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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Bios Logos Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati INDONESIAN JOURNAL OF PHARMACY JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Annales Bogorienses

Tri Muji Ermayanti

Research Center of Biotechnology, Indonesian Intitute of Sciences (LIPI) Bogor, Indonesia

Author-ID : 260507

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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Peningkatan Pertumbuhan Kultur Tunas Stevia rebaudiana Bertoni pada Media dengan Peningkatan Kadar Vitamin dan Glisin serta Penggunaan Jenis Tutup Tabung Berbeda Ermayanti, Tri Muji; Rantau, Deritha Elly; Hafiizh, Erwin Al; Maulana, Evan
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTStevia rebaudiana is plant species producing natural sweetener with has low calories. The species propagation could be done by tissue culture tecnique to obtain propagules with high quality and sustainability. Modification of media composition and in vitro environment will increase growth and vigority of explants so that they have high survival rate during acclimatization. The aim of this research was to increase growth of stevia shoot culture by increasing the level of vitamins in combination with different type of test tube covers. Stevia shoot tips were cultured on MS medium containing normal concentration of its vitamins (control treatment; Myo-inositol 100 mg/l; Nicotinic acid 0.5 mg/l; Pyridoxine-HCl 0.5 mg/l; Thiamine-HCl 0.5 mg/l and Glycine 2 mg/l), twice and 4 folds of vitamin levels, they were grown on culture tubes with Al-foil and ventilated-plastic with filter (2 cm diameter and pore size at 0,22 micron). Height of shoots, number of nodes, number of leaves, number of roots were observed every week till 8 weeks of culture. Biomass (fresh and dry weights) and chlorophyll level and acclimatization were done 8 weeks of culture. The results showed that type of culture tube covers affected significantly to all growth parameters, biomass as well as level of chlorophyll, meanwhile level of vitamins only affected number of nodes, shoots and roots. Interaction between vitamin level and covers types only occured for height of shoots and number of roots. Plantlets grown on medium containing 4 fold of vitamin level (Myo-inositol 400 mg/l; Nicotinic acid 2 mg/l; Pyridoxine-HCl 2 mg/l; Thiamine-HCl 2 mg/l and Glycine 8 mg/l) with ventilated-plastic cover had larger leaves compared to other treatments. All plantlets survived in a greenhouse.Keywords: Stevia rebaudiana, in vitro growth, increase in vitamin concentration.

Induksi Tanaman Poliploid Talas (Colocasia esculenta L.) dengan Perlakuan Orizalin secara In Vitro Wulansari, Aida; Martin, Andri F; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTGenetic improvement of taro (Colocasia esculenta L.) is one of important research program to increase productivity and give better cultivation on the marginal land. Induction of polyploid plants is a method useful to increase genetic diversity. The aim of this research was to induce polyploid taro by oryzalin treatment. Polyploidy was induced from in vitro diploid taro âbentulâ using oryzalin at 7.5; 15; 30; 60 and 75 ?M soaked for 3 days. Regenerated shoots were grown on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine. Shoot growth was recorded four weeks (subculture-0), eight weeks (subculture-1) and twelve weeks (subculture-2) after treatments. The results showed that survival rate of treated shoots was 100%. Higher oryzalin concentration reduced the proliferation of shoots, petiole length, numbers of leaves as well as the numbers of roots. Ploidy levels analysis determined by flowcytometer for 122 plantlets were investigated. The results indicated that control shoots were diploid. All treated shoots were polyploids. Oryzalin at 60 ?M gave 50% of tetraploid planlets, 30 ?M of oryzalin gave 5.71% hexaploids, 60 ?M of oryzalin gave 9.09% octaploids. All acclimatized plantlets gave 100% survival rate.Keywords : Colocasia esculenta L., taro, in vitro induced polyploidy, oryzalin

Acclimation and Agronomic Performance of Polyploids Clones of Artemisia annua L. Rahman, Wiguna; Hafiizh, Erwin Al; Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Lelono, Arthur A.
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3092

