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Antibacterial Potency of Pleurotus ostreatus Extract From Fruiting Body and its Solid Substrate on Staphyllococcus aureus I. Badarina; D. Evvyernie; E. N. Herliyana; L. K. Darusman; T. Toharmat
Jurnal Sain Peternakan Indonesia Vol 15, No 2 (2020)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.15.2.142-147

Abstract

The objective of this research was to evaluate the antibacterial potency of ethanol extract from fruiting body of  Pleurotus ostreatus and Its solid substrate made from coffee husk and sawdust on the growth of Staphylococcus aureus ATCC 25922.  The extract of Ganoderma lucidum fruiting body and Tetracycline antibiotic paper disk 30µg/disk were used as control.  The samples were extracted by using maceration method in  30% ethanol solution.  The extracts were diluted with sterile distilled water to concentration 500, 1000, and 5000ppm.  The result showed that the ethanol extracts from fruiting body  of  Pleurotus ostreatus and Ganoderma lucidum and the extracts of coffee husk and sawdust substrate fermented by Pleurotus ostreatus could inhibit the growth of bacteria for all the concentration. There was a significant difference in diameter of cleared zones between  Tetracycline antibiotic disc 30?g  and the ethanol extracts of the samples (p<0.01).  The diameter of cleared zones among the sample extracts and each dilution concentrations were not a significant difference (p>0.05).  Tetracycline was sensitive to S.aureus ATCC25922, while all the extracts were resistant.   This study confirmed that there were the antibacterial potency of mushroom extracts from the fruiting body and also its solid substrates.
Evaluation of Mineral Contents in Milk of Dairy Cattle Fed Elephant Grass Planted at Ex-Coal Mining Land H. Harmini; D. Evvyernie; P. D. M. H. Karti; Y. Widiawati
Tropical Animal Science Journal Vol. 43 No. 4 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.4.322

Abstract

The growth of several types of grass in the area of ​​mine re-vegetation is rapidly providing an opportunity to be used as forages despite the possibility of heavy metal contamination. The purpose of the study was to evaluate the effect of treatment of different levels of elephant grass (EG) (Pennisetum purpureum) planted at ex-coal mining reclamation on milk productivity and mineral contents of dairy cattle. This study used four lactating (second lactation) dairy cattle using Latin Square Design of 4 (treatments) x 4 (replications). Each period was 21 days consisted of 14 days of preliminary for feed adaptation and 7 days for observation. Treatments were P0 (0% ex-coal mining (EEG)+60% Farmer’s EG (FEG)+40% Concentrate), P1 (15% EEG+45% FEG+40% Concentrate), P2 (30% EEG+30% FEG+40% Concentrate), and P3 (60% EEG+0% FEG+40% Concentrate). The study observed variables of feed intake and efficiencies, milk production, milk quality, and mineral contents of milk. Results showed that EEG treatment at different levels did not affect fresh and dry matter intake. The highest fresh and dry matter intake was found in P0 treatment. There was no significant difference in giving EG planted at the ex-coal mining and the farmer’s land on the milk production and milk quality. The only significant differences (p<0.05) were found in mineral Fe and Mg contents of milk. The study concluded that giving EG planted at the ex-coal mining area until 60% of forage affected Fe and Mg contents of milk, but they are still in the safe limit.
PERFORMA SAPI POTONG SEBAGAI RESPON DARI SUPLEMENTASI PROBIOTIK PADAT DAN CAIR Dian Purwanti; Suryahadi Suryahadi; Dwierra Evvyernie Amirroenas
Buletin Ilmu Makanan Ternak Vol. 12 No. 1 (2014): BULETIN MAKANAN TERNAK
Publisher : Buletin Ilmu Makanan Ternak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (860.361 KB)

Abstract

(PERFORMANCE OF BEEF CATTLE IN RESPONSE TO SOLID AND LIQUID PROBIOTIC SUPPLEMENTATION)Probiotics are live microbial feed supplements form, providing beneficial effects for the host animal by improving the balance of microorganisms in the digestive tract. This study aims to examine the benefits of probiotic supplementation in the form of liquid and solid on performance of beef cattle. The treatment used is as follows: P1: Ration Control (forage + concentrate + rice bran), P2: Ration Control + 0.25% solids probiotic in the concentrate, P3: Ration Control + 1% liquid probiotic through drinking water. Experimental design used was Randomized Block Design with 18 cows that are divided into six groups based on body weight and 3 treatment ration. Parameters measured were consumption of nutrients, body weight gain, efficiency of feed utilization, and Income Over Feed Costs (IOFC). The collected data were analyzed by analysis of variance. The test results statistically showed that the treatment was not significant (P> 0.05) effect on the consumption of nutrients, body weight gain and efficiency of feed utilization. Keywords: beef cattle, liquid probiotics, performance, solid probiotics
Pengaruh Kandungan Protein Ransum yang Berbeda terhadap Kecernaan dan Fermentabilitas Rumen Sapi Perah secara In vitro: The Effect of Different Feed Protein Content on Digestibility and Fermentability of Dairy Rumen using In vitro Rika Zahera; Dian Anggraeni; Zikri Aulia Rahman; Dwierra Evvyernie
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 18 No. 1 (2020): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1057.477 KB) | DOI: 10.29244/jintp.18.1.1-6

