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Esti Harpeni
Staf pengajar jurusan Budidaya Perairan Unila
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences Other

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Aplikasi Ekstrak Daun Ketapang (Terminalia catappa L.) terhadap Udang Vaname (Litopenaeus vannamei) yang Terinfeksi Penyakit White Feces Disease (WFD) Helda, Yeni; Harpeni, Esti; Supono, Supono
Jurnal Masyarakat Akuakultur Indonesia Vol 2, No 2 (2018)
Publisher : Masyarakat Akuakultur Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.538 KB)

Abstract

Udang vaname merupakan komoditas unggulan di bidang perikanan memiliki nilai ekonomis yang tinggi. Permasalahan yang timbul dari kegiatan budidaya udang yaitu serangan penyakit. Jenis penyakit yang sedang marak adalah adalah White Feces Disease (WFD). Secara umum udang yang terserang penyakit WFD mengalami pertumbuhan yang abnormal dan kematian. Timbulnya penyakit sering dikaitkan oleh keberadaan bakteri Vibrio spp. serta faktor lingkungan yang buruk. Salah satu upaya yang dapat dilakukan untuk penanggulan penyakit ini yaitu penggunaan ekstrak daun ketapang (Terminalia catappa.L) dengan cara perendaman. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dan 3 ulangan yaitu kontrol negatif, kontrol positif, dan ekstrak daun ketapang dengan berbagai konsentrasi 10, 20, 30%. Tujuan penelitian ini mengkaji pengaruh pemberian ekstrak daun ketapang sebagai bahan alami yang mampu digunakan untuk penanggulangan penyakit WFD pada udang vaname. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun ketapang sebanyak 30% dengan metode perendaman mampu meningkatkan nilai SR (Suvival Rate), serta adanya penurunan total koloni bakteri Vibrio dibandingkan tanpa pemberian ekstrak daun ketapang.
THE EFFECT OF IMMERSION OF MANGROVE Avicennia alba (Tomlinson, 1986) LEAF EXTRACT WITH DIFFERENT CONCENTRATIONS IN PREVENTING BACTERIAL DISEASE Vibrio harveyi (Johnson & Shunk, 1936) IN VANAME SHRIMP (Litopenaeus vannamei) (Boone, 1931) Farida, Eka Nur; Diantari, Rara; Harpeni, Esti; Wardiyanto, Wardiyanto; Hasani, Qadar; Yulianto, Herman; Yusuf, Maulid Wahid; Susanti, Oktora
e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Vol 8, No 2 (2020)
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.304 KB) | DOI: 10.23960/jrtbp.v8i2.p1001-1008

Abstract

Vibriosis is a disease that often attack shrimp culture, some of the most dangerous types of Vibrio bacteria are V. harveyi. The use of natural materials is an alternative that can be applied to prevent bacterial disease. One of the mangrove species that has an antibacterial compound is A. alba. A. alba has alkaloid, saponins, and flavonoids compounds that can inhibit the function of cytoplasmic membranes and energy metabolism in bacteria. This study was conducted to determine the effectiveness of A. alba leaf extract A. alba in inhibiting V. harveyi disease in vaname shrimp (Litopenaeus vannamei). This research was conducted in several stages, namely extraction of mangrove leaves, both in vivo and in vitro. The highest SR, RPS and MTD values were obtamed at a concentration of 250 mg/l with each value of 80 %, 60 %, 75th respectively hour. Mangrove leaf extract A. alba was able to prevent diseases caused by the bacteria V. harveyi, with the best results at an extract concentration of 250 mg/l where the concentration can significantly higher SR, RPS, and MTD compared to other treatments.
PENGARUH INTENSITAS CAHAYA TERHADAP PIGMEN CAROTENOID, FUCOXANTHIN, DAN PHAEOPHYTIN ZOOXANTHELLAE DARI ISOLAT KARANG LUNAK Zoanthus sp. Fitriyani, Weni; Harpeni, Esti; Muhaemin, Moh.
Maspari Journal : Marine Science Research Vol 9, No 2 (2017): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (779.933 KB) | DOI: 10.36706/maspari.v9i2.4480

Abstract

Zooxanthellae merupakan simbion karang yang membantu pertumbuhan dan pembentukan warna karang. Warna karang dihasilkan dari pigmen yang dimilikinya, seperti carotenoid, fucoxanthin dan phaeophytin. Ketiga pigmen tersebut juga berfungsi dalam menyerap cahaya tidak mampu diserap oleh klorofil, yang kemudian akan ditransfer ke pusat fotosintesis. Tujuan penelitian yaitu untuk mengetahui pengaruh intensitas cahaya terhadap pigmen carotenoid, fucoxanthin dan phaeophytin zooxanthellae dari isolat karang lunak Zoanthus sp. Penelitian dilaksanakan pada bulan Juli - Agustus 2016, bertempat di Laboratorium Budidaya Perikanan Jurusan Perikanan dan Kelautan Fakultas Pertanian Universitas Lampung. Penelitian ini dilakukan dengan empat intensitas cahaya yang berbeda yaitu IC1=3800 Lux, IC2=6250 Lux, IC3=7980 Lux dan IC4=11800 Lux. Berdasarkan uji analisis ragam pada selang kepercayaan 95% menunjukkan bahwa intensitas cahaya berpengaruh terhadap konsentrasi carotenoid dan fucoxanthin, namun intensitas cahaya tidak berpengaruh terhadap konsentrasi phaeophytin. Intensitas cahaya yang paling baik digunakan untuk produksi carotenoid, fucoxanthin dan phaeophytin yaitu IC3 (7980 Lux).KATA KUNCI: Cahaya, carotenoid, fucoxanthin, phaeophytin, zooxanthellae.
KAJIAN Ulva sp. SEBAGAI SUPLEMEN PAKAN TERHADAP PERFORMA PERTUMBUHAN DAN RESPON IMUN NON-SPESIFIK IKAN NILA (Oreochromis niloticus) Harpeni, Esti; Santoso, Limin; Sari, Winda Rohaila; Purba, Duma Oktorina
Maspari Journal : Marine Science Research Vol 7, No 2 (2015): Edisi Juli
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/maspari.v7i2.2486

Abstract

Peningkatan produksi melalui intensifikasi sistem budidaya menyebabkan peningkatan penggunaan pakan buatan. Pakan buatan yang baik harus memiliki kecukupan nutrisi bagi pertumbuhan optimum ikan selain juga meningkatkan resistensi terhadap penyakit. Penggunaan suplemen pakan menjadi penting dilakukan untuk memperoleh panen yang maksimal. Penggunaan algae sebagai suplemen pakan telah diketahui cukup potensial sebagai sumber protein dan metabolit sekunder melawan patogen. Namun, pengaruh pakan dengan penambahan algae terhadap pertumbuhan dan ketahanan tubuh ikan mungkin bervariasi tergantung pada jenis algae dan ikannya. Oleh karena itu, penelitian ini dilakukan untuk mengkaji pengaruh pemberian suplemen paka Ulva sp. terhadap performa pertumbuhan dan respon imun non-spesifik ikan nila. Penelitian dilakukan dengan perlakuan 5 perlakuan dan 3 ulangan, A: Suplementasi Ulva sp. 0% pakan; B: 4% pakan; C: 8% pakan; D: 12 % pakan; dan E: 16% pakan. Hasil penelitian ini menunjukkan adanya peningkatan performa pertumbuhan dan respon imun non spesifik. Perubahan level jaringan ke level berat terjadi seiring dengan peningkatan dosis suplementasi Ulva sp. pada pakan. Suplementasi dengan dosis 4% pakan merupakan perlakuan terbaik.KATA KUNCI: Nila, pertumbuhan, respon imun non-spesifik, suplementasi, Ulva sp.
PENGARUH INTENSITAS CAHAYA TERHADAP KANDUNGAN KLOROFIL-a dan -c ZOOXANTHELLAE DARI ISOLAT KARANG LUNAK Zoanthus sp. Sayekti, Sundari; Harpeni, Esti; Muhaemin, Moh.
Maspari Journal : Marine Science Research Vol 9, No 1 (2017): Edisi Januari
Publisher : UNIVERSITAS SRIWIJAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.062 KB) | DOI: 10.36706/maspari.v9i1.4227

Abstract

Terumbu karang sangat sensitif terhadap perubahan alam, salah satunya seperti peningkatan intensitas cahaya. Intensitas cahaya akan mempengaruhi pigmen klorofil-a dan –c alga simbion zooxanthellae yang hidup di dalam jaringan karang, sebab intensitas cahaya memiliki peranan penting dalam proses fotosintesis pada zooxanthellae. Penelitian bertujuan untuk mengetahui pengaruh intensitas cahaya terhadap kandungan klorofil -a dan -c pada zooxanthellae. Penelitian dilaksanakan pada bulan Juli-Agustus 2016, bertempat di Laboratorium Budidaya Perikanan, Program Studi Budidaya Perairan Fakultas Pertanian, Universitas Lampung selama 66 jam. Penelitian menggunakan 4perlakuan dan 5 ulangan yaitu perlakuan intensitas cahaya 3800 lux (IC1), 6250 lux (IC2), 7980 lux (IC3), dan 11800 lux (IC4) dan sampel karang yang digunakan yaitu karang lunak Zoanthus sp. dari Perairan Teluk Lampung. Parameter utama yang diamati adalahkandungan klorofil-a dan -c pada zooxanthellae dengan menggunakan spektrofotometer dan parameter pendukung yaitu kualitas air. Data klorofil-a dan -c dianalisis dengan analisis ANOVA dan dilanjutkan dengan uji BNT. Hasil penelitian menunjukkan bahwapemberian intensitas cahaya yang berbeda memberikan pengaruh terhadap kandungan klorofil-a pada zooxanthellae, tetapi tidak memberikan pengaruh terhadap kandungan klorofil-c pada zooxanthellae.KATA KUNCI: Intensitas cahaya, klorofil-a, klorofil-c, Zoanthus sp., zooxanthellae.
BAKTERI Bacillus coagulans SEBAGAI FEED PROBIOTIC UNTUK MENINGKATKAN PERFORMA UDANG JERBUNG Fenneropenaeus merguensis (de Man, 1888) Supono, Supono; Taqiyatin, Hani; Harpeni, Esti
Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Vol 18 No 1 (2020): Jurnal Agroqua
Publisher : University of Prof. Dr. Hazairin, SH

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.621 KB) | DOI: 10.32663/ja.v18i1.1216

Abstract

Banana shrimp (Fenneropenaeus merguensis) is an alternative species of local Indonesian shrimp that has the opportunity to be commercially cultivated. One important factor that influences shrimp growth is the feed. Improving the quality of shrimp feed can be done by adding probiotics, one of which is by using Bacillus coagulans. The purpose of this research was to study the effect of B. coagulans bacterium on feed on the growth, feed conversion ratio, and survival rate of Jerbung shrimp. This research was carried out for 35 days. The study design used was a completely randomized design consisting of 4 treatments and 3 replications. The treatment was in the form of feed mixed with B. coagulans with a dose of 0 ml/kg of feed (control), 10ml×106CFU/kg of feed, 20ml×106CFU/kg of feed, and 30ml×106CFU/kg of feed. The results showed that the administration of B. coagulans mixed in the feed significantly affected the growth of weight, length, daily growth rate, feed conversion ratio, and protein efficiency ratio, not significantly different from the survival rate of the jerbung shrimp. The best treatment occurred in the treatment of B. coagulans 10ml×106 CFU/kg of feed.
Non-Specific Immune Response and Growth Performance of Clarias gariepinus (Burchell, 1822) Feeded with Moringa oleifera Leaf Flour Suplementation (Lamk, 1785) Nainggolan, Tio Naomi; Harpeni, Esti; Santoso, Limin
Jurnal Perikanan dan Kelautan Vol 26, No 2 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.2.102-114

Abstract

Sangkuriang catfish (Clarias gariepinus) is a cultivated species favored by Indonesian and has great potential for development. However, the obstacle to the success of catfish cultivation is a disease. One alternative to overcome this problem is by giving immunostimulants from natural ingredients. The purpose of this study was to test the effectiveness of feeding with moringa leaf powder on non-specific immunity and growth performance of sangkuriang catfish. The study used a completely randomized design (CRD) with 4 treatments (100% commercial feed, commercial feed with 1.5% Moringa leaf supplementation, commercial feed with 3% Moringa leaf supplementation, and commercial feed with Moringa leaf supplementation 4.5%, and 3 replications. The results showed that the commercial feed with 4.5% moringa leaf supplementation gave the best results for total erythrocytes of 5.97x104 cells/mm3 and MCV of 92.10 fl, and increased the absolute weight growth of 46.42 g. However, it did not show a significant effect on total erythrocytes, hemoglobin, hematocrit, MCHC, MCH, MCV, total leukocytes, leukocyte differentials, phagocytic activity, phagocytosis index, absolute weight growth survival rate, and the value of the feed convention ratio (FCR).
Isolation and Screening of Amylolytic and Proteolytic Thermophilic Bacteria from Way Panas Hot Spring, Kalianda, South Lampung Mahestri, Laras; Harpeni, Esti; Setyawan, Agus
Jurnal Perikanan dan Kelautan Vol 26, No 3 (2021)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.3.161-168

Abstract

Amylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereusAmylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereus
Non-Specific Immune Response and Growth Performance of Clarias gariepinus (Burchell, 1822) Feeded with Moringa oleifera Leaf Flour Suplementation (Lamk, 1785) Nainggolan, Tio Naomi; Harpeni, Esti; Santoso, Limin
Jurnal Perikanan dan Kelautan Vol. 26 No. 2 (2021): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.2.102-114

Abstract

Sangkuriang catfish (Clarias gariepinus) is a cultivated species favored by Indonesian and has great potential for development. However, the obstacle to the success of catfish cultivation is a disease. One alternative to overcome this problem is by giving immunostimulants from natural ingredients. The purpose of this study was to test the effectiveness of feeding with moringa leaf powder on non-specific immunity and growth performance of sangkuriang catfish. The study used a completely randomized design (CRD) with 4 treatments (100% commercial feed, commercial feed with 1.5% Moringa leaf supplementation, commercial feed with 3% Moringa leaf supplementation, and commercial feed with Moringa leaf supplementation 4.5%, and 3 replications. The results showed that the commercial feed with 4.5% moringa leaf supplementation gave the best results for total erythrocytes of 5.97x104 cells/mm3 and MCV of 92.10 fl, and increased the absolute weight growth of 46.42 g. However, it did not show a significant effect on total erythrocytes, hemoglobin, hematocrit, MCHC, MCH, MCV, total leukocytes, leukocyte differentials, phagocytic activity, phagocytosis index, absolute weight growth survival rate, and the value of the feed convention ratio (FCR)
Isolation and Screening of Amylolytic and Proteolytic Thermophilic Bacteria from Way Panas Hot Spring, Kalianda, South Lampung Mahestri, Laras; Harpeni, Esti; Setyawan, Agus
Jurnal Perikanan dan Kelautan Vol. 26 No. 3 (2021): October
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.26.3.161-168

Abstract

Amylolytic and proteolitic activity from thermophilic bacteria have been observed from the Way Panas hot springs, Kalianda, South Lampung. This study aims to isolate and screen amylase and protease enzyme-producing thermophilic bacteria and their potential in fisheries. A total of 8 bacterial isolates were examined from the Way Panas hot spring, Kalianda. Each was cultured on selective medium for amylase and protease production. The results showed that 4 bacterial isolates were able to hydrolyze starch (A.WP.50.1, A.WP.50.1, A.WP.50.3, and A.WP.50.4) were able hydrolyze starch and no bacterial isolates had hydrolysis activity against protein. The highest activity of amylolitic was achieved by A.WP.50.4 bacteria isolate. Based on the phenotype, biochemistry assay, and 16s rDNA sequencing, the A.WP.50.4 bacteria isolate have high similarity and identified as Bacillus cereus.
Co-Authors A.A. Ketut Agung Cahyawan W Agus Setyawan Ari Widodo Berta Putri Dwinitasari, Nandya eko efendi Ely Novisa Farida, Eka Nur Febriyani, Uun Fitriyani, Weni Helda, Yeni Herman Yulianto Karima, Nisa Limin Santoso Mahestri, Laras Melisha, Melisha Moh. Muhaemin Muamar, Aslam Nainggolan, Tio Naomi Oktafiani, Melinda Permata Sari, Kurnia Dwi Prabarini, Dentiana Purba, Duma Oktorina Qadar Hasani Rara Diantari Sari, Winda Rohaila Sasty Osuna Sitompul Sayekti, Sundari Sonida, Aulia Supono Supono Susanti, Oktora Taqiyatin, Hani Tarsim . Tarsim Tarsim Tutik Kadarini Wardiyanto - Wardiyanto Wardiyanto, Wardiyanto Wijayanti, Arlin Yayu Saskia Yolanda, Laksmita Yusup, Maulid Wahid