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Nutrient Digestibility and Performances of Frisian Holstein Calves Fed with Pennisetum purpureum and Inoculated with Buffalo’s Rumen Bacteria I Prihantoro; D Evvyernie; . Suryani; L Abdullah; T Toharmat
Media Peternakan Vol. 37 No. 3 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.215 KB) | DOI: 10.5398/medpet.2014.37.3.161

Abstract

Buffalo’s rumen bacteria (BRB) are potential in digesting fiber feed. BRB already adapted well with low quality forages and agricultural byproducts. The aim of this study was to determine the effect of buffalo’s rumen bacteria (BRB) consortium inoculated into preweaning Frisian Holstein calves on nutrient digestibility, physiological status, mineral uptake, and blood profile. This study used 14 isolates of bacteria isolated from rumen fluid of four local buffalos. The research units consisted of seven Frisian Holstein calves at two weeks old with the average body weight of 43.6±4.5 kg. Calves were inoculated with 20 mL of buffalo’s rumen bacteria isolates [4.56 x 109 cfu/mL] every morning for 10 weeks. The calves were divided into two groups i.e., three calves received bacterial inoculation and four calves without any inoculation. The variables which were analyzed in the preweaning and weaning period were feed intake, digestibility, average daily gain (ADG), feed conversion ratio (FCR), rumen fermentation characteristics, body weight, physiological status, blood profile, and mineral status. Data were analyzed statistically using t-test. The results showed that inoculation of buffalo’s rumen bacteria into Frisian Holstein calves effectively increased feed intake, characteristics of leukocytes and neutrophils, and cobalt (Co) uptake during the weaning period. Inoculation of rumen bacteria improved rumen pH during preweaning and weaning periods. Inoculation of rumen bacteria also had no negative effects on digestibility, feed conversion (FCR), average daily gain (ADG), and physiological status.Key words: buffalo rumen bacteria, digestibility, physiological status, blood profile, Pennisetum purpureum
Performance, Carcass Production, and Meat Quality of Sumba Ongole Bulls Fed Ration Supplemented Velvet Bean (Mucuna pruriens) S. M. Yantika; . Alamsyari; D. Evvyernie; D. Diapari; K. Winaga
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.297 KB) | DOI: 10.5398/medpet.2016.39.1.20

Abstract

This research aimed to study performance, carcass production, meat quality, and economic feasibility of male Sumba Ongole cattle fed ration supplemented with velvet bean (M. pruriens). The research was designed in a completely randomized block design using 16 cattle with average body weight of 488+37.08 kg. The treatments were: T0= 15% straw + 85% concentrate; T1= T0 + 12% of velvet bean flour supplement; T2= T0 + 16% of velvet bean tempeh supplement; and T3= T0 + 200 mg/head/d of ractopamine hydrocloride. Data were analyzed by using analysis of variance with orthogonal contrast. The observed variables included feed consumption, average daily body weight gain (ADG), feed efficiency (FE), income over feed cost (IOFC), hot carcass weight (HCW), carcass percentage, subcutaneous fat thickness, meat pH, tenderness, cooking loss, and water holding capacity (WHC). The result revealed that the addition of 12% velvet bean flour into the rations increased dry matter consumption (P<0.05), but did not affect ADG, FE, IOFC, carcass production, and meat quality. The addition of 12% velvet bean flour produced the lowest cooking loss (P<0.05). The addition of 16% velvet bean tempeh into the rations significantly increased (P<0.05) dry matter consumption, however reduced (P<0.05) ADG, FE, and IOFC compared to control treatment. It is concluded that velvet bean flour at the level of 12% can be used as an alternative feed supplement in the ration of Sumba Ongole cattle and can replace the use of synthetic ß-agonist.
Production and Energy Partition of Lactating Dairy Goats Fed Rations Containing Date Fruit Waste Endah Yuniarti; Dwierra Evvyernie; Dewi Apri Astuti
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (669.563 KB) | DOI: 10.5398/medpet.2016.39.1.27