Abstract

The most of protein requirement for cows is fulfilled by microbial protein. Increasing the protein from ration usually influences the milk production in dairy cows. However, the availability of dietary protein should support rumen ecosystem through microbial activity in the fermentation and digestion process. The aim of this study was to evaluate the protein levels of dairy cow ration on the rumen fermentability and digestibility using an in vitro method. Randomized block design with three levels of protein ration as a treatment and three times taken of rumen liquor as a block. The treatments were: R1= ration with low protein; R2= ration with moderate protein and R3= ration with high protein. The measured parameters were rumen fermentability (total VFA, N-NH3 and pH), in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD). The results showed that there were significantly increase in dry and organic matter digestibility (p<0.05), due to increasing the level of dietary protein, but there was no effect in the fermentability, except a slight increase in N-NH3 concentration. The conclusion of this study was the highest level of protein ration enhances the digestibility, and showed a tendency for higher N-NH3. Key words: dairy cattle, dietary protein, digestibility, fermentability, in vitro DAFTAR PUSTAKA [Ditjennak-Keswan] Direktorat Jendral Peternakan dan Kesehatan Hewan. 2019. Pemerintah dorong perbaikan kualitas dan kuantitas susu nasional [internet]. Tersedia pada: http://ditjennak.pertanian.go.id/pemerintah-dorong-perbaikan-kualitas-dan-kuantitas-susu-nasional Anggraeny YN, Soetanto H, Kusmartono & Hartutik. 2015. Sinkronisasi suplai protein dan energi dalam rumen untuk meningkatkan efisiensi pakan berkualitas rendah. WARTAZOA. 25(3):107–116 Chanthakhoun V, Wanapat M & Berg J. 2012. Level of crude protein in concentrate supplements influenced rumen characteristics, microbial protein synthesis and digestibility in swamp buffaloes (Bubalus bubalis). Livestock Science. 144(3):197–204. Cherdthong A & Wanapat M. 2013. Manipulation of in vitro ruminal fermentation and digestibility by dried rumen digesta. Livestock Science. 153(1–3):94–100. Colmenero JJO & Broderick GA. 2006. Effect of dietary crude protein concentration on milk production and nitrogen utilization in lactating dairy cows. Journal of Dairy Science. 89(5):1704–1712. Conway E. 1957. Microdiffusion of Analysis of Association Official Analytical Chemist. Georgia (US): Georgia Press. Despal, Permana IG, Safarina SN & Tatra AJ. 2011. Penggunaan berbagai sumber karbohidrat terlarut air untuk meningkatkan kualitas silase daun rami. Media Peternakan. 34(2):69–76. Despal, Zahera R, Lestari DA, Ma’rifah H & Permana IG. 2015. Ketersediaan dan kualitas sumberdaya pakan musim kemarau dan dampaknya terhadap pemenuhan nutrien dan performa sapi perah di Pangalengan Kabupaten Bandung. Sumedang (ID): Seminar Nasional Peternakan Berkelanjutan, Universitas Pajajaran Dung DV, Shang W & Yao W. 2014. Effect of crude protein levels in concentrate and concentrate levels in diet on in vitro fermentation. Asian-Australasian Journal of Animal Science. 27(6):797–805. Forejtová J, Lád F, Třináctý J, Richter M, Gruber M, Doležal P, Homolka P & Pavelek L. 2005. Comparison of organic matter digestibility determined by in vivo and in vitro methods. Czeh Journal of Animal Science. 50(2):47–53. Hernaman I, Tarmidi AR & Dhalika T. 2017. Kecernaan in vitro ransum sapi perah berbasis jerami padi yang mengandung konsentrat yang difermentasi oleh Saccharomyces cerevisiae dan Effective Microorganisms-4 (EM-4). Majalah Ilmu Peternakan. 20(2):45–48. Holik YLA, Abdullah L & Karti PDMH. 2019. Evaluasi nutrisi silase kultivar baru tanaman sorgum (Sorghum bicolor) dengan penambahan legum Indigofera sp. pada taraf berbeda. Jurnal Ilmu Nutrisi dan Teknologi Pakan. 