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Pemanfaatan Sari Belimbing Wuluh (Averrhoa bilimbi L.) pada Pemeliharaan Udang Vaname (Litopenaeus vannamei) untuk Menekan Populasi Bakteri Vibrio sp. Koloni Hijau Handayani, Sri; Dwinanti, Sefti Heza; Hadi, Purnomo
Jurnal Masyarakat Akuakultur Indonesia Vol 3, No 1 (2019): In Press
Publisher : Masyarakat Akuakultur Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.238 KB)

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Sri Handayani, Sefti Heza Dwinanti and Purnomo Hadi. 2020. Utilization of Carambola Sari (Averrhoa bilimbi L.) in the Maintenance of Vaname Shrimp (Litopenaeus vannamei) to Suppress Population of Vibrio sp. Green Colony. Jurnal Sains Teknologi Akuakultur, 3(1) : 33-41. This research was conducted in July-August 2018 at PT. SyAqua Indonesia Serang, Banten. It used Completely Randomized Design (CRD) with three replicates and three treatments which were bilimbi juice in 20 ppm (T1), 30 ppm (T2) and without bilimbi or control (CT). The monitoring of Vibrio sp., defined as total Vibrio sp., total   yellow colony, total green colony, the ratio of total green colony, survival rate of shrimp and water quality. The result showed that bilimbi juice was able to suppress the population of Vibrio sp. in the maintenance media and shrimp body. At the end of the maintenance period (d7-d9), total population of green colony in treatment were lower than control with the ratio of the green colony was 0%. In contrast, bilimbi juice with concentration 20 ppm and 30 ppm had no significant effect (P>0,1). The best survival rate was given by T2 about 16% and based on regression y = 0.3589x+5.0279 the optimal dose to protect shrimp from Vibrio sp. by using bilimbi juice is about 264,62 ppm.
In vitro analysis: inhibitory effect of Phyllanthus niruri extract against Aeromonas salmonicida Sefti Heza Dwinanti; Maya Anggita Savacka; Ade Dewi Sasanti
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152109548-0-00

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The purpose of this research is to explore effectiveness from every part of Phyllanthus niruri such as leaves (L), fruits (F), stem (S) and combination (C) of those parts that able to inhibit Aeromonas salmonicida growth by using different methods of extraction both infundation and maceration. Experiments has been done at Budidaya Perairan Laboratory, Sriwijaya University. In vitro analysis used tetracycline 30 µg/ml as positive control, bacterial culture at 1014 CFU/ml and concentration of each part of stone breaker was 20%, 40%, 60%, 80% and 100%. The result revealed that stone breaker extract produced by infundation and maceration methods exhibited inhibitory activity against A.salmonicida. Moreover, only maceration method had bactericidal effect, in fact fruits (F) and combination (C) was the best part of P. niruri which had higher inhibition zone than control (antibiotics). It was 16,9 mm and 17,3 mm respectively. However, the extracts are recommended to cure aeromonad infection due to bacterial resistance of tetracycline already increase. Thus, some extraction methods are needed to be developed for further research with minimum inhibition zone is 20 mm.Penelitian ini bertujuan untuk mengetahui efektivitas setiap bagian Phyllanthus niruri seperti daun (L), buah (F), batang (S) dan kombinasi (C) yang mampu menghambat pertumbuhan Aeromonas salmonicida menggunakan metode yang berbeda, ekstraksi infundasi maupun maserasi. Percobaan telah dilakukan di Laboratorium Budidaya Perairan Universitas Sriwijaya. Analisis in vitro menggunakan tetra siklin 30 g/ml sebagai kontrol positif, kultur bakteri pada 1014 CFU/ml dan konsentrasi masing-masing ekstrak adalah 20%, 40%, 60%, 80% dan 100%. Hasil penelitian menunjukkan bahwa ekstrak yang dihasilkan dengan metode infundasi dan maserasi menunjukkan aktivitas penghambatan terhadap A.salmonicida. Selain itu, hanya metode maserasi yang memiliki efek bakterisidal, ternyata buah (F) dan kombinasi (C) merupakan bagian terbaik dari  P. niruri yang memiliki zona hambat lebih tinggi daripada kontrol (antibiotik). Masing-masing 16,9 mm dan 17,3 mm. Namun, ekstrak tersebut direkomendasikan untuk menyembuhkan infeksi aeromonad karena resistensi bakteri terhadap tetra siklin sudah meningkat. Oleh karena itu, perlu dikembangkan beberapa metode ekstraksi untuk penelitian selanjutnya dengan zona hambat minimal 20 mm.
TOKSISITAS DAN IMUNOGENISITAS PRODUK EKSTRASELULER Streptococcus agalactiae TIPE NON-HEMOLITIK PADA IKAN NILA (Oreochromis niloticus ) Sefti Heza Dwinanti; Sukenda .; Munti Yuhana; Angela M Lusiastuti
Jurnal Akuakultur Rawa Indonesia Vol 2, No 1 (2014): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.092 KB) | DOI: 10.36706/jari.v2i1.2058