Abstract

Dates fruit waste (DFW) is a by-product of dates juice industry that contains high energy. So, it is suitable for an energy source in dairy goat ration. This study was conducted to observe the effect of DFW utilization in the ration on energy partition and productivity of lactating dairy goats. The experimental design was randomized block design using 9 primiparous lactating dairy goats. There were three types of ration as treatments used in this study, i.e. R0= 35% forage + 65% concentrate, R1= 35% forage + 55% concentrate + 10% DFW, and R2= 35% forage + 45% concentrate + 20% DFW. Data were analyzed using ANOVA and polynomial orthogonal test. The evaluated variables were dry matter intake (DMI), energy partition including energy intake, digestible and metabolizable energy, fecal and urine energy, energy in methane gas, and energy in milk, milk production and quality. The results showed that the linear decreased of DMI, energy intake, digestible energy, metabolizable energy, and urine energy with the increased of DFW level in the rations. The use of 10% DFW (R1) showed the lowest energy loss through feces and methane gas of all treatments about 1089.57 kcal/head/d and 2.36 kcal/head/d, respectively. The use of DFW did not affect energy retention in milk. The utilization of DFW in ration did not significantly prevent the decline of milk production and milk quality. It can be concluded that DFW can be used as an alternative feed for the lactating dairy goat up to 10% in the ration.
In vitro Fermentation Characteristics and Rumen Microbial Population of Diet Supplemented with Saccharomyces cerevisiae and Rumen Microbe Probiotics Lilis Riyanti; . Suryahadi; Dwierra Evvyernie
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.45 KB) | DOI: 10.5398/medpet.2016.39.1.40

Abstract

The objective of this study was to select three strains of probiotic Saccharomyces cerevisiae and to evaluate the effect of S. cerevisiae and rumen bacteria isolate (MR4) supplementation and their combination on rumen fermentability and rumen microbial population. Experiment 1 was designed in a 4 x 5 factorial randomized block design with 3 replications. The first factor was S. cerevisiae strain consisted of control treatment (without S. cerevisiae supplementation), NBRC 10217, NRRL Y 567 and NRRL 12618, and the second factor was incubation time consisted of 0, 1, 2, 3, and 4 h. Ration was basal ration for feedlot with forage to concentrate ratio (F:C)= 60:40. Dosage of each treatment with S. cerevisiae was 5 x 1010 cfu/kg ration. Experiment 2 was designed in randomized block design with 4 treatments: P0= basal ration of feedlot; P1= P0 + S. cerevisiae; P2= P0 + MR4 isolate (5 x 107 cfu/kg ration); P3= P0 + S. cerevisiae and MR4 isolate. The result of experiment 1 showed that supplementation of S. cerevisiae NRRL 12618 had the highest S. cerevisiae population and increased rumen bacterial population. This strain was selected as probiotic in experiment 2. The result from experiment 2 showed that probiotic supplementation stabilized rumen pH and produced the highest NH3 concentration (P<0.05) and bacterial population (P<0.05). As compared with control, all treatments reduced protozoa population (P<0.05). Combination of S. cerevisiae and MR4 probiotics produced the highest total volatile fatty acids (VFA) and isovalerate (P<0.05). It was concluded that strain S. cerevisiae NRRL 12618 had potential as probiotic yeast. Supplementation with this strain increased fermentability, rumen isoacid and decreased A:P ratio. Those abilities could be improved with MR4 rumen isolate probiotic.
Phytochemical Screening and in Vitro Ovicidal, Larvacidal, and Nematicidal Effects of Murraya paniculata (L.) Jack Extract on Gastrointestinal Parasites of Goats G. E. Tresia; D. Evvyernie; R. Tiuria
Media Peternakan Vol. 39 No. 3 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.715 KB) | DOI: 10.5398/medpet.2016.39.3.173

Abstract

In our previous research, kemuning leaves (Murraya paniculata L. Jack) was shown to have the capability as an anthelmintic candidate for PE (Ettawa crossbred) lactating dairy goats by reducing 43.67% of the egg per gram (EPG) of Strongylida compared to 0.0005% in orally treated with Oxfendazole as a control. To confirm it, the aim of this in vitro study was to determine the effective dosage of kemuning leaves from two extraction methods (infuse and maceration) to reduce the Trichostrongylidae and to evaluate the bioactive compounds of the leaves. The research was conducted using a randomized complete design with 11 treatments and 5 replications. The treatments consisted of control (0.0005% and 0.005% Oxfendazole), kemuning leaves infuse extract (KIE) and maceration extract (KME) each with level of 1%, 3%, 5%, and 7% (w/v). The results showed that the LT50 gradually decreased (shortening the lethal time) and the mortality of Trichostrongylidae gradually increased associated with the increased concentration of treatment (P<0.01). The infusion of 7% kemuning extract demonstrated the highest efficiency in reducing the larval development, infective larvae, and the adult Trichostrongylidae by 93.16%, 94.39%, and 90%, respectively.  This treatment could be developed as the most prospective herbal anthelmintic drug in controlling the infection by Trichostrongylidae.
Legume Wafer Supplementation to Increase the Performance of Post-Weaning Ettawa Grade Goats Brilian Desca Dianingtyas; Yuli Retnani; Dwierra Evvyernie
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.484 KB) | DOI: 10.5398/medpet.2017.40.1.42