17(2):38–46. Imran M, Pasha TN, Shahid MQ, Babar I & Naveed M. 2017. Effect of increasing dietary metabolizable protein on nitrogen efficiency in Holstein dairy cows. Asian-Australasian Journal of Animal Science. 30(5):660–665. Joo JW, Bae GS, Min WK, Choi HS, Maeng WJ, Chung YH & Chang MB. 2005. Effect of protein sources on rumen microbial protein synthesis using rumen simulated continuous culture system. Asian-Australasian Journal of Animal Science. 18(3):326–331. Law RA, Young FJ, Patterson DC, Kilpatrick DJ, Wylie ARG & Mayne CS. 2009. Effect of dietary protein content on animal production and blood metabolites of dairy cows during lactation. Journal of Dairy Science. 92(3):1001–1012. Leonardi C, Stevenson M & Armentano LE. 2003. Effect of two levels of crude protein and methionine supplementation on performance of dairy cows. Journal of Dairy Science. 86(12):4033–4042. Lestari DA, Abdullah L & Despal. 2015. Comparative study of milk production and feed efficiency based on farmer best practices and National Research Council. Media Peternakan. 38(2): 110-117 McDonald P, Edwards R, Greenhalgh J, Morgan C, Sinclair L & Wilkinson R. 2010. Animal Nutrition. Seventh Ed. London (UK): Pearson Education McMurphy C, Duff G, Sanders S, Cuneo S & Chirase N. 2011. Effects of supplementing humates on rumen fermentation in Holstein steers. South Asian-Australasian Journal of Animal Science. 41(2):134–140. Nadeau E, Englund J & Gustafsson AH. 2007. Nitrogen efficiency of dairy cows as affected by diet and milk yield. Livestock Science. 111(1–2):45–56. NRC. 1978. Nutrient Requirements of Dairy Cattle. 5th Revised Ed. Washington (US): National Academy Press. NRC. 2001. Nutrient Requirement of Dairy Cattle. 7th Revised Ed Washington (US): National Academy Press Permana IG, Despal, Zahera R & Damayanti E. 2017. Evaluasi kecukupan nutrien, produksi dan kualitas susu sapi perah di peternakan rakyat. Bogor (ID): Seminar Nasional Industri Peternakan, Fakultas Peternakan IPB. Saha S, Gallo L, Bittante G, Schiavon S, Bergamaschi M, Gianesella M & Fiore E. 2019. Rumination time and yield, composition, lactating holstein cows. Animals. 9(2):1–13. Shahzad MA, Tauqir NA, Ahmad F, Nisa MU, Sarwar M & Tipu MA. 2011. Effects of feeding different dietary protein and energy levels on the performance of 12 – 15-month-old buffalo calves. Tropical Animal Health Production. 43(3):685–694. Sucak MG, Serbester U & Görgülü M. 2017. Effects of dietary starch and crude protein levels on milk production and composition of dairy cows fed high concentrate diet. Turkish Journal of Agriculture - Food Science and Technology. 5(6):563–567. Suharti S, Aliyah DN & Suryahadi. 2018. Karakteristik fermentasi rumen in vitro dengan penambahan sabun kalsium minyak nabati pada buffer yang berbeda. Jurnal Ilmu Nutrisi dan Teknologi Pakan. 16(3):56–64. Xia C, Aziz M, Rahman U, Yang H, Shao T, Qiu Q, Su H & Cao B. 2018. Effect of increased dietary crude protein levels on production performance, nitrogen utilization, blood metabolites and ruminal fermentation of Holstein bulls. Asian-Australasian Journal of Animal Science. 31(10):1643–1653. Zahera R, Permana IG & Despal. 2015. Utilization of mungbean’s greenhouse fodder and silage in the ration for lactating dairy cows. Media Peternakan. 38(2):123–131
Impact of Inoculant Sources on Feed in Vitro Fermentability and Digestibility : Dampak Cairan Rumen Sumber Inokulan pada Fermentabilitas dan Kecernaan Pakan secara In Vitro A K Agustina A K Agustina; D Evvyernie; Rika Zahera; I G Permana; Toto Toharmat; S Suryahadi; D Despal
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 18 No. 3 (2020): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.18.3.89-94

