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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES Jurnal Veteriner Prosiding Seminar Biologi Jurnal Kedokteran Brawijaya BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Biomedical Engineering Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology JURNAL BIOLOGI INDONESIA Jurnal Penelitian Sains PROSIDING SEMINAR NASIONAL Indonesian Journal of Mollusk Proceeding Biology Education Conference Berkala Ilmiah Biologi
Niken Satuti Nur Handayani
Fakultas Biologi, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

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Analisis Pedigree dan Fenotip Pasangan Kembar: Studi Kasus Pada Keluarga Kembar di Kecamatan Laweyan, Surakarta Yuyun Machwiyah; Niken Satuti Nur Handayani
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 1 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i1.443

Abstract

The twin pregnancy is a kind of pregnancy with two or more fetuses. Basically, the twins can be grouped of two, they are fraternal twins (dizygotic) and identical twins (monozygotic). Fraternal twin is a kind of twins that comes from two ova, which each of them will be fertilized by the sperm. While, identical twin comes from an ovum, which fertilized by a sperm. This research is conducted in order to observe the inheritance pattern from the pedigree chart, and to observe similarity and dissimilarity phenotype between twin couple on families observed. Methods used for this research are data collection to construct pedigree, phenotype analysis to compare the similarity and dissimilarity phenotype between the twins that are observed, paraffin method to observe the form of hair from cross section and agglutination reaction to observe the blood group from the twins. Based on pedigree chart of twins in Laweyan, Surakarta, it seems that the identical twins is passedfrom generation to generation, and on the family observed the inheritance pattern of identical twins birth process is come from the women of identical twins. The lobe of ear from each twins there is a twin couple showing the different lobe. There is any similarity and dissimilarity relate to form of hair cross section from each couple. The blood group of each couple are showing the same result.Keywords: identical twins, inheritance pattern, pedigree chart
CD35 (DEL C) frameshift mutation in exon 2 of β-globin gene on β-thalassemia carriers Niken Satuti Nur Handayani
Biomedical Engineering Vol 1, No 1 (2015): BIOMEDICAL ENGINEERING
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.374 KB)

Abstract

Objective : Our work aimed to determine the type and site of mutations of two β-thalassemia carrier sby usinga sequencing method .Methods: DNA genomes were extracted from both carriers blood samples which, by PCR-SSCP analysis, weresuspected of having mutation in exon2 of β-globin gene. The extracted DNA were used as template foramplification of the exon by using specific primers. Amplicon were then read by using the Sanger(dideoxynucleotide chain terminator) method with the reverse and forward primers. Sequencing data wereanalyzed by using software Chromas Bioedit Lite2.1 and 7.1.9Result : The results of the analysis showed the presence of C deletion at codon 35 (Cd 35(del C)) which causesa codon frameshift on both carriers.Conclusion : NLS extract and fractions have high SOD antioxidant value but low DPPH scavenging activity andcan be used as potential hypoglycemic agent.
Phenotype of Transgenic Tobacco Plants (Nicotiana tabacum cv. Petit Havana SR-1) Expressing 1724orf13 Gene of Agrobacterium rhizogenes strain MAFF301724 Niken Satuti Nur Handayani; Nobukazu Tanaka; Kazuo Yoshida
Indonesian Journal of Biotechnology Vol 12, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.365 KB) | DOI: 10.22146/ijbiotech.7771

Abstract

Nicotiana tabacum cv. Petit Havana SR-1 transgenic plants expressing ORF13 of Agrobacterium rhizogenes strainMAFF301724 under different promoters displayed plant morphology abnormalities. They were small, with shortand variable internodes lengths; leaves were small, asymmetric, rounded, wrinkled and dark green; flowers wereshort, and irregularly shaped. This phenotype was also exhibited, similar, but not completely the same, to those ofhairy root syndrome, indicating that expression of ORF13 influences plant development.Keywords: ORF13, Agrobacterium rhizogenes strain MAFF301724, transgenic plants, morphology abnormalities
Keragaman Genetik Gen Penyandi Dehydrogenase Sub-unit 3 Mitokondria pada Monyet Hantu (Tarsius sp.) (GENETIC DIVERSITY OF MITOCHONDRIAL DEHYDROGENASE SUB-UNIT 3 GENE ON TARSIUS SP.) Rini Widayanti; Niken Satuti Nur Handayani; I Made Budiarsa
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Tarsius is an endemic species in Indonesia but is is getting threatened. In-situ and ex-situ conservationof this species would yield better results when its genetic information and diversity determined. Theobjective of this ressearch was to study the genetic diversity of ND3 gene of Tarsius sp. Based on sequencingof PCR products using primer ND3F and ND3R resulted in 437 nts base sequence. Results of ND3 fragmentssequencing were put on multiple alignment with other primates from Genbank using of software ClustalW, and then were analyzed using MEGA program version 4.1. A different nucleotide site was found(nucleotide no. 24). The genetic distance based on nucleotide ND3 was calculated using Kimura 2-parametermodel. The genetic distance showed the smallest genetic distance 0%, the biggest 0,03% and average0,01%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide ND3 genecould not be used to differentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from NorthSulawesi) and T. bancanus (from Lampung, South Sumatera).
Keragaman Genetik Sekuen Gen ATP Synthase FO Subunit 6 (ATP6) Monyet Hantu (Tarsius) Indonesia (GENETIC DIVERSITY STUDY OF ATP6 GENE SEQUENCES OF TARSIERS FROM INDONESIA) Rini Widayanti; Niken Satuti Nur Handayani; Hery Wijayanto
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.212 KB)

Abstract

In a conservation effort, the identification of Tarsier species, on the bases of the morphological andmolecular characteristic is necessary. Up to now, the identification of the animals were based on themorphology and vocalizations, which is extremely difficult to identify each, tarsier species. The objective ofthis research was to study the genetic diversity on ATP6 gene of Tarsius sp. Based on sequencing of PCRproduct using primer ATP6F and ATP6R with 681 nts. PCR product. The sequence of ATP6 fragmentswere aligned with other primates from Gene bank with aid of software Clustal W, and were analyzed usingMEGA program version 4.0. Three different nucleotide sites were found (nucleotide no. 288, 321 and 367).The genetic distance based on nucleotide ATP6 sequence calculated using Kimura 2-parameter modelindicated that the smallest genetic distance 0%, biggest 0.8% and average 0, 2%. The phylogenetic treeusing neighbor joining method based on the sequence of nucleotide ATP6 gene could not be used todifferentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from North Sulawesi), T. bancanus(from lampung, South Sumatera) and T.bancanus from West Kalimantan.
MUTASI MISSENSE (P.374PHE/LEU) PADA EKSON 5 GEN MATP, PENYEBAB OCULOCUTANEOUS ALBINISM TIPE 4 (OCA4) DI WONOSOBO, JAWA TENGAH Niken Satuti Nur Handayani; Feri Sukmawati; Rarastoeti Pratiwi
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 8, No 1 (2011): Prosiding Seminar Nasional VIII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

ABSTRAK Albinisme merupakan kelainan genetik autosomal resesif  berupa gangguan sintesis melanin yang terjadi pada manusia. Albinisme dibagi menjadi dua kelompok besar, yaitu Ocular Albinism (OA) dan Oculocutaneous Albinism (OCA). Berdasarkan gen yang mengalami mutasi, OCA dibedakan menjadi 4 tipe yaitu OCA1, OCA2, OCA3 dan OCA4. OCA4 disebabkan mutasi pada gen MATP. Penelitian yang telah dilakukan dengan PCR-SSCP (Polymerase Chain Reaction-Single Stranded Conformation Polymorphism) mendeteksi adanya mutasi pada ekson 5 gen MATP, pada penderita albinisme di Wonosobo, Jawa Tengah. Sekuensing ekson 5 gen MATP dilakukan untuk mengidentifikasi tipe mutasinya. DNA diisolasi dari sampel darah penderita dan digunakan sebagai template untuk amplifikasi ekson 5 gen MATP dengan metode PCR. Produk PCR selanjutnya digunakan sebagai template untuk sekuensing dengan metode Sanger. Hasil sekuensing dianalisis menggunakan program Clustal-W dan dibandingkan dengan sekuens ekson 5 gen MATP dari International DNA Data Base (nomer akses AF172849.1). Hasil analisis menunjukkan adanya pada perubahan basa nukleotida no.1122 dari C menjadi G (c.1122 C>G) yang mengakibatkan mutasi missense, yaitu fenilalanin menjadi leusin, pada asam amino nomer 374 (p.374 Phe/Leu).   Kata kunci : OCA4, mutasi, ekson 5, gen MATP
Cephalometry Variation of Bataknese Maria Natalina Lumban Gaol; Niken Satuti Nur Handayani
Berkala Ilmiah Biologi Vol 13 No 1 (2022): Jurnal Berkala Ilmiah Biologi
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v13i1.4100

Abstract

Indonesia is a country that consists of various ethnic groups, one of the largest ethnic groups in Indonesia is Bataknese. Bataknese divided into several sub-ethnics, with the largest sub-ethnics are Batak Toba, Batak Karo and Batak Simalungun. This research aims to analyze cephalometric variation of Bataknese. An increase of various ethnic groups in various regions, followed by an increase in traffic activity and the quantity of crime can lead to an increase of traffic accidents and crime victims. Therefore, this research needs to be carried out to determine cephalometric variations and cephalometric index in the Bataknese which can be used in the process of revealing the identity of crime victims and traffic accidents. This research involved 60 subjects of Bataknese, consisting of 20 people of Batak Toba, 20 people of Batak Karo, and 20 people of Batak Simalungun. The method in this research consisted of submit ethical clearance, subject data collection, subject measurement, cephalometric index calculation, and data analysis. The results showed that Batak Toba, Karo, and Simalungun have brachycephalic head type, wide forehead type, and platyrrhine nose type. Batak Toba and Batak Karo are known to have a leptoprosopic facial type, while Batak Simalungun has mesoprosopic facial type. In this research, it was also showed that between Batak Toba and Batak Karo there were no significant differences in all body size and cephalometric data, but between Batak Toba and Batak Simalungun there were significant differences in minimum frontal width, nose width, and frontoparietal index, while between Batak Simalungun and Batak Karo there were significant differences in minimum frontal width and facial width.
Diversity of Bivalves in Cengkrong Mangrove Trenggalek, East Java Based on Morphological and Molecular Character Mukhlis, Mukhlis; Niken Satuti Nur Handayani; Trijoko
Jurnal Moluska Indonesia Vol. 8 No. 1 (2024): April 2024
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v8i1.75

Abstract

Bivalves in mangrove ecosystem plays an important role in the process of litter decomposition and mineralization of organic matter, especially that are herbivores and detrivor. Bivalves in one ecosystem can be known by looking the morphologic character, while the molecular character can be use to know the genetic variation of species in population. The purpose of this research was to determine species diversity of bivalve, and to understand the morphological and molecular characters of them. Phenetic Similarity analyzed using 109 morphological characters, ISSR 6 ISSR 8 primer. The results were analyzed using MVSP 3.1 (Multi-Variate Statistical Package) program to obtain a dendogram that show similarity of bivalves. Four species of bivalves identified, consisting of Polymesoda Erosa, Meretrix meretrix, Pholas sp., and Glauconome virens. Based on morphological and molecular analysis showed two main clusters with the similarity of 54.8% for morphology and 57.6% for molecular. Based on analysis of morphological and molecular characters, it can be concluded that the diversity of the bivalves in ​​mangrove forests Cengkrong is influenced by morphological structure, meristik, morphometric shell, and the type of habitat.
Desain Mini Primer STR Lokus TH01 dan D21S11 untuk Amplifikasi DNA Darah Terpapar Suhu Tinggi Nur Handayani, Niken Satuti; Pertiwi, Ivory Martha; Nugraheni, Kireida Asta
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 13 No 2 (2023): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2023.v13.i02.p05

Abstract

DNA identification has an important role in forensics to investigate a case. However, the problem that is often encountered is that the DNA found is difficult to analyze because the amount is relatively small and degraded. An alternative used in DNA examination is to design mini primers for amplification. Mini primers can produce DNA profiles even in samples that have very low quantity and quality. Therefore, this study aims to determine the effectiveness of mini primers for amplification of TH01 and D21S11 loci in DNA exposed to high temperatures. In addition, to determine the effect of high temperature exposure on DNA quality and quantity. The samples used were 3 ml of human blood treated with 100, 150, and 200ºC for 10, 20, and 30 minutes. The method used was PCR amplification using mini primers TH01 and D21S11 loci. Quantity data were analyzed by a parametric test using the Two Way Anova method. Meanwhile, DNA quality was tested by agarose gel electrophoresis. The results showed that temperature treatment affected the quantity and quality of DNA. The TH01 locus in DNA samples treated with high temperature can be amplified using primer pairs MP1FTH01 and MP1RTH01. At the D21S11 locus, the use of primers 1 and 2 can be combined to identify DNA samples with high temperature treatment
The Role of Host Genetics Regulating Proteins in HIV-1 Susceptibility: Epidemiological and Demographic Insights on HIV-1 in Indonesia (2022) Saefudin, Siti Amalia Firdza; Handayani, Niken Satuti Nur
Indonesian Journal of Tropical and Infectious Disease Vol. 12 No. 3 (2024)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v12i3.54366

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) remains a global public health concern, marking 52,995 cases in Indonesia alone, dominated with CRF01_AE strain which is classified as an X4 strain or a virus that uses CXCR4 co-receptor. This highlights the urgent needs  to develop therapies that utilize CXCR4 inhibitors to modulate HIV-1 infection and replication. The aims of this study were to assess the epidemiological and demographic insights on HIV-1 in Indonesia in 2022, and connecting it to the dominated strain to further assess various host genetics known to promote HIV-1 infection, focusing on the co-receptors CCR5 and CXCR4. A systematic review was conducted, analyzing published studies and the 2022 HIV/AIDS report from the Ministry of Public Health of Indonesia. Additionally, the study evaluated the therapeutic potential of CXCR4 antagonists, including AMD3100, AMD070, BPRCX807, and MCo-CVX-5c, known for their anti-HIV-1 activity. Among the listed antagonists, AMD070 and MCo-CVX⎯5c are advancing among the others, leading to a potential most advanced combination antiretroviral therapy (cART). This research contributed to the development of personalized treatment strategies for HIV-1 by providing insights into the genetic factors influencing co-receptor regulation and HIV-1 susceptibility.
Co-Authors Andika Tripramudya Onggo Anita Widianawati, Anita Aurantika, Rindu BUDI SETIADI DARYONO Dian Pratiwi, Arini Feri Sukmawati Ganies Riza Aristya Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hery Wijayanto Hidayat, Madyan Akmal Indra Lesmana Karmilah, Karmilah Kazuo Yoshida Kazuo Yoshida, Kazuo Laksono Trisnantoro Latifah, Vida Rahma Maria Natalina Lumban Gaol Mukhlis Mukhlis Nainggolan, Ananto Puradi Nobukazu Tanaka Nobukazu Tanaka, Nobukazu Nugraheni, Kireida Asta Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan P, Purnomo Pertiwi, Ivory Martha Pratiwi, Arini Dian Priyambodo Priyambodo Rarastoeti Pratiwi Rini Widayanti Rini Widayanti Rury Eprilurahman Saefudin, Siti Amalia Firdza Thoifur Ibnu Fajar, Muhammad Tony Febri Qurniawan Trijoko Trijoko (Trijoko) Tuty Arisuryanti, Tuty Widyasari, Adristi Shafa Yusuf, Adib Fakhruddin Yuyun Machwiyah Yuyun Machwiyah