Abstract

This research was conducted to analyze the effect of legume wafer supplementation on the performance of post weaning Ettawa Grade goats. A total of 16 post weaning Ettawa Grade goats (average body weight 13.10±0.91 kg) were grouped into 4 group treatments and 4 blocks as replicate in a completely randomized block design. The treatments were T0 (basal diet/control), T1 (supplementation of 12.12% wafer supplement of Indigofera zollingeriana), T2 (supplementation of 13.54% wafer supplement of Leucaena leucocephala) and T3 (supplementation of 12.37% wafer supplement of Calliandra calothyrsus). The feed intake, nutrient digestibility, average daily gain (ADG), feed efficiency (FE), and income over feed cost (IOFC) were observed. The results showed that supplementation of legume wafer increased (P<0.05) the feed intake, organic matter digestibility, ADG, FE, and IOFC. Supplementation of I. zollingeriana wafer increased ADG by 55.08%, FE by 34.91%, and IOFC by 14.53%; L. leucocephala wafer increased ADG by 66.18%, FE by 41.63%, and IOFC by 19.09%; and C. calothyrsus wafer increased ADG by 32.62%, FE by 11.30%, and IOFC by 14.34%. In conclusion, the addition of legume wafer supplements into the rations could increase the feed utility value of legumes and L. leucocephala wafer at 13.54% showed the best performance in post-weaning Ettawa Grade goats.
Ability of fiber bacteria isolated from buffalo rumen in digesting various sources of forage Iwan Prihantoro; T Toharmat; D Evvyernie; Suryani .; L Abdullah
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1447.415 KB) | DOI: 10.14334/jitv.v17i3.699

Abstract

Local buffalo rumen fluid is a source of fiber digesting bacteria. Such bacteria presumably are well adapted to feed stuffs derived from agricultural byproducts with low quality. The purposes of this study were: (1) to isolate the fiber-digesting bacteria from buffalo rumen fluid, (2) to determine the fiber digesting characteristics and adaptability of such bacteria, and (3) to characterize the bacterial diversity.  Rumen fluids for the experiment were collected from buffaloes slaughtered at the Faculty of Animal Science, Bogor Agricultural University abattoir. Fiber-digesting bacteria were isolated using McDougall buffer supplemented with fibrous feed. A factorial experiment was conducted to study characteristics and adaptability of the bacteria using two factors: the type of bacteria and type of feed source of the fiber. Diversity among the bacteria was analyzed using the NTSys2.10 program.Results showed that nine bacterial isolates had a high adaptability to fiber feed based on CMCase. The highest CMCase activity bacteria for Pennisetum purpureum were A9 (11.36±1.70 unit/ml/h), A3 (11.22±0.60 unit/ml/h) and A42 (10.62±1.96 unit/ml/h). CMCase activity of fiber digesting bacteria from buffalo rumen fluid was not correlated with the number of bacteria in the culture. Based on genetic similarity, nine isolates were grouped into five types having similarity≥46%. Key Words: Buffalo Rumen Fluid, Fiber-Digesting Bacteria, Low Quality Forage
Potency of fiber rumen bacterial isolates from local buffalo inoculated into Frisian Holstein calves during preweaning period Iwan Prihantoro; D Evvyernie; Suryani .; L Abdullah; N.S Yunitasari; A.P Sari; D Khairunisa; A Haziq; N Rahayu; T Toharmat
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 4 (2012): DECEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.773 KB) | DOI: 10.14334/jitv.v17i4.696