Abstract

The aim of this research is to compare alternative inoculant source for in vitro rumen fermentation. In the first experiment, inoculant from fistulated cattle kept in LIPI and IPB (Fis1 and Fis2) and inoculant from Bogor municipality abattoir and IPB abattoir (Abo1 and Abo2) were tested for their pH, total bacterial count, and protozoal number using a complete block design with four replications. In the second experiment, the effect of the inoculant sources was tested on cornmeal (F1), soybean oil meal (F2), Napier grass (F3), and dairy cattle complete ration (F4) fermentability and digestibility including pH, VFA, NH3, IVDMD and IVOMD parameters. The results showed an unsignificant different protozoal number among inoculant sources. The pH of Fis2 rumen liquor was significantly lower (p<0.05) than others. The bacterial population was significantly higher (p<0.05) in Fis2 and Abo2 than Abo1, and Fis1. The inoculant pH after feed fermentability was not influenced by feed type but inoculant source with Fis1 was significantly higher (p<0.05) than Fis2, Abo2, and Abo1. The ammonia, VFA concentration, IVDMD, and IVOMD were influenced by interaction between inoculant sources and feed types. Although inoculant from cattle close to the laboratory (Fis2 and Abo2) were better in term of higher bacterial population, higher fermentability and digestibility for most type of feeds but other sources can be used in vitro study without differences in average fermentability and digestibility results. Key words: abattoir, fermentability, fistula, inoculant, in vitro
Isolasi dan karakterisasi bakteri yang berpotensi mengikat aflatoksin di rumen sapi Dwi Sisriyenni; Suryahadi Suryahadi; Komang G Wiryawan; Dwierra Evvyernie; Dadik Pantaya
Jurnal Ilmu Peternakan Terapan Vol 4 No 2 (2021): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study was to examine the influence of aflatoxins on rumen fermentation in vitro and obtained rumen bacterial isolates capable of binding aflatoxin. This trial consisted of three stages. The first trial was a reduction of aflatoxin in the rumen in vitro. The second experiment was the isolation and characterization of rumen bacteria that could bind aflatoxin. The third stage was to test the holding capacity of aflatoxin by rumen bacteria. This study used cow rumens. The Research design used a randomized complete block design (RCBD) factorial 2x3 and 2 replications. The first factor was the presence of aflatoxin (with and without the addition of aflatoxin), the second factor was the type of feed (diet and glucose), and the third factor was the time of incubation (0 and 4 hours). The results showed the presence of aflatoxin did not affect rumen conditions (pH, volatile fatty acid (VFA), and rumen lactic acid concentration), and the results obtained 6 isolates and isolation of rumen bacteria to bind aflatoxin up to 50%.
Populasi Mikroba Rumen, Fermentabilitas, dan Kecernaan Suplementasi Daun Kelor dalam Ransum Sapi Perah secara In Vitro: Microbial Rumen Population, Fermentability, and Digestibility of Moringa Leaf Supplementation in Dairy Cow Ration using In Vitro Rika Zahera; Junita Purwanti; Dwierra Evvyernie
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 3 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.3.117-122

Abstract

This study aimed to evaluate the microbial rumen population, fermentability, and digestibility of Moringa leaf supplementation in dairy cow ration using in vitro and to determine the optimal level of supplementation. The experiment consist of two steps with the first step was microbiology measurement used a Randomized Block Design with 5 treatments level of Moringa leaf extract (P0= control; P1= 5%, P2 = 10%, P3 =15%, P4 =20%) and the second step was in vitro fermentability and digestibility measurement used Randomized Block Design with 7 treatments level of Moringa leaf in dairy cow ration (R0 = control, R1 = R0 + 2.5% Moringa leaf, R2= R0 +5% Moringa leaf, R3 = R0 + 7.5% Moringa leaf, R4= R0+10% Moringa leaf, R5=R0+12.5% Moringa leaf, R6=R0+15% Moringa leaf) which grouped by rumen fluids. Data analysis used analysis of variance and continued with Duncan’s Multiple Range Test. The measured variable were microbial rumen population (bacteria and protozoa), fermentability (N-NH3, VFA), microbial protein synthesis, dry matter digestibility (DMD), and organic matter digestibility (DMO). The results showed Moringa leaf extract significantly decreased bacterial population (p<0.05), but there was no effect on the protozoa population. Moringa leaf supplementation did not affect N-NH3, DMD, and DMO, but significantly influenced VFA concentration and microbial protein synthesis (p<0.01). The higher Moringa leaf supplementation showed decreasing total VFA concentration, but was still within the normal range for rumen fermentation (102.29-126.69 mM). Moringa leaf supplementation showed a quadratic effect on microbial protein synthesis with an optimal supplementation level of 5%, but decreasing at a level of 7.5% still within in normal range. It can be concluded Moringa leaf can be supplemented up to 7.5% in dairy cow ration. Key words: digestibility, fermentability, in vitro, moringa leaf, dairy cow
Effect of betel leaf meal (Piper Betle L.) as feed additive on milk composition and somatic cell count of subclinical mastitis Abdul Alim Yamin; Asep Sudarman; Dwierra Evvyernie
Jurnal Ilmu Ternak Vol 22, No 2 (2022)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v22i2.40525

