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All Journal Farmaka Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Chimica et Natura Acta Proceedings of the International Conference on Applied Science and Health JCPS (Journal of Current Pharmaceutical Sciences)
Sriwidodo Sriwidodo
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Review Article: Effect of Co-Expression Chaperones on the Expression of Intracellular Recombinant Proteins in Escherichia coli Diandra Firdiani Utami; Mamlikatu Ilmi Azizah; Sriwidodo Sriwidodo; Ryan Adibagus Haryanto; Riyona Desvy Pratiwi; Iman Permana Maksum
Chimica et Natura Acta Vol 11, No 2 (2023)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v11.n2.46480

Abstract

Escherichia coli is widely used as a host for expressing recombinant proteins due to its well-studied genetics, fast growth, relatively low production costs, and high rate of protein expression. However, despite the high rate of protein expression, the availability of chaperone proteins was often insufficient, resulting in the formation of inclusion bodies due to errors in protein folding. These inclusion bodies can cause the protein to become inactive, and proper protein folding is crucial for maintaining the structure and function of proteins in living organisms. To overcome this limitation, chaperones have been developed as a strategy to help prevent protein folding errors and increase the recovery of soluble protein. In this review, we summarize several experiments related to co-expressing chaperones to enhance the expression of recombinant proteins in E. coli.
Co-Authors Ameilia Ameilia AMI TJITRARESMI ANIS YOHANA CHAERUNISA Anis Yohana Chaerunisaa ANTI PEBRIANTI MENTARI ARIDA SAFIRA ARWA ARWA Atharia Refi Khairani Nasution Diandra Firdiani Utami DUDI RUNADI Ellin Febrina FERRY FERDIANSYAH SOFIAN Gofarana Wilar GUSTYAN PRATAMA Handi Purnama I Gede Bayu Somantara Iman Permana Maksum Indah Suasani Wahyuni Irma Erika Herawati Irma Melyani Puspitasari Jutti Levita Mamlikatu Ilmi Azizah Mutakin Mutakin Renny Amelia Riyona Desvy Pratiwi Rosa Riau Wati Ryan Adibagus Haryanto Santosa Santosa SORAYA RATNAWULAN MITA, SORAYA RATNAWULAN Sri Adi Sumiwi Sucilawati Ridwan TIANA MILANDA VALENTINE SOFIANI Yasmiwar Susilawati Zelika Mega Ramadhania