Abstract

Fiber-digesting bacteria are the main rumen bacteria that play an important role in digesting feed. These bacteria are adapted to low quality forage from agricultural byproduct. The aim of these study was to determine the potency of fiber-digesting bacteria consortium obtained from buffalo rumen inoculated to Frisian Holstein calves during preweaning on feed consumption, utilization, mineral uptake and physiological status. This study used 14 isolates of bacteria obtained from collection of Faculty of Animal Science, Bogor Agricultural University. The experimental unit consisted of six Frisian Holstein calves at two week old with the average body weight of 38.00 ± 6.23 kg. Calves were inoculated by 20 ml of fiber-digesting rumen bacterial isolates [4.56 x 109 cfu/ml] every morning for four weeks. Experimental design used was based on a completly randomized design with three calves received the respective inoculation (treatment group) and three calves without any inoculation (control group). Data were analyzed statistically using t-test method with α = 0.05 and 0.01. The results showed that fiber-digesting bacteria (FDB) from rumen buffalo have adapted in the calves rumen since preweaning periode. Inoculation FDB increased the number of rumen bacteria, digestibility of protein and P uptake calves at eight weeks old. Increased feed intake, uptake of Mg and cobalt calves at 14 weeks old. Without causing any negative effects on ADG, physiological status and rumen fermentability. Key Words: Fiber-Digesting Bacteria, Nutrient Intake, Rumen Fermentability
Detoksifikasi Mikotoksin Melalui Optimalisasi Fungsi Rumen dengan Pemberian Ragi (MYCOTOXIN DETOXIFICATION THROUGH OPTIMIZATION THE RUMEN FUNCTION BY YEAST) Dadik Pantaya; Komang Gede Wiryawan; Dwierra Evvyernie Amirroenas; Suryahadi .
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.305 KB)

Abstract

Mycotoxins are toxic metabolites produced by some fungal species commonly found in food and feed,particularly in cereals. In intensive production systems, dairy cattle are commonly fed with cereal-richdiets and, consequently, are more exposed to micotoxins. Besides, such diet is often associated with ahigher risk of rumen acidosis which can also affect the performance and the helath of animal. In addition,the efficacy of microbial detoxification can be reduced during acidosis. For instance, some authors observeda decrease in the number of protozoa that are responsible for the degradation of some mycotoxins. Anotherconsequence of acidosis is the potential modification of ruminal absorption of mycotoxins, which until nowhas received scarce attention. Yeast Saccharomyces cerevisiae, probiotic additives have been shown toreduce the post-feeding drop in rumen pH and to increase the number of ruminal protozoa. This effect canbe positive in reducing the absorption and toxicity of mycotoxins in ruminantia.
Fermentabilitas Rumen dan Kecernaan In Vitro Ransum yang Disuplementasi Kulit Buah Kopi Produk Fermentasi Jamur Pleurotus ostreatus Irma Badarina; Dwierra Evvyernie; Toto Toharmat Toharmat; Elis Nina Herliyana
Jurnal Sain Peternakan Indonesia Vol 9, No 2 (2014)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.9.2.102-109

Abstract

ABSTRAKPenelitian ini bertujuan mengevaluasi potensi kulit buah kopi yang telah difermentasi jamur Pleurotus ostreatussebagai pakan suplemen secara in vitro berupa tingkat fermentabilitas dalam rumen dan kecernaan bahan kering serta bahan organik ransum. Kulit buah kopi produk biokonversi ditambahkan ke dalam ransum konsentrat sebanyak 0%(R0), 2%(R2), 4%(R4) dan 6%(R6) dari total bahan kering ransum. Rancangan penelitian menggunakan rancangan acak kelompok dan tiga sumber cairan rumen kambing sebagai blok dan level pemakaian kulit buah kopi dalam ransum sebagai perlakuan. Hasil penelitian menunjukkan bahwa perlakuan tidak memberikan pengaruh yang nyata terhadap pH rumen dan NH3-N. Nilai rerata pH rumen dalam penelitian ini berada dalam kisaran normal. Suplementasi sampai level tertinggi (6%) tidak mengganggu metabolisme protein dalam rumen. Kadar VFA nyata menurun pada ransum yang disuplementasi dibandingkan ransum kontrol, dan tidak terdapat perbedaan yang nyata dalam produksi VFA diantara ransum yang disuplementasi. Produksi VFA berada dalam kisaran normal. Perlakuan tidak memberikan pengaruh nyata terhadap populasi mikrobia rumen (protozoa dan bakteri). Hal ini memberikan indikasi bahwa suplementasi kulit buah kopi produk biokonversi sampai level 6% tidak membahayakan kehidupan mikroba rumen. Kecernaan bahan kering dan bahan organik ransum yang disuplementasi menurun dibandingkan ransum kontrol. Level 6% penambahan di dalam ransum, kecernaan masih dikategorikan baik.Kata Kunci : Kulit buah kopi, P.ostreatus, fermentabilitas, kecernaan, rumen