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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Bios Logos Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati INDONESIAN JOURNAL OF PHARMACY JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Annales Bogorienses
Tri Muji Ermayanti
Research Center of Biotechnology, Indonesian Intitute of Sciences (LIPI) Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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Induksi Akar Rambut Gandarusa (Justicia gendarussa Burm. f.) dengan Perlakuan Perbedaan Lama Waktu Infeksi Agrobacterium rhizogenes strain YM072001 dan A4T (The Induction of Gandarusa (Justicia gendarussa Burm. f.) Hairy Root under Different Infection Wahyuni, Dwi Kusuma; Nisa, Qonitatun; Purnobasuki, Hery; Ermayanti, Tri Muji; Prajoga, Bambang; Utami, Edy SW
JURNAL BIOS LOGOS Vol 5, No 2 (2015): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.5.2.2015.10548

Abstract

Abstrak Penelitian ini bertujuan untuk menginduksi akar rambut eksplan daun Gandarusa (Justicia gendarussa Burm.f.) dengan perlakuan perbedaan lama waktu infeksi Agrobacterium rhizogenes strain YM072001 dan A4T dan mengevaluasi lama waktu infeksi terbaik untuk induksi akar rambut. Eksplan daun diinokulasi di media MS (Murashige dan Skoog) cair yang berisi bakteri Agrobacterium rhizogenes dengan OD600 = 0,1 selama 10, 20, 30, 40, 50, dan 60 menit. Kokultivasi di MS padat selama 2 hari, lalu disubkultur ke media MS padat. Data dianalisis secara deskriptif. Akar rambut mulai tumbuh pada minggu ketiga. Induksi akar rambut berhasil pada eksplan dengan perlakuan strain YMB072001 dengan lama waktu infeksi 20, 40, dan 50 menit. Strain A4T juga berhasil menginduksi eksplan membentuk akar rambut dengan perlakuan 10 menit. Lama waktu infeksi terbaik untuk strain YMB072001 adalah 20 menit dan untuk strain A4T adalah 10 menit. Kata kunci: Agrobacterium rhizogenes, akar rambut, Justicia gendarussa Burm. f., waktu infeksi   Abstract The objective of this study were to induce hairy root of Gandarusa leaf explants by differences of Agrobacterium rhizogenes infection time treatment and to evaluate the best infection time for induction. Leaf explants were inoculated on MS (Murashige dan Skoog) liquid medium with bacterial concentrations of OD600 = 0,1 for 10, 20, 30, 40, 50, and 60 minutes and 2 days co-cultivated on MS0 solid medium then sub cultured on MS0 solid medium. Data were analyzed descriptively. Hairy roots were growing on the third week. Explants were successfully induced by strain YMB 072001 at 20, 40, and 50 minutes treatment. A4T strain was successfully induced by 10 minute of treatment. The best infection time for hairy root induction in gandarusa leaf explants for YMB 072001 strains was 20 minutes and for A4T strain was 10 minutes. Keywords: Agrobacterium rhizogenes, hairy root, infection time, Justicia gendarussa Burm. f.
Co-Authors , Juliarni . Aryanti . Harsojo Adabiyah, Rifatul Agus Purwito Al Hafiizh, Erwin Ali Husni Andi Salamah Andry, Yuli Aryanti . Aryanti Aryanti Azizah, Farroh Azizah, Farroh Bambang Prajoga Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Diah Ratnadewi Dwi Hastuti Dwi Kusuma Wahyuni Edy Setiti Wida Utami Edy SW Utami, Edy SW Efendi, Darda Hafiizh, Erwin Al Hafiizh, Erwin Al Hafiizh, Erwin Al Hamami Alfasani Dewanto Hamim Hamim Hapsari, Betalini Widhi Hapsari, Betalini Widhi Hapsari, Betalini Widhi Hermono, Arief Hermono, Arief Hery Purnobasuki Ida Ayu Putu Sri Widnyani Iman Rusmana JULIARNI JULIARNI Kartika Ika Priadi, Kartika Ika laela Sari, laela Laksono Trisnantoro Lelono, Arthur A. Lelono, Arthur A. Lizawati . Mandessy, Ary Mandessy, Ary Martin, Andri F Martin, Andri F Martin, Andri Fadillah Martin, Andri Fadillah Martin, Andri Fadillah Maulana, Evan Maulana, Evan Mukhsia, Andi Mukhsia, Andi Noorrohmah, Siti Noorrohmah, Siti Nugroho, Robertus Qonitatun Nisa, Qonitatun Rantau, Deritha E. Rantau, Deritha E. Rantau, Deritha Ellfy Rantau, Deritha Ellfy Rantau, Deritha Elly Rantau, Deritha Elly Rd. Selvy Handayani Rita Martaleta Dewi, Rita Martaleta Roedhy Poerwanto Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto Setyadi, Gesang Setyadi, Gesang Siti Nur Hafida, Siti Nur Slamet Susanto Sobir Sobir Solly Aryza Sutedja, Lenny Wiguna Rahman Wijayanta, Ardian Nur Wijayanta, Ardian Nur Wulandari, Dyah Retno Wulandari, Dyah Retno Wulansari, Aida Wulansari, Aida Yanti, Oktavia Yanti, Oktavia Yefni Syafria