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I Made Dwinata
Labolatorium Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana. Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Protein Spesifik Cairan Kista Cysticercus bovis pada Sapi Bali yang Diinfeksi dengan Taenia saginata (SPECIFIC PROTEIN OF CYSTICERCUS BOVIS CYST FLUID ON BALI CATTLE EXPERIMENTALLY INFECTED WITH TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; Kadek Swastika; I Made Damriyasa; Ida Bagus Made Oka; I Nyoman Mantik Astawa
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cysticercus bovis is the larval stage of Taenia saginata, the bovine tapeworm. The infection of thislarval in cattle musculature causes Bovine cysticercosis or Cysticercosis bovis.  Bovine cysticercosis is foundworldwide, but mostly in developing countries, where unhygienic conditions, poor cattle managementpractices, and the absence of meat inspection are common.  The adult Taenia infection in man is referredto as taeniasis.  Taenia saginata taeniasis is also found almost all over the world.  The prevalence ofTaenia saginata taeniasis has reported up to 27.5% in Gianyar Bali. In order to control the diseases,vaccination against the larvae stages in cattle of Taenia saginata may play an important role in controllingthe disease in the endemic regions.  The aims of the present study were to prepare and to investigate theimmunogenic protein as vaccine candidate for controlling  Cysticercus bovis infection in in Bali cattle.Cysticercus protein from the cyst fluid was firstly used to immunize mice and the mice sera were thencollected. Cysticercus proteins then analyzed using sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE).All cysticercus proteins were then visualized by Commasie blue staining. The proteins were also transferredonto nitrocellulose membrane and the immunogenic proteins were visualized by Western Blotting usingimmune sera raised in mice.  By Commasie blue staining, a total of 17 proteins were detected with themolecular weight of 14,86 kDa -122,40 kDa from the smallest to the largest. As many as 7 immunogenicproteins with the molecular weights of 16.81 kDa; 19.22 kDa; 20.98 kDa; 27.41 kDa; 34.02 kDa; 38.31 kDa;and 54.94kDa were detected.
Seroprevalensi dan Isolasi Toxoplasma gondii pada Ayam Kampung di Bali (SEROPREVALENCE AND ISOLATION OF TOXOPLASMA GONDII AMONG FREE-RANGE CHICKENS IN BALI) I Made Dwinata; Ida Bagus Made Oka; Nyoman Adi Suratma; I Made Damriyasa
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T.gondii oocysts in the environment and the meat of chicken is considered one of the sources of the humaninfection. A study to determine the seroprevalence of T.gondii in free ranging chickens in eight regency inBali have been undertaken. More over, attempt to isolate T gondii was also performed from the copropositivesample. Seroprevalence was detected using modified agglutination test (MAT) and isolation of T.gondiiwere performed from organs (heart and brain) using pepsin-HCl digestion method. Further the pathogenicityof the isolate was determined by bioassay using mice. The result showed that the seroprevalence was24.8% (31 out of 125 chickens examined). T.gondii was found in 17 of the 31 seropositive chickens (55%)more over all isolates were a vitulent to the mice.
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Deteksi Antibodi dan Isolasi Toxoplasma gondii pada Itik lokal di Bali (DETECTION ANTIBODIES AND ISOLATION OF TOXOPLASMA GONDII IN DOMESTIC DUCK IN BALI ) Made Dwinata; Ida Bagus Made Oka; I Made Damriyasa
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to determine detection antibodies and isolation T. gondii infection in domestic duck in Bali. A total, 188 domestic ducks sera were examined using indirect haemaglutination test kit (IHA). Heart, brain and muscle of seropositive IHA test were used for isolation with pepsin-HCL digestion and bioassay in mice and cat. The result of these research showed that 47 (25%) ducks were found to be positive for T. gondii antibodies at the cut-off e” 1:64. The seroprevalence in male and female duck were 27,8% and 22,4% respectively, however, statistical analysis showed that the difference was not significant (P>0,05). The seroprevalence in cage and free-range duck were 18,7% and 29,2% respectively, but the difference was not statistically significant (P>0,05). The antibodies titer ranging from 1:64 to 1:2048. Also, viable T. gondii was isolated from seropositive duck by bioassay mice and cat. Most of the isolated strains were avirulent to mice. This study showed that domestic duck could have a potensial role in transmitting toxoplasmosis to human in Bali.
Identifikasi Jenis Lalat Tidak Penghisap Darah pada Peternakan Babi di Bali Alice Viria Cordeiro da Costa Xavier; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p13