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ABSTRACT The aim of this research was to analyse the toxicity and immunogenicity of ECP of non-hemolytic S. agalactiae and to evaluate ECP as a material vaccine for prevention of S. agalactiae. Toxicity to tilapia was detected in the ECP. There was a chronic  mortality pattern which was shown after post ECP injection. By intraperitoneal injection into young tilapia (± 20 g), the median lethal dose were calculated at 633,9 µg/kg bodyweight for isolate 3 and 685,4 µg/kg bodyweight for isolate 5. Cytopathic effects of ECP to tilapia’s lymphocytes were observed during 0, 30, 60 and 180 minutes. A mixture of ECP caused nuclear granulation and cytoplasmic streaming after 60 minutes. Using immunodiffusion analysis, precipitate line was demonstrated against tilapia anti-ECP sera, and there was bio-specific type anti sera against for each isolate. The ECP vaccine effective to protect tilapia from S. agalactiae was injected intraperitoneally (IP) at 2 x 105 CFU/ml. Tilapia vaccinated with ECP from isolat 3 which protein  283 µg/kg bodyweight had a RPS value 60%.  Tilapia vaccinated with ECP from isolat 5 which protein 408 µg/kg  had a RPS value 68%.   Keywords:  toxicity, immunogenicity, extracellular products, Streptococcus agalactiae, tilapia
Pemanfaatan Vitamin C Untuk Meningkatkan Performa Imunitas Benih Ikan Gabus (Channa Striata) sefti heza dwinanti; Ade Dwi Sasanti
Jurnal Akuakultur Rawa Indonesia Vol 7, No 1 (2019): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.072 KB) | DOI: 10.36706/jari.v7i1.9077

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Ascorbic acid was one of the important nutrients for fish because it has the property as an antioxidant and imunomodulator that can improve the performance of fish. The purpose of this research is to support the production of snakehead fish (Channa striata) that have good growth and immunity. This research used a complete randomized design (CRD) consisted of 3 treatments and 3 replications. Ascorbic acid treatment consisted of P1 (0 mg.kg-1), P2 (50 mg.kg-1) and P3 (100 mg.kg-1). The results showed that the maintenance of snakehead fish (Channa striata) given ascorbic acid  with a doses of 100 mg.kg-1  for 30 days gave the best growth results and significantly different and absolute weight growth reached 1.83 g, absolute length of 18.7 mm and survival reached 95 , 83 %. For immunity performance, it can be seen from survival of fish exposed to salinity 20 ppt during 15 minute reach 73,3 % and formalin 10 % for 10 minute reach 66,7 % and disease prevalence percentage reach 16,7 %
MODIFIKASI MEDIA SPIRULINA PLATENSIS SEBAGAI UPAYA PEMANFAATAN AIR LIMBAH BUDIDAYA IKAN LELE Hendro Widyantoro; Marini Wijayanti; Sefti Heza Dwinanti
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.565 KB) | DOI: 10.36706/jari.v6i2.7159

