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Pengaruh Serbuk Sengon, Daun Jati, dan Tandan Kosong Kelapa Sawit (TKKS) terhadap Kultivasi Jamur Tiram (Pleurotus spp.) Nina Herliyana, Elis; Lul Godi, Rizal; Rusniarsyah, Lufthi
Journal of Tropical Silviculture Vol. 16 No. 01 (2025): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.16.1.55-61

Abstract

Pleurotus cystidiosus (jamur tiram coklat) dan Pleurotus djamor (jamur tiram merah) merupakan salah satu jenis jamur kayu yang banyak dikonsumsi masyarakat karena memiliki kandungan gizi yang tinggi. Limbah serbuk gergaji kayu sengon, Tandan Kosong Kelapa Sawit (TKKS), dan serasah daun jati menjadi alternatif sebagai media tanam dalam kultivasi jamur tiram. Penelitian ini bertujuan mempelajari pertumbuhan dan produksi jamur tiram (Pleurotus spp.) pada media yang mengandung serbuk gergaji kayu sengon, TKKS, daun jati, dan Metode yang digunakan adalah Rancangan Acak Lengkap Faktorial. Faktor pertama terdiri atas jamur tiram cokelat dan merah, faktor kedua terdiri atas 6 komposisi media. Hasil penelitian menunjukkan formula 2 (serbuk sengon 1860 g + daun jati 300 g) memberikan pengaruh terbaik pada fase vegetatif dengan rata-rata pertumbuhan miselium 0,75 cm/hari. Fase generatif paling baik dalam 2 kali panen diperoleh formula 4 (serbuk gergaji kayu sengon dengan TKKS 1:2) yaitu 9 hari. Total bobot basah tertinggi diperoleh formula 4 yaitu 103,39 g dengan nilai efisiensi biologis 53,28%. Hama dan penyakit yang ditemukan adalah tungau, Collembola, dan Trichoderma sp.
Karakteristik Morfologi dan Fisiologi Cendawan Jakaba BHP01 (Sordariomycetes, Ascomycota) Nina Herliyana, Elis; Irfan Jelata, Tedi; Munif, Abdul; Shodiq Syifaudin, Ikhwan
Journal of Tropical Silviculture Vol. 16 No. 01 (2025): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.16.1.33-40

Abstract

Indonesia memiliki hutan tropis yang luas dengan keanekaragaman sumber daya alam hayati yang tinggi, berupa kekayaan flora, fauna dan mikroba. Cendawan merupakan salah satu kekayaan mikroba hutan tropis dengan keanekaragaman yang tinggi dan manfaat yang sangat besar. Salah satu jenis cendawan yang masih belum dikenal adalah jakaba BHP01. Penelitian ini bertujuan mempelajari morfologi dan pertumbuhan meliputi diameter koloni miselium serta sifat fisiologi dari cendawan jakaba BHP01. Metode penelitian meliputi isolasi, pembuatan media biakan, pemurnian, peremajaan, pemeliharaan, pengamatan dan pengujian, uji fisiologi dengan menggunakan media spesifik serta kegiatan identifikasi. Hasil penelitian menunjukan bahwa jakaba BHP01 memiliki laju pertumbuhan yang lambat. Hasil identifikasi dapat diketahui bahwa jakaba BHP01 secara mikroskopik memiliki hifa bersekat, bercabang dan memiliki askospora. Secara makroskopik cendawan ini mempunyai tubuh buah yang berbentuk lonjong berwarna oranye kemerahan dengan ujung berwarna putih. Hasil pengujian aktivitas selulase diperoleh hasil positif. Hasil pengujian ligninolitik menunjukan bahwa jakaba BHP01 termasuk ke dalam cendawan pelapuk putih. Berdasarkan karakteristik tersebut, jakaba BHP01 termasuk ke dalam kelas Sordariomycetes, divisi Ascomycota. Cendawan jakaba BHP01 berpotensi dalam pembuatan pupuk organik dan potensi lainnya, dalam kegiatan pembibitan tanaman pertanian dan kehutanan.
Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD) Hayati MINARSIH; Dyah LINGGA NP; TW DARMONO DARMONO; Elis Nina HERLIYANA
Menara Perkebunan Vol. 79 No. 1: 79 (1), 2011
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v79i1.72

Abstract

AbstractInformation on genetic diversity of Ganoderma spp.causing root rot disease in crops is important to developa proper strategy for the control of Ganoderma disease. Theobjectives of this research were to study the genetic diversityof Ganoderma spp. associated with cacao and its shade trees(Albazia faltacaria, Swietenia mahogani, Adenatheramicrosperma and Leucaena leucocephala) by randomamplified polymorphic DNA (RAPD) analysis. Fourty fivesamples of Ganoderma spp. were used in this research. Theresults showed that DNA amplification using 10 arbitraryoligonucleotide primers produced 220 DNA fragmentsshowing polymorphisms. The cluster analysis showed that 45number of Ganoderma samples had a high variability with thecoefficient value ranged from 0.71 to 0.91. Further analysisusing Winboot software showed that three groups ofGanoderma spp. had a high degree of confidence (>50 %),which were Ganoderma samples from sengon (Paraserianthessp.) of Tasikmalaya, sengon (Paraserianthes sp.) ofPalembang, and mahogany of Jember; whereas the othergroups of samples had a low degree of confidence (<50%).AbstrakInformasi tentang keragaman genetik Ganoderma spp.sebagai penyebab penyakit busuk akar pada tanamanperkebunan sangat diperlukan untuk menerapkan strategiyang tepat dalam upaya perlindungan tanaman perkebunan.Penelitian ini bertujuan untuk mengetahui keragaman genetikGanoderma spp. yang berasosiasi dengan tanaman kakao dantanaman pelindungnya (sengon, mahoni, saga dan lamtoro)dari berbagai wilayah di Indonesia menggunakan penandamolekuler random amplified polymorphic DNA (RAPD).Sebanyak 45 sampel Ganoderma spp digunakan dalampenelitian ini. Amplifikasi DNA dengan 10 primer terpilihmenghasilkan 220 fragmen DNA yang menunjukkan adanyapolimorfisme. Hasil analisis menunjukkan adanya keragamanyang cukup tinggi di antara sampel Ganoderma spp. daripohon inang dan wilayah yang berbeda, dengan nilaikoefisien 0,71-0,91. Berdasarkan analisis bootstrapdiketahui bahwa tiga kelompok sampel Ganoderma spp.memiliki tingkat kepercayaan yang tinggi (>50 %) yaitukelompok Ganoderma spp. yang berasosiasi dengan pohonsengon asal Tasikmalaya, sengon Palembang, dan mahoniJember; sedangkan pengelompokan lainnya menunjukkmenunjukkan tingkat kepercayaan yang rendah (<50 %).
Sprout Test of Seven Types of Sumatran and Javan Rice with the Addition of Liquid Organic Fertilizer Herliyana, Elis Nina; Kultsum, Fifit; Sukma, Dewi; Hidayatullah, Deden; Munif, Abdul
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.787

Abstract

Rice productivity in Indonesia has declined due to a decrease in land area between 2018 and 2019. Another issue with rice seed dormancy is a reduction in productivity. Liquid organic fertilizer (LOF) is one way to overcome seed dormancy. The purpose of this study is to investigate the effect of soaking rice seeds in LOF on germination (viability), normal vigor of growing seeds (vigor), and germination rate. This study took place in the greenhouse and laboratory of the Department of Silviculture, Faculty of Forestry and Environment, IPB, from September to October 2022. Immersion procedures, planting, and data analysis were all steps in monitoring rice germination studies on four types of West Sumatra rice, one variety of West Java rice, and two varieties of rice from the Sukamandi Rice Research Centre. Soaking rice seeds in LOF affects their germination capacity and rate. Pandan Wangi had the maximum germination capacity and average seed count on filter paper medium, while Inpara 8 had the highest germination capacity and average seed count on vermicompost media with a 1:3 immersion. Anak Daro and Cikawasen had the fastest germination rates on filter paper media, whereas Pandan Wangi did the best on vermicompost media. Keywords: Liquid organic fertilizer, local rice, seed dormancy
SPECIES COMPOSITION, STRUCTURE, AND MANAGEMENT IN GAYO COFFEE-BASED AGROFORESTRY SYSTEM: The Case of Mude Nosar Village, Central Aceh Regency Hartoyo, Adisti Permatasari Putri; Hutagalung, Labana; Kulsum, Fifit; Sunkar, Arzyana; Herliyana, Elis Nina; Hidayati, Syafitri
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 13 No 1 (2023): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.13.1.37-48

Abstract

Gayo coffee has many benefits in terms of the environment, economy, and socio-cultural aspects, especially for the Gayo tribe. Recently, Gayo coffee has been the main commodity for the plant cultivation of the Gayo tribe, including the Mude Nosar as local farmers. One of the ways to cultivate Gayo coffee is through an agroforestry system. However, limited reports on the tree species composition and its management in Gayo coffee-based agroforestry needs to be explored. This study aims i) to identify the tree species composition and structure, as well as 2) to analyze the management of Gayo coffee-based agroforestry system in Mude Nosar Village. The study method used plot establishment for vegetation analysis, and interviewed the Gayo coffee farmers. The results showed that the number of tree species at all growth stages was 26 species. The highest Important Value Index (IVI) at the understory and sapling level in order was rumput kerbau (Paspalum conjugatum) and Gayo coffee (Coffea arabica), while at the seedling, pole, and tree levels were dominated by lamtoro (Leucaena leucocephala). The local farmers often use lamtoro as a shade tree for Gayo coffee in agroforestry system. The species diversity index (H') showed low values at all growth levels, except for understory plants with moderate values. The horizontal structure of the tree stand showed an inverted J curve, meaning that the regeneration rate is normal. The vertical structure of the tree stand belongs to strata C and D. The local farmers have applied good Gayo coffee management practices through an agroforestry system including land and seed preparation, planting, maintenance, and harvesting techniques. Coffee seeds and seedlings are from natural regeneration. Plant maintenance techniques are carried out by weeding, pruning, organic fertilization, and controlling pests and diseases. The harvest coffee season is generally twice a year. The management of Gayo coffee using an agroforestry system impacts the ecological, economic, and social aspects.
Potential of Endophytic Bacteria in Controlling The Red Jabon Leaf Spot Pathogens In Vitro Elis Nina Herliyana; Asmoro, Prayogo Probo; Abdul Munif
CELEBES Agricultural Vol. 4 No. 1 (2023): CELEBES Agricultural
Publisher : Faculty of Agriculture, Tompotika Luwuk University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52045/jca.v4i1.416

Abstract

Plant pathogens pose a significant challenge to the cultivation of red Jabon (Anthocephalus macrophyllus) seedlings. Notably, the fungi Rhizoctonia sp. and Pestalotia sp. are responsible for the destructive red leaf spot disease on Jabon leaves. To combat these fungal pathogens, the use of endophytic bacteria has emerged as a potential alternative. Endophytic bacteria can be isolated from various plant sources, although their effectiveness in controlling forest plant pathogens like Jabon has not been thoroughly explored. This study aimed to assess the potential of endophytic bacterial isolates obtained from Pteris ensiformis (Isolates APE15, APE22, APE33, and APE35) in controlling the growth of Rhizoctonia sp. and Pestalotia sp. through in vitro experiments. The antibiosis activity of the endophytic bacteria against the pathogenic fungi was evaluated using the dual culture method on PDA media. The results revealed that among the four endophytic bacterial isolates, APE35 exhibited the highest inhibitory effect on Rhizoctonia sp. (86.79%) and Pestalotia sp. (67.5%), while isolate APE22 only inhibited the growth of Pestalotia sp. (55%). In contrast, isolates APE15 and APE33 were unable to suppress either fungus. The antibiosis activity of APE35 and APE22 resulted in abnormal hyphal growth of the pathogenic fungi, characterized by shriveled, bent, dark-colored, and coiled hyphae. Physiological characterization of the endophytic bacteria revealed their ability to produce protease, cellulase, catalase, and phosphate-dissolving enzymes. Additionally, these bacteria exhibited a Gram-positive nature. This study provides valuable insights into the potential of endophytic bacterial isolates from P. ensiformis (APE35 and APE22) as biological control agents for Rhizoctonia sp. and Pestalotia sp., the causative agents of red Jabon leaf spot disease.
CULTIVATION OF BACILLUS SUBTILIS AND PSEUDOMONAS AERUGINOSA IN FISHERIES WASTE MEDIA AND ANTAGONISTIC TEST AGAINST Rhizoctonia sp. AND Fusarium sp Ananta Kusuma Amanda; Andrea Ajeng Eirenne Kristianti; Libranika Dwi Miswati; Elis Nina Herliyana; Lutfi Hanafi; Rizki Nugraheni Amaliasuci
Journal of People, Forest and Environment Vol. 2 No. 1 (2022): Mei
Publisher : University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jopfe.v2i1.6002

Abstract

Sustainable forest management as a complete ecosystem has problems that are often detrimental to the economy and ecology, one of which is disease. Some examples of pathogens that attack forestry plants are Rhizoctonia sp. and Fusarium sp. which cause leaf blight. An alternative to control it is using chitinolytic bacteria as biological agents which are cultured in fishery waste media. This research aims to (1) determine the appropriate formulation of fish waste as a growth medium for chitinolytic bacteria and (2) determine the ability of the antagonist test of chitinolytic bacteria in inhibiting the growth of pathogenic fungi causing leaf blight. The steps used consisted of (1) media preparation, (2) bacterial and fungal rejuvenation, (3) bacterial culturing in fishery waste media, (4) Bacillus subtilis and Pseudomonas aeruginosa antagonist tests against the fungus Rhizoctonia sp. and Fusarium sp., and (5) primary data analysis using OpenCFU and SAS applications. The result shows that the best fish waste formulation for growing chitinolytic bacteria was CR:KU:SI (2:1:1) with an average colony of 285 colonies for B. subtilis and 202 colonies for P. aeruginosa. The results of Anova analysis showed that the media formulation treatment has a significat effect on the growth of B. subtilis and P. aeruginosa bacterial colonies. The results of the in vitro antagonist test shows that the growth of Rhizoctonia sp could not be inhibited by the bacterium Pseudomonas aeruginosa. While the results of the antagonist test on Fusarium sp. shows if the P-value obtained is p>0,05, which means the data is not normally distributed or there is no effect of the application of B. subtilis and P. aeruginosa bacteria in inhibiting the growth rate of Fusarium sp.
Co-Authors Abdul Muhyi ABDUL MUNIF ABDUL MUNIF Abdul Munif Abdurachman Syafiih Achmad Achmad . Achmad . Achmad . Achmad ; Achmad Achmad ACHMAD ACHMAD Achmad Lisdar Adisti Permatasari Putri Hartoyo Agus Setiawan Ai Rosah Aisah Akhir, Jumadil ANANG PRANOTO HIDAYAT Ananta Kusuma Amanda Andi Sukendro Andrea Ajeng Eirenne Kristianti Anisa Tri Harjanti Ardiansyah Putra Ariana Ariana Arief B. Witarto Arief Noor Rachmadiyanto Arif Ravi Wibowo Arzyana Sunkar Asmoro, Prayogo Probo Benyamin Dendang Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Deasy Putri Permatasari Dewi Sukma Dodi Nandika Dwierra Evvyernie Dyah LINGGA NP Erianto Indra Putra Eti Artiningsih Octaviani Fatin Hanifah Felliks Tampinongkol, Felliks Fitri Kurniawati Fuzy Yustika Manik Gustan Pari Hanifah Nuryani Lioe Hayati Minarsih Hayati Minarsih Hidayati, Syafitri Hidayatullah, Deden Hutagalung, Labana I. Sudirman Iga Dwi Syahrani Illa Anggraeni Irfan Jelata, Tedi Irfan Kemal Putra Irma Badarina Iskandar Z Siregar Isroi Isroi Ivan Permana Putra Kulsum, Fifit Kultsum, Fifit Kunio Tsunoda Laila Fithri Maryam Libranika Dwi Miswati Liza Sakbani Lufthi Rusniarsyah, Lufthi Lul Godi, Rizal Lutfi Hanafi Melly Br Bangun Mira Febrianti Muhammad Alam Firmansyah Nabawiah, Safira Nifa Hanifa Noor Rachmadiyanto, Arief Nurulhaq, Muhammad Iqbal OSICA ASNO FERINA YURTI Ratna Jamilah Reny Meisetyani Reza Pamunca, Airres Rezeka Amalia Rizki Nugraheni Amaliasuci Rossy Nurhasanah Safira Nabawiah Santiyo Wibowo Sarah Diana Yulianti Shodiq Syifaudin, Ikhwan Silviana Arsyad Soetrisno Hadi Sri Listiyowati Sri Wahyuni, Devi Sri Wilarso Budi Surono Suryo Wiyono Tiara Antika Tjahja Muhandri Toto Toharmat TW DARMONO DARMONO Wartaka Wartaka ; Wasrin Syafii Yurico Bakhri Yusuf Sudo Hadi