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Potensi Jenis Tumbuhan Bawah Berkhasiat Obat di Hutan Kota Raggawulung, Kabupaten Subang Dina Anggraini; Lily Surayya Eka Putri; Dasumiati Dasumiati
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The objective of this research was to know the understory species diversity and to identified the potency of medicinal plants in Ranggawulung Urban Forest. The method of this research is vegetation analysis using circular plot (4 m in diameter), we used 30 plot in the site. We counted number of individuals and species richness. Importance Value Index, Simpson’s, and Shanon-Wiener Index was calculated to describe it’s diversity. The result showed Ranggawulung Urban Forest had high of understory diversity (Shanon-Wiener Index 3,36). There was 56 understory species, and 19 of them was identified as medicinal plants. There are Ageratum conyzoides, Piper aduncum, Sida rhombifoli, Eupatorium odoratum, and Turnera ulmifolia. The utilization of this potency and the effort to conserve both ex-situ and insitu are also elaborated.

Optimalisasi Penggunaan Pupuk Kompos dengan Penambahan Effective Microorganism 10 (Em10) pada Produktivitas Tanaman Jagung (Zea mays L.) Elpawati Elpawati; Stephani Dwi Dara; Dasumiati Dasumiati
Al-Kauniyah: Jurnal Biologi Vol 8, No 2 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

This research is aimed to determine the effect of growing media composition, the addition different concentration of EM10 fertilizer, and interaction between the composition of the growing media with different concentration of EM10 fertilizer on Zea mays growth and productivity. This research was conducted in Home Composting of UIN Syarif Hidayatullah Jakarta from June until October 2013. This study used Random Grouped for Factorial Design 2 x 5 wth 3 repetitions. First factor was plant media dose (M), consist of two extents, M1 (compost:soil = 1:1) and M2 (compost:soil = 1:2). The second factor was fertilizer concentration (D), consist of five extents, DO (no fertilizer),D1 (Urea: 0,9 g, SP-36: 0,9 g, KCl: 0,45 g), D2 (10 ml EM10), D3 (15 ml EM10), and D4 (20 ml EM10). The obtained data was analyzed using Analysis of Varians(ANOVA) and Duncan test when there is a significant difference..The result showed plant media (M2) with ratio composition of compost and soil was 1:2 could increase the stem diameter at harvest time. 20 ml concentration of EM10 could increase the stem diameter’s growth (2.29 mm) at harvest time, similarly 15 ml EM10 could increase the cob’s productivity (1.66 cobs). The interaction of composition plant media and fertilizer concentration of EM10 did not influence corn’s growth and productivity.

Quality of Elephant Grass (Pennisetum purpureum Schumach.) Gamma Irradiation Result in the Second Generation (MV2) Manhalush Intan Shafifi; Dasumiati Dasumiati; Deudeu Lasmawati; Marina Yuniawati Maryono; Junaidi Junaidi; Irawan Sugoro
Al-Kauniyah: Jurnal Biologi Vol 15, No 1 (2022): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i1.17008