Abstract

Flies are ectoparasites that act as vectors of disease and their existence greatly affects the health of pigs. The purpose of this study was to identify the types and fluctuations in the number of flies that do not suck blood in pig farms in Badung Regency and Denpasar City. The fly collection was carried out on a semi-intensive pig farm with a period of every week for 3 months. In this study, flies were caught using fly paper glue and sweeping net and then euthanized with 70% alcohol. The data obtained were presented descriptively and analyzed using the Wilcoxon test. The identification results showed that the type of non-blood-sucking flies found in pig farms in Badung Regency and Denpasar City was Musca sp. as many as 2,383 tails (96.99%), Lucilia sp. as 56 tails (2.28%) and Fannia sp. as 18 tails (0.73%). During the 12 weeks of observation, fluctuations occurred with the highest decrease in fly population in the 3rd week (November) and 10th (January) while the increase occurred in the 5th week (December) to the 9th week (January). The number of fly population found in the Denpasar pig farm is 1,464 larger, which is largerthan the population in the pig farm in Badung Regency 993 tails.
Prevalensi Infeksi Isospora spp. pada Kucing di Kota Denpasar Nelviana Mesquita; Nyoman Adi Suratma; I Made Dwinata
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p18

Abstract

Cats are one of the most popular pets in the world with a wide variety of breeds, and as pets that get attention to be kept and bred.The common gastrointestinal protozoa that infect cats are Gardia felis, Cryptosporidium felis, Isospora felis, Isospora rivolta, Toxoplasma gondii, Hammondia hammondi and Sarcocystis sp., while Pentatrichomonas hominis can infect the large intestine. This study aims to determine the prevalence of infection and the relationship between some factors (breed, sex, age, and maintenance management) on gastrointestinal protozoa in cats in Denpasar. This study involved 100 samples of cat feces and the data were analyzed by chi-square. Samples were examined using the floating concentration method, with a saturated NaCl flotation agent. The results showed that the prevalence of gastrointestinal protozoa infections in cats reached 24% (24/100). Breed, sex, age and maintenance management factors were not associated with the prevalence of gastrointestinal protozoa infections in cats.
Prevalensi Infeksi Cacing Gastrointestinal Berpotensi Zoonosis pada Kucing di Kota Denpasar Makrina Weni Misa; Nyoman Adi Suratma; I Made Dwinata
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p03

Abstract

Cats as pets have their own charm because of their various body shapes, eyes and hair colors. However, cats are susceptible to disease, and even become a source of zoonoses for humans. There are several types of worm parasites that are often found in the digestive tract of cats such as Ancylostoma spp., Toxocara cati, Strongyloides spp. Dipylidium caninum and Spirometra which have high potential to cause zoonoses. The purpose of this study was to determine the prevalence of infection and the relationship with several factors (race, sex, age and maintenance management) to gastrointestinal worms that have zoonotic potential in cats in Denpasar City. The number of samples used in this study was 100 samples of cat feces and the data were analyzed by chi-square test. Samples were examined using the floating concentration method, with a saturated NaCl flotation agent. The results of the study found that the prevalence of Ancylostoma spp. was 8%, Toxocara cati was 10% and there was a mixed infection between the two of 19%. So, the overall percentage is 37%. Meanwhile, it is also known that there is a relationship between maintenance management and the prevalence of gastrointestinal helminth infections in cats. On the other hand, there is no relationship between race, sex, and age as risk factors with the prevalence of gastrointestinal worms.
Prevalensi dan Identifikasi Ektoparasit pada Anjing Kintamani Bali di Bali Ni Wayan Nur Sidi Murti; Ida Bagus Made Oka; I Made Dwinata
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p17

Abstract

Ectoparasites are parasites that live parasitically on the outside of the host's body or tunnels in the skin that are still in contact with the external environment. Impacts caused by ectoparasite infestations in dogs include decreased body weight, decreased production, hair loss, irritation, anemia and even death. The purpose of this study was to determine the prevalence of infestation and identification of ectoparasites that infest the Anjing Kintamani Bali (AKB). The AKB samples used were 100 individuals. The Anjing Kintamani Bali selected as the sample tested positive if ectoparasites were found in the dog. Identification of ectoparasites based on morphology. The data obtained were analyzed by Chi-square test. The results showed that the prevalence of ectoparasites in AKB in Bali was 56%. The types of ectoparasites that infest include: Riphicephalus sanguinus, Ctenocephalides felis, Heterodoxus spiniger and Trichodectes canis. Chi-square test found that maintenance methods were associated with the prevalence of ectoparasite infestations in AKB in Bali, while age and sex were not correlated. Maintenance of the Anjing Kintamani Bali in Bali, you should pay more attention to animal health. Regular bathing of animals is highly recommended to control ectoparasite infestations, besides that the owner can comb the animals regularly. Sanitation of cages for animals in cages is very important to pay attention to, besides that, it is better for animals that have been severely infested to be taken to the nearest veterinarian.
Prevalensi Infeksi Koksidia pada Anjing Kintamani Bali Luh Gede Winda Maheswari; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p18

Abstract

Coccidiosis is an infectious disease of the digestive tract in dogs that can cause digestive disorders. The purpose of this study was to determine the prevalence of coccidia infection and its relationship with risk factors (age, sex, maintenance system) in Kintamani Bali Dogs. Sampling was carried out in several areas in Bali, namely Denpasar City, Gianyar Regency and Sukawana Village, Kintamani District, Bangli Regency by purposive sampling. The number of research samples was 150 faecal samples and examined using the floating method. The data obtained were analyzed by chi-square test. The results showed that from 150 samples of Kintamani Bali Dog feces examined, 5 samples were positive for Coccidia infection with a prevalence of 3.33%, 2 samples were identified as infected with Eimeria spp. 1.33% (2/150) and 3 samples infected with Isospora spp. 2% (3/150). Analysis by Chi-square test there was no significant relationship between age, sex and maintenance system on the prevalence of coccidia infection in Kintamani Bali Dogs. The owner of the Kintamani Bali Dog should pay more attention to maintenance, both from feeding, cleaning the cage and also administering protozoan drugs to reduce the prevalence of Coccidia infection.
Co-Authors Affan Nur Alamsyah, Affan Nur Agus manahan manik Alice Viria Cordeiro da Costa Xavier Anak Agung Gde Arjana Anak Agung Gde Oka Dharmayudha Anak Agung Wisnu Kusuma Putra Ariawan, Kadek Yudha Astuti, K.W. Ayu Talia Shalsa Billa Bellantari, Melinda Chairannisa Rustam Cok Krishna Pemayun Desyandri Desyandri Dhea Septiany Peda Lalupada Dina, Putu Ayu Endah Rahmawati Evayana, Made Fajar Mubarok, Fajar Gilang Kala Maulana, Gilang Kala Here, Rischi Robinson Male Heri Utomo Baihaqi, Heri Utomo I Kadek Swastika I Ketut Puja I Made Damriyasa I Made Galih Diparayoga I Made Kardena I Made Merdana i Nengah Wandia I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I PUTU YOGA LOKANTARA I Wayan Batan I Wayan Sudira Ida Ayu Pasti Apsari Ida Bagus Made Oka Kadek Ayu Dwi Suryastini Kadek Febriana Marta Putra Kadek Karang Agustina Ketut Budiasa Lestari, Maureen Tyas Luh Dewi Anggreni Luh Gede Winda Maheswari Makrina Weni Misa Maria Mentari Ginting, Maria Mentari Mariyana, Lilik Dwi Mersy Rambu Maramba Ndiha Muhammad Gustav Satriadistfa Septiadi, Muhammad Gustav Mukti, Taufik Muliani, Ni Kadek Nelviana Mesquita NI LUH PUTU SHISTA PAWITRI Ni Nyoman Widiasih Ni Wayan Nur Sidi Murti Nonitema Nazara PANDE KETUT YULIARI Pratiwi, Dede Ayu Putra, B.P.A. Putu Anna Oktaviana Putu Sita Paramita Diyani Putu Suma Githa Sanjaya Putu Titin Evi Sucitrayani Rahma Anissa Rendra Ari Purna, Rendra Ari Saduarsasila, I Gede Agus Kana Sajuri, Indri Agustin Stevi SAMUYUS NEALMA Septianingsih, Ni Luh Putu Diah Simamora, Saruedi Siswanto, Riefqy Tepu Suandhika, Putu Wiweka, Ade Hary Yoseph, Veronica Vriscilla