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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioma : Berkala Ilmiah Biologi The Journal of Experimental Life Sciences (JELS) Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ZOO INDONESIA Biosaintifika: Journal of Biology & Biology Education Patrawidya: Seri Penerbitan Penelitian Sejarah dan Budaya Zoo Indonesia
AMIR HAMIDY
Bidang Zoologi Pusat Penelitian Biologi-Lembaga Ilmu Pengetahuan Indonesia, Bogor
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Social Sciences Other

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Microsatellite Marker for Cross-Species Amplification: Study Case for Indonesian Sundaland Python (Serpentes: Pythonidae) Maulidi, Andri; Fatchiyah, Fatchiyah; Hamidy, Amir; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1180.778 KB) | DOI: 10.21776/ub.jels.2018.008.01.10

Abstract

The python of Indonesian Sundaland has been traded for its distinct skin colour and patterns. The need for rapid method in cross-species amplification for Indonesian Sundaland python is useful to contribute in management of sustainability harvesting system. In this research, we screened 10 microsatellite primers which are previously used for Australian, New Guinean, Chinese and Burmese pythons and 7 potentially amplifiable primers for African and Asian reptiles. Python breitensteini showed a greater number of alleles (2-8 alleles) than Python bivittatus (1-3 alleles) and Python brongersmai (1-2 alleles). The observed and expected heterozygosity for all species were ranged from 0 to 1.00 and 0 to 0.79, respectively. According to the high cross-species amplification rates, 15 out of 17 primers were useful in assessing the genetic diversity and conservation genetic of Indonesian Sundaland python. Among the 15 primers, MS3 generated the highest number of allele for P. breitensteini (8 alleles), P. bivittatus (3 alleles), and P. brongersmai (2 alleles). We proposed MS3 locus as a suitable marker for Indonesian Sundaland python.Keywords: microsatellite, Python, Sundaland.
The Morphological Characters of The Malayan Pit Viper Calloselasma rhodostoma (Kuhl, 1824): on The Cephalic Scalation and Distribution Status in Indonesia Kadafi, Ahmad Muammar; Hamidy, Amir; Kurniawan, Nia
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1346.095 KB) | DOI: 10.21776/ub.jels.2018.008.03.10

Abstract

The examination on variations of morphological characters among 35 specimens of Calloselasma rhodostoma (Kuhl, 1824) from four different populations in Indonesia has been completed in this study. Univariate and multivariate analyzes allowed us to recognize the clustering of four populations through morphological diagnosis. The results of the average body size (Total Length) showed that the largest male is from Kangean Island (579.33 mm), while the largest female is from Java (841.07 mm). Comparison of meristic analysis represented three clusters from Principal Component Analysis (PCA) which is considered to be independent population. Here we also described three types of cephalic scalation variation that called small accessories scales and their distribution in Indonesia.  Keywords: C. rhodostoma, Indonesia, Meristic, Morphometry, Viperidae.
Co-Authors ACHMAD FARAJALLAH Ahmad Muammar Kadafi Aji, Katon Waskito Andri Maulidi Ardiantoro, Ari Atmaja, Vestidhia Yunisya Bagus Priambodo Bambang Agus Suripto Berry Fakhry Hanifa Djong Hon Tjong Donan Satria Yudha Endarwin, Wempi Fahmi, Muhamad Farid Akhsani fatchiyah . Fathoni, M Faturahman, Ahmad Nabil Fauzan, Muhammad Fakhri Fauzi, Luthfi Fauzi, Muhammad Alif Firdaus, Anggun Sausan Fitriyana, Nurul GONO SEMIADI Haekal, Muhammad Hellen Kurniati, Hellen Herlambang, Alamsyah E. N. Karyadi Baskoro Laksono, Wahyu Tri Mahmudul Hasan, Mahmudul Margareta Rahayuningsih Nugroho Edi K Masayuki Sumida, Masayuki Matsui, Masafumi Matsui, Masafumi Mediyansyah Mediyansyah Misbahul Munir Mochamad Hadi Mumpuni Mumpuni Ni Luh Putu Rischa Phadmacanty Nia Kurniawan Pradana, Tengku Gilang Riyanto, Awal Rury Eprilurahman Septiadi, Luhur Smith, Eric N. Smith, Eric N. Smith, Eric Nelson Takeshi Igawa, Takeshi Tesri Maideliza Tuty Arisuryanti, Tuty Vera Budi Lestari Sihotang, Vera Budi Lestari Wahyu Prihatini Winda Rahmawati Wiradarma, Huda Zakky, Quraisy