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Journal : Jurnal Riset Akuakultur

PENGARUH KONSENTRASI LINDI YANG DIFERMENTASI DENGAN AKTIFATOR MIKROORGANISME EM4 TERHADAP KEPADATAN SEL Chlorella sp. Hadi, Khairul; Rosyadi, Rosyadi
Jurnal Riset Akuakultur Vol 17, No 4 (2022): (Desember 2022)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.17.4.2022.215-226

Abstract

Lindi merupakan cairan yang terbentuk dalam timbunan sampah yang kaya akan nutrisi dan dapat digunakan sebagai unsur hara untuk kultur mikroalga jenis Chlorella sp. Penelitian ini bertujuan untuk mendapatkan konsentrasi lindi yang difermentasi dengan aktifator mikroorganisme EM4 terhadap kepadatan sel Chlorella sp. Design experiment yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan tiga ulangan, yaitu P1 konsentrasi lindi 5%, P2 (10%), P3 (15%) P4 (20%), dan P5 (25%). Parameter yang diamati yaitu kepadatan sel, laju pertumbuhan spesifik, kadar nitrat dan fosfat, serta kualitas air (suhu, pH, dan oksigen terlarut). Data dianalisis dengan ANAVA menggunakan software SPSS 25. Kepadatan tertinggi diperoleh pada konsentrasi 25% sebesar 731,1 ± 2,55 ×104 sel mL-1, dengan puncak pertumbuhan pada hari ke-16. Kepadatan sel terendah pada konsentrasi 5% sebesar 256,4 ± 6,25 ×104 sel mL-1, dengan puncak pertumbuhan pada hari ke-6, laju pertumbuhan spesifik tertinggi pada konsentrasi lindi 5% sebesar 0,19 ± 0,00 sel mL-1 hari-1 dan terendah pada konsentrasi lindi 20% sebesar 0,08 ± 0,00 sel mL-1 hari-1. Berdasarkan hasil penelitian dapat disimpulkan bahwa perbedaan konsentrasi lindi yang difermentasi dengan EM4 berpengaruh sangat nyata terhadap produksi Chlorella sp.Leachate is a liquid formed in heaps of waste that is rich in nutrients and could be used to supply the required nutrients in microalgae cultures such as Chlorella sp. This experiment aimed to determine the effect of different concentrations of leachate fermented using EM4 microorganism activator on the cell density of Chlorella sp. The experimental design used a Completely Randomized Design (CRD) with five treatments and three replications: P1 (5% leachate concentration), P2 (10%), P3 (15%), P4 (20%), and P5 (25%). The observed parameters were cell density, specific growth rate, nitrate and phosphate levels, and water quality (temperature, pH, and dissolved oxygen). The data were analyzed using ANOVA using SPSS 25 software. The highest density of Chlorella sp. was 731.1 ± 2.55 ×104 cells mL-1obtained by P5 treatment recorded at the growth peak in the 16th day. The lowest cell density of Chlorella sp. was measured in P1 treatment at 256.4 ± 6.25 ×104 cells mL-1 during the growth peak on day 6th. The highest and lowest specific growth rates of Chlorella sp. were observed in P1 (0.19 ± 0.00 cells mL-1 day-1)and P4 treatments (0.08 ± 0.00 cells mL-1 day-1), respectively based on these results, this recent study concludes that the fermented leachate using EM4 is capable of suppying sufficient nutrient and thus has a very significant effect on the production of Chlorella sp.
PENGARUH PEMBERIAN PAKAN FERMENTASI AMPAS SAGU DAN TEPUNG KEPALA IKAN TERI DENGAN PERSENTASE BERBEDA TERHADAP KELULUSHIDUPAN DAN PERTUMBUHAN BENIH IKAN BAUNG (Hemibagrus nemurus) Agusnimar, Agusnimar; Setiaji, Jarod; Sadikin, Khairil; Marliana, Desi; Cahyo, Firsal Eko; Hadi, Khairul
Jurnal Riset Akuakultur Vol 18, No 2 (2023): (Juni, 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.2.2023.71-80

Abstract

Salah satu ikan air tawar yang bernilai ekonomi tinggi di Asia Tenggara adalah ikan baung (Hemibagrus nemurus). Namun dalam memenuhi permintaan masyarakat, permasalahan yang sering dihadapi pembudidaya adalah besarnya biaya pakan, sehingga perlu adanya pemberian pakan buatan berbahan dasar lokal yang murah dan mudah didapat. Tujuan dari penelitian ini untuk melihat pengaruh pemberian pakan buatan berupa kombinasi fermentasi ampas sagu dan tepung kepala ikan teri (Stolephorus indicus) dengan persentase yang berbeda terhadap kelulushidupan dan pertumbuhan benih ikan baung. Percobaan ini menggunakan rancangan acak lengkap (RAL) satu faktor dengan empat tingkat perlakuan, yaitu P1 pemberian pakan sebanyak 6%, P2 (8%), P3 (10%), dan P4 (12%) dari bobot tubuh ikan uji dengan tiga ulangan. Hasil penelitian menemukan bahwa persentase pemberian pakan uji kombinasi fermentasi ampas sagu dan tepung kepala ikan teri hingga 10 % dapat meningkatkan pertumbuhan benih ikan baung, namun bila ditingkatkan menjadi 12% pertumbuhan mengalami penurunan. Secara statistik persentase pemberian pakan uji berpengaruh nyata (P<0,05) terhadap bobot mutlak dan laju pertumbuhan harian benih ikan baung. Dari hasil penelitian dapat disimpulkan bahwa persentase pemberian pakan fermentasi ampas sagu dan tepung kepala ikan teri sebanyak 10% dari bobot tubuh ikan baung optimal untuk meningkatkan pertumbuhan karena sesuai dengan kebutuhan nutrisi ikan baung. Pakan ini dapat dikembangkan sebagai pakan berbahan baku lokal untuk ikan baung karena mudah diperoleh, harganya murah, dapat menekan biaya produksi, dan tersedia berkesinambungan.One of high value freshwater fish in Southeast Asia is the Asian redtail catfish (Hemibagrus nemurus). However, to meet the public demand, the frequently problem faced by farmers is the high cost of feed, so it is necessary to provide artificial feed based on local ingredients that are cheap and easy to obtain. The aim of this study was to determine the effects of feeding of an artificial feed in the form of a combination of fermented sago dregs and anchovy head meal (Stolephorus indicus) at different percentages on the survival and growth of the Asian redtail catfish. This study used a one-factor completely randomized design (CRD) with four treatment levels, namely P1 feeding as much as 6%, 8% (P2), 10% (P3), and 12% (P4) of the test fish's body weight triplicates. The results of this study showed that feeding of the test feed up to 10% could increase the growth of Asian redtail catfish fry, but when it was increased to 12%, growth decreased. Statistically, the percentages of feeding of fermented sago dregs and anchovy head meal as much as 10% of Asian redtail catfish body weight was optimum to increase, because it meets the nutritional requirements of Asian redtail catfish (P<0.05). From the results of the study, it can be concluded that the percentage of feeding fermented sago waste and anchovy head meal as much as 10% of the body weight of Asian redtail catfish is optimal for increasing growth because it is in accordance with the nutritional needs of Asian redtail catfish. This feed can be developed as the feed based on local ingredients for Asian redtail catfish because it is easily obtained, the price is cheap, it can reduce production costs, and it is available continuously.