AbstrakRumput gajah (Pennisetum purpureum Schumach.) memiliki kadar protein kasar 6,26%. Ternak ruminansia membutuhkan protein kasar 10–15%. Cara alternatif untuk meningkatkan kadar protein kasar dan karakter agronomi dapat dilakukan dengan metode mutasi induksi menggunakan sinar gama. Penelitian ini bertujuan untuk mengetahui kadar protein kasar tertinggi dan melakukan karakterisasi aksesi hasil iradiasi gama untuk proses seleksi. Rancangan percobaan yang digunakan, yaitu rancangan acak kelompok faktorial. Faktor pertama adalah iradiasi dosis dengan taraf 0, 10, 20, 30, 40, dan 50 Gy. Faktor kedua adalah buku batang, yaitu buku batang atas, tengah, dan bawah. Hasil penelitian menunjukkan bahwa protein kasar tertinggi terjadi pada perlakuan buku tengah dengan dosis 50 Gy dengan nilai 17,21% dan peningkatan persentase sebesar 12,19%. Hasil berpengaruh nyata pada generasi kedua terjadi pada karakter agronomi jumlah tunas. Kadar protein kasar tertinggi terjadi pada buku tengah dosis 50 Gy. Perlakuan buku atas dosis 10 Gy memperoleh karakter jumlah tunas yang banyak pada generasi kedua. Peningkatan kadar protein kasar dan karakter agronomi rumput gajah yang diperoleh belum stabil, karena termasuk dalam generasi awal. Hasil penelitian ini dapat digunakan sebagai penelitian lanjutan untuk memperoleh tanaman rumput gajah dengan kadar protein kasar dan karakter agronomi rumput gajah yang stabil.Abstract Elephant grass (Pennisetum purpureum Schumach.) had crude protein 6.26%. Ruminant animals need 10–15% crude protein. An alternative way to increase the crude protein with induction by used gamma-ray irradiation. The purpose of this research were to determine the highest crude protein content and characterize accessions resulting from gamma irradiation for the selection process. The experimental design used a random block design. The first factor, gamma irradiation with level dose 0, 10, 20, 30, 40, and 50 Gy. The second factor, upper node, middle node, and lower node. The result were the highest crude protein occurred in the middle node with a dosage of 50 Gy with a value of 17.21% and a percentage increase 12.19%. The significant result occurred in agronomic characters of total of shoots in the second generation. The highest increase of crude protein occurred on the middle node dose of 50 Gy. Interaction of the upper node dose of 10 Gy was able to obtain the most shoots in the second generation. The increase in crude protein content and agronomic character of elephant grass was not stable because it was included in the early generation. The results of this research can be used as further research to obtain elephant grass with stable high crude protein content and stable elephant grass agronomic characters.

Nilai Duga Keragaman Genetik, Heritabilitas, dan Korelasi antar Karakter Mutan Rumput Gajah Generasi MV3 Heny Hermawati; Marina Yuniawati Maryono; Dasumiati Dasumiati; Junaidi Junaidi; Irawan Sugoro
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 18, No 1 (2022): DESEMBER 2022
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17146/jair.2022.18.1.6472

Tanaman rumput gajah (Cenchrus purpureus (Schumach.) Morrone) hasil iradiasi sinar gamma yang unggul dan stabil secara genetik dapat diperoleh melalui seleksi. Mutan rumput gajah generasi MV1 dan MV2 belum stabil secara genetik sehingga perlu dilakukan seleksi pada generasi MV3. Seleksi merupakan salah satu tahapan pemuliaan tanaman untuk perbaikan karakter dan dapat dilakukan berdasarkan parameter genetik, yaitu keragaman genetik, heritabilitas, dan korelasi antar karakter. Tujuan penelitian adalah untuk mengetahui nilai duga keragaman, nilai duga heritabilitas, dan korelasi antar karakter mutan rumput gajah generasi MV3. Penelitian dilakukan menggunakan sampel mutan rumput gajah generasi MV3 dengan 18 perlakuan hasil iradiasi (B1D0, B1D1, B1D2, B1D3, B1D4, B1D5, B2D0, B2D1, B2D2, B2D3, B2D4, B2D5, B3D0, B3D1, B3D2, B3D3, B3D4, B3D5) pada generasi MV2 dan 3 ulangan. Hasil penelitian menunjukkan bahwa nilai duga keragaman genetik tergolong rendah, sedang, dan tinggi, sedangkan nilai duga heritabilitasnya rendah dan sedang. Koefisien korelasi antar karakter mutan rumput gajah generasi MV3 menunjukkan hasil positif dengan derajat keeratan hubungan lemah, sedang, kuat, dan sangat kuat. Keragaman genetik, heritabilitas, dan korelasi dengan nilai sedang–tinggi (kuat) terdapat pada karakter jumlah daun, jumlah buku batang, berat segar, kandungan bahan kering, abu, dan bahan organik. Karakter tersebut dapat dijadikan sebagai karakter seleksi sehingga berguna untuk acuan dasar proses seleksi mutan rumput gajah generasi MV3.

