and nausea for five days. Her liver function was moderately increased (AST 68 U/L and ALT 76 U/L). She delivered a 3050-gram healthy neonate vaginally. Discussion: Dengue fever causes adverse obstetric outcomes. Endothelial injury exacerbated by plasma loss leads to placental dysfunction and poor fetal conditions. Unfortunately, dengue fever in pregnancy is not yet specifically addressed in our national guidelines. Conclusion: Increased severity of dengue fever in pregnancy may cause poor maternal and fetal outcomes.Demam Berdarah Dengue dan Dampaknya terhadap Luaran Kehamilan: Sebuah Serial KasusAbstrakPendahuluan: Demam berdarah dengue pada kehamilan terkait dengan tingkat keparahan penyakit dengue yang lebih berat dan meningkatnya risiko komplikasi obstetrik dan neonatus. Serial kasus ini menyajikan tiga kasus demam berdarah pada kehamilan dengan luaran janin yang berbeda (kematian janin intrauterine, gawat janin, dan neonatus sehat). Presentasi Kasus: Kasus pertama, G1P0A0 gravida 31 – 32 minggu, berusia 23 tahun, datang dengan keluhan utama berkurangnya gerakan janin. Keluhan demam, nyeri retro-orbital, mual, dan muntah dirasakan sejak 4 hari sebelumnya. Terdapat tanda konjungtiva anemis, trombositopenia, dan fungsi liver meningkat (AST 447 U/L dan ALT 403 U/L). Detak jantung janin tidak terdeteksi. Kasus kedua, G3P2A0 gravida aterm, 26 tahun, datang dengan keluhan mules-mules, perdarahan jalan lahir, demam, mual, dan muntah dirasakan sejak 5 hari sebelumnya. Pasien mengalami trombositopenia dan peningkatan fungsi liver (AST 301 U/L dan ALT 298 U/L). Pemeriksaan janin menunjukkan tanda gawat janin. Kasus ketiga, G2P1A0 gravida aterm, 19 tahun, datang dengan keluhan utama mules-mules. Keluhan demam dan mual dirasakan sejak 5 hari. Pemeriksaan menunjukkan trombositopenia dan fungsi liver sedikit meningkat (AST 68 U/L dan ALT 76 U/L). Pasien melahirkan neonatus sehat secara spontan dengan berat 3050 gram.Diskusi: Kebocoran plasma merupakan pencetus dari kerusakan endotel sehingga dapat mengakibatkan disfungsi plasenta dan kondisi janin memburuk. Namun, belum ada panduan resmi tatalaksana demam berdarah pada kehamilan di Indonesia.Kesimpulan: Meningkatnya tingkat keparahan demam berdarah pada kehamilan menyebabkan luaran maternal dan neonatus yang buruk.Kata kunci: Demam dengue, maternal, fetus, luaran, kehamilan
Men's Health Infertility Management Djuwantono, Tono
Indonesian Journal of Obstetrics and Gynecology Volume 12 No. 3 Jully 2024
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v12i3.2487

Abstract

N/A
Amniotic Membrane Graft and Hysteroscopic Adhesiolysis as Treatment for Asherman Syndrome Case Rusly, Dewi Karlina; Ritonga, Mulyanusa Amarullah; Rachmawati, Anita; Rinaldi, Andi; Djuwantono, Tono
Indonesian Journal of Obstetrics & Gynecology Science Volume 7 Nomor 3 November 2024
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v7i3.603

Abstract

Introduction: Secondary amenorrhea which caused by intrauterine adhesions is called Asherman’s syndrome. This occurs when the uterine cavity becomes partially or completely blocked, which can damage the basal layer of the endometrium and cause the formation of adhesive cicatricial tissue. The prevalence or incidence of Asherman Syndrome ranges from 2.84-5.5% in women. Case Report: A woman 33 years old had a history of amenorrhea for 2 years and three times curettage due to miscariage. Ultrasound findings showed 1.35 cm long cicatrix in the uterine cavity, and probe had only entered 3 cm. The patient underwent hysteroscopy adhesiolysis and grafting of intrauterine amniotic membrane. The intrauterine catheter was monitored for 1 month. Postoperatively the patient also received estradiol valerate therapy 3 x 2 mg for three months. Management of Asherman syndrome with hysteroscopy adhesiolysis with direct observation accompanied by grafting of amniotic membrane using intrauterine catheter tube is one of the techniques to overcome recurrent intrauterine adhesion. Conclusion:  Secondary amenorrhea in Asherman syndrome is better treated operatively with direct observation of the hysteroscopy and adhesiolysis. The using of amniotic membrane graft and supportive therapy are very helpful for the success of endometrial growth and preventing recurrent adhesions, increase the menstrual volume and chances of pregnancy.Pencangkokan Selaput Ketuban dan Histeroskopi Adhesiolisis sebagai Penatalaksanaan untuk Kasus Sindrom AshermanAbstrakPendahuluan: Amenorea sekunder yang disebabkan oleh perlengketan intrauterin disebut sindrom Asherman dengan prevalensi berkisar antara 2,84 - 5,5%.Laporan Kasus: Seorang wanita berusia 33 tahun mempunyai riwayat amenore 2 tahun dan kuretase sebanyak 3 kali akibat abortus. Temuan USG menunjukkan cicatrix sepanjang 1,35 cm di rongga rahim, sondage hanya masuk 3cm. Pasien menjalani histeroskopi adhesiolisis dan pemasangan cangkok selaput ketuban intrauterin. Kateter intrauterin dipantau selama 1 bulan. Pascaoperasi pasien juga mendapat terapi estradiol valerat 3 x 2mg selama tiga bulan. Penatalaksanaan sindrom Asherman dengan histeroskopi adhesiolisis dengan observasi langsung disertai pemasangan cangkok selaput ketuban menggunakan selang kateter intrauterin merupakan salah satu teknik yang efekstif untuk mengatasi adhesiolisis intrauterin berulang.Kesimpulan: Amenore sekunder pada sindrom Asherman lebih baik ditangani secara operatif dengan observasi langsung berupa histeroskopi dan adhesiolisis. Pemasangan cangkok selaput ketuban dan terapi suportif sangat membantu keberhasilan pertumbuhan endometrium dan mencegah perlengketan berulang, meningkatkan volume darah saat menstruasi dan peluang terjadinya pembuahan.Kata kunci: Adhesi intrauterin, Histeroskopi, Pencangkokan selaput ketuban, Rekonstruksi endometrium, Sindrom Asherman.
Akt–the Mammalian Target of Rapamycin (mTOR) Pathway Inhibition Increases Cervical Cancer Cell Chemosensitivity to Active Form of Irinotecan (SN-38) Septiani, Leri; Hidayat, Yudi Mulyana; Effendi, Yusuf Sulaeman; Djuwantono, Tono; Luftimas, Dimas Erlangga; Faried, Ahmad
International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1048.106 KB)

Abstract

Objective: To investigate the molecular pathway of the cytotoxic effect of SN-38 in human cervical cancer cell lines.Methods: Two human cervical cancer cell lines were treated with various concentrations of irinotecan for 24–72 hours and the sensitivity was analysed using the MTT assay. Apoptosis was further observed through microscopic examinations. The protein expression was determined using Western blot analysis.  Results: CaSki cells demonstrated the highest sensitivity to SN-38, whereas HeLa cells showed the lowest. In cervical cancer cells, SN-38 induced apoptosis through an intrinsic- and extrinsic-pathways. In addition, we showed that SN-38 downregulated the phosphorylation of Akt-mTOR pathways in CaSki cells, but not in HeLa cells. Interestingly, in HeLa cells, which were more suggestive of a resistant phenotype, pre-treatment with LY294002 and rapamycin inhibited activation of Akt-mTOR signaling and significantly enhanced the sensitivity of HeLa cells to SN-38. Conclusions: Irinotecan exerts its anti-neoplastic effects on cervical cancer cells by inducing apoptosis through caspase-cascade. Inhibition of Akt-mTOR, LY294002 and rapamycin, which is targeted to Akt-mTOR pathways, may sensitize irinotecan-resistant cervical cancer cells.Keywords: Akt-mTOR pathways anti-neoplastic drugs, cervix cancer cells, LY294002, rapamycin DOI: 10.15850/ijihs.v1n1.103
Co-Authors Adriansyah, Putri Nadhira Adinda Agung Budi Sutiono Ahmad Faried Amillia Siddiq, Amillia Andi Rinaldi Angghea Rachmiawaty Arief Setiawan Atika Zahria Arisanti D.S. NATAPRAWIRA Danny Halim Dewi Karlina Rusly Dida Akhmad Gurnida Dimas Erlangga Luftimas Edwin Armawan Farhanah, Aninda Yasmin Giyawati Yulilania Okinarum H. BAYUAJI H. BAYUAJI H. HARLIANTO Herry Garna Herry Herman Irvan Afriandi Leri Septiani Muhammad Zafrullah Arifin Nanang W. Astarto, Nanang W. Nurul Auliya Kamila R. M. Sonny Sasotya Rachmawati, Anita Ritonga, Mulyanusa Amarullah Rizki Amalia Wahid Ruswana Anwar Sri Endah Rahayuningsih Susiarno, Hadi Tita Husnitawati Tjandraprawira, Kevin D Tri Hanggono Achmad TRI HANGGONO AHMAD, TRI HANGGONO W. PERMADI W. PERMADI Wiryawan Permadi Yelliantty Yelliantty Yudi Mulyana Hidayat Yusuf Sulaeman Effendi