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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JFMR (Journal of Fisheries and Marine Research) Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Grouper: Jurnal Ilmiah Perikanan Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Proceedings Series on Physical & Formal Sciences Journal of Marine Resources and Coastal Management Balobe: Jurnal Pengabdian Masyarakat Journal of Fisheries & Marine Journal of Food and Agricultural Technology
Heru Pramono
Department Of Marine, Faculty Of Fisheries And Marine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Social Sciences Veterinary Other

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Effects of culture conditions on proteolytic activity of Staphylococcus saprophyticus isolated from a traditional fermented condiment Duanty, Putri Valentina; Pramono, Heru; Nirmala, Dwitha
Journal of Marine Resources and Coastal Management Vol. 7 No. 1 (2026)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v7i1.2433

Abstract

Madura petis is a traditional fermented condiment from East Java, Indonesia, made from shrimp or fish cooked with sugar to produce a rich, savory paste. Its characteristic umami flavour develops naturally during storage through spontaneous fermentation involving proteolytic microorganisms. The aims of the study were to analyze the production of protease in different culture condition and nutrition of Staphylococcus saprophyticus (strain PMB1-7) isolated from petis. The isolate was a Gram-positive, catalase-positive coccus that does not ferment mannitol and is resistant to novobiocin, a typical of S. saprophyticus. Growth profiling revealed it reached stationary phase after 12 h of culture. Protease activity assays were conducted under different media, pH levels, and incubation times to determine optimal enzymatic conditions. The highest proteolytic activity occurred in Nutrient Broth (proteolytic index: 1.09 ± 0.01), at neutral pH (1.22 ± 0.10), and after 24 hours of incubation (1.20 ± 0.00). These results offer valuable insights into the enzymatic behavior of S. saprophyticus during petis fermentation and provide a foundation for developing controlled fermentation approaches to enhance the flavour quality of traditional Indonesian condiments.
Molecular Identification and Phylogenetic Reconstruction of Horseshoe Crab (Xiphosura, Limulidae) from the East Java Region Sapto Andriyono; Catlya Tasya Adella Tasya Adella; Mufasirin Mufasirin; Lailatul Lutfiyah; Heru Pramono; Nur Izzati Mohd Noh; Dwi Anggorowati Rahayu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 2 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.2.181-194

Abstract

Horseshoe crabs, ancient marine arthropods of the Limulidae family, are currently facing significant threats due to overexploitation driven by high demand for their eggs, which are widely consumed, and their hemolymph, which is critical for biomedical applications. This study aims to evaluate the molecular identification and phylogenetic placement of horseshoe crabs in East Java, a region previously unreported in this context. DNA barcoding was employed to analyze the mitochondrial COI gene region, complemented by detailed morphological identification, to provide a comprehensive understanding of the species in this area. A universal primer LCO/HCO was used for PCR, as described in a previous report, and Sanger sequencing was performed to confirm the approximately 655 bp amplification product. This research identifies horseshoe crabs from six distinct locations in East Java as Tachypleus gigas using molecular methods, with genetic analysis indicating>99% similarity to T. gigas sequences in the GenBank database. Morphological examinations of specimens from seven areas further corroborated the presence of T. gigas, distinguished by characteristic serrations along the telson and a single spine on the posterior region of the opisthosoma. The phylogenetic tree, constructed from our genetic data, shows that Tachypleus gigas populations in East Java are clustered in the same clade as those in Southeast Asia, as supported by GenBank records. This study underscores the importance of integrating genetic and morphological approaches for accurate species identification. Moreover, it provides crucial baseline data for developing conservation strategies to preserve Tachypleus gigas populations in East Java, thereby contributing to broader conservation efforts for this species.
Valorization of Tuna (Thunnus sp.) Viscera by-Product Into Hydrolysates and Optimizing Bromelain-Mediated Hydrolysis for Functional Applications Rohmah, Arina Maulidatur; Janah, Diana Roudhotul; Pramono, Heru; Pujiastuti, Dwi Yuli
JFMR-Journal of Fisheries and Marine Research Vol. 10 No. 2 (2026): JFMR on July
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2026.010.02.9

