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All Journal Jurnal Ilmu Dasar Jurnal Kedokteran Brawijaya Majalah Kedokteran Bandung Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Urology Journal of Agromedicine and Medical Sciences Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Medika Kartika : Jurnal Kedokteran dan Kesehatan Indian Journal of Forensic Medicine & Toxicology JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Dina Helianti
Department Of Histology, Faculty Of Medicine, University Of Jember, Jember, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Shallot (Allium cepa L.) Peel Infusion Ameliorates Kidney Histopathological Damages in Diazinon-Induced Wistar Rats Bi’izzyk, Avie Baldana; Helianti, Dina; Wahyudi, Septa Surya; Dewi, Rosita
Majalah Kedokteran Bandung Vol 56, No 1 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3307

Abstract

Diazinon, an organophosphate pesticide, is used extensively in agricultural sector. Consumption of agricultural products containing diazinon residue may lead to harmful health consequences. Among these is nephrotoxicity, which includes lipid peroxidation, that can damage the kidney. Flavonoids in shallot peel can scavenge free radicals, inhibit necrosis, and activate bone marrow-derived cells for cell regeneration. This study aimed to determine the correlation between shallot peel infusion (SPI) dose and kidney damage amelioration to establish the maximum effective dose of SPI to ameliorate kidney histopathological damage in diazinon-induced rats. This study was performed at the Pharmacology Laboratory, Faculty of Medicine, University of Jember, Indonesia, in April 2021, on 35 rats that were divided into 7 groups—normal, diazinon, and five treatment groups. Diazinon 40 mg/kgBW was administered on day 1-7, while SPI 125, 250, 500, 1,000, 2,000 mg/kgBW were administered on day 8–14 according to the treatment group. Kidney histopathological slides with hematoxylin-eosin (H.E.) staining were assessed using Kocoglu scoring and Kidney damage scores of the treatment groups were analyzed using Pearson test. The maximum effective dose was determined using regression test. The damages found in diazinon-induced rats were tubular degeneration, necrosis, and inflammation with a higher damage score than normal rats (p<0.05). Pearson test showed moderate correlation (coefficient -0.594). Higher SPI doses presented lower kidney damage scores, with 1,359 mg/kgBW being the maximum effective dose. SPI dose and the kidney damage amelioration are moderately correlated with a SPI maximum effective dose to ameliorate kidney damage in diazinon-induced rats of 1,359 mg/kgBW.
INFUSA KULIT BAWANG MERAH (Allium cepa L.) MEMPERBAIKI KERUSAKAN HATI TIKUS WISTAR YANG DIINDUKSI DIAZINON Aini, Nada Qurrota; Dewi, Rosita; Pralampita, Pulong Wijang; Helianti, Dina
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 6 No 4 (2023): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penggunaan diazinon dalam membasmi hama tanaman akan meninggalkan residu pada tanaman sehingga jika dikonsumsi memberikan dampak buruk. Metabolisme diazinon di hati meningkatkan jumlah radikal bebas dan menurunkan aktivitas enzim antioksidan yang akan mengakibatkan kerusakan sel hati. Kulit bawang merah (Allium cepa L.) mengandung kuersetin yang dapat memperbaiki kerusakan jaringan akibat stres oksidatif. Penelitian ini bertujuan mengetahui hubungan antara dosis infusa kulit bawang merah (IKBM) dan tingkat kerusakan hati tikus yang diinduksi diazinon dan menentukan dosis efektif maksimum IKBM. Tikus dibagi menjadi kelompok normal, diazinon, dan IKBM. Diazinon diberikan dengan dosis 40 mg/kgBB selama 7 hari dilanjutkan dengan IKBM dengan dosis 125, 250, 500, 1.000, dan 2.000 mg/kgBB selama 7 hari. Pengamatan mikroskopik dilakukan terhadap 100 sel hati dengan pewarnaan hematoxylin-eosin (HE) berdasarkan skor Manja Roenigk. Tingkat kerusakan hati antara dua kelompok dianalisis menggunakan uji Mann-Whitney, sedangkan korelasi antara dosis IKBM dan tingkat kerusakan hati, dan dosis efektif maksimum IKBM ditentukan dengan uji regresi. Rerata skor histopatologi hati tertinggi didapatkan pada kelompok diazinon dan terendah pada kelompok normal (p<0,05). Uji regresi menunjukkan terdapat hubungan eksponensial antara dosis IKBM dan tingkat kerusakan hati (p<0,05), yaitu semakin tinggi dosis IKBM, semakin rendah skor histopatologi hati dan dosis efektif maksimum IKBM adalah 1.347,28 mg/kgBB. Sebagai kesimpulan, semakin tinggi dosis IKBM, semakin rendah tingkat kerusakan hati tikus yang diinduksi diazinon. Pemberian IKBM melebihi dosis 1.347,28 mg/kgBB akan meningkatkan kerusakan hati. Kata kunci: bawang merah, diazinon, hati, histopatologi DOI : 10.35990/mk.v6n4.p329-339
Potential of shallot peels as a daily antioxidant supplement against cigarette smoke-induced lung damage Helianti, Dina; Dewi, Rosita; Munawaroh, Ayu; Rachmania, Sheilla; Maulana, Aditha Satria; Abrori, Cholis; Sumadi
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 15, No 3, (2024)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol15.Iss3.art10

Abstract

Background: Cigarette smoking damages the alveoli through oxidative stress. Shallot peels containing flavonoids, especially quercetin, potentially serve as a daily antioxidant supplement to impede lung tissue damage induced by cigarette smoke. However, the maximum effective dose is yet to be determined. Objective: This research was designed to establish the maximum effective dose of shallot peel infusion (SPI) to prevent oxidative stress and histopathological lung damage induced by cigarette smoke. Methods: This experimental laboratory was a posttest-only control group design. A total of 24 male Rattus norvegicus Wistar strain, were allocated into 6 groups: a control group and 5 SPI-treated groups. All rats were exposed to 2 cigarettes/day and were treated for 28 days with aquabidest and different doses of SPI (0 mg/kgBW, 125 mg/kgBW, 250 mg/kgBW, 500 mg/kgBW, 1,000 mg/kgBW, and 2,000 mg/kgBW). The level of oxidative stress in serum was measured malondialdehyde (MDA) level with ELISA, and histopathological lung damage was estimated using the lung histopathological damage scoring method assessing inflammatory cells, alveolus lumen and inter-alveoli junction.Results: The quadratic regression analysis revealed the maximum effective dose of SPI to prevent oxidative stress and lung damage were 1,435 mg/kgBW and 1,206 mg/kgBW, respectively. In the histopathological examination of the lungs, the administration of SPI up to a dose of 1206 mg/kg BW prevents the inflammatory process caused by cigarette smoke, which is indicated by the number of inflammatory cells, the thickness of the alveolar septum, and the increasingly normal shape of the alveolar lumen.Conclusion: SPI doses of less than 1,206 mg/kgBW are safe and effective daily antioxidant supplements in rats exposed to cigarette smoke and have the potential to be further studied for application in humans.
Liver Histopathological Analysis in The Acute Toxicity Test of Shallot (Allium cepa L.) Peel Extract in Rats (Rattus norvegicus) Aprilya Pratiwi, Nadilla; Dewi, Rosita; Agustina, Dini; Helianti, Dina; Rachmania, Sheilla
Journal of Agromedicine and Medical Sciences Vol. 11 No. 3 (2025)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v11i3.53736

Abstract

Shallot (Allium cepa L.) contains bioactive compounds with antioxidant effects. In addition to the tuber, the shallot’s peel is a rich source of flavonoids with demonstrated capacity to mitigate oxidative stress. Prior studies have evaluated the antioxidant efficacy of shallot peel in ameliorating organ damage and have established its maximum effective dose. The subsequent step required for its potential therapeutic application is toxicity test. This study aimed to analyze the liver histopathological changes in the acute toxicity test of shallot peel extract (SPE) in rats based on OECD TG 420. This research was an experimental with a post-test-only control group design. Five female rats were used as the control group administered dimethyl sulfoxide (DMSO), while 5 female rats were used in the treatment group administered SPE at 5,000 mg/kg body weight (BW) (one of them had been used for a preliminary test with the same dosage). On day 15, a necropsy was conducted, followed by histopathological observation of the hematoxylin-eosin (HE)-stained liver histopathological slide. The damage to hepatocytes was evaluated using Manja Roenigk criteria. The average histopathological score per liver cell of the control group was 1.226 ± 0.0065 and the treatment group was 1.235±0.0079. The Mann-Whitney test showed that the liver histopathological score of the treatment group was not different from that of the control group (p>0.05). It can be concluded that SPE 5,000 mg/kg does not show acute toxic effects in rats, with LD₅₀ estimated at >5,000 mg/kg according to OECD standards.
Acute Toxicity Test of Shallot (Allium cepa L.) Peel Extract on Ovarian Follicles Count in Rats (Rattus norvegicus) Helianti, Dina
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol. 22 No. 2 (2025): Jurnal Ilmu Farmasi dan Farmasi Klinis
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/jiffk.v22i2.11529

Abstract

Shallot skin (Allium cepa L.) is a natural material with potential as a traditional medicine due to its high content of the antioxidant quercetin. However, excessive consumption of shallot skin may cause the antioxidant properties of quercetin to shift to pro-oxidant effects via auto-oxidation and metal-binding reactions. Uncontrolled pro-oxidants induce oxidative stress that damages body cells, including ovarian follicle cells. This acute toxicity study aimed to evaluate the toxic effects of shallot skin extract on the ovary using ovarian follicle count as an indicator. This study employed a true experimental design with a posttest-only control group. The method referred to the OECD 420 guideline (fixed dose procedure) using rats (Rattus norvegicus) as test animals. The results showed no significant difference in the mean follicle counts between the control and treatment groups across all follicle categories: primary follicles (p = 0.278), secondary follicles (p = 0.452), DeGraaf follicles (p = 0.39), corpus luteum (p = 0.752), atretic follicles (p = 1.0), and total follicles (p = 0.60). Thus, follicle counts in all categories did not differ significantly (p>0.05). It can be concluded that shallot skin extract does not exert toxic effects on ovarian follicle numbers in rats.
Integrating Extremely Low Frequency Magnetic Field Studies in Science Education: “A Case Study on 500 kV Extra High Voltage Transmission Lines" Sudarti Sudarti; Sumardi Sumardi; Singgih Bektiarso; Wahyu Muldayani; Dina Helianti; Ayu Munawaroh Aziz; Aditya Kurniawan; Rio Dermawan; Tania Ardiani; Rista Setiani
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9948

Abstract

Introduction: Until now, the impact of Extremely Low Frequency electromagnetic field radiation (ELF-EMF) by extra high voltage transmission lines (500 kV-EHVTL) is still debated. While WHO recommends a threshold of magnetic field exposure of 100 µT for up to 24 hours/day is declared safe for health. This study aims to examine the Integration of Extremely Low Frequency Magnetic Field Studies in Science Education: "Case Study on 500 kV-EHVTL". This study is important to support literacy in Environmental Physics and Environmental Radiation courses. The location of the study is a 500 kV EHVTL building located in Gondang Wetan District, Pasuruan Regency, East Java, Indonesia. Measurement of ELF magnetic field intensity using the EMF Tester-827 tool. The measurement position is at point 0 (directly below), and lateral distances of 50m, 100m, 150m, 200m, and 250m from the EHVTL power grid cable, as well as distances of 0m, 1m, 5m, 10m, and 15m from the EHVTL power grid tower. The intensity of ELF magnetic field exposure just below the EHVTL reached a value of 5.73 µT-10,085 µT (increased 170-234 times) compared to the natural magnetic field. While several research results prove that exposure to magnetic fields with an intensity of around 10 µT in vitro causes an increase in cell proliferation. Increased cell proliferation in the human body can have negative effects on health. Based on the research results it was concluded that an increase in the intensity of ELF magnetic field exposure up to 170-234 times can cause biological effects at the cellular level and has the potential to cause health impacts on humans. it is advisable to avoid living under the EHVTL network, and the WHO recommendation that the threshold exposure value of 100 µT is declared safe for humans exposed for up to 24 hours/day needs to be reviewed
ACUTE TOXICITY TEST OF RED ONION (Allium Cepa L) SKIN EXTRACT ON KIDNEY HISTOPATHOLOGY OF RATS (Rattus Norvegicus) Brahmantya Putra, Denny Christian; Helianti, Dina; Wahyudi, Septa Surya; Dewi, Rosita
Indonesian Journal of Urology Vol 33 No 2 (2026)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v33i2.1000

Abstract

Introduction: Shallots (Allium cepa L.) are known to contain quercetin, which is helpful as an antioxidant, but its accumulation in the body can be toxic. Objective: This study aims to determine the effect of administering red onion skin extract on histopathology and weight of the kidney, body weight, and clinical symptoms, as well as determining the LD50. Material & Methods: This research was conducted according to OECD guideline No. 420 Fixed Dose Procedures. Rats were allocated to a control group that was given 3% DMSO and a treatment group that was given red onion peel extract at a dose of 5000 mg/kgBW. Kidney histopathology slides with Hematoxylin-eosin staining were observed by two observers in five fields of view using Fiji ImageJ software and assessed based on the Kocoglu scoring. Kidney histopathology data was analyzed using the Mann-Whitney test, while kidney weight and body weight gain used the Independent Sample T-test. Results: The treatment group experienced significantly higher body weight gain than the control group. On the other hand, observations of the histopathological appearance and weight of the kidney did not show any significant differences. Observation of clinical symptoms showed normal results with no deaths. Conclusion: Administration of 5000 mg/kgBW red onion skin extract in the acute toxicity test had no effect on the histopathology and weight of kidney rats, but had a significant effect on body weight gain. The LD50 of red onion skin extract was found to be >5000 mg/kgBW. Keywords: Red onion skin extract, acute toxicity test, LD50, kidney histopathology.
Co-Authors Aditya Kurniawan Aini, Nada Qurrota Aprilya Pratiwi, Nadilla Ayu Munawaroh Aziz, Ayu Munawaroh Bagus Hermansyah Bintang, Muhammad Farrel Ravidinata Masoga Bi’izzyk, Avie Baldana Brahmantya Putra, Denny Christian Cholis Abrori, Cholis Dini Agustina Erfan Efendi Erna Hartatik Hadi Paramu Hairrudin Hairrudin Hartatik, Erna Khairi, Aji Rayyan Khairi Maulana, Aditha Satria Muhammad Hasan Muhammad Hasan Muhammad Hasan Mukhammad Arif Hadi Khoiruddin Nilam Cahya Nilam Cahya Nindya Shinta Rumastika Pralampita, Pulong Wijang Rahmadhan, Adiz Dwiputra Rio Dermawan Rista Setiani Rondhianto Rondhianto Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi Rosita Dewi S.Pd. M Kes I Ketut Sudiana . septa surya wahyudi, septa surya Shahifa Audy Rahima Sheila Rachmania Sheilla Rachmania, Sheilla Singgih Bektiarso SOETJIPTO Sudarti Sudarti Sumadi Sumardi Sumardi Tania Ardiani Wahyu Muldayani Widjiati Widjiati, Widjiati Yudha Nurdian Yulestrina Widiastuti Yunita Armiyanti