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Mohamad Endy Y Yulianto
Departement Of Chemical Engineering, Vocational School, Diponegoro University
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Journal : Journal La Multiapp

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Simultaneous Caffeine Extraction and Enzymatic Inactivation through Microwave Thermobiochemical Process Yulianto, Mohamad Endy; Madina, Haura Salwa; Aprilani, Berliana
Journal La Multiapp Vol. 6 No. 3 (2025): Journal La Multiapp
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallamultiapp.v6i3.2238

Abstract

Indonesia is known as one of the countries producing tea with high bioactive compounds such as caffeine, phenol, and catechin. This study evaluated the effectiveness of microwave-based thermobiochemical methods to extract the three compounds while simultaneously inactivating enzymes. The extraction process was designed using the Factorial Design method, involving variations in the feed:solvent ratio (1:22 and 1:32), temperature (110 °C and 130 °C), and time (10, 20, and 30 minutes). The results showed that catechin had the highest effect of 5.85 with a probability of 35.87%, followed by phenol (5.18; 59.68%) and caffeine (4.51; 54.47%). The normal probability plot graph indicated a distribution of effects approaching normal with R² of 0.9458 (catechin), 0.9395 (phenol), and 0.7946 (caffeine), respectively. Meanwhile, the optimization graph shows a perfect linear relationship (R² = 1), with optimal levels of 17.18 mg/L (catechin), 9.36 mg/L (phenol), and 9.77 mg/L (caffeine), respectively. Thus, this method is proven to be efficient and has the potential to be further developed for sustainable natural material processing industry applications. The variables were designed using the Factorial Design method.
Co-Authors Abdullah Ridhwan Purnomo Adisti Putri Intaniasari Andi Nur Alamsyah Anggista, Giovani Anggun Puspitarini Siswanto Anindya Ardiansari Aprilani, Berliana Ari Yuniastuti Astuti, Septi Kusuma Ayun Sriatmi Azhara, Belinda B Budiyono Broto, R. T. D. Wisnu D. K. wikanta Damayani, Intan Ardina Deddy Kurniawan Wikanta Devita Hardianti Didik Ariwibowo Didik Ariwibowo Diwiyanto, Yusron Mahendra Diyono Ikhsan Diyono Ikhsan Dwi Handayani Dwi Handayani Dwi Handayani Edy Supriyo Eflita Yohana Endah Lestari Fahmi Arifan Fardzanela S Fardzanela S Fauzi, Nurrohmat Fiqih Putri Juanita Frisca Riana A FS Nugraheni S Hanifah Hanifah Heny Kusumayanti Heny Kusumayanti Hermawan Dwi Ariyanto Hermawan Dwi Ariyanto I. Hartati Ifana Ifana Ilyas Teguh Pangestu Indah Hartati Indah Hartati Indra Waspada Isti Pudjihastuti Juli Mrihardjono Juli Mrihardjono Latif, Abdul Madina, Haura Salwa Mandy Ayulia Dwisukma Margaretha Tuti Susanti Margaretha Tuti Susanti Monawisya, Abitha Muhammad Rizki Mulyaningsih, M.F. Sri Mustikaningrum, Mega Nanda, Dodi Eko Nanik Damayanti Nisa, Qurrotun A'yuni Khoirun Nyamiati, Retno Dwi Oktavian Dita Ratnasari Pangestu, Ilyas Teguh Pramudika, Satya Prasakti, Laras Priyono Kusumo R.T.D Wisnu Broto Rangkum Sembodo Rendiyatna Ferdian Riana Sitawati Riana Sitawati Rizka Amalia Rizka Amalia Rizka, Rashinta RTD Wisnu Broto Rudi Firyanto Saputri, Arum Dyah Saputri, Rizky Yunita Selvina Selvina Senen Senen Seno Darmanto Seno Darmanto Siti Nur Jannah Sovia, Ghina Sri Utami H Sri Utami Handayani Susiana Purwantisari Sutrisno Sutrisno Sutrisno, Sutrisno Sutrisno, Sutrisno Tandang Patria Tama Udin Bahrudin, Udin Utari, Febiani Dwi Vita Paramita Vita Paramita Vita Paramitha Wahyuningsih Wahyuningsih Wendi E Wendi E Winda Kristiana Yudanto, Yusuf Arya Zainal Abidin Zainal Abidin Zainal Abidin