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ABSTRACTThe purpose of this study was to determine the best fertilizer compotition that was made from technical fertilizer and waste water of cat fish farming. This experimental was conducted at Aquaculture Laboratory, Faculty of Agriculture, Sriwijaya University. It used complete randomized design (CRD) method which had five treatments and three replications, the waste water from catfish pond (P0), waste water from catfish pond + 25% technical fertilizer (P1), waste water from catfish pond + 50% technical fertilizers (P2), the waste water from catfish pond + 75% technical fertilizers (P3), the waste water from catfish pond + 100% technical fertilizers (P4). The results showed that the best maximum density of S. platensis was P3 about 3.98 g l-1, and growth rate was equal to 2.80% day-1. The most significant parameter of water quality was ammonia which decreased until 82.78%. Besides, it was more beneficial than others based on Return Cost Ratio (R/C ratio) and Benefit Cost Ratio (B/C ratio) which were 3.66 and 2.66 respectively. Keywords: Spirulina platensis, waste water pond of catfish, modified  media
PENGGUNAAN EKSTRAK AKAR TUBA (Derris elliptica) DENGAN DOSIS YANG BERBEDA DALAM PENGANGKUTAN IKAN PATIN SISTEM TERTUTUP Dini Puspa Monica; Mochamad Syaifudin; Sefti Heza Dwinanti
Jurnal Akuakultur Rawa Indonesia Vol 8, No 1 (2020): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.246 KB) | DOI: 10.36706/jari.v8i1.11164

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The transportation process can lead to stress which increases mortality in fish. Efforts to reduce mortality in the process of fish transportation can be conducted by minimizing the metabolic activities of fish during transportation. One of the natural substances that is used for fish transportation is tuba roots. The aim of this study is to determine the effect of different dosages of tuba root extract on the transport of catfish seeds with a closed transport system for 12 hours. This research was conducted at the Aquaculture Laboratory, Aquaculture Study Program, Faculty of Agriculture, Sriwijaya University. The research was conducted in April 2019. This study used a completely randomized design (CRD) method with four different dosage treatments (T) and three replications. The dosages of tuba root extract were: 0 ml.L-1 (T0), 0.05 ml.L-1 (T1), 0.10 ml.L-1 (T2), 0.15 ml.L-1 (T3). The parameters observed were blood glucose levels, water quality, survival of catfish after transportation and maintenance for 7 days. The results showed that T1 (0,05 ml.L-1) was gave the highest survival rate of 91.66±2.88%, with the lowest stress level indicated by blood glucose values 89±30.63. In addition, there was no further effect from the administration of tubal root extracts to protect the life of the fish after transportation and maintenance.Keywords : Tuba root, catfish, transportation
Pemanfaatan Madu Sebagai Bahan Ekstender Untuk Kriopreservasi Sperma Ikan Gabus (Channa Striata) Anugerah Al-Amin Mangkunegara; Sefti Heza Dwinanti; Mochamad Syaifudin
Jurnal Akuakultur Rawa Indonesia Vol 7, No 2 (2019): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.86 KB) | DOI: 10.36706/jari.v7i2.9916

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Cryopreservation of channa is needed to improve reproduction quality of seeds and broodstock. Some components are important for cryopreservation including quality and quantity of extender. The aim of this study was to investigate the influence honey as extender at different ratio on channa sperm cryopreservation quality. The research used response surface methodology (RSM) with three treatments (T); 300 mg. ml-1 (T1), 400 mg. ml-1 (T2), 500 mg. ml-1 (T3), which all treatments were replicated 3 times. The processed semen was equilibrated at 5°C for 3 h (chilling), packed into straws (0.25 ml), frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Parameters observed were sperm characteristics such as (color, pH, concentration and sperm volume), motility and viability before and after cryopreservation. The results of this study indicated best treatment was honey concentration at 400 mg.ml-1 (T2). After 7 days preservation, the motility value was about 40 – 70 % (4 score), viability percentage was 64.15% and no abnormality was occurred on channa sperm.
PEMANFAATAN AIR KELAPA (Cocos nucifera) UNTUK MASKULINISASI IKAN GUPPY (Poecilia reticulata) Sefti Heza Dwinanti; M. Hanggara Putra; Ade Dwi Sasanti
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.787 KB) | DOI: 10.36706/jari.v6i2.7155

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ABSTRACT               Masculinization is a method of  sex reversal technique that can direct sexuality from female to male. The purpose of this study was to determine the effects of coconut water on masculinization in guppy by immersion administration. The study used a completely randomized design (CRD) with three treatments and three replications. After 12 days post spawning, female guppies were immersed for 8 hours.The concentrations were : 0%(P0), 20% (P1), and 40% (P2). The parameters were observed including percentage of male, survival rate and water quality (thermal, pH and kalium concentration). Baby guppies were reared until sex can be determined which were 42 days of culture. The result showed that the percentage of male guppy fish was 54.44% (P0), 77.78% (P1) and 90.00% (P2). The survival rate of  larval during maintenance was 100% for all treatments. Water quality during maintenance was 27.0-28.40C for temperature and 6.38-7.20 for pH. To sum up, this experiment proved that coconut water can be used for masculinization by immersed pregnant female broadstock and it produced 90% male when immersed with 40% of coconut water. Keywords : Coconut water, guppy (Poecilia reticulata), masculinization
PENERAPAN TEKNOLOGI BUDIDAYA IKAN LELE BIOFLOK SEBAGAI UPAYA PENINGKATAN PENDAPATAN MASYARAKAT DI DESA PANDAN ARANG, KABUPATEN OGAN ILIR Madyasta Anggana Rarassari; Marini Wijayanti; Sefti Heza Dwinanti; Retno Cahya Mukti; Danang Yonarta
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5 No 1 (2021)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/logista.5.1.75-80.2021