ABSTRACTSomatic cell manipulation of Artemisia annua L. was conducted by induction of polyploid plants with Colchicine and Oryzalin in order to increase level of artemisinin. Polyploid plantlets were multiplied on MS medium without plant growth regulators. After acclimation processes, plants were grown in the field for agronomic performance observation. Survival rate of plantlets was recorded. Agronomic performance of plants was observed by recording height of plants, number of branches, leaf biomass, stomatal characteristics, and artemisinin content. The results showed that survival rate of the plantlets from Colchicine and Oryzalin treatments were ranging from 13.40 to 33.33% and 11.11 to 41.67%, respectively. Growth rates of plant height and plant branching were not significantly different between diploid and tetraploid plant both from Colchicine and Oryzalin treatments, except to triploid plants from Colchicine treatment. Averages of plant height from Colchicine and Oryzalin treatments were ranging from 10.0 to 220.0 cm and from 35.0 to 186.0 cm, respectively. The averages number of branches per plant of polyploid plants from Colchicine and Oryzalin treatments were ranging from 3 to 66 and from 11 to 63, respectively. Averages of dry leaves biomass between diploid and tetraploid plant from Colchicine and Oryzalin treatments were also not significantly different. They were ranging from 12 to 64 g/plant and from 11 to 62 g/plant, respectively. However, tetraploid clones have bigger size of stomata and produced more artemisinin than the diploids.Keywords: Artemisia annua L, Colchicine, Oryzalin, Polyploids, Acclimation, Agronomic performance

Klaster Pertumbuhan Kultur Tunas Talas (Colocasia esculenta (L.) Schot.) cv. Bentul Tetraploid Berdasarkan Metode Ward Wulansari, Aida; Martin, Andri Fadillah; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTTaro (Colocasia esculenta (L.) Schot.) has been cultivated for a long time in Indonesia. Taro tuber can be used as alternative food for rice substitute to support food diversification program. Taro cv. Bentul is preferable to farmers because it has few buds that are easy to maintain and adaptable in both lowlands and highlands. Its tuber has a delicious taste and a soft texture. Somatic cell manipulation of Indonesian taro through biotechnology may contribute to increase its productivity. One of in vitro technique for somatic cell manipulation is polyploidy. Oryzalin has been able to obtain taro cv. Bentul tetraploid. A total of 17 tetraploid clones were used in this study for clustering. Those clones were obtained from previous research and have stable ploidy level. The objective of this study was to determine cluster Bentul tetraploid of shoot culture using Ward method based on their in vitro growth as an initial selection before further selection in the field. Shoot tips of tetraploid were cultured on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine for 6 weeks. The observed growth variables were number of shoots, length of petiole, number of leaves and roots. The clusterring was done using Ward and Euclidean Distance method followed by Analysis of Varians and Duncan Multiple Range Tests (DMRT). Out of 17 clones observed resulted in 3 clusters. Cluster 1 consisted of 2 clones, cluster 2 consisted of 9 clones, and cluster 3 consisted of 6 clones respectively. The best cluster was cluster 3 which was significantly different on the average number of shoots and leaves. Cluster 3 was dominated by clones derived from oryzalin at 75 ?M.Keywords: taro (Colocasia esculenta (L.) Schot.), tetraploid, in vitro growth, cluster analysis, Ward

Variasi Jumlah Kromosom Planlet Taraxacum officinale Weber ex FH. Wigg Hasil Regenerasi in vitro dari Eksplan Akar, Helai Daun dan Tangkai Daun Ermayanti, Tri Muji; Lestari, Indah; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Taraxacum officinale Weber ex F. H. Wigg. is a herbaceous medicinal plant species belonging family Asteraceasewhich has apomictic and poliploid characteristics. Multiplication of shoots using tissue culture was used to obtaindefine high quality seedlings, uniform, stable or free of diseases. However, changes in chromosome number canoccur in regenerated plants. The research aim was to determine the chromosome number of T. officinale plants regeneratedfrom culture in vitro using explants of roots, petioles and leaf blades. Therefore, selection of regenerantscan be done in order to find out transplants which have high yield of secondary metabolites. Analysis of chromosomenumber from root tips samples was conducted using 24 plantlets regenerated from root, 27 plantlets regeneratedfrom leaf blade, 21 plants regenerated from petiole and 102 roots of grown seeds using orcein squash method.The results showed that germinating seeds (control) and regenerated plants had variation in chromosome number.The range of chromosome numbers from regenerated plants were 2n=8-39, and cells with diploid number (2n = 2x= 16) was as most observed. The range numbers in germinated seeds were 2n=10-38, and cells with triploid number(2n = 3x = 24) was as most observed. This results proved that variation in numbers of chromosome was caused byapomixis and poliploid characteristics of the parent plant regenerated to their regenerants.Keywords : Taraxacum officinale. Weber ex F. H. Wigg, in vitro regeneration, variasi, chromosome