Abstract

Tuna (Thunnus sp.) viscera by-product, representing 12–18% (w/w) of total fish biomass, is an underutilized resource that poses environmental challenges if not well managed. However, its high protein content offers significant potential for the production of bioactive protein hydrolysates (FPH). This study aimed to evaluate the impact of bromelain-mediated enzymatic hydrolysis duration (0, 1, 3, and 6 hours) at 54°C on the chemical characteristics and antibacterial efficacy of the resulting hydrolysates against Escherichia coli and Staphylococcus aureus. Results indicated that while the 3-hour hydrolysis treatment yielded the highest protein content (85.109%), the 6-hour treatment provided the highest degree of hydrolysis (17.885%) and yield (9.113%). Notably, while initial screenings (Minimum Inhibition Concentration and Minimum Bactericidal Concentration assays) at 64–256 mg/mL shown no inhibitory activity, the 6-hour hydrolysate at a higher concentration of 1000 mg/mL demonstrated bacteriostatic activity. Using the disc diffusion method, this treatment produced inhibition zones of 1.735 mm against Staphylococcus aureus and 1.330 mm against Escherichia coli. These findings suggest that while tuna viscera protein hydrolysates possess antibacterial potential, their efficacy is highly dependent on both hydrolysis duration and concentration, providing a foundation for future valorization of fish processing by-products.
Co-Authors A. Shofy Mubarak Abdillah, Annur Ahadi Abdul Azis Abidin, Faris Agustono - Ahmad Zainal Fanani Alfi Hermawati Waskita Sari Alief Ayu Selia Alvianita Az-Zahra, Naela Amin, Muhamad Nur Ghoyatul Annisa, Amelia Nur Apriliyanto, Mochammad Bayu Arum, Kartika Dwi Sekar Bayu Kanetro Catlya Tasya Adella Tasya Adella Dewi, Siti Nur Rukhoiyah Dita Wisudyawati Duanty, Putri Valentina Dwi Anggorowati Rahayu Dwi Yuli Pujiastuti Dwitha Nirmala Dwiyati Pujimulyani Eka Saputra Emy Koestanti Sabdoningrum Endang Dewi Masithah Fanani, Ahmad Zainal Fareza Ferdyna Nanda Sumartono Faris Abidin Fauziah, Aghnina Elvia Firmansyah, Muhammad Ichwan Hafizh M. Noor Haris, Mochamad Husein, Mohamad Akmal Alwi Islam, Nuruddin Haquel Janah, Diana Roudhotul Lailatul Lutfiyah, Lailatul Larasati, Melani Mahrunisa, Aulia Dista Maulidia, Cici Miftakhul Ulum, Miftakhul Mufasirin N. Juni Triastuti Nova Andika Novitasari, Tisa Ayu Nuning Vita Hidayati Nur Aida Harahap Nur Izzati Mohd Noh Nuriyansyah Pangestu, Chandra Trisna Patmawati Patmawati, Patmawati Pipin Suciati Pipin Suciati Pipin Suciati Pulung Siswantara Purnama, Diva Frida Riesta Pursetyo, Kustiawan tri Putri, Monica Angelina Ramadhan, Novelix Arditan Rofi’ah, Siti Choiriah Rohmah, Arina Maulidatur Rohmatin, Aulia Rokhim, Akhmad Nur Sahidu, Adriana Monica Sapto Andriyono Siti Choiriah Rofi’ah Siti Eliana Siti Nur Rukhoiyah Dewi Siti Sahatul Fatimah Sofi Maulana Sri Utari Suciyono, Suciyono Sumartono, Fareza Ferdyna Nanda Tanti Dwi Oktavia Taruna Fernando Putra Tisa Ayu Novitasari Tjahjaningsih, Wahju Tri Wahyuni tri wahyuni Veryl Hasan Wahju Thajaningsih Wahyu Ardianto Wahyu Ardianto Warsito, Sunaryo Hadi Widiana, Hanidhan Dwiretno Wina Sapphira, Eunike Wulan, Novrida Yuli Pujiastuti, Dwi