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Biofloc Technology (BFT) menjadi salah satu teknologi alternatif dalam akuakultur yang bertujuan untuk memperbaiki kualitas air dan meningkatkan efesiensi pemanfaatan nutrisi. Tujuan dari Pengabdian Kepada Masyarakat ini adalah untuk memperkenalkan dan memberikan informasi kepada masyarakat dan petani tentang budidaya ikan Lele dengan sistem bioflok. Kegiatan ini dilakukan di Desa Pandan Arang, Kabupaten Ogan Ilir dengan menggunakan metode pemberdayaan partisipati aktif kelompok pembudidaya ikan pada mitra yang diajak kerjasama dengan transfer ilmu dan teknologi, meliput beberapa tahapan yaitu: 1) Persiapan; 2) Penyuluhan pembuatan pakan dan budidaya ikan; 3) Pembuatan demplot dan pendampingan; 4) Evaluasi dan pembuatan laporan hasil kegiatan. Hasil yang didapat bahwa kegiatan budidaya ikan lele dengan menerapkan sistem bioflok memberikan hasil dapat memperbaiki kualitas air pada budidaya ikan lele, terutama dalam menurunkan nilai ammonia dalam media budidaya. Serta memberikan pertumbuhan optimal pada ikan lele dengan bobot mutlak 8,4 gram/ekor dan pertumbuhan panjang mutlak 8,6 cm/ekor. Kata kunci: Biofloc Technology, Ikan Lele, Desa Pandan Arang, Budidaya Ikan ABSTRACT Biofloc Technology (BFT) is an alternative technology in aquaculture that aims to improve water quality and increase the efficiency of nutrient utilization. The purpose of this social dedication are to introduce and provide information to the public and farmers with biofloc system. This activity is carried out in Pandan Arang Village, Ogan Ilir District with an active participatory empowerment method for farmers fish in partners who are invited to collaborate with the transfer of knowledge and technology, covering several stages, 1) preparation; 2) counseling for feed and fish cultivation; creating demonstration plots and assistance; 4) evaluation and making reports on the results of activities. The results showed that catfish culture using the biofloc technology could improve water quality in catfish aquaculture, particularly in reducing the value of ammonia in the culture medium. As well as providing optimal growth in catfish witn an absolute weight of 8,4 gr and absolute length growth of 8,6 cm. Keywords: Biofloc Technology, Catfish, Pandan Arang Village, Aquaculture
Pengenalan dan Pelatihan Fish Finder kepada Masyarakat Nelayan di Desa Sungsang IV Kabupaten Banyuasin Sumatera Selatan Beta Susanto Barus; Riris Aryawati; Muhammad Hendri; Andi Agussalim; Gusti Diansyah; Sefti Heza Dwinanti
Jurnal Pengabdian Masyarakat (abdira) Vol 2, No 1 (2022): Abdira, Januari
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v2i1.82

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Sungsang IV Village is a village where most of the residents work as fishermen. The waters around this village have great fishery potential. The results of fish catches by fishermen are felt to be less than optimal due to a lack of knowledge about the latest technology in fishing. Fish Finder is one alternative that can be given to solve this problem because this tool can function to detect the presence of fish. Most of the residents of Sungsang IV Village don't know about the Fish Finder tool. This service activity aims to provide introduction and training regarding the use of Fish Finder to detect the presence of fish. The enthusiasm of the community is very visible from the number of training participants that exceeds the targeted number. This training is carried out by presenting material and direct practice of using the tool to make it easier for participants to understand the operating steps. The results of the evaluation of the questionnaire conducted showed that all participants felt very helpful with the training in the use of this Fish Finder tool.