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All Journal Jurnal Agro JURNAL ILMIAH AGRINECA Agricultural Science GANESHA: Jurnal Pengabdian Masyarakat PROFICIO: Jurnal Pengabdian Masyarakat Journal of Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education
Daryanti
Universitas Tunas Pembangunan Surakarta (UTP)
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Engineering Materials Science & Nanotechnology Social Sciences Other

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Utilization of Rhizoctonia Mycorrhizae for Orchid Late Blight Control in Sustainable Agricultural R. Soelistijono; Daryanti Daryanti; Dian Rakhmawati; Prisma Aditya Rianto; Herry Utomo
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3498

Abstract

Vanda tricolor is currently a protected species because is the occurrence of orchid late blight caused by the Fusarium sp. pathogenic fungus. The objective of this study was to investigate the effectiveness of Rhizoctonia mycorrhizae in inducing resistance against Fusarium sp., the causative agent of late blight, in V. tricolor. The research employed CRBD with three treatments and six replications. Rhizoctonia mycorrhizae were isolated and identified following the procedure outlined by Bayman. Pre-inoculation of Rhizoctonia mycorrhizae was conducted on PDA media, and mycorrhizae were subsequently inoculated onto the roots of V. tricolor seedlings derived from tissue culture. The presence of peloton structures on the roots was determined using the Nakano methods. The Saravanan method was employed to measure peroxidase activity. The results demonstrated that V. tricolor induced by Rhizoctonia mycorrhizae exhibited resistance against Fusarium sp. and displayed the formation of peroxidase enzymes, peloton structures, and lignification. In contrast, the non-induced orchids suffered significant damage to the epidermal tissue of the leaves, did not exhibit an increase in peroxidase enzymes, and failed to form peloton or lignified structures. The novelty of this research is the use of Rhizoctonia mycorrhizae to control orchid late blight diseases at Mount Merapi.
Co-Authors Achmad Fatchul Aziez Agung Prasetyo Agus Budiyono Alif Amirul Fatkhurahman Dian Rakhmawati Dian Rakhmawati Dian Rakhmawati Dika Saputra Dwi Susilo Utami Dwi Susilo Utami Endang Suprapti Fatimah Putri Nabila Azzahra HARTOYO, EKO Haryuni Herry Utomo Irvansyah Cahya Perwita K Aprilliyan Kusriani Prasetyowati Kusriani Prasetyowati Mahananto Muharram Indrawan Nendro Asto W Norbertus Citra Irawan Nurul Ida Cahyani ongko cahyono Prisma Aditya Rianto Qoirul Umam R Soelistijono R. Soelistijono R. Sulistijono Sapto Priyadi Setie Harieni Setie Harieni Setie Harieni Siti Mardhika Sari Siti Mardhika Sari Solikhun Sukamto Suswadi Teguh Supriyadi Tyara Yunika Sari Tyas Soemarah K.D Wiyono Yuliana Tri Nugraheni