Induksi Poliploidi pada Tanaman Talas (Colocasia esculenta (L.) Schott) Kultivar Kaliurang dengan Perlakuan Kolkisin secara In Vitro Ermayanti, Tri Muji; Wijayanta, Ardian Nur; Ratnadewi, Diah
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTGenetic modification to increase productivity and other better growth characteristics of Kaliurang taro could be done by various methods; one of them is polyploid induction. Kaliurang taro has performed excellent traits, it is resistant to leaf blight disease and pests. Polyploid plants of Kaliurang taro are expected to have excellent char-acteristics and increased productivity. The objective to this study was to obtain an efficient method through in vitro induction of polyploidy using colchicine on Kaliurang taro. Aseptic plantlets of Kaliurang taro were soaked in colchicine solution at 0.05, 0.1 and 0.2% for 1, 2 and 3 days. Control was untreated plantlets. Each treatment consisted of 12 replicates. The colchicine-treated plantlets were then planted on MS medium containing 2 mg/L BAP, 1 mg/L thiamine and 2 mg/L adenine. Survival rate and vegetative growth of plantlets were observed every week for 8 weeks after planting. The results showed that increasing colchicine concentra-tion and the soaking period produced planlets with various survival rate. Flowcytometric analysis indicated that the treatment of soaking the plantlets in 0.05% colchicine for 1 day resulted in 14.3% of tetraploid plantlets. The most efficient of colchicine treatment was 0.2% for 3 days, resulting in 57.1% tetraploids, with the efficient value of 33.3%. There was chromosome multiplication from diploid to tetraploid which was confirmed through both flowcytometric analysis and chromosomes counting.Keywords: Taro (Colocasia esculenta L.), Kaliurang, in vitro, flowcytometer, chromosome multiplication, tetraploids

Pengaruh Modifikasi KH2PO4, NH4NO3 dan Sukrosa terhadap Pertumbuhan Tunas serta Pembentukan Umbi Mikro Taka (Tacca leontopetaloides) secara In vitro Rudiyanto, Rudiyanto; Hapsari, Betalini Widhi; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3658

ABSTRACTPolynesian arrowroot (Tacca leontopetaloides (L.) Kuntze), which is one of the bulbous herbaceous plants,have high nutritional value. Modification of macro nutrients by reducing nitrogen content and increasingphosphorus on the medium gave affects on shoot growth and initiated micro tuber formation on in vitrocultures. The aim of this research was to determine the effect of modified macro nutrients in combination withthe increase in sucrose concentrations on shoot growth and micro tuber formation of T. leontopetaloides. Theexperimental design was factorial completely randomized design.The factors tested were modifications of MSmacro nutrients that were. M1 (170 mg/l KH2PO4 and 1650 mg/l NH4NO3; normal, control treatment); M2 (340mg/l KH2PO4 and 825 mg/l NH4NO3); and M3 (680 mg/l KH2PO4 and 412.5 mg/l NH4NO3 in combinationwith 30 (S1) (control treatment), 40 (S2), 50 (S3) and 60 g/l of sucrose (S4). The variables tested were shootheight, number of leaves, number of roots and number of micro tuber which were observed weekly at 0-8weeks after culturing. The results showed that the modification of macro nutrient in combination with sucroseconcentration had significant effect on shoot height, number of leaves and number of roots but not significanton the number of tubers. The highest shoots were found in M1S3 treatment, the highest number of leaves wasin M1S1 and M1S3 treatment and the highest number of roots was in M1S4 treatment. The number of tubersnot significantly different between the treatments tested.Keywords: in vitro, KH2PO4, microtuber, NH4NO3, sucrose, Tacca leontopetaloides

EVALUASI PERTUMBUHAN STEVIA REBAUDIANA BERT. TETRAPLOID SECARA IN VITRO DAN DI LAPANG UNTUK PRODUKSI STEVIOSIDA DAN REBAUDIOSIDA-A Adabiyah, Rifatul; Ratnadewi, Diah; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3809

ABSTRACTGenetic improvement through tetraploid induction of Stevia rebaudiana is important in order to increase the sweetener content, steviol glycoside. Tetraploid plants of several species after induction with colchicine and oryzalin have higher growth and secondary metabolite contents compared to the diploid plants. This study was aimed to evaluate growth as well as their stevioside and Reb-A content of S. rebaudiana tetraploid and diploid (control) plants cultured in vitro and grown in the field after acclimation process. This study used 3 tetraploid clones, namely B60.3H8, P1T22, P3T5, and 1 diploid clone as control. Shoot tips were cultured on MS medium without addition of plant growth regulators for 6 weeks, then they were acclimated in a greenhouse, followed by planting them in the field. Growth of shoot culture, plantlets in the greenhouse and plants in the field were observed. Analysis of stevioside and Reb-A was done by HPLC. The results showed that plantlets of diploid clone had higher in vitro growth and survival rate in the greenhouse than that of tetraploids. Tetraploid clone P1T22 had similar growth as diploid plants, but higher than the growth of tetraploid B60.3H8 and P3T5. Fresh and dry weights of B60.3H8 was similar with diploid plants, but higher than P1T22 and P3T5 tetraploid clones. The highest level of stevioside and Rebaudiosida-A was found in tetraploid B60.3H8 clone, the lowest was found in the diploid plants. The highest ratio of stevioside : Reb-A was found at B60.3H8 tetraploid clone. Keywords: Stevia rebaudiana, in vitro, field, growth, Stevioside, Rebaudioside-A, tetraploid

VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers. Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro

INDUKSI TANAMAN POLIPLOID TALAS (COLOCASIA ESCULENTA L.) DENGAN PERLAKUAN ORIZALIN SECARA IN VITRO Wulansari, Aida; Martin, Andri F; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2898

ABSTRACTGenetic improvement of taro (Colocasia esculenta L.) is one of important research program to increase productivity and give better cultivation on the marginal land. Induction of polyploid plants is a method useful to increase genetic diversity. The aim of this research was to induce polyploid taro by oryzalin treatment. Polyploidy was induced from in vitro diploid taro ?bentul? using oryzalin at 7.5; 15; 30; 60 and 75 ?M soaked for 3 days. Regenerated shoots were grown on MS medium containing 2 mg/l BAP, 1 mg/l thiamine and 2 mg/l adenine. Shoot growth was recorded four weeks (subculture-0), eight weeks (subculture-1) and twelve weeks (subculture-2) after treatments. The results showed that survival rate of treated shoots was 100%. Higher oryzalin concentration reduced the proliferation of shoots, petiole length, numbers of leaves as well as the numbers of roots. Ploidy levels analysis determined by flowcytometer for 122 plantlets were investigated. The results indicated that control shoots were diploid. All treated shoots were polyploids. Oryzalin at 60 ?M gave 50% of tetraploid planlets, 30 ?M of oryzalin gave 5.71% hexaploids, 60 ?M of oryzalin gave 9.09% octaploids. All acclimatized plantlets gave 100% survival rate.Keywords : Colocasia esculenta L., taro, in vitro induced polyploidy, oryzalin

Co-Authors , Juliarni . Aryanti . Harsojo Adabiyah, Rifatul Agus Purwito Al Hafiizh, Erwin Ali Husni Andi Salamah Andry, Yuli Aryanti . Aryanti Aryanti Azizah, Farroh Azizah, Farroh Bambang Prajoga Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Diah Ratnadewi Dwi Hastuti Dwi Kusuma Wahyuni Edy Setiti Wida Utami Edy SW Utami, Edy SW Efendi, Darda Hafiizh, Erwin Al Hafiizh, Erwin Al Hafiizh, Erwin Al Hamami Alfasani Dewanto Hamim Hamim Hapsari, Betalini Widhi Hapsari, Betalini Widhi Hapsari, Betalini Widhi Hermono, Arief Hermono, Arief Hery Purnobasuki Ida Ayu Putu Sri Widnyani Iman Rusmana JULIARNI JULIARNI Kartika Ika Priadi, Kartika Ika laela Sari, laela Laksono Trisnantoro Lelono, Arthur A. Lelono, Arthur A. Lizawati . Mandessy, Ary Mandessy, Ary Martin, Andri F Martin, Andri F Martin, Andri Fadillah Martin, Andri Fadillah Martin, Andri Fadillah Maulana, Evan Maulana, Evan Mukhsia, Andi Mukhsia, Andi Noorrohmah, Siti Noorrohmah, Siti Nugroho, Robertus Qonitatun Nisa, Qonitatun Rantau, Deritha E. Rantau, Deritha E. Rantau, Deritha Ellfy Rantau, Deritha Ellfy Rantau, Deritha Elly Rantau, Deritha Elly Rd. Selvy Handayani Rita Martaleta Dewi, Rita Martaleta Roedhy Poerwanto Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto Setyadi, Gesang Setyadi, Gesang Siti Nur Hafida, Siti Nur Slamet Susanto Sobir Sobir Solly Aryza Sutedja, Lenny Wiguna Rahman Wijayanta, Ardian Nur Wijayanta, Ardian Nur Wulandari, Dyah Retno Wulandari, Dyah Retno Wulansari, Aida Wulansari, Aida Yanti, Oktavia Yanti, Oktavia Yefni Syafria

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