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Biodegradasi Substrat Gergajian Kayu Sengon oleh Jamur Kelompok Pleurotus Asal Bogor Biodegradation of Sengon-wood Sawdust Substrate by Pleurotus Group Fungi from Bogor Elis Nina Herliyana; Dodi Nandika; Achmad Lisdar; I. Sudirman; Arief B. Witarto
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 6, No 2 (2008): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.407 KB) | DOI: 10.51850/jitkt.v6i2.248

Abstract

 White-rot fungi has been started to be developed for enhancing the mushroom based industry in many countries including in environmentally sound of bio-bleaching and bio-pulping technological process. Six isolates of wild Pleurotus group were isolated from various location in Bogor, namely Pleurotus EAB7, EB24, EB14-2, EB6, EA4 and EB9 of which were studied of their ligninolytic character. P. ostreatus HO was used as standard comparison.The ligninolytic character of these six fungi isolates was measured after inoculation into Sengon (Paraserianthes falcataria) wood sawdust substrate inside plastic bag with substrate weight of about 400 gram; other additional substances were paddy scalp, gypsum, calcium and water. Observation was done on Sengon wood sawdust substrate since vegetative phase until reproductive phase. Samples were opened, destructed with Hammer Mill, and then dried with oven on temperature of 40oC in 4 ~ 6 days until water content reach 15%, and weigh about 30 gram for analyses. Analyses were done by measuring water soluble extractive substance (TAPPI T 207 om-88 Standard), NaOH (sodium hydroxide) 1% soluble extractive substance (TAPPI T 212 om-88 Standard), alkohol-benzena soluble extractive substance (TAPPI T 204 om-88 Standard), lignin content (TAPPI T 13 os-54 Standard), holocellulose content with method browning (TAPPI T 211 m Standard), and cellulose content with method Cross and Bevan (Meulenhoff et al. 1977; TAPPI 1996).Having applied of each wild Pleurotus group isolates resulted in increasing of extractives total compounds both in vegetative and reproductive phases. Each wild Pleurotus group isolates shows variation in decreasing average of lignin content (10.7 ~ 89.7%) and cellulose (18.9 ~ 87.4%). Pleurotus EB9 are able to decrease the highest lignin (89.7%) and cellulose (87.4%) content of substrate. Classification based on ligninolytic character is different with classification based on morphological and physiological characters. Pleurotus EB9 seems to be separated from other isolates. This shows that there was different ligninolytic character among the isolates. The best isolate for bio-pulping and bio-bleaching agent is Pleurotus EB9 on vegetative phase.
Utilization of Forestry Waste for Commercial Enoki Mushroom Cultivation Yurico Bakhri; Fatin Hanifah; Anisa Tri Harjanti; Iga Dwi Syahrani; Tiara Antika; Elis Nina Herliyana
Jurnal Silvikultur Tropika Vol 13 No 03 (2022): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.13.03.238-244

Abstract

Jamur enoki memiliki potensi ekonomi yang cukup tinggi dalam pangsa pasar konsumsi terutama untuk masakan Jepang dan Korea. Akan tetapi, di Indonesia belum banyak yang membudidayakan jamur ini karena memerlukan perlakuan suhu yang khusus. Riset ini bertujuan menemukan perlakuan suhu dan komposisi media tanam antara serbuk gergaji kayu sengon dan jerami yang paling efektif untuk pembudidayaan jamur enoki sehingga dapat berkembang pesat di Indonesia. Metode yang digunakan dalam riset ini adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dua faktor, yaitu perlakuan suhu dan media tanam serbuk gergaji kayu sengon dengan jerami. Metode pengambilan data dilakukan secara langsung di laboratorium untuk mengukur lebar pertumbuhan miselium, suhu, kelembaban, dan komposisi media tanam. Pertumbuhan miselium pada komposisi 3:1 memiliki tingkat pertumbuhan yang relatif lebih tinggi pada suhu 13. 9℃ dan kelembaban 96.5%. Kata-kata kunci: Jamur Enoki, serbuk gergaji, jerami, suhu, miselium, perlakuan
SPECIES COMPOSITION, STRUCTURE, AND MANAGEMENT IN GAYO COFFEE-BASED AGROFORESTRY SYSTEM: The Case of Mude Nosar Village, Central Aceh Regency Adisti Permatasari Putri Hartoyo; Labana Hutagalung; Fifit Kulsum; Arzyana Sunkar; Elis Nina Herliyana; Syafitri Hidayati
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 13 No 1 (2023): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.13.1.37-48

Abstract

Gayo coffee has many benefits in terms of the environment, economy, and socio-cultural aspects, especially for the Gayo tribe. Recently, Gayo coffee has been the main commodity for the plant cultivation of the Gayo tribe, including the Mude Nosar as local farmers. One of the ways to cultivate Gayo coffee is through an agroforestry system. However, limited reports on the tree species composition and its management in Gayo coffee-based agroforestry needs to be explored. This study aims i) to identify the tree species composition and structure, as well as 2) to analyze the management of Gayo coffee-based agroforestry system in Mude Nosar Village. The study method used plot establishment for vegetation analysis, and interviewed the Gayo coffee farmers. The results showed that the number of tree species at all growth stages was 26 species. The highest Important Value Index (IVI) at the understory and sapling level in order was rumput kerbau (Paspalum conjugatum) and Gayo coffee (Coffea arabica), while at the seedling, pole, and tree levels were dominated by lamtoro (Leucaena leucocephala). The local farmers often use lamtoro as a shade tree for Gayo coffee in agroforestry system. The species diversity index (H') showed low values at all growth levels, except for understory plants with moderate values. The horizontal structure of the tree stand showed an inverted J curve, meaning that the regeneration rate is normal. The vertical structure of the tree stand belongs to strata C and D. The local farmers have applied good Gayo coffee management practices through an agroforestry system including land and seed preparation, planting, maintenance, and harvesting techniques. Coffee seeds and seedlings are from natural regeneration. Plant maintenance techniques are carried out by weeding, pruning, organic fertilization, and controlling pests and diseases. The harvest coffee season is generally twice a year. The management of Gayo coffee using an agroforestry system impacts the ecological, economic, and social aspects.
White Oyster Mushrooms (Pleurotus ostreatus) Cultivation by Log and Twigs Rubber, Leucaena, Randu, and Balsa Elis Nina Herliyana; Abdul Muhyi
Jurnal Silvikultur Tropika Vol 14 No 01 (2023): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.14.01.81-90

Abstract

Waste high fells form a log and twig wood type Leucaena (Leucaena leucocephala), rubber (Hevea brasiliensis), randu (Ceiba pentandra), and balsa (Ochroma bicolor) can be used as a medium cultivation of Oyster Mushrooms (Pleurotus ostreatus) to increase the value added of these four types of wood. The research aims to analyze the potential for the cultivation of Oyster Mushrooms white on media log and twig four types of wood, the nutrient content of mushrooms, and the appropriateness of business. The reserch used a completely randomized design (CRD) with 9 single factorial on media treatment of logs and twigs of four kindsdifferent wood; and baglog from sawdust as control. The results showed the average wet weight of fruiting bodies on rubber wood and lamtoro (heavy wood) in both the media logs and twigs , reaching 213 grams and is not significantly different from the control (262 grams) , but it is larger than other types of wood cottonwoods and balsa(light wood) which is, reaching only 130 grams. The efficiency of biological media from all kinds of wood twigs higher (29.6-36 %) and significantly different than logs (11-20.5 %). Vegetative phase on media of logs and twigs randu is faster (14 days) than other media and not significantly different. Generative phase (reproductive) kapok’s logs (62 days) and the branches (44 days) is longer and significantly different than other treatments. White Oyster Mushrooms nutritional content of all media types are quite good and worthy of consumption. White oyster mushroom cultivation using media log and twig are fit enough developed. Keywords: heavy wood, light wood, cultivation of white oyster mushroom, nutritional content
Utilization of Lignocellulosic Waste as a Source of Liquid Smoke: A Literature Review, Lampung, Indonesia Santiyo Wibowo; Wasrin Syafii; Gustan Pari; Elis Nina Herliyana
JURNAL KESEHATAN LINGKUNGAN Vol. 15 No. 3 (2023): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v15i3.2023.196-216

Abstract

Introduction: Lignocellulosic biomass waste will become a problem for environmental health if not managed correctly. Biomass waste decomposition can produce methane gas which impacts climate change; it can also be a source of breeding pests and diseases. Various efforts have been made to utilize the waste so that it has an added economic value and is environmentally friendly. One of the waste utilization techniques is the carbonization of lignocellulosic biomass into charcoal and liquid smoke. Discussion: A literature review used the articles from Science Direct, Pubmed, Google Scholar, and Research Gate databases over ten years. This literature review paper aims to provide information on producing and utilizing liquid smoke and concludes with some suggestions on production and application. The literature review results show evidence that liquid smoke has been used since the time of the Neanderthals. Then, since the early nineteen century, liquid smoke has been applied in agriculture as plant growth, soil treatment, pesticide, antimicrobial, rubber coagulant/deodorizer, and antioxidant. Conclusion: A significant contribution of liquid smoke utilization is all lignocellulosic biomass waste that can be carbonized into charcoal and liquid smoke so that it can reduce waste problems. Therefore, liquid smoke can reduce the use of pesticides, herbicides, and insect repellents made from synthetic chemicals that can poison the environment. Not much research has been produced and utilized liquid smoke in the pyrolysis temperature stratification technique. So, there are still quite a lot of research opportunities for the diversification of liquid smoke production.
Pengenalan dan Pengembangan Produk Dari Jamur Liar Edible Di Sukabumi, Jawa Barat Ivan Permana Putra; Sri Listiyowati; Elis Nina Herliyana; Muhammad Iqbal Nurulhaq
Jurnal Pengabdian Masyarakat Bangsa Vol. 1 No. 8 (2023): Oktober
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v1i8.388

Abstract

Berdasarkan hasil observasi awal melalui komunikasi kami dengan penduduk usia produktif sekitar kawasan Goalpara, Sukabumi, Jawa Barat, Desa ini merupakan salah satu lokasi yang memiliki pengetahuan lokal mengenai koleksi, preservasi, dan konsumsi jamur liar edible (JLE). Namun pengetahuan tersebut hanya dimiliki oleh sebagian kecil masyarakat yang berada di sana sehingga perlu untuk diwariskan ke generasi berikutnya. JLE sangat berpotensi untuk digunakan sebagai salah satu alternatif pemenuhan kebutuhan pangan karena jumlahnya banyak, mudah ditemukan, dan telah dilaporkan memiliki berbagai macam manfaat dan nutrisi yang baik. Selain itu, Kelompok perambah JLE di desa tersebut belum memiliki keterampilan untuk mengolah JLE menjadi produk turunannya untuk memberikan nilai tambah serta menyimpannya dalam jangka waktu tertentu. Tujuan dari kegiatan pengabdian ini adalah untuk mengenalkan keragaman LE kepada mitra dan melakukan pendampingan pembuatan produk dari JLE. Metode yang dilakukan meliputi inventarisasi dan validasi identitas taksonomi JLE, sosialisasi dan diseminasi potensi JLE dan cara merambah jamur yang aman, serta pelatihan sederhana preservasi dan pembuatan olahan JLE. Hasil kegiatan pengabdian menunjukkan sebanyak 3 spesies JLE yang bisa ditemukan di sekitar lokasi pengabdian yaitu: supa jambu (Armillaria nova-zelandiae), jamur kuping liar (Auricularia delicata), dan jamur tiram phoenix (Pleurotus pulmonarius). Pada kegiatan pengabdian ini juga telah dibuat beberapa produk diantaranya adalah: kripik jamur liar, jamur tiram krispi, dan jamur kuping kering. Hasil dari kegiatan pengabdian ini diharapkan mampu menjadi alternatif pemenuhan bahan pangan masyarakat dan penambahan nilai dari JLE.
CULTIVATION OF BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA IN FISHERIES WASTE MEDIA AND ANTAGONISTIC TEST AGAINST Rhizoctonia sp. AND Fusarium sp Ananta Kusuma Amanda; Andrea Ajeng Eirenne Kristianti; Libranika Dwi Miswati; Elis Nina Herliyana; Lutfi Hanafi; Rizki Nugraheni Amaliasuci
JOURNAL OF PEOPLE, FOREST AND ENVIRONMENT Vol 2, No 1 (2022): Mei
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jopfe.v2i1.6002

Abstract

Sustainable forest management as a complete ecosystem has problems that are often detrimental to the economy and ecology, one of which is disease. Some examples of pathogens that attack forestry plants are Rhizoctonia sp. and Fusarium sp. which cause leaf blight. An alternative to control it is using chitinolytic bacteria as biological agents which are cultured in fishery waste media. This research aims to (1) determine the appropriate formulation of fish waste as a growth medium for chitinolytic bacteria and (2) determine the ability of the antagonist test of chitinolytic bacteria in inhibiting the growth of pathogenic fungi causing leaf blight. The steps used consisted of (1) media preparation, (2) bacterial and fungal rejuvenation, (3) bacterial culturing in fishery waste media, (4) Bacillus subtilis and Pseudomonas aeruginosa antagonist tests against the fungus Rhizoctonia sp. and Fusarium sp., and (5) primary data analysis using OpenCFU and SAS applications. The result shows that the best fish waste formulation for growing chitinolytic bacteria was CR:KU:SI (2:1:1) with an average colony of 285 colonies for B. subtilis and 202 colonies for P. aeruginosa. The results of Anova analysis showed that the media formulation treatment has a significat effect on the growth of B. subtilis and P. aeruginosa bacterial colonies. The results of the in vitro antagonist test shows that the growth of Rhizoctonia sp could not be inhibited by the bacterium Pseudomonas aeruginosa. While the results of the antagonist test on Fusarium sp. shows if the P-value obtained is p>0,05, which means the data is not normally distributed or there is no effect of the application of B. subtilis and P. aeruginosa bacteria in inhibiting the growth rate of Fusarium sp.
Isolation and Selection of Potential Isolates of Dark Septate Endophyte Fungi from Pinus merkusii Roots Akhir, Jumadil; Budi, Sri Wilarso; Herliyana, Elis Nina; Surono
Jurnal Silvikultur Tropika Vol 14 No 03 (2023): Journal of Tropical Silviculture
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.14.03.236-241

Abstract

Root isolation activities to obtain dark septate endophytic fungi are currently mostly carried out on agricultural and plantation crops, while less so on forestry crops. Roots are a suitable habitat for the growth of various microorganisms, including dark septate endophytic fungi. The research aims to isolate the roots of Pinus merkusii plants from four locations, test pathogenicity, and test the biomass of sweet caisim mustard seeds. The research used a descriptive method by observing the number of dark septate endophytic fungal isolates obtained and the pathogenicity of these isolates. Meanwhile, sweet ciasim mustard seed biomass from the pathogenicity test results of non-pathogenic isolates was analyzed using a completely randomized design. The results of root isolation from Pinus merkusii stands obtained as many as 25 isolates by isolating 1,160 root segments. Isolation of Pinus merkusii roots from the IPB Dramaga Campus obtained 16 isolates, Dabun Gelang Village, Gayo Lues Regency obtained 2 isolates, Gunung Walat University Forest obtained 2 isolates, and Gunung Halimun Salak National Park obtained 5 isolates. Pathogenicity tests showed that 12 isolates were pathogenic and 13 isolates were non-pathogenic. Biomass analysis of sweet ciasim mustard seeds on non-pathogenic isolates obtained the highest biomass in the control treatment (not inoculated) and the lowest in isolates Apg 23.5 and Hs 14.6b. There is still a lack of isolates obtained from isolation activities in this research, so it is necessary to find an appropriate sterilization method to obtain the maximum number of isolates and have the potential to become non-pathogenic isolates. Keywords: Pinus merkusii, dark septate endophyte, isolation
Penerapan Probabilistic Neural Network pada Klasifikasi Patogen Daun Bibit Jabon Berdasarkan Ciri Morfologi Spora Melly Br Bangun; Yeni Herdiyeni; Elis Nina Herliyana; Rossy Nurhasanah
Bulletin of Computer Science Research Vol. 4 No. 2 (2024): Februari 2024
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/bulletincsr.v4i2.325

Abstract

The aim of this research is to clasify pathogen of Jabon’s leaf seedling based on spora morphological features using Probabilistic Neural Network classifier. Three types of pathogen to be classified are Colletotrichum sp., Curvularia sp., and Fusarium sp.. The methodologies used are data acquisition using optilab camera microscope to obtain microscopic image data , preprocessing (grayscale, median smoothing, thresholding Otsu, region filling, median smoothing and dilate), morphology feature extraction (area, perimeter, area convex, convex perimeter, compactness, solidity, convexity and roundness), Probabilistic Neural Network classification, and evaluation. The basic morphological characteristics consisting of area, perimeter, convex area, convex perimeter, and derived morphological characteristics consisting of compactness, solidity, convexity and roundness. The experimental results of the morphological feature extraction showed that the compactness and roundness characteristics can be used to identify the three types of pathogens because with these characteristics each class of pathogen is separate. Testing for this research was carried out using 150 test data from three classes of objects from the dataset, namely class 1 (Colletotrichum sp.), class 2 (Curvularia sp.), and class 3 (Fusarium sp.). Then the results of pathogen classification using the application of the PNN algorithm in testing this research obtained an average accuracy value of 86.8% with a proportion of training data and test data of 80:20. The results of the PNN classification on 150 test data were that there were 36 data classified into Colletotrichum sp., 44 data classified into Curvularia sp., and 50 data classified into Fusarium sp. Further research could be done with the identification of digital microscopic images without cropping and systems that could clasify a colony image of pathogens clearly.
Pathogenicity of Rhizoctonia solani Achmad; Hadi, Soetrisno; Herliyana, Elis Nina; Setiawan, Agus
Jurnal Manajemen Hutan Tropika Vol. 5 No. 1 (1999)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pathogenicity of Rhizoctonia solani was studied by evaluating the ability of the pathogen to attack several ages of Pinus merkusii and Acacia mangium seedlings. Results showed that R. solani attacked P. merkusii from seed stage up to seven week-old seedlings, while eight week-old ones were free from the pathogen’s attack. On A. mangium, 16 day-old seedlings were uninfected by R. solani, while 12 day-old ones were still attacked by the pathogen. Pathogenesis of R. solani was also studied by evaluating the activities of cellulolytic and pectolytic enzymes produced by the fungi and compared them with the activities of the same enzymes produced by Fusarium oxysporum. Result showed that R. solani’s cellulolytic enzymes activity, reflected by the activity of C1-cellulase, was lower compared with that of F. oxysporum. On the other hand, R. solani was more intensive degrading pectin medium than F. oxysporum, such phenomenon reflected higher activity of pectolytic enzymes activity of R. solani compared with that of F. oxysporum.
Co-Authors Abdul Muhyi ABDUL MUNIF Abdul Munif Abdurachman Syafiih Achmad Achmad . Achmad . Achmad . Achmad ; Achmad Achmad ACHMAD ACHMAD Achmad Lisdar Adisti Permatasari Putri Hartoyo Agus Setiawan Ai Rosah Aisah Akhir, Jumadil ANANG PRANOTO HIDAYAT Ananta Kusuma Amanda Andi Sukendro Andrea Ajeng Eirenne Kristianti Anisa Tri Harjanti Ardiansyah Putra Ariana Ariana Arief B. Witarto Arief Noor Rachmadiyanto Arif Ravi Wibowo Arzyana Sunkar Benyamin Dendang Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Deasy Putri Permatasari Dewi Sukma Dodi Nandika Dwierra Evvyernie Dyah LINGGA NP Erianto Indra Putra Eti Artiningsih Octaviani Fatin Hanifah Felliks Tampinongkol, Felliks Fifit Kulsum Fitri Kurniawati Fuzy Yustika Manik Gustan Pari Hanifah Nuryani Lioe Hayati Minarsih Hayati Minarsih Hidayatullah, Deden I. Sudirman Iga Dwi Syahrani Illa Anggraeni Irfan Jelata, Tedi Irfan Kemal Putra Irma Badarina Iskandar Z Siregar Isroi Isroi Ivan Permana Putra Kultsum, Fifit Kunio Tsunoda Labana Hutagalung Laila Fithri Maryam Libranika Dwi Miswati Liza Sakbani Lufthi Rusniarsyah, Lufthi Lul Godi, Rizal Lutfi Hanafi Melly Br Bangun Mira Febrianti Muhammad Alam Firmansyah Nabawiah, Safira Nifa Hanifa Noor Rachmadiyanto, Arief Nurulhaq, Muhammad Iqbal OSICA ASNO FERINA YURTI Ratna Jamilah Reny Meisetyani Reza Pamunca, Airres Rezeka Amalia Rizki Nugraheni Amaliasuci Rossy Nurhasanah Safira Nabawiah Santiyo Wibowo Sarah Diana Yulianti Shodiq Syifaudin, Ikhwan Silviana Arsyad Soetrisno Hadi Sri Listiyowati Sri Wahyuni, Devi Sri Wilarso Budi Surono Suryo Wiyono Syafitri Hidayati Tiara Antika Tjahja Muhandri Toto Toharmat TW DARMONO DARMONO Wartaka Wartaka ; Wasrin Syafii Yurico Bakhri Yusuf Sudo Hadi