Abstract

The aim of this experiment was to study the effect of betel leaf meal (BLM) as feed additive on milk composition and somatic cell count of subclinical mastitis infected cow. The experiment design was a completely randomized design with five dietary treatments of BLM level and four replications in concentrate feed. The treatments were T0 (without betel leaf meal, as control), T1 (2% of betel leaf meal every day), T2 (4% of betel leaf meal every day), T3 (2% of betel leaf meal skip two days), and T4 (4% of betel leaf meal skip two days). Milk samples from the infected quarter would be analyzed every week of the experiment. Parameters measured were milk composition and somatic cell count. Data were analyzed by using analysis of variance (ANOVA) and the differences among treatments were examined by Duncan's multiple range test. Results showed that in T1 decreased somatic cell count by 83%, but the addition of BLM did not significantly affect milk composition. It could be concluded that the addition of 2% of BLM as a feed additive has the potency to prevent mastitis in lactating cows.
Fermentation Characteristics (In Vitro) of Palm Oil Trunk Waste as Feed for Lactating Dairy Cow: Kajian in vitro limbah batang kelapa sawit sebagai pakan ternak sapi perah Shilvia, S; Permana, Idat Galih; Evvyernie, D; R, A
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 21 No. 3 (2023): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.21.3.150-155

Abstract

This study aimed to evaluate the applicability of palm oil trunk waste utilization as a feed source for lactating dairy cows using an in vitro approach. This study used a randomized complete block design with 4 treatments and 3 groups. Treatment consisted of P0 (control diet), P1 (control diet supplemented with 12.5% palm oil trunk), P2 (control diet supplemented with 25% palm oil trunk), P3 (control diet with commercial concentrate). The parameters observed were rumen pH, NH3 concentration, total VFA concentration, and in vitro dry matter and organic matter digestibility (IVDMD and IVOMD) coefficients. The findings of this study showed a significant 41.73% in NH3 concentration, 24.96% in total VFA concentration, 10.47% in IVDMD, and 10.91% in IVOMD, upon introducing 25% palm oil trunk waste into the ration (p<0.05), except for rumen pH. It can be concluded that palm oil trunk waste can be used at a level of up to 25% in the diet of lactating dairy cows. Keywords: digestibility, fermentation, in vitro, palm oil trunk, unconventional feed
Studi In Vitro Temulawak (Curcuma xanthorrhiza) dan Kencur (Kaempferia galanga) sebagai Feed Additive Alami Terhadap Fermentabilitas dan Protozoa Rumen: Study of Fermentability and Rumen Protozoa from Javanese Turmeric (Curcuma xanthorrhiza) and Aromatic Ginger (Kaempferia galanga) as Natural Feed Additives A Rosmalia; Evvyernie, Dwierra; D E Yolanda; D M Fassah
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 3 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.3.173-177

Abstract

This research investigated the potential of Javanese turmeric (Curcuma zanthorrhiza) and aromatic ginger (Kaempferia galanga) as natural feed additives in dairy cow diets, focusing on their effects on rumen fermentation characteristics and and protozoa population through an in vitro study. The study employed a randomized block design consisting of four treatments and three replicates, utilizing rumen fluid obtained from three fistulated Friesian Holstein bull dairy cows. The treatments were defined as follows: P0 = control diet; P1 = P0 + 10% Javanese turmeric; P2 = P0 + 10% aromatic ginger; P3 = P0 + 5% Javanese turmeric + 5% aromatic ginger. The parameters assessed included rumen pH, ammonia (NH3) concentration, total volatile fatty acids (VFA) concentration, and protozoa population. Data analysis was performed using analysis of variance (ANOVA), followed by Duncan’s multiple range test to identify significant differences (p<0.05). The results showed that none of the treatments significantly affected on the measured parameters (rumen pH, ammonia, VFA, and protozoa populations). The best results were observed in treatment P2 (10% aromatic ginger), which exhibited average values within the normal range across all parameters. The study concluded that dietary inclusion of Javanese turmeric and aromatic ginger, either alone or combined, did not disrupt rumen fermentation balance.