Forage-Yield and Nutrient Quality of New Brown Midrib (BMR) Mutant Lines of Sorghum T. Wahyono; W. M. Indriatama; W. T. Sasongko; E. N. Shafira; T. F. Hidayat; S. Widodo; Y. Widiawati; M. N. Rofiq; Y. N. Anggraeny; A. Kurniawati; Dasumiati
Tropical Animal Science Journal Vol. 46 No. 1 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.1.63

The objective of this study was to evaluate the yield, nutrient profile and in vitro digestibility of new BMR mutant lines of sorghum in Indonesia. These mutant lines were GH2.1, GH2.2, GH2.3, GH4.1, GH4.2, GH4.3 and GH4.4. One sorghum mutant line (CTY) and two national sorghum varieties (Super 1 and Bioguma) were also evaluated as controls. In vitro digestibility and rumen fermentation were measured using Ankom Daisy Fermenter and Hohenheim gas test methods, respectively. In vitro measurement consisted of ten treatments with five replications following a completely randomized design. The highest stem sugar content was found in Bioguma (11.22%) and GH4.4 (9.32%) (p<0.05). The Bioguma variety and the GH2.3 mutant line had a higher number of stem segments and fresh forage yield than the Super 1 variety (p<0.05). A greater concentration of crude protein (CP) was observed for the GH.2.1, GH2.2, GH2.3 and GH4.1 lines (p<0.05). The GH2.3 mutant line had the lowest acid detergent lignin (ADL) content (p<0.05), while Bioguma had the highest level of non-fibre carbohydrate (NFC) compounds (p<0.05). The highest relative feed value (RFV) was observed for the GH2.3 line (p<0.05). Furthermore, GH4.2 and GH2.3 had greater in vitro true digestibility (IVTD) (p<0.05) but were not significantly different from Bioguma. Regarding yield characteristics, nutrient composition and in vitro digestibility values, the highest values were found in the Bioguma variety and the GH2.3 mutant line. Except for n-valerate (nC5), significant differences in all rumen fermentation parameters were observed among sorghum cultivars (p<0.05). Regarding the interrelationship between parameters, we found a medium correlation of DMD with the ADL and cellulose content of sorghum forage (R2 = -0.489 and R2 = -0.674, respectively). Based on these findings, the GH2.3 BMR mutant line should be further developed as forage sorghum.

Morfologi Daun Durian (Durio zibethinus L.) dan Keanekaragaman Genetiknya Berdasarkan Marka ISSR Fitri Annisa; Priyanti Priyanti; Dasumiati Dasumiati
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.23975

AbstrakData keanekaragaman durian (Durio zibethinus L.) yang terbatas merupakan salah satu kendala yang dihadapi dalam pengelolaan dan pengembangannya di Indonesia. Keanekaragaman genetik dapat ditinjau berdasarkan ciri morfologi yang didukung dengan ciri molekuler. Tujuan penelitian ini adalah untuk mengetahui kekerabatan antar aksesi berdasarkan dendrogram yang menggabungkan data morfologi daun dan polimorfisme Inter Simple Sequence Repeats (ISSR). Pengamatan ciri morfologi mengacu pada deskriptor durian dan ektraksi DNA dengan metode CTAB yang telah dimodifikasi. Setelah berhasil diekstraksi, DNA durian diamplifikasi dengan tujuh primer ISSR untuk analisis polimorfisme. Konstruksi dendrogram yang terbentuk merupakan hasil analisis menggunakan program NTSYS. Berdasarkan 15 ciri morfologi yang diamati terdapat 5 ciri yang sangat membedakan antara kelompok aksesi durian. Lai (D. kutejensis) memiliki daun lebih panjang hingga 24 cm dan lebar hingga 16 cm dibandingkan 28 aksesi durian dengan panjang hingga 16 cm dan lebar hingga 9 cm. Hasil amplifikasi diperoleh sebanyak 35 pita dan 31 pita bersifat polimorfik dengan persentase polimorfisme berkisar antara 66,7–100%. Persentase polimorfisme pada penelitian ini dapat mencapai 100% dengan primer ISSR 842, PKBT 4, dan PKBT 5. Konstruksi dendrogram ciri morfologi mempunyai koefisien kemiripan sebesar 0,56–0,91 dan membentuk dua kelompok yang tidak memisahkan aksesi-aksesi durian dengan Lai. Ciri polimorfisme ISSR dapat digunakan untuk merekonstruksi dendrogram menjadi dua kelompok dengan koefisien kemiripan sebesar 0,48–0,96. Dendrogram ciri polimorfisme ISSR secara tegas memisahkan aksesi-aksesi durian dengan Lai. Pohon kekerabatan 28 aksesi durian dan Lai di Kecamatan Serpong telah direkonstruksi untuk pertama kalinya.AbstractLimited data on the diversity of durian (Durio zibethinus L.) is one of the obstacles encountered in its management and development in Indonesia. Genetic diversity can be reviewed based on morphological characteristics supported by molecular characteristics. This study aimed to determine the relationship between accessions based on a dendrogram that combines leaf morphology and Inter Simple Sequence Repeats (ISSR) polymorphism data. Observation of morphological features refered to durian descriptors and DNA extraction using the modified CTAB method. After successful extraction, durian DNA was amplified with seven ISSR primers for polymorphism analysis. The dendrogram construction is formed the NTSYS program. Based on the 15 morphological characteristics observed, 5 characteristics greatly distinguished the durian accession group. Lai (D. kutejensis) had longer up to 24 cm and wider up to 16 cm leaves than 28 durian accessions with a length (16 cm) and width (9 cm). The amplification results were obtained 35 bands and 31 out of them were polymorphic with polymorphic PCR product ranged 66.7–100%. The percentage of polymorphism could reach 100% with ISSR 842, PKBT 4, and PKBT 5 primers. The dendrogram construction based on morphological characteristics had an interaction coefficient of 0.56–0.91 and formed two groups which did not separate durian accessions with Lai. The ISSR polymorphism feature can be used to reconstruct the dendrogram into two groups with a slope coefficient of 0.48–0.96. The dendrogram based on the ISSR polymorphism feature explicitly divides durian accessions with Lai. The cluster analysis of 28 durians and Lai accessions in Serpong District has been reconstructed for the first time.

MICROPROPAGATION OF POTATO (Solanum tuberosum L.) cv. GRANOLA IN LIQUID MEDIUM USING AERATION SYSTEM FOR G0 SEED PRODUCTION Karyanti Karyanti; Hayat Khairiyah; Tati Sukarnih; Yayan Rudiyana; Isni Nasrifah; Aida Wulansari; Shinta Magdalena Septiani; Dasumiati Dasumiati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

ABSTRACT Disease-free potato seeds of high quality can be obtained via in vitro culture. The use of liquid medium during in vitro cultures might boost the number of plantlets produced, however, the problem of hyperhydricity in plantlets was often encountered. This study aimed to investigate effects of different sucrose concentrations and application of aeration system on micropropagation of potato cv. Granola using liquid medium. Aseptic nodal explants with 3-4 nodes from established in vitro cultures were subjected to MS liquid medium with a factorial treatment of three sucrose concentrations (0, 7.5, 15 g. L-1) and two culture (with and without aeration). The results showed that MS medium with 7.5 g L-1 sucrose was the best medium to produce the highest number of shoots and nodes. Furthermore, it was found that application of aeration system in MS liquid culture decreased plantlet hyperhydricity and increased the number of shoots, number nodes, plantlet height, as well as improved plantlet morphology and vigor. Application of the aeration system in liquid medium produced 200-230 new potato plants per bioreactor in the acclimatization stage and an average of 2773.5 G0 mini tubers. ABSTRAK Benih kentang bebas penyakit berkualitas tinggi dapat diperoleh dari kultur in vitro. Penggunaan media cair selama kultur in vitro dapat meningkatkan jumlah planlet yang dihasilkan, namun masalah hiperhidrisitas plantlet masih sering dijumpai. Penelitian ini bertujuan mengetahui pengaruh perbedaan konsentrasi sukrosa dan penerapan sistem aerasi terhadap perbanyakan kentang cv. Granola menggunakan media cair. Eksplan dengan 3-4 nodus dari kultur in vitro ditumbuhkan pada media cair MS dengan perlakuan faktorial, faktor pertama: tiga tingkat konsentrasi sukrosa (0, 7,5, 15 g. L-1) dan faktor kedua: dua sistem kultur (dengan dan tanpa aplikasi aerasi). Hasil menunjukkan bahwa media MS dengan 7,5 g. L-1 sukrosa merupakan media terbaik untuk menghasilkan jumlah tunas dan nodus terbanyak. Selanjutnya ditemukan bahwa penerapan sistem aerasi pada kultur cair MS menurunkan hiperhidrisitas plantlet dan meningkatkan jumlah tunas, jumlah buku, tinggi plantlet, serta memperbaiki morfologi dan vigor plantlet. Penerapan sistem aerasi pada media cair menghasilkan 200-230 tanaman kentang baru per bioreaktor dalam tahap aklimatisasi dan rata-rata 2773,5 G0 umbi mini.

ROOT INDUCTION ON THE SHOOTS OF RUBBER TREE (Hevea brasiliensis) CLONE PB 260 THROUGH EX VITRO TECHNIQUE Yusuf Sigit Ahmad Fauzan; . Dasumiati; Dewi Rizkiani; Syofi Rosmalawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5363

Rubber tree (Hevea brasiliensis) is a crucial plantation commodity for Indonesia's national development. However, the country's rubber productivity lags behind that of other rubber-producing nations. To address this, propagating rubber plants from superior clones through ex-vitro techniques becomes essential.. This study aimed to determine the optimal concentrations of IBA and NAA for root induction in rubber tree shoot explants. A factorial complete randomized design was employed, with the first factor being IBA concentration (0, 100, 200, 300, 400, and 500 ppm) and the second factor being NAA concentration (0, 50, 100, and 150 ppm). The results revealed that the percentage of surviving explants after induction with IBA and NAA did not significantly differ. The rooted explants were obtained using the following treatments: IBA 200 ppm and NAA 0 ppm, IBA 200 ppm and NAA 50 ppm, IBA 300 ppm and NAA ppm, and IBA 400 ppm and NAA 0 ppm. Various concentrations of IBA and NAA did not significantly affect root formation in rubber tree shoot explants.

Morfologi Daun Durian (Durio zibethinus L.) dan Keanekaragaman Genetiknya Berdasarkan Marka ISSR Fitri Annisa; Priyanti Priyanti; Dasumiati Dasumiati
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.23975

AbstrakData keanekaragaman durian (Durio zibethinus L.) yang terbatas merupakan salah satu kendala yang dihadapi dalam pengelolaan dan pengembangannya di Indonesia. Keanekaragaman genetik dapat ditinjau berdasarkan ciri morfologi yang didukung dengan ciri molekuler. Tujuan penelitian ini adalah untuk mengetahui kekerabatan antar aksesi berdasarkan dendrogram yang menggabungkan data morfologi daun dan polimorfisme Inter Simple Sequence Repeats (ISSR). Pengamatan ciri morfologi mengacu pada deskriptor durian dan ektraksi DNA dengan metode CTAB yang telah dimodifikasi. Setelah berhasil diekstraksi, DNA durian diamplifikasi dengan tujuh primer ISSR untuk analisis polimorfisme. Konstruksi dendrogram yang terbentuk merupakan hasil analisis menggunakan program NTSYS. Berdasarkan 15 ciri morfologi yang diamati terdapat 5 ciri yang sangat membedakan antara kelompok aksesi durian. Lai (D. kutejensis) memiliki daun lebih panjang hingga 24 cm dan lebar hingga 16 cm dibandingkan 28 aksesi durian dengan panjang hingga 16 cm dan lebar hingga 9 cm. Hasil amplifikasi diperoleh sebanyak 35 pita dan 31 pita bersifat polimorfik dengan persentase polimorfisme berkisar antara 66,7–100%. Persentase polimorfisme pada penelitian ini dapat mencapai 100% dengan primer ISSR 842, PKBT 4, dan PKBT 5. Konstruksi dendrogram ciri morfologi mempunyai koefisien kemiripan sebesar 0,56–0,91 dan membentuk dua kelompok yang tidak memisahkan aksesi-aksesi durian dengan Lai. Ciri polimorfisme ISSR dapat digunakan untuk merekonstruksi dendrogram menjadi dua kelompok dengan koefisien kemiripan sebesar 0,48–0,96. Dendrogram ciri polimorfisme ISSR secara tegas memisahkan aksesi-aksesi durian dengan Lai. Pohon kekerabatan 28 aksesi durian dan Lai di Kecamatan Serpong telah direkonstruksi untuk pertama kalinya.AbstractLimited data on the diversity of durian (Durio zibethinus L.) is one of the obstacles encountered in its management and development in Indonesia. Genetic diversity can be reviewed based on morphological characteristics supported by molecular characteristics. This study aimed to determine the relationship between accessions based on a dendrogram that combines leaf morphology and Inter Simple Sequence Repeats (ISSR) polymorphism data. Observation of morphological features refered to durian descriptors and DNA extraction using the modified CTAB method. After successful extraction, durian DNA was amplified with seven ISSR primers for polymorphism analysis. The dendrogram construction is formed the NTSYS program. Based on the 15 morphological characteristics observed, 5 characteristics greatly distinguished the durian accession group. Lai (D. kutejensis) had longer up to 24 cm and wider up to 16 cm leaves than 28 durian accessions with a length (16 cm) and width (9 cm). The amplification results were obtained 35 bands and 31 out of them were polymorphic with polymorphic PCR product ranged 66.7–100%. The percentage of polymorphism could reach 100% with ISSR 842, PKBT 4, and PKBT 5 primers. The dendrogram construction based on morphological characteristics had an interaction coefficient of 0.56–0.91 and formed two groups which did not separate durian accessions with Lai. The ISSR polymorphism feature can be used to reconstruct the dendrogram into two groups with a slope coefficient of 0.48–0.96. The dendrogram based on the ISSR polymorphism feature explicitly divides durian accessions with Lai. The cluster analysis of 28 durians and Lai accessions in Serpong District has been reconstructed for the first time.

Akumulasi Logam Timbal (Pb) Pada Tanaman Bayam (Amaranthus tricolor L.) Dengan Aplikasi Pupuk Mikoriza Etyn Yunita; Dasumiati Dasumiati; Azizah Mei Widyastuti; Irzal Irda
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.35282

AbstrakPencemaran tanah oleh logam timbal (Pb) merupakan salah satu bentuk pencemaran yang sangat berbahaya bagi mahluk hidup. Salah satu tanaman bioakumulator penyerap logam berat di lingkungan adalah tanaman bayam (Amaranthus tricolor L.). Tujuan dari penelitian ini adalah untuk menganalisis pengaruh aplikasi pupuk mikoriza terhadap akumulasi Pb pada akar, batang, dan daun serta pertumbuhan tanaman bayam. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan dosis pupuk mikoriza, yaitu 0 g, 5 g, 10 g, dan 15 g per polybag. Jumlah Pb yang diaplikasikan ke media tanam adalah 5 ppm per polybag. Akumulasi Pb tertinggi terdapat pada tanaman bayam dengan perlakuan 5 g pupuk mikoriza. Rata-rata akumulasi Pb di akar, batang, dan daun pada perlakuan ini berturut-turut adalah 103,57 ppm; 36,67 ppm; dan 8,60 ppm. Pertumbuhan tanaman bayam pada perlakuan 5 g pupuk mikoriza lebih baik dari perlakuan lainnya, yaitu memiliki rata-rata tinggi tanaman dan jumlah daun tertinggi pada minggu ke-4 (9,7–12,5 cm dan 6,9–8,6 helai). Aplikasi pupuk mikoriza dapat meningkatkan akumulasi Pb pada akar, batang, dan daun serta meningkatkan pertumbuhan tanaman bayam pada dosis 5 g per polybag.AbstractSoil pollution by lead (Pb) is a form of pollution that is very dangerous for living creatures. One of the bioaccumulator plants that absorb heavy metals in the environment is spinach (Amaranthus tricolor L.). The aim of this research was to analyze the effect of mycorrhizal fertilizer application on Pb accumulation in roots, stems and leaves as well as spinach plant growth. This research used a completely randomized design (CRD) with treatment doses of mycorrhizal fertilizer, namely 0 g, 5 g, 10 g, and 15 g per polybag. The amount of Pb applied to the planting media is 5 ppm per polybag. The highest Pb accumulation was found in spinach plants treated with 5 g of mycorrhizal fertilizer. The average accumulation of Pb in roots, stems and leaves in this treatment was 103.57 ppm; 36.67 ppm; and 8.60 ppm. The growth of spinach plants in the 5 g mycorrhizal fertilizer treatment was better than the other treatments, namely having the highest average plant height and number of leaves in the 4th week (9.7-12.5 cm and 6.9-8.6 pieces). Application of mycorrhizal fertilizer can increase Pb accumulation in roots, stems and leaves and increase the growth of spinach plants at a dose of 5 g per polybag.

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