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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JFMR (Journal of Fisheries and Marine Research) Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Grouper: Jurnal Ilmiah Perikanan Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Proceedings Series on Physical & Formal Sciences Amkop Management Accounting Review (AMAR) Journal of Marine Resources and Coastal Management Balobe: Jurnal Pengabdian Masyarakat Journal of Fisheries & Marine Journal of Food and Agricultural Technology
Heru Pramono
Department Of Marine, Faculty Of Fisheries And Marine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Social Sciences Veterinary Other

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Effects of culture conditions on proteolytic activity of Staphylococcus saprophyticus isolated from a traditional fermented condiment Duanty, Putri Valentina; Pramono, Heru; Nirmala, Dwitha
Journal of Marine Resources and Coastal Management Vol. 7 No. 1 (2026)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v7i1.2433

Abstract

Madura petis is a traditional fermented condiment from East Java, Indonesia, made from shrimp or fish cooked with sugar to produce a rich, savory paste. Its characteristic umami flavour develops naturally during storage through spontaneous fermentation involving proteolytic microorganisms. The aims of the study were to analyze the production of protease in different culture condition and nutrition of Staphylococcus saprophyticus (strain PMB1-7) isolated from petis. The isolate was a Gram-positive, catalase-positive coccus that does not ferment mannitol and is resistant to novobiocin, a typical of S. saprophyticus. Growth profiling revealed it reached stationary phase after 12 h of culture. Protease activity assays were conducted under different media, pH levels, and incubation times to determine optimal enzymatic conditions. The highest proteolytic activity occurred in Nutrient Broth (proteolytic index: 1.09 ± 0.01), at neutral pH (1.22 ± 0.10), and after 24 hours of incubation (1.20 ± 0.00). These results offer valuable insights into the enzymatic behavior of S. saprophyticus during petis fermentation and provide a foundation for developing controlled fermentation approaches to enhance the flavour quality of traditional Indonesian condiments.
Probiotic Biotechnology Innovation in Tiger Grouper Aquaculture and Its Implications for Business Economic Performance Ratri, Kartika Chayaning; Satyantini, Woro Hastuti; Pramono, Heru
Amkop Management Accounting Review (AMAR) Vol. 6 No. 1 (2026): January - June
Publisher : Sekolah Tinggi Ilmu Ekonomi Amkop Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37531/amar.v6i1.3675

Abstract

The development of aquaculture technology has become an important strategy in increasing fish production while maintaining environmental sustainability. One of the innovations increasingly applied in aquaculture systems is the use of probiotics, which function to improve fish health, enhance water quality, and increase cultivation efficiency. Tiger grouper (Epinephelus fuscoguttatus) is a high-value marine commodity with significant economic potential; however, its cultivation still faces several challenges, including disease outbreaks, low feed efficiency, and unstable environmental conditions. Therefore, the application of probiotic biotechnology is considered a promising approach to address these challenges. This study employed a literature review method by analyzing various international scientific publications related to the application of probiotics in aquaculture systems. The selected literature focused on three main aspects: the application of probiotic biotechnology in tiger grouper cultivation systems, the effect of probiotics on production performance, and the economic implications of probiotic use in aquaculture businesses. The results of the literature synthesis indicate that probiotics play a significant role in improving microbial balance in both the digestive system of fish and the rearing environment. The application of probiotics has been reported to enhance fish growth rate, increase survival rate, and improve feed utilization efficiency. In addition, probiotics contribute to maintaining water quality by reducing harmful compounds such as ammonia and organic waste in aquaculture systems. Overall, the findings highlight that probiotic biotechnology innovation not only improves the biological and technical performance of tiger grouper cultivation but also provides economic benefits through increased productivity, reduced operational costs, and improved product quality. Therefore, probiotic application can be considered a strategic approach to support sustainable and economically viable aquaculture development.
Co-Authors A. Shofy Mubarak Abdillah, Annur Ahadi Abdul Azis Abidin, Faris Adriana Monica Sahidu Agustono - Ahmad Zainal Fanani Alfi Hermawati Waskita Sari Alief Ayu Selia Alvianita Az-Zahra, Naela Amin, Muhamad Nur Ghoyatul Annisa, Amelia Nur Apriliyanto, Mochammad Bayu Arum, Kartika Dwi Sekar Bayu Kanetro Dewi, Siti Nur Rukhoiyah Dita Wisudyawati Duanty, Putri Valentina Dwi Yuli Pujiastuti Dwitha Nirmala Dwiyati Pujimulyani Eka Saputra Emy Koestanti Sabdoningrum Endang Dewi Masithah Fanani, Ahmad Zainal Fareza Ferdyna Nanda Sumartono Faris Abidin Fauziah, Aghnina Elvia Firmansyah, Muhammad Ichwan Hafizh M. Noor Haris, Mochamad Husein, Mohamad Akmal Alwi Islam, Nuruddin Haquel Larasati, Melani Mahrunisa, Aulia Dista Maulidia, Cici Miftakhul Ulum, Miftakhul N. Juni Triastuti Nova Andika Novitasari, Tisa Ayu Nuning Vita Hidayati Nur Aida Harahap Nuriyansyah Pangestu, Chandra Trisna Patmawati Patmawati, Patmawati Pipin Suciati Pipin Suciati Pipin Suciati Pulung Siswantara Purnama, Diva Frida Riesta Pursetyo, Kustiawan tri Putri, Monica Angelina Ramadhan, Novelix Arditan Ratri, Kartika Chayaning Rofi’ah, Siti Choiriah Rohmatin, Aulia Rokhim, Akhmad Nur Sapto Andriyono Siti Choiriah Rofi’ah Siti Eliana Siti Nur Rukhoiyah Dewi Siti Sahatul Fatimah Sofi Maulana Sri Utari Suciyono, Suciyono Sumartono, Fareza Ferdyna Nanda Tanti Dwi Oktavia Taruna Fernando Putra Tisa Ayu Novitasari Tjahjaningsih, Wahju Tri Wahyuni tri wahyuni Veryl Hasan Wahju Thajaningsih Wahyu Ardianto Wahyu Ardianto Warsito, Sunaryo Hadi Widiana, Hanidhan Dwiretno Wina Sapphira, Eunike Woro Hastuti Setyantini Wulan, Novrida Yuli Pujiastuti, Dwi