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All Journal ANNALES BOGORIENSES YARSI Medical Journal Sainstek : Jurnal Sains dan Teknologi Makara Journal of Science Jurnal Kedokteran Hewan Annales Bogorienses
ITA DJUWITA
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

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Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Co-Authors Adi Winarto Arief Boediono Bambang Purwantara Betty Sri Laksmi Jenie Clara M. Kusharto Ekayanti M. Kaiin Ekayanti M. Kaiin, Ekayanti M. Faisal Mustafa Hamny H, Hamny Idawati Nasution Irma Amalia Pratiwi Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati KARTINI ERIANI Katrin Roosita Kusdiantoro Mohamad M Agus Setiadi Muhamad Rizal Martua Damanik Ridawati, Ridawati Rimbawan , Roza Helmita Savitri Novelina SONY HERU SUMARSONO Sri Wahyuni Srihadi Agungpriyono Tuty L. Yusuf TUTY LASWARDI YUSUF Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas WELLYZAR SJAMSURIDZAL Yuhara Sukra