Luh Gde Sri Surya Heryani
Laboratorium Anatomi Dan Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali, Indonesia

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Karakteristik Struktur dan Morfometri Usus Besar Itik Bali pada Pertumbuhan Fase Starter, Grower, dan Finisher Yulia Khalifatun Nissa; Luh Gde Sri Surya Heryani; I Ketut Suatha; Ni Luh Eka Setiasih; Ni Nyoman Werdi Susari; I Made Sukada
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.035 KB) | DOI: 10.19087/jveteriner.2022.23.3.317

Abstract

Itik bali (Anas sp.) merupakan plasma nutfah asli Indonesia yang dimanfaatkan daging dan telur. Keberadaan itik bali masih kurang mendapat perhatian secara khusus mengenai struktur dan morfometri dasar organ penunjang kehidupan, khususnya usus besar. Seiring masa pertumbuhan, organ itik bali mengalami perkembangan bobot dan struktur. Hal ini yang menyebabkan adanya perbedaan morfometri pada usus besar di umur yang berbeda. Penelitian ini merupakan penelitian observasional non-eksperimental menggunakan itik bali jantan dengan tiga kelompok umur yang mewakili setiap fase, yaitu fase starter (1 bulan), fase grower (3 bulan), dan fase finisher (5 bulan. Hasil pengukuran sekum kiri dan kanan serta kolon itik bali umur 1 bulan meliputi panjang, lebar, dan berat secara berturut-turut yaitu 14,65±0,65 cm, 1,16±0,23 cm, 1,10±0,32 g; 13,70±0,87 cm, 1,01±0,24 cm, 0,96±0,41 g; dan 8,05±0,74 cm, 1,76±0,28 cm, 2,32±0,11 g. Sekum kiri dan kanan serta kolon itik bali berumur 3 bulan berturut-turut yaitu 15,43±1,65cm, 1,05±0,06 cm, 1,83±0,20 g; 13,83±1,59 cm, 1,03±0,10 cm, 1,83±0,20 g; dan 9,08±0,81 cm, 2,15±0,26 cm, 4,66±0,88 g. Sedangkan sekum kiri dan kanan serta kolon itik bali berumur 5 bulan berturut-turut yaitu 18,45±1,34 cm, 1,09±0,96 cm, 2,16±0,32 g; 17,63±1,32 cm, 1,05±0,05 cm, 2,05±0,42 g; dan 10,33±1,50 cm, 2,21±0,23cm, 5,25±0,38 g. Hasil penelitian menunjukkan struktur anatomi dan histologi yang sama, tetapi dalam pengamatan morfometri dapat disimpulkan bahwa perbedaan umur memiliki pengaruh yang nyata terhadap peningkatan morfometri anatomi usus besar itik bali.
STRUKTUR DAN MORFOMETRI LIMPA ITIK BALI (Anas sp.) PADA FASE PERTUMBUHAN Winda Ara Yulisa; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Gede Soma
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.357

Abstract

Limpa merupakan organ yang dikelompokkan ke dalam sistem limfoid sekunder. Limpa memiliki fungsi imunitas terhadap antigen yang masuk ke dalam tubuh dan menghancurkan eritrosit yang rusak. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri limpa itik bali (Anas sp.) pada fase pertumbuhan/grower. Penelitian ini menggunakan 20 ekor itik bali yang dibagi menjadi dua kelompok yaitu jantan dan betina yang masing-masing terdiri atas 10 ekor (umur 2-3 bulan). Hasil data struktur anatomi dan histologi dianalisis secara deskriptif kualitatif, sedangkan data morfometri dan histomorfometri untuk menguji perbedaan antara jantan dan betina digunakan uji Independent sample T-test dengan prosedur analisis menggunakan program SPSS versi 26. Struktur anatomi limpa itik bali berbentuk segitiga piramid dan berwarna cokelat kemerahan, struktur histologi limpa itik bali terdiri atas kapsula, trabekula, pulpa merah dan pulpa putih. Hasil pengukuran morfometri berat limpa itik bali jantan diperoleh 0,68 ± 0,20 g dan berat limpa itik betina 0,66 ± 0,24 g. Volume limpa itik bali jantan dan betina berturut-turut 0,60 ± 0,19 mL dan 0,58 ± 0,23 mL. Hasil pengukuran histomorfometri ketebalan kapsula limpa itik jantan dengan betina adalah 17,97 ± 4,81 ?m; 31,75 ± 6,09 ?m; ketebalan trabekula jantan 17,20 ± 3,26?m ; dan betina 22,54 ± 6,29 ?m; serta diameter pulpa putih jantan adalah 214,69 ± 14,77 ?m; dan diameter pulpa putih betina adalah 199,56 ± 23,58 ?m. Simpulan penelitian ini adalah limpa itik bali jantan dan betina pada fase pertumbuhan/grower memiliki struktur anatomi dan histologi yang sama, morfometri yang tidak berbeda nyata, serta histomorfometri yang menunjukkan perbedaan pada ketebalan kapsula dan trabekula sedangkan diameter pulpa putih tidak jauh berbeda.
Struktur dan Morfometri Ginjal Itik Bali (Anas sp.) pada Fase Pertumbuhan Ni Putu Dewi Setia Sari; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.374

Abstract

Ginjal merupakan organ ekskresi yang berperan dalam membuang zat sisa metabolisme yang tidak dibutuhkan lagi di dalam tubuh. Penelitian ini bertujuan untuk mengetahui struktur dan morfometri ginjal itik bali (Anas sp.) dengan jenis kelamin berbeda pada fase pertumbuhan/ grower. Penelitian menggunakan 32 ekor itik bali yang dibagi menjadi dua kelompok jenis kelamin masing-masing 16 ekor (umur 2-3 bulan). Hasil data struktur dianalisis dengan deskriptif kualitatif,sedangkan data morfometri digunakan uji Independent sample T-test dengan program SPSS. Hasil pengukuran ginjal itik bali jantan diperoleh panjang ginjal kanan 7,256 + 0,388 cm, panjang ginjal kiri 7,175 + 0,437 cm, bobot ginjal kanan 3,50 + 0,61 g, bobot ginjal kiri 3,487 + 0,497 g, volume ginjal kanan 0,350 + 0,103 cm3, volume ginjal kiri 0,362 + 0,088 cm3, glomerulus tipe mamalia 34,025 + 2,931 ?m dengan lebar bowman 8,173 + 2,447 ?m, glomerulus tipe reptil 14,777 + 2,300 ?m dengan lebar ruang bowman 6,676 + 1,780 ?m. Hasil pengukuran ginjal itik bali betina diperoleh panjang ginjal kanan 6,812 + 0,263 cm, panjang ginjal kiri 6,781 + 0,299 cm, bobot ginjal kanan 4,012 + 0,464 g, bobot ginjal kiri 3,987 + 0,401 g, volume ginjal kanan 0,418 + 0,116 cm3, volume ginjal kiri 0,393 + 0,106 cm3, glomerulus tipe mamalia 43,443 + 4,686 ?m dengan lebar bowman 9,068 + 3,483 ?m, glomerulus tipe reptil 23,312 + 2,761 ?m dengan lebar ruang 6,390 + 1,995 ?m. Hasil pengujian terhadap panjang bobotginjal dan ginjal menunjukkan berbeda nyata P<0,05, sedangkan volume ginjal tidak berbeda nyata P>0,05. Hasil pengujian terhadap glomerulus tipe mamalia dan glomerulus tipe reptil menunjukkan berbeda nyata P<0,05. Struktur anatomi danhistologi antara ginjal itik bali jantan dan betina adalah sama, sedangkan morfometri anatomi dan histologi ginjal itik bali jantan dengan betina berbeda.
EVALUATION OF PRESERVED ORGANS OF PLASTINATION WITH THE DEHYDRATION PHASE AT ROOM TEMPERATURE Ni Nyoman Werdi Susari; I Nengah Wandia; I Ketut Suatha; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p26

Abstract

Organ preservation is an effort to maintain the integrity of organs for a long time by preventing further damage and decay processes. Plastination is an organ preservation process by inserting polymer materials to maintain the shape and composition of the organ. This research aims to evaluate the product of a plastination technique carried out using generic chemicals in the community and equipment. The organs used in this plastination process are heart, brain and lungs from the bali cattle. The research materials and tools needed are distilled water, formalin, acetone, liquid silicone rubber, silicon catalyst, 10 ml syringe, plastic bag/tub with lid, vacuum chamber, plastic bucket/tub without lid, and hardening room/box. The vacuum chamber is made from a modified drum so that it can accommodate cadaver plastination (large size). The plastination technique is carried out in four main stages, one of which is the dehydration phase with acetone which is carried out at room temperature. The plastinated organs that are the product of this technique will be evaluated for their flexibility, color, and odor. The results of this research produce plastinated organs that have a stiff texture, pale color, and a non-pungent odor. From the research results obtained, it can be suggested that it needs to be stored in a vacuum for longer (for 1 week) so that the texture can resemble its original shape.  
URINE ANALYSIS OF ETAWA BREED GOATS IN SUKAWATI DISTRICT, GIANYAR REGENCY BALI Gusti Putu Arni Safitri; Anak Agung Sagung Kendran; Luh Gde Sri Surya Heryani; Ida Bagus Komang Ardana; I Nyoman Suarsana; Sri Kayati Widyastuti; Romy Muhammad Dary Mufa
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2024 Vol. 16 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i03.p07

Abstract

Etawa crossbreed goats (Capra aegagrus hircus) are livestock that are starting to be developed among breeders. In goat farming there are health problems that arise both clinically and subclinically, including disorders of the urinary tract. This study aims to determine the clinical condition of Etawa crossbreed goats (PE) in Sukawati District, Gianyar Regency by using urine examination. This study used 30 urine samples from Etawa crossbreed goats at the Raka Etawa Farm. A total of 10-30 ml of urine is taken using the mid-stream method and collected in a urine container which is then tested using a dipstick pad. This research is an observational study with a cross sectional study design using a purposive sampling method. Based on the results of the examination, data was obtained in the form of specific gravity (BJ) with a range of 1,005-1,010 and an average of 1.00567; pH in the range 8-9 and an average of 8.867; a total of 18 samples were found to contain proteins in the range 15-30 and an average of 15.83; and negative results were found in the parameters glucose, negative bilirubin, ketones, erythrocytes, urobilinogen, nitrites and leukocytes. These results indicate that the clinical condition of Etawa crossbreed goats (PE) is healthy (not sick) through urine examination in the form of physical examination and dipstick. It is necessary to carry out additional examinations in the form of examination of urine sidemen and bacterial culture, as well as measuring the specific gravity (BJ) using a refractometer to obtain a more accurate value.
HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE I Kadek Ari Satria Prayoga; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p11

Abstract

Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.
HISTOMORPHOMETRY OF THE DEXTER VENTRICLE OF LOCAL BALI DUCKS AT GROWTH PHASE Delima Marsinta Ida Pasaribu; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; I Ketut Berata; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p20

Abstract

Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.
QUALITY OF DIUTED BOAR SEMEN USING PALMYRA FRUIT WATER WITH THE ADDITION OF VARIOUS CONCENTRATIONS OF EGG YOLK AND MORINGA LEAF EXTRACT I Made Gede Raditya Darmayoga; Wayan Bebas; Luh Gde Sri Surya Heryani; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; I Wayan Sukernayasa
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p30

Abstract

Diluent is a substance used to maintain and protect spermatozoa during the storage process. Natural-based diluents are still rarely found, making it necessary to develop diluents derived from naturally available ingredients. This study aims to evaluate the effect of palmyra fruit water diluent with the addition of various concentrations of egg yolk and moringa leaf extract on the quality of boar semen. This study used one Landrace boar as the semen source, approximately 1.5 years old, in healthy condition. The study employed a Completely Randomized Design CRD with seven treatments: T0 as the control palmyra fruit water diluent only, T1 palmyra fruit water + 10% egg yolk + 5% moringa leaf extract, T2 palmyra fruit water + 10% egg yolk + 10% moringa leaf extract, T3 palmyra fruit water + 10% egg yolk + 15% moringa leaf extract, T4 palmyra fruit water + 20% egg yolk + 5% moringa leaf extract, T5 palmyra fruit water + 20% egg yolk + 10% moringa leaf extract, and T6 palmyra fruit water + 20% egg yolk + 15% moringa leaf extract. The semen was stored at a temperature of approximately 15-20°C for 48 hours. The observed semen quality parameters included motility, viability, and spermatozoa abnormalities, with each treatment repeated four times. The obtained data were analysed using ANOVA, followed by Duncan's test if significant differences were found. The results of Duncan's test indicated that the T6 treatment produced the best semen quality compared to other treatments, with motility at 59.50±1.291%, viability at 59.25±1.708%, and abnormalities at 7.25±0.500%. It can be concluded that the diluent formulation that resulted in the best spermatozoa quality after 48 hours of storage was treatment T6, composed of palmyra fruit water + 20% egg yolk + 15% moringa leaf extract. Further research is needed to determine the fertility potential of Landrace boar semen diluted with palmyra fruit water extender with the addition of various concentrations of egg yolk and moringa leaf extract to evaluate the effectiveness of the extender in an